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OfflineMr_enforcer1
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Black mold on substrate!
    #26411651 - 01/01/20 10:47 PM (4 years, 1 month ago)

I’m fucking plagued with black mold on my shoeboxes, all of em have it and there’s nothing I can do to stop it it just keeps coming back. wtf do I do? Why is this happening




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Offlineazramb
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Re: Black mold on substrate! [Re: Mr_enforcer1]
    #26411671 - 01/01/20 11:14 PM (4 years, 1 month ago)

What's your substrate consist of? Nutrient rich substrates can be good for certain things but they are also much more prone to contamination.


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OfflineMr_enforcer1
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Re: Black mold on substrate! [Re: azramb]
    #26411690 - 01/01/20 11:33 PM (4 years, 1 month ago)

Coir only


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InvisibleAyePlusS
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Re: Black mold on substrate! [Re: Mr_enforcer1]
    #26411730 - 01/02/20 12:21 AM (4 years, 1 month ago)

Bad spawn. Sorry bud. Go back to agar and do some more transfers or swab some new spores


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OfflineMr_enforcer1
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Re: Black mold on substrate! [Re: AyePlus]
    #26411747 - 01/02/20 12:50 AM (4 years, 1 month ago)

It wasn’t bad spawn there were a few jars in question that all went in one tub and that one has trich. This keeps happening it’s not just this round of spawn and substrate and while it still fruits great it’s a pain in the ass to deal with and I want to figure why it’s happening and how to stop it.


This is what I’ve found but my temps are in the 70s I don’t think overheating is The problem

Black Whisker Mold %u2013 Doratomyces spp.

This fungus produces black powdery spores that appear as smoke when disturbed. This mold indicates the presence of certain carbohydrates in the compost at spawning time. It also indicates that the straw has been incompletely caramelized or underheated in Phase I (therefore, carbohydrates are in a form easily utilized). The proportion of carbohydrates, particularly cellulose, may be too high. The black whisker mold is also present in compost that overheated during spawn run. Simple carbohydrates are utilized by this fungus but it can also utilize lignin. Doratomyces, Aspergillus, and Penicillium produce copious numbers of spores and may cause respiratory problems (nasal and throat irritation, chest congestion, breathing difficulty, etc.).


https://www.shroomery.org/5276/What-are-common-contaminants-of-the-mushroom-culture


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InvisibleDiego


Registered: 12/30/19
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Re: Black mold on substrate! [Re: Mr_enforcer1]
    #26411905 - 01/02/20 05:38 AM (4 years, 1 month ago)

Revise your sterilization techinques


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Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineMr_enforcer1
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Re: Black mold on substrate! [Re: Diego]
    #26412023 - 01/02/20 07:42 AM (4 years, 1 month ago)

I wash my hands before touching the coir. I use the bucket tek


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Offlinefeldman114
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Re: Black mold on substrate! [Re: Mr_enforcer1]
    #26412031 - 01/02/20 07:49 AM (4 years, 1 month ago)

Looks like the stuff growing between the tiles of every old bathroom I’ve ever been in. Maybe you have a patch growing in the room? On the walls/ceiling maybe?


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OfflineMcDominator
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Re: Black mold on substrate! [Re: feldman114]
    #26412049 - 01/02/20 08:00 AM (4 years, 1 month ago)

Contamination comes from bad spawn. I grew tubs in a moldy basement with no contams. Spawn was clean, so tubs were clean.


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I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


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InvisibleDiego


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Posts: 346
Re: Black mold on substrate! [Re: Mr_enforcer1]
    #26412053 - 01/02/20 08:03 AM (4 years, 1 month ago)

Quote:

Mr_enforcer1 said:
I wash my hands before touching the coir. I use the bucket tek



Im saying the problem is in your procedure. Its not clean enough. Im not fimilar with the bucket tek. I get coir to feild capacity and boil it in a covered pot in qaurt jars for half an hour


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


Edited by Diego (01/02/20 08:04 AM)


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InvisibleAyePlusS
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Re: Black mold on substrate! [Re: McDominator]
    #26412114 - 01/02/20 08:53 AM (4 years, 1 month ago)

Quote:

McDominator said:
Contamination comes from bad spawn. I grew tubs in a moldy basement with no contams. Spawn was clean, so tubs were clean.



