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OfflineTakkaji
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I'm a teapot

Registered: 09/23/19
Posts: 113
Last seen: 4 years, 18 days
Old BRF cakes
    #26412784 - 01/02/20 03:32 PM (4 years, 1 month ago)

So uh.. I did an uh-oh...  I prepped 36 BRF half pints with the dry verm barrier and PC them all at 15 psi for almost 90 minutes.

They have 4 holes that are tiny in the lids and I read that the dry verm, being free of nutrients would prevent contamination. I was going to put spore syringe in them but remembered the horrors that resulted last time with my monotubs. So I put them in the freezer with the foil still on them. They've been there for about 2 months now, should they still be good?

I cant exactly use agar, so would a liquid culture of mycelium be possible? I would think the mycelium will drown in a liquid culture? I mean, it doesn't grow when sub is too damn wet after all..

Is there anyone who is experienced with Cakes and has their own proven method of inoculation?


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OfflineA.k.aM
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Registered: 10/27/19
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Re: Old BRF cakes [Re: Takkaji]
    #26413005 - 01/02/20 06:04 PM (4 years, 1 month ago)

Liquid culture is pretty popular. Might be perfect for this scenario since the cakes prob dried out some.

I don’t know for sure but it seems like they should be fine. Knocked up cakes can sit for months and months.


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LAGM2020


Edited by A.k.a (01/02/20 06:04 PM)


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