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OwlBundy
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Humidifier with hydrogen peroxide.
#26414237 - 01/03/20 11:54 AM (4 years, 26 days ago) |
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After spawning to substrate I have been getting contams even with mostly coir as substrate. Ive read that hydrogen peroxide kills spores but not mycelium. If I mixed hydrogen peroxide in a humidifier to provide some air flow + humidity would this help, harm, or do nothing at all?
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trubblesome
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414248 - 01/03/20 12:03 PM (4 years, 26 days ago) |
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to quote bod, "hydrogen peroxide has no place in mycology"
get your spawn right
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feldman114
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414251 - 01/03/20 12:05 PM (4 years, 26 days ago) |
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Spores aren’t your problem. It’s nearly impossible to germinate spores on coir.
Peroxide has no place in this hobby, with one exception - cobweb mold - but it doesn’t even work for that most of the time.
People here will help you get to the root of your problem, but you gotta provide more info and, if you want a lot of replies, PICS! First thing first, how do you prepare spawn? What did you inoculate with? And what’s your sterile tek like during inoculation? 99% of contams seem to stem from dirty spawn...
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OwlBundy
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Re: Humidifier with hydrogen peroxide. [Re: feldman114]
#26414271 - 01/03/20 12:20 PM (4 years, 26 days ago) |
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Okay Ive been using old books and winging it for years on outdated info but ill give rundown. I mix 4% honey 96% water and inoculated from spores. Prepare WBS by pouring boiling water in a cooler and drain after an hour. Rinse 4-5 times then let drain for an hour. Inoculate pint jars with lc. Just aluminum foil lid with single hole covered in micropore. Use an aquarium for SAB and individually bag in ziplocs with hole for micropore tape. It takes about 3 weeks to colonize and I rarely get contams in the spawn. I just seem to get when mixing with substrate. I prepare coir same way as WBS. I have young ones so my grow room is in a terrible place but I enjoy the hobby and am trying to make it work.
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feldman114
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414274 - 01/03/20 12:21 PM (4 years, 26 days ago) |
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Wait wait wait whaaaaaat???? You made an lc using a spore syringe? Dude.
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feldman114
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Re: Humidifier with hydrogen peroxide. [Re: feldman114]
#26414280 - 01/03/20 12:27 PM (4 years, 26 days ago) |
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Okay, so ima let someone else weigh in on the stuff I’m not 100% sure on but...
1.Coir doesn’t need to be prepped like WBS, though that doesn’t hurt you. You can sterilize it, pour boiling water on it, or just add tap water.
2. How is your SAB an aquarium? Did you use glass cutters to make armholes? If you’re just flipping it so 1 whole side is open, the air inside it won’t be very still.
3. VISIBLE contams tend to rarely show up in jars, but they’re there. Pics would help, but what would really help is starting with agar. If you had success with LC, agar will be a piece of cake.
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OwlBundy
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Re: Humidifier with hydrogen peroxide. [Re: feldman114]
#26414281 - 01/03/20 12:27 PM (4 years, 26 days ago) |
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Whats wrong with that? Ive had some grow before using Lc inoculated from spores. Whats the correct way?
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OwlBundy
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414285 - 01/03/20 12:29 PM (4 years, 26 days ago) |
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I put the aquarium on the side and cut an area big enough for hands out of plastic top that fits the aquarium. Sealed off any holes. I dumped the monotub out last night because it had a bad smell. I can try to get a pic of jars I inoculated with lc
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feldman114
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414286 - 01/03/20 12:30 PM (4 years, 26 days ago) |
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Quote:
OwlBundy said: Whats wrong with that? Ive had some grow before using Lc inoculated from spores. Whats the correct way?
You got very lucky, though I bet you grew some bacteria alongside your mushies anyway.
Spores aren’t sterile, so neither is the resulting myc. Using a cleaned up agar plate or clone tissue is the way to go. If an LC is dirty, you won’t know until you ruin a batch of jars. Meanwhile, you can literally see most contams on agar.
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Smartattack
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Re: Humidifier with hydrogen peroxide. [Re: feldman114]
#26414297 - 01/03/20 12:40 PM (4 years, 26 days ago) |
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You are also prepping grain in a cooler with hot water?
You've got at least 4 major errors that cause contamination going on here and always will until following known procedure. Get that down, then experiment. Just not these experiments...
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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OwlBundy
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414303 - 01/03/20 12:45 PM (4 years, 26 days ago) |
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Last run was WBS but this next grain is Oats also inoculated by lc from Treasure Coast instead of B+. Was hoping to find a solution before I lose these too. One doesnt even have a lid at all but seems to be colonizing fine. Black spots are coir at bottom of jar. Was testing to see if it reduce moisture.
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OwlBundy
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Re: Humidifier with hydrogen peroxide. [Re: Smartattack]
#26414309 - 01/03/20 12:48 PM (4 years, 26 days ago) |
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WBS is the only grain I prepare in the cooler because it overcooks boiling it. Its sterilized in pressure cooker anyway but ive had luck so far using this method.
