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OfflineActionjunkie
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Isolation on agar assessment
    #26411960 - 01/02/20 06:41 AM (4 years, 27 days ago)

Been working on isolations. Do these look like successful isolations?


Now what do I do? Expand to more agar, then inoculate grain, or jar then g2g? Honestly I don't completely understand what I'm looking at. I just been watching vids and trying to follow directions. Any help, suggestions, links would be appreciated.



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OfflineThe Mycologist
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Re: Isolation on agar assessment [Re: Actionjunkie]
    #26411962 - 01/02/20 06:43 AM (4 years, 27 days ago)

Isolations of mycelium; yes


Monoculture; no


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That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
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OfflineActionjunkie
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Re: Isolation on agar assessment [Re: The Mycologist]
    #26411971 - 01/02/20 06:55 AM (4 years, 27 days ago)

How do I get to a monoculture? What's the benefits?


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OfflineThe Mycologist
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Re: Isolation on agar assessment [Re: Actionjunkie]
    #26411979 - 01/02/20 07:04 AM (4 years, 27 days ago)

You have to do 100s of transfers.

There isn't much benefit. You could do correct experiments because its only one genetic strain in there. But thats basically it.

Sometimes you can even find a monoculture that doesnt fruit lol

So whats best is to just ensure you got clean growth (poly culture I guess) and dont stress about a monoculture.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineActionjunkie
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Re: Isolation on agar assessment [Re: The Mycologist]
    #26411994 - 01/02/20 07:20 AM (4 years, 27 days ago)

So don't bother with isolating at all? Just keep spawn clean and fruit?

And thanks for the knowledge btw.


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OfflineThe Mycologist
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Re: Isolation on agar assessment [Re: Actionjunkie]
    #26412003 - 01/02/20 07:25 AM (4 years, 27 days ago)

Yea thats the consensus.

3-4 transfers to ensure its clean. Then to grain.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineActionjunkie
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Re: Isolation on agar assessment [Re: The Mycologist] * 1
    #26412034 - 01/02/20 07:51 AM (4 years, 27 days ago)

Awesome, thank you sir!


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