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North Spore Shop: Cultivation Supplies

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Offlinemagicschoolbus
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Registered: 02/19/18
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Pouring new agar on top of unused agar?
    #26412214 - 01/02/20 09:48 AM (4 years, 1 month ago)

I had made up 20 plates the other day but they're all full of tiny little bubbles. I'm reluctant to use the plates because I want my plates to be smooth and clear.

I also decided I should start using food dye since my agar pours so clear, and I found black food dye that I'm excited to use and don't want the 20 bubbly plates to go to waste.

So I was thinking I'd just pour a new layer of the black agar over top of the bubbly agar... don't see why this would be an issue besides a little extra thickness, right? The plates were poured about 4 days ago in front of a flow hood and don't have anything growing on them, so I figure the worst that could happen would be a little pasteurization from the new pour


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InvisibleSpunkyMonkey88
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Registered: 10/08/19
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Re: Pouring new agar on top of unused agar? [Re: magicschoolbus]
    #26412218 - 01/02/20 09:54 AM (4 years, 1 month ago)

Quote:

magicschoolbus said:
I had made up 20 plates the other day but they're all full of tiny little bubbles. I'm reluctant to use the plates because I want my plates to be smooth and clear.

I also decided I should start using food dye since my agar pours so clear, and I found black food dye that I'm excited to use and don't want the 20 bubbly plates to go to waste.

So I was thinking I'd just pour a new layer of the black agar over top of the bubbly agar... don't see why this would be an issue besides a little extra thickness, right? The plates were poured about 4 days ago in front of a flow hood and don't have anything growing on them, so I figure the worst that could happen would be a little pasteurization from the new pour




I would definitely test it on a plate first and let it cool before you pour a whole stack...

Might do something funny and itll obviously be on the thick side... doubt it will fix your original problem with the bubbles


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InvisibleBph
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Registered: 10/11/18
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Re: Pouring new agar on top of unused agar? [Re: magicschoolbus]
    #26412220 - 01/02/20 09:56 AM (4 years, 1 month ago)

It will probably work but the myc don't care about bubbles so I'd just use the plates and try to avoid the bubbles next time.


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Offlinespiritlands
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Re: Pouring new agar on top of unused agar? [Re: Bph]
    #26412225 - 01/02/20 10:03 AM (4 years, 1 month ago)

I would just re-pc and let them cool properly, there shouldn't be bubbles after that. I have done the same thing with some chunky oatmeal agar that was ridiculous. What I found was it worked but the new agar doesn't bond to the old agar and when you go to transfer you will notice the layers. I doubt it matters to the myc I just didn't like it.


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OfflineA.k.aM
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Re: Pouring new agar on top of unused agar? [Re: spiritlands]
    #26412258 - 01/02/20 10:23 AM (4 years, 1 month ago)

Seems like it’d work, I’m curious what black agar will look like, I can’t decide if it’ll make it easier or harder to see contams. For sure easier to see mycelium.

I have a handful of plates with bubbles too and it doesn’t seem to slow growth at all and actually looks kinda cool from the bottom sometimes.

The only potential problem is that it can look like there’s weird growth going on if you forget the bubbles are there, but soon as you check the back you see what it is.


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InvisibleSpunkyMonkey88
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Re: Pouring new agar on top of unused agar? [Re: A.k.a]
    #26412263 - 01/02/20 10:29 AM (4 years, 1 month ago)

Quote:

A.k.a said:
Seems like it’d work, I’m curious what black agar will look like, I can’t decide if it’ll make it easier or harder to see contams. For sure easier to see mycelium.

I have a handful of plates with bubbles too and it doesn’t seem to slow growth at all and actually looks kinda cool from the bottom sometimes.

The only potential problem is that it can look like there’s weird growth going on if you forget the bubbles are there, but soon as you check the back you see what it is.




Haha right!

As soon as I read dudes post I went in Amazon and ordered a bottle of black food coloring...

They say dog food agar is so nice because of how easy it is to see tams because of the contrast with it being so dark...


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OfflineFonzee
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Re: Pouring new agar on top of unused agar? [Re: SpunkyMonkey88]
    #26412313 - 01/02/20 10:54 AM (4 years, 1 month ago)

Black agar sounds nice, would love to see a picture of a plate once ready.

If your plates are clean and it seems you have a nice flow hood, just lift and discard the previous medium and pour again. Why bother keeping it?


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Offlinenaturalistic123
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Re: Pouring new agar on top of unused agar? [Re: Fonzee]
    #26412357 - 01/02/20 11:20 AM (4 years, 1 month ago)

I'd use the ones with the bubbles.  I've used all kinds of crazy looking agar dishes, including ones that were poured way too thick, or ones poured while the agar set up, with mountains and valleys in the dish.  I still use them and they all work fine. 

Then again Im a no waste kind of person, ocd type


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Ὡς οὖν εἴπεν αὐτοῖς ὅτι Ἐγώ εἰμι, ἀπῆλθον εἰς τὰ ὀπίσω, καὶ ἔπεσον χαμαί.


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North Spore Shop: Cultivation Supplies


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