|
Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 4 months
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: Kizzle]
#26413509 - 01/03/20 12:32 AM (4 years, 1 month ago) |
|
|
You are not wrong about MSS being inherently dirty. But that is insanely dirty. Like holy fuck dirty. If that was the norm, PFteks would never work. If you think it is worth it, maybe try to let vendor know there is an issue, without mentioning cultivation of course. I am a big proponent to agar and trying to "save" dirty cultures but this is one time I would just say screw it and get new\better syringes.
|
Growtech
Stranger

Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: Randalf the Grey]
#26413536 - 01/03/20 01:18 AM (4 years, 1 month ago) |
|
|
If that’s the normal bacteria I see in syringes, the bacteria will show wherever the syringe solution was within a day... but it won’t expand. Then when the mycelium germinates. It will overrun it. It will work out in a syringe to agar inoculation because the bacteria doesn’t expand really and the mycelium just overruns it. What you are seeing there is a pretty normal spore syringe in my experience. Wait a bit and you’ll have mycelium overrunning it I bet. Transfer away from bacteria and you can get clean mycelium pretty easy.
|
A.k.a
Stranger


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 16 hours, 1 minute
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: Growtech]
#26414364 - 01/03/20 01:15 PM (4 years, 1 month ago) |
|
|
Yeah I was just gonna say I had three plates similar to that, so much bac that it would run and pool up at the edge when I tilted the dish.
I left them in 40-50 degrees for a few days and idk if it was temp or time but when I came back the bacteria was mostly dried up and the myc had taken off and looked great.
Pretty lucky cuz I meant to toss them but got distracted packing and forgot.
--------------------
LAGM2020     
|
spiritlands



Registered: 06/21/10
Posts: 1,616
Loc:
Last seen: 2 months, 14 days
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: verum subsequentis]
#26414453 - 01/03/20 02:11 PM (4 years, 1 month ago) |
|
|
Quote:
verum subsequentis said: That is 100% coming from the syringe. Dirty as fuck. you may be able to save it but it's quite hard when they are that fucked.
If it is bacterial couldn't some alcohol be introduced to kill the bacteria but not harm the spores like peroxide would? I'm under the impression alcohol destroys the cell wall of bacteria but isn't antifungal.
|
verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: spiritlands]
#26414502 - 01/03/20 02:37 PM (4 years, 1 month ago) |
|
|
i really really doubt that would work.
|
Growtech
Stranger

Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: verum subsequentis]
#26414580 - 01/03/20 03:27 PM (4 years, 1 month ago) |
|
|
Antibiotics, hydrogen peroxide, alcohol, etc? The problem of a dirty syringe is being overthought. Wait a couple days, transfer some mycelium to a clean plate. If that transfer isn't clean in itself, do it once more.
|
naturalistic123



Registered: 02/18/18
Posts: 891
Last seen: 1 month, 7 days
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: verum subsequentis]
#26414583 - 01/03/20 03:29 PM (4 years, 1 month ago) |
|
|
Alchohol kills most bacteria, viruses and fungi on the spot.
-------------------- Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.
|
spiritlands



Registered: 06/21/10
Posts: 1,616
Loc:
Last seen: 2 months, 14 days
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: naturalistic123] 1
#26414640 - 01/03/20 03:57 PM (4 years, 1 month ago) |
|
|
Nevermind you're right. It was just an idea. I can clean most things on agar but there have been projects thrown out that would have been nice to save. This is from the center for disease control studies on chemical disinfectants.. There's lots of good info in this link.
Alcohol Overview. In the healthcare setting, “alcohol” refers to two water-soluble chemical compounds—ethyl alcohol and isopropyl alcohol—that have generally underrated germicidal characteristics 482. FDA has not cleared any liquid chemical sterilant or high-level disinfectant with alcohol as the main active ingredient. These alcohols are rapidly bactericidal rather than bacteriostatic against vegetative forms of bacteria; they also are tuberculocidal, fungicidal, and virucidal but do not destroy bacterial spores. Their cidal activity drops sharply when diluted below 50% concentration, and the optimum bactericidal concentration is 60%–90% solutions in water (volume/volume).
|
cryptowizard
Stranger


Registered: 12/05/19
Posts: 17
Last seen: 3 years, 10 months
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: spiritlands]
#26414663 - 01/03/20 04:19 PM (4 years, 1 month ago) |
|
|
Those were +2 weeks old and showing no signs of growth after the first 5-7 days, so I pitched them. I did a couple of transfers that grew out about 2-3x what the pic shows. I kept them, and just did 2 transfers of each (gen 3). They didn't look much better, but grew more than the others. I'll give the refrigerator trick a try in a couple of days with one of each to see if that works. Thank you for the suggestions.
I also setup some no-pour big lots PP rounds with filter discs melted to the side. I let them cool for a couple of days and did spore->agar x3 of each 3 strains again.
I only let the agar cool for a few hours in front of the flow hood (running) after pouring the first few times. Perhaps that has something to do with the contams being so bad. The plates smell like fermentation so perhaps it's yeast as someone suggested. I'll also repeat when the antibiotic MEA arrives.
|
cryptowizard
Stranger


Registered: 12/05/19
Posts: 17
Last seen: 3 years, 10 months
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: cryptowizard]
#26438290 - 01/17/20 10:31 AM (4 years, 30 days ago) |
|
|
Just a quick follow up - Got new syringes, did 4x of 5 strains and had zero contamination. I got some antibiotic MEA, but I'm not even going to bother with it. I'll save it for some wild collected spore prints. Thanks everyone.
|
Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 1 day, 5 hours
|
Re: Price Difference from a 99.97% to 99.99% HEPA Filter Worth It? [Re: cryptowizard]
#26438635 - 01/17/20 01:52 PM (4 years, 30 days ago) |
|
|
I got my 2'x4'×6" 99.997% @.3microns for like $210. The thickness isn't a problem, they're made to filter and they work.
|
|