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InvisibleWall.E
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Re: Psilocybe natalensis [Re: Grimsweeper]
    #27407541 - 07/29/21 05:24 PM (2 years, 5 months ago)

Quote:

Grimsweeper said:
Quote:

Wall.E said:
Is there anyone out there at all that can send specimens to Alan or someone of similar like? I thought we decided nss and natalensis are the same thing and it would be nice to have that confirmed



I sent a natalensis sample to Alan. We went back and forth a few times over months and I got bored asking about it.




I’ll ask him, I ain’t got shit going on


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Life’s shit, but I’m loving it



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OfflineLostkeys
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Re: Psilocybe natalensis [Re: smalltalk_canceled]
    #27407800 - 07/29/21 09:01 PM (2 years, 5 months ago)

Quote:

smalltalk_canceled said:
Yes I'm saying it. Lost radio contact two hours ago, that's usually a good sign.

They Ate 38g fresh blob / overlay based on the usual fresh10x rule of thumb



At the rate of potency going around for these things, that's a heroic dose. Are these travelers alright?


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OfflineFridgedoor
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Re: Psilocybe natalensis [Re: Lostkeys]
    #27408002 - 07/30/21 12:32 AM (2 years, 5 months ago)

I wonder about that too.

I had a 2 gram tea last night and that sent me to the dark side of the moon.


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Invisiblealaskappalachian
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Re: Psilocybe natalensis [Re: Fridgedoor] * 1
    #27408050 - 07/30/21 02:00 AM (2 years, 5 months ago)

God y'all got me excited to eat these again.  Finally have the house to myself this whole weekend and have plans for a 3g tea. :omgawesome:  Thinking about setting up a tent by the creek Saturday night and getting weird.  :spaceman:


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THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: alaskappalachian]
    #27408159 - 07/30/21 07:20 AM (2 years, 5 months ago)

Say hi to Bigfoot for me.




I really need to move out of the city again, camping by a nice quiet creek whenever I want sounds about right.


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LAGM2020


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OfflinePitcherCrab
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Re: Psilocybe natalensis [Re: alaskappalachian]
    #27408189 - 07/30/21 08:00 AM (2 years, 5 months ago)

Quote:

alaskappalachian said:
God y'all got me excited to eat these again.  Finally have the house to myself this whole weekend and have plans for a 3g tea. :omgawesome:  Thinking about setting up a tent by the creek Saturday night and getting weird.  :spaceman:




Nice! I'm excited to hear your report!


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineSolipsis
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Re: Psilocybe natalensis [Re: gone-pear-shaped]
    #27408646 - 07/30/21 04:00 PM (2 years, 5 months ago)

Quote:

gone-pear-shaped said:
Quote:

A.k.a said:
Something obvious like an albino or rust spores is probably the place to start to see how likely they are to cross.




What's the advantage of that? It could be a cross and not rust/albino, or it could be rust/albino and not a cross. Or am I misunderstanding something? How is rust spores or albino different than other non-natalensis characteristics?




Because its so obvious / unmistakable. Many traits can be kind of vague, gradual, subtle such as small differences in shape but rustybrown vs dark purple spores thats a discrete step between them. It seems like in Pans the spore pigments work differently genetically and if you cross strains (/species even) with different color spores then it is possible to get a result with a color something in between. With cubes that is not normal although idk exactly everything that goes on in the golden halo / azrael (sp?) eye of sauron kind of prints.

When you are not quite sure of whats going on when it comes to crossing or breeding etc, it is smart to start with leucists / rusty spored ones (or leucistic rusty spored ones) so that you can better track the result. These traits are expected to skip a generation, but still - trust me, it makes things a lot easier to become more certain of.

Anyway, like ive said before I wasn't able to cross NSS with a random cube or NSS with natalensis at least not with di-mono mating, but of course it is possible I messed something up. It's real cool that someone else pulled it off.

Now i gotta dig thru this thread to try to confirm what I think which is: that NSS is a bitch to tell apart for sure from natalensis. I got both and just wanna get certainty that my natalensis really is that, so it would be great do double check before I go and do barcode sequencing on it if i can afford it.

By the way I am pretty sure that Alan got a confirmed natalensis. I don't know if he also has NSS, I just heard it as a rumor from a friend that in general the existence of NSS as an additional cube had also been confirmed but he didn't say by whom. I've seen the natalensis ITS but am just not allowed to share it. I could share my own IF i get mine sequenced and IF it actually does come back natalensis.

