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OfflineeLeSDenes
Mycelium Expander
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Folding@home Statistics
Registered: 05/25/11
Posts: 955
Last seen: 2 years, 7 days
Re: Let's grow some mold [Re: Luminous7]
    #26412248 - 01/02/20 10:14 AM (4 years, 1 month ago)

let me join the club :laugh:



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Offlinefootpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 3 months
Re: Let's grow some mold [Re: eLeSDenes]
    #26413037 - 01/02/20 06:25 PM (4 years, 1 month ago)

Got some cubensis 'bout to sporulate all over my mold :crankey:



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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
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Re: Let's grow some mold [Re: footpath]
    #26413040 - 01/02/20 06:26 PM (4 years, 1 month ago)

Lol bastards!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleShamanBoof
Undergraduate EECS
Male


Registered: 08/25/19
Posts: 131
Loc: France Flag
Re: Let's grow some mold [Re: cronicr]
    #26416960 - 01/05/20 02:22 AM (4 years, 1 month ago)

Wtf! I thought mold was easy to kill by heat  :cryariver:
Remember this jar I shared a picture of

I PCed for 1 hour to reuse the jar then decided to crumble the grains and PC for about 2 hours. I did not touch the jar after it's been sterilized for the second time, to my surprise one week later green is back!


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OfflineHolyBolete
Mold Slayer
I'm a teapot User Gallery


Registered: 12/15/19
Posts: 263
Loc: USA 🇺🇸
Last seen: 3 years, 6 months
Re: Let's grow some mold [Re: ShamanBoof]
    #26416965 - 01/05/20 02:32 AM (4 years, 1 month ago)

Quote:

ShamanBoof said:
Wtf! I thought mold was easy to kill by heat  :cryariver:
Remember this jar I shared a picture of

I PCed for 1 hour to reuse the jar then decided to crumble the grains and PC for about 2 hours. I did not touch the jar after it's been sterilized for the second time, to my surprise one week later green is back!





Could be problem with Vendor spore syringe, inoculating in an open environment, combo of poor air quality and a lid holes with no filter protection, endospores,... gotta trace back and cross eliminate these areas to know for sure.


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InvisibleShamanBoof
Undergraduate EECS
Male


Registered: 08/25/19
Posts: 131
Loc: France Flag
Re: Let's grow some mold [Re: HolyBolete]
    #26416996 - 01/05/20 03:23 AM (4 years, 1 month ago)

Quote:

HolyBolete said:
Quote:

ShamanBoof said:
Wtf! I thought mold was easy to kill by heat  :cryariver:
Remember this jar I shared a picture of

I PCed for 1 hour to reuse the jar then decided to crumble the grains and PC for about 2 hours. I did not touch the jar after it's been sterilized for the second time, to my surprise one week later green is back!





Could be problem with Vendor spore syringe, inoculating in an open environment, combo of poor air quality and a lid holes with no filter protection, endospores,... gotta trace back and cross eliminate these areas to know for sure.



I did not inoculate it nor open it.. 100% the mold did survive the 2hrs inside the PC


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InvisibleGreen_Hands
The Great Devourer
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Registered: 05/21/05
Posts: 625
Loc: Old World
Re: Let's grow some mold [Re: ShamanBoof]
    #26417009 - 01/05/20 03:45 AM (4 years, 1 month ago)

Filter is not good. Mold cant survive pc.


--------------------


Looping our reflections, our obsessions draw us in
Fix and fixation, no sentience beyond


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OfflineHolyBolete
Mold Slayer
I'm a teapot User Gallery


Registered: 12/15/19
Posts: 263
Loc: USA 🇺🇸
Last seen: 3 years, 6 months
Re: Let's grow some mold [Re: ShamanBoof]
    #26417011 - 01/05/20 03:48 AM (4 years, 1 month ago)

Quote:

ShamanBoof said:
Quote:

HolyBolete said:
Quote:

ShamanBoof said:
Wtf! I thought mold was easy to kill by heat  :cryariver:
Remember this jar I shared a picture of

I PCed for 1 hour to reuse the jar then decided to crumble the grains and PC for about 2 hours. I did not touch the jar after it's been sterilized for the second time, to my surprise one week later green is back!





Could be problem with Vendor spore syringe, inoculating in an open environment, combo of poor air quality and a lid holes with no filter protection, endospores,... gotta trace back and cross eliminate these areas to know for sure.



I did not inoculate it nor open it.. 100% the mold did survive the 2hrs inside the PC



Didn't know that was possible, so absolutely no entry points on jar and growing without inoculation?

:pipesmoke:


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InvisibleGreen_Hands
The Great Devourer
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Registered: 05/21/05
Posts: 625
Loc: Old World
Re: Let's grow some mold [Re: HolyBolete]
    #26417013 - 01/05/20 03:49 AM (4 years, 1 month ago)

They cant. Its filter failure.