:whathesaid:

Its very unlikely its coming from your coir.

Lots of molds are hard to spot when theyre growing in grain.

Go back to agar.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
🅂 🄰 🄼 🄴  🅃 🄴 🄰 🄼
🅃 🄴 🄰 🄼  🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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OfflineA.k.aM
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Re: Black mold on substrate! [Re: AyePlus]
    #26412140 - 01/02/20 09:11 AM (4 years, 1 month ago)

Have you been using the same batch of coir for all the moldy tubs??

Based on that summary you posted it sounds like it’s usually from under heated straw, so I could see some robust mold hanging onto coir.

If it’s all been the same batch of coir I’d try pasteurization next time just out of curiosity.

If you’ve been growing fine and then all of a sudden mold pops up everytime it’s gotta be something consistent.

Maybe the syringe if that’s what you’re using? Seems like it’d show up before this point though.


I just reread it, it says it also grows on lignin isn’t that coir?


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LAGM2020


Edited by A.k.a (01/02/20 09:12 AM)


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InvisibleDiego


Registered: 12/30/19
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Re: Black mold on substrate! [Re: A.k.a]
    #26412174 - 01/02/20 09:28 AM (4 years, 1 month ago)

Quote:

A.k.a said:
Have you been using the same batch of coir for all the moldy tubs??

Based on that summary you posted it sounds like it’s usually from under heated straw, so I could see some robust mold hanging onto coir.

If it’s all been the same batch of coir I’d try pasteurization next time just out of curiosity.

If you’ve been growing fine and then all of a sudden mold pops up everytime it’s gotta be something consistent.

Maybe the syringe if that’s what you’re using? Seems like it’d show up before this point though.


I just reread it, it says it also grows on lignin isn’t that coir?




I agree with you here and yes it is.


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineA.k.aM
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Re: Black mold on substrate! [Re: Diego]
    #26412182 - 01/02/20 09:32 AM (4 years, 1 month ago)

I just looked at that contamination page and if you scroll past the section you posted and find thr aspergillus part there’s a black version that looks similar also.


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InvisibleDiego


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Re: Black mold on substrate! [Re: A.k.a]
    #26412221 - 01/02/20 09:58 AM (4 years, 1 month ago)

This is the reason i quit using coir as a casing also


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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OfflineMr_enforcer1
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Re: Black mold on substrate! [Re: A.k.a]
    #26412317 - 01/02/20 10:55 AM (4 years, 1 month ago)

Quote:

A.k.a said:
Have you been using the same batch of coir for all the moldy tubs??

Based on that summary you posted it sounds like it’s usually from under heated straw, so I could see some robust mold hanging onto coir.

If it’s all been the same batch of coir I’d try pasteurization next time just out of curiosity.

If you’ve been growing fine and then all of a sudden mold pops up everytime it’s gotta be something consistent.

Maybe the syringe if that’s what you’re using? Seems like it’d show up before this point though.


I just reread it, it says it also grows on lignin isn’t that coir?






This makes sense, I don’t boil the water I use hot tap water around 130F this is most likely the culprit, thank you


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OfflineA.k.aM
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Re: Black mold on substrate! [Re: Mr_enforcer1]
    #26412320 - 01/02/20 10:57 AM (4 years, 1 month ago)

Right on I hope it is.

I think most people just use hot water in a bucket. If this does turn out to be the problem you should come back and let us know what brand it was.


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OfflineMr_enforcer1
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Re: Black mold on substrate! [Re: A.k.a]
    #26412332 - 01/02/20 11:07 AM (4 years, 1 month ago)

Will do :thumbup:


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