It also seems like you could never 100% prove that the cube NSS exists in the sense that people can always say "what makes you so certain that this cube you have is NSS and came from the wild" except for a couple things: that you could just limit it to what exactly is being sold in South Africa as NSS and could sequence that - tho if i understood my friend correctly, it sounded like thats already been done, but besides that... what also supports both existing is this cube NSS having most of the appearance of natalensis namely half pale cap, very very lanky stipes, but however with pronounced veil which natalensis is not supposed to have.
So that makes it not just any random cube that was found that got an NSS label slapped on.

I need to inspect my own photos of "natalensis" again but while there was indeed little veil to speak of iirc, i don't think it was 100% absent - that part makes me suspicious, its way more vague than i care for.


Edited by Solipsis (07/30/21 04:23 PM)


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Solipsis]
    #27408672 - 07/30/21 04:32 PM (2 years, 5 months ago)

I think the veil thing was just from a lack of phenos when they documented it.

I’ve had tubs where most have veils halfway down the stem and then half seem to not have one.

Ime veils are more common than not.


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Offlinesmalltalk_canceled
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Re: Psilocybe natalensis [Re: alaskappalachian]
    #27408677 - 07/30/21 04:34 PM (2 years, 5 months ago)

Cross it with a non blob I'd say
Quote:

alaskappalachian said:
God y'all got me excited to eat these again.  Finally have the house to myself this whole weekend and have plans for a 3g tea. :omgawesome:  Thinking about setting up a tent by the creek Saturday night and getting weird.  :spaceman:




It lasted around 4-5 hours. Reading his drivel of a report now. My take is that the mycelium is less active than the fruits, but it's still quite active

It's funny to give people alien mycelium and have them eat it!
Remember giving a guy a jar of subtropica grain myc, telling him to boil it and drink the liquid, also worked but not a full trip according to guina pig

This natalensis myc is definitely more potent and can give big trips with tons of visuals

However I'd like to start one additional discussion:

How do you guys dry or prep highly active mycelium?

- how long can it keep in a food store box in the refrigerator
- can you still dry, and cook tea from it if it goes bad
- can you freeze it
- just dry regularly, mycelium "crackers" ?


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Willpower is the one true virtue



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Offlinejomanda1990
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Re: Psilocybe natalensis [Re: smalltalk_canceled]
    #27408709 - 07/30/21 04:59 PM (2 years, 5 months ago)

hmm how would one correclty dose myc that is stuck to grain in jars?
At least with overlay you can separate it from the sub easily.

Another variable that could matter in terms of overlay potency is how long the overlay was left to develop/consolidate before harvest. Maybe some controlled experiments with clone cultures are due.

I'll be ready to spawn some shoeboxes in 5 or 6 days when I get back home. I'm really excited to see how it goes.
Recommended spawn ratio is 1:3 using field capacity CVG as substrate?


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Offlinesmalltalk_canceled
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Re: Psilocybe natalensis [Re: jomanda1990]
    #27408720 - 07/30/21 05:09 PM (2 years, 5 months ago)

Quote:

jomanda1990 said:
hmm how would one correclty dose myc that is stuck to grain in jars?
At least with overlay you can separate it from the sub easily.

Another variable that could matter in terms of overlay potency is how long the overlay was left to develop/consolidate before harvest. Maybe some controlled experiments with clone cultures are due.

I'll be ready to spawn some shoeboxes in 5 or 6 days when I get back home. I'm really excited to see how it goes.
Recommended spawn ratio is 1:3 using field capacity CVG as substrate?




For this specific subtropica jar, I knew the amount of grains amounted to 70-80g so I figured that the myc would be a okay dose regardless.

The "10 rule" is the only thing I can think of to help, that fresh usually reflects dried 10x roughly


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: smalltalk_canceled]
    #27408762 - 07/30/21 06:27 PM (2 years, 5 months ago)

I don’t think anybody has experience with processing myc.

A while back I did peel off a bunch of overlay and put it in the dehydrator but never ate it. It dried into these chips with a kind of circus peanut type squish to them.


It had blue streaks in it too dried, looks pretty cool.