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feldman114
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414368 - 01/03/20 01:18 PM (4 years, 26 days ago) |
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Quote:
OwlBundy said:

Last run was WBS but this next grain is Oats also inoculated by lc from Treasure Coast instead of B+. Was hoping to find a solution before I lose these too. One doesnt even have a lid at all but seems to be colonizing fine. Black spots are coir at bottom of jar. Was testing to see if it reduce moisture.
Ok well even with those sketchy quality pics, I can see that the jar is contaminated. The condensation-like moisture you see is not from over-hydrated grains. It’s usually a symptom of bacterial contams. The good news is, once you clean up your spawn, your yields (w.e you got from previous tubs) will at least double.
I’ve used WBS for years and settled on this prep:
1.Fill jars 1/2-full of dry WBS, add very hot tap water. 2. Wait 18-30 hours. 3. Rinse one jar’s worth of grain at a time under very hot tap water. 4. After rinse, spread grains out on an old towel, as thin as possible. 5. Wait 30-90min, then load jars.
Results are always consistent. Plus, forget about burst grains...(.this isn’t exactly “established” science, but I believe it) also, you’re letting all those pesky endo-spores germinate, so they die in the pc.
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WeavieWonder
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Re: Humidifier with hydrogen peroxide. [Re: feldman114]
#26414386 - 01/03/20 01:28 PM (4 years, 26 days ago) |
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The only reason to add peroxide to a humidifer is to reduce the build-up of gunk in the bottom. It will buy you more time between cleanings.
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feldman114
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Re: Humidifier with hydrogen peroxide. [Re: WeavieWonder]
#26414391 - 01/03/20 01:31 PM (4 years, 26 days ago) |
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Quote:
WeavieWonder said: The only reason to add peroxide to a humidifer is to reduce the build-up of gunk in the bottom. It will buy you more time between cleanings.
Huh, I guess there IS a use for it after all.
Though humidifiers themselves are completely unnecessary for cubensis monotub grows.
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WeavieWonder
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Re: Humidifier with hydrogen peroxide. [Re: feldman114]
#26414410 - 01/03/20 01:45 PM (4 years, 26 days ago) |
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Quote:
feldman114 said: humidifiers themselves are completely unnecessary for cubensis monotub grows.
They're nice to have around for certain situations. I live in a mountainous region in the desert. When I grow in the winter time, I have trouble keeping things properly moist while still maintaining proper FAE. Adding a humidifer to my grow area helps a lot.
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feldman114
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Re: Humidifier with hydrogen peroxide. [Re: WeavieWonder]
#26414459 - 01/03/20 02:15 PM (4 years, 26 days ago) |
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Quote:
WeavieWonder said:
Quote:
feldman114 said: humidifiers themselves are completely unnecessary for cubensis monotub grows.
They're nice to have around for certain situations. I live in a mountainous region in the desert. When I grow in the winter time, I have trouble keeping things properly moist while still maintaining proper FAE. Adding a humidifer to my grow area helps a lot.
In a mono? Do you use an unmodded tek? Hope that doesn’t sound like I don’t believe you or w.e, I just cant imagine how enough dry air can get inside through a poly/mp filter. Monos just seem so self-contained. What about shoeboxes? There’s not much clearance, so the evaporation goes a long way, right?
I live at sea level, so I wouldn’t know what it’s like to grow in low RH. For example, it’s been like this all week and it barely rained at all:
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wildernessjunkie
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Re: Humidifier with hydrogen peroxide. [Re: OwlBundy]
#26414525 - 01/03/20 02:52 PM (4 years, 26 days ago) |
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Quote:
I mix 4% honey 96% water and inoculated from spores.
Thats where your contamination is coming from.
Quote:
individually bag in ziplocs with hole for micropore tape.
Thats contributing to the problem.
If you have a PC then its probably time to start checking out the some of the "No Pour" Agar teks. Anytime you are using spore solution to inoculate a liquid or whole grain, you are going to end up with problems.
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WeavieWonder
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Re: Humidifier with hydrogen peroxide. [Re: feldman114]
#26414808 - 01/03/20 05:44 PM (4 years, 26 days ago) |
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Quote:
feldman114 said:
Quote:
WeavieWonder said:
Quote:
feldman114 said: humidifiers themselves are completely unnecessary for cubensis monotub grows.
They're nice to have around for certain situations. I live in a mountainous region in the desert. When I grow in the winter time, I have trouble keeping things properly moist while still maintaining proper FAE. Adding a humidifer to my grow area helps a lot.
In a mono? Do you use an unmodded tek? Hope that doesn’t sound like I don’t believe you or w.e, I just cant imagine how enough dry air can get inside through a poly/mp filter. Monos just seem so self-contained. What about shoeboxes? There’s not much clearance, so the evaporation goes a long way, right?
I live at sea level, so I wouldn’t know what it’s like to grow in low RH. For example, it’s been like this all week and it barely rained at all:

I've only got a couple mono-style grows under my belt. Definitely room for improvemnt. Ive always grown in mini green houses until recently.
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