--------------------


Looping our reflections, our obsessions draw us in
Fix and fixation, no sentience beyond


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OfflineHolyBolete
Mold Slayer
I'm a teapot User Gallery


Registered: 12/15/19
Posts: 263
Loc: USA 🇺🇸
Last seen: 3 years, 6 months
Re: Let's grow some mold [Re: Green_Hands]
    #26417014 - 01/05/20 03:53 AM (4 years, 1 month ago)

:whathesaid:


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InvisibleShamanBoof
Undergraduate EECS
Male


Registered: 08/25/19
Posts: 131
Loc: France Flag
Re: Let's grow some mold [Re: Green_Hands]
    #26417041 - 01/05/20 04:53 AM (4 years, 1 month ago)

Quote:

Green_Hands said:
Filter is not good. Mold cant survive pc.




I changed the lid and the filter after the first fail, tomorrow gonna make some LC and grains i'll leave it to cook with the LC for 30min then i'll change the lid and filter and cook it for 2h with the grains. No fucking chance for 3 different filters to fail in raw.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
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Re: Let's grow some mold [Re: ShamanBoof]
    #26417484 - 01/05/20 12:28 PM (4 years, 1 month ago)

Yep it's failing you somewhere , lid I
Or filter. Just dump it out and start over


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineHolyBolete
Mold Slayer
I'm a teapot User Gallery


Registered: 12/15/19
Posts: 263
Loc: USA 🇺🇸
Last seen: 3 years, 6 months
Re: Let's grow some mold [Re: cronicr]
    #26417496 - 01/05/20 12:35 PM (4 years, 1 month ago)

Quote:

ShamanBoof said:
Quote:

Green_Hands said:
Filter is not good. Mold cant survive pc.




I changed the lid and the filter after the first fail, tomorrow gonna make some LC and grains i'll leave it to cook with the LC for 30min then i'll change the lid and filter and cook it for 2h with the grains. No fucking chance for 3 different filters to fail in raw.



Quote:

cronicr said:
Yep it's failing you somewhere , lid I
Or filter. Just dump it out and start over




Just go with the standard two piece ball lid on a ball jar.

At least to see if it's your filter or lid you can just boil one jar completely sealed/pc'd w no holes on top (regular unmodified lid).

If that jar becomes contaminated then there is some problem with your PC or just that the universe hates you.


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OfflineSamskara92
Certified Hot Dropper
I'm a teapot User Gallery


Registered: 08/29/19
Posts: 334
Loc: Pochinki
Last seen: 8 days, 19 hours
Re: Let's grow some mold [Re: HolyBolete]
    #26426492 - 01/10/20 01:01 PM (4 years, 1 month ago)


:crankey:


--------------------
:jellyfish: We're all just Motion in the Dust:jellyfish:


LAGM 2020 Grow Log - KSSS
CBK's Proper Surface Moisture/ FAE Tek


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,392
Loc: where?
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Re: Let's grow some mold [Re: Samskara92]
    #26426512 - 01/10/20 01:12 PM (4 years, 1 month ago)


:crankey::crankey:


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OfflineNikoyo
FUN GUY
I'm a teapot


Registered: 01/29/17
Posts: 289
Last seen: 5 days, 4 hours
Re: Let's grow some mold [Re: mushboy]
    #26426888 - 01/10/20 04:44 PM (4 years, 1 month ago)


Shit happens when you party naked:shrug::gum:


--------------------
Gettin back to work


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Invisiblestaytrippy420
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Registered: 03/23/13
Posts: 2,337
Loc: Canada Flag
Re: Let's grow some mold [Re: Nikoyo]
    #26453336 - 01/26/20 11:10 AM (4 years, 22 days ago)



Dusty ass RW germ plate decided to pin 😈


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OfflineSockadin
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Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
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Re: Let's grow some mold [Re: Green_Hands]
    #26453350 - 01/26/20 11:17 AM (4 years, 22 days ago)

So for years I was using the plastic reusable lids from Ball... I was constantly having spawning contamination issues, with a Expensive Hepa Filter and also using a SAB when I was to lazy to turn on the Hepa....  Finally I realized the reusable plastic lids SUCK. I have recently switched back to the Metal lids and had good success so far.


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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 2 hours, 52 minutes
Trusted Cultivator
Re: Let's grow some mold [Re: Sockadin]
    #26453360 - 01/26/20 11:23 AM (4 years, 22 days ago)

I've been thinking about those plastic Ball lids too and switching to one piece metal lids.

You can test those plastic lids by filling up a quart jar with water and
turning it upside down. Half of my plastics lids leak like a goddamn
sieve.


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Invisiblegizmo1
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Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM Flag
Re: Let's grow some mold [Re: Asura]
    #26453391 - 01/26/20 11:43 AM (4 years, 22 days ago)

I been thinking about buying some gaskets for my plastic lids. I wrap them(gap between jar and threads) either in masking tape or parafilm after inoculation especially if I plan to use as a master. I feel like the gasket would save me time and supplies. I just hate metal lids and already have a shit ton of plastic lids.


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