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Invisiblemycosavant

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Re: Psilocybe natalensis [Re: Wall.E]
    #27408787 - 07/30/21 07:00 PM (2 years, 5 months ago)

Quote:


Wall.E said:
Is there anyone out there at all that can send specimens to Alan or someone of similar like? I thought we decided nss and natalensis are the same thing and it would be nice to have that confirmed






I've talked to alan about it and he's the one the told me there were 6 differences between the two compared against GENBANK sequences. He has personally sequenced Ps. natalensis but not a specimen labeled var NSS. I'm like 90% sure he has both. Definitely has nat since that's the origin of my prints. He said he's going to send me the sequence.

on a side note, does anyone from this group have a natalensis print to spare? I just want to be sure we're working from the same pallete here.

once i get it going I'll sequence it myself and post the results.


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Offlinegone-pear-shaped
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Re: Psilocybe natalensis [Re: mycosavant]
    #27409029 - 07/30/21 10:15 PM (2 years, 5 months ago)

Are you international? I think nobody in the US wants a print from me due to month+ shipping time. But recently I got a thick dark nat print from a member of this thread, and it's more spores than I could ever need. I can make extra swabs for myself and pass the print on.


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OfflineFridgedoor
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Re: Psilocybe natalensis [Re: gone-pear-shaped]
    #27409132 - 07/31/21 01:43 AM (2 years, 5 months ago)

I could make a fresh print too. But I too am not based in the US. I stay in South Africa a country with a slow post office. Let me know if I can help though.


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OfflineBrownBear
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Re: Psilocybe natalensis [Re: Fridgedoor]
    #27409575 - 07/31/21 01:03 PM (2 years, 5 months ago)

T1 plate. Made a couple transfers to lc. Its been a while since I have ran any lc. So I am a little excited to get back into it.



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OfflinePrimalSoup
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Re: Psilocybe natalensis [Re: smalltalk_canceled]
    #27409926 - 07/31/21 07:15 PM (2 years, 5 months ago)

Quote:

smalltalk_canceled said:
This natalensis myc is definitely more potent and can give big trips with tons of visuals




:holyshitbubbles:

Yeah I would just make tea from the active myc and freeze it.  That could be a really cool route for this stuff.




This is a germ plate about 5 weeks old set aside for 2 weeks after it had gone to grain (that contamed out). 



I would point out in left pic the pins appear almost "Alien" like in that they are encased in a myc latticework.  Maybe that's what this overlay thing is about, trying to establish correct micro-climate for supporting pins?  Admittedly that's a big extrapolation, but it kinda looks like a spider has been at work on that plate. :laugh2:

But seems like if it'll pin directly on agar without any fuss some sub more like agar in nute value might make it happy. :buzzaldrin:


--------------------

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Offlinesmalltalk_canceled
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Re: Psilocybe natalensis [Re: PrimalSoup]
    #27409936 - 07/31/21 07:25 PM (2 years, 5 months ago)

Let me see radical casings guys

Theory: It's looking for something.

What do African natalensis fruit on in the wild?


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Willpower is the one true virtue



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Offlinegone-pear-shaped
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Re: Psilocybe natalensis [Re: smalltalk_canceled]
    #27410128 - 08/01/21 12:10 AM (2 years, 5 months ago)

My first two tubs pinned with no overlay. After only 11 days! The stats:

Mycelium was fast running rhizo strain without aerials agar (but the substrate quickly filled with aeril sprouts that covered the surface, then the little remaining surface became very slightly fuzzy). Grain was popcorn. Spawned 1:2 to coir with a thick top layer (probably 0.7-1 cm, but I didn't measure). Top layer was part of the 2 parts coir, not additional. Shoebox and grain colonization were fast--I'd say this strain is faster than the other I tried. I don't remember whether I unblocked holes in the tub to give lots of FAE, but probably did. But not for the whole time. I think I unblocked the holes when it looked fully colonized, and covered them loosely when I saw beads of water on the surface being small, not looking nearly as wet as cube substrate always looks in my humidity.

The aerials gained a tint of yellow a few days before pinning--the tops of the mycelia are off-white, not pure white like cubensis.

IMO since corn is mostly water, the 1:2 ratio is equivalent to a 1:3 ratio with denser grains.


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Invisiblealaskappalachian
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Re: Psilocybe natalensis [Re: gone-pear-shaped]
    #27410148 - 08/01/21 12:35 AM (2 years, 5 months ago)

Yeah my projects with minimal overlay were with thinner rhizo, and it would seem that a thicker top layer is very helpful. They also tend to respond to bacteria by overlaying heavily and (let's call it blobbing).  Not a huge surprise but thought it was interesting.


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


Edited by alaskappalachian (08/01/21 03:34 AM)


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