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OfflineDell500
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Cobweb or myc?
    #26411211 - 01/01/20 04:35 PM (4 years, 28 days ago)

Looks like both to me, but I'd love some other eyes on these cultures.

EQ on MAE (less nutritious recipe)

Aerial myc or cobweb? There's def myc in there, but I'm not sure if it's aerial or cobweb.

If it is cobweb, is it salvageable?


1

2

3

4

5

6


Edited by Dell500 (01/02/20 07:29 PM)


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OfflineDell500
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Re: Cobweb or myc? [Re: Dell500]
    #26412181 - 01/02/20 09:32 AM (4 years, 27 days ago)

Bumpity bump bump bump


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Offlinespiritlands
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Re: Cobweb or myc? [Re: Dell500]
    #26412241 - 01/02/20 10:11 AM (4 years, 27 days ago)

Can't zoom your pics, I don't see any cobweb but the myc does look strange. Cobweb is a fast mover in my book, when you get it you know. And it has a smell of it's own. I haven't had it in a long time so I can't describe the odor but you know it when it bites your nose.


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Germinating old spores progress page
how to calibrate your Martha



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OfflineDell500
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Re: Cobweb or myc? [Re: spiritlands]
    #26412312 - 01/02/20 10:54 AM (4 years, 27 days ago)

@spiritlands yea, the myc just looks odd tho. Really aerial like cobweb. I had to shrink the images down. I'll see if I can get a better closeup.


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Offlinefootpath
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Re: Cobweb or myc? [Re: Dell500]
    #26412395 - 01/02/20 11:45 AM (4 years, 27 days ago)

It is hard to tell from those pictures, but it is clearly fighting something.
As made evident by:
Plate 1, 3-4 o'clock
Plate 3, 4-6 o'clock
Plate 4 looks to be in quite a battle. If it was transferred at the same time as the others, I'd probably avoid taking further transfers from it.
Plate 5, 3 o'clock

Plate 6 looks pretty good, but you likely have something tangled in there yet.

How many transfers in are these?


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OfflineDell500
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Re: Cobweb or myc? [Re: footpath]
    #26412425 - 01/02/20 12:13 PM (4 years, 27 days ago)

2 from an agar pin that I thought was clean.

Here's a few more "close-ups". Hard to get it not to be blurry. Sorry.



This is one plate. But they all seem to have this same furriness.


Edited by Dell500 (01/02/20 07:26 PM)


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Offlinefootpath
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Re: Cobweb or myc? [Re: Dell500] * 1
    #26412460 - 01/02/20 12:32 PM (4 years, 27 days ago)

Hard to say if it's a mold mycelium or just tomentose cube mycelium growth, one culture can display both types.
But I'd still assume there's something in there since the culture isn't growing uniformly.

You might have better luck getting some identification if you take a picture of it backlit like so:

That way we might be able to see the stuff that might be growing around/under/beside it.

Uploading pictures to the site makes them expandable thumbnails like that one above.


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OfflineDell500
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Re: Cobweb or myc? [Re: footpath]
    #26413132 - 01/02/20 07:23 PM (4 years, 26 days ago)

This is the best I could do.



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Offlinefootpath
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Re: Cobweb or myc? [Re: Dell500]
    #26413174 - 01/02/20 07:55 PM (4 years, 26 days ago)

Ah, I can enlarge the others now, too. May have been something on my end, sorry if I came off a bit condescending.

I'm not 100%, but it does look that could be a contaminant tomentose mycelium.

I'd transfer them out again either way about it, it definitely looks like you have plenty of clean growth there to choose from.


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OfflineDell500
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Re: Cobweb or myc? [Re: footpath]
    #26413402 - 01/02/20 10:22 PM (4 years, 26 days ago)

Yea I'm gonna transfer a few more times to see if I can get a clean culture.

And no worries. You weren't condescending. I fixed the other links 😁


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OfflineMushr00mSurprise
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Registered: 12/12/19
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Re: Cobweb or myc? [Re: Dell500]
    #26413462 - 01/02/20 11:33 PM (4 years, 26 days ago)

Looks fine to me.


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OfflinePsychgro
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Re: Cobweb or myc? [Re: Mushr00mSurprise]
    #26424001 - 01/09/20 06:05 AM (4 years, 20 days ago)

Brand new newbie here...

After reading lots about tomentose vs Rizo here on the forum I understand that a culture coming from MS and cultures high in nutes can promote tomentose growth. I would imagine if your wedges came from different batches of agar the nutrients might be different causing tomentose growth on the transferred wedge then Rizo once on the new plate. I would imagine Rizo vs tomentose can also occur even if it came from the same batch of agar but different pours (due to concentration of nutes changing because of evaporation when reheating).

To my newb eyes your plates look good (just different sectors growing out at different rates) but I would do the same as you’re going to do and transfer again...maybe transferring onto a low nute agar can help.... I wonder if scraping (inoculation loop) an even smaller sample than a wedge would help


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Looking to learn all about it


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OfflineDell500
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Re: Cobweb or myc? [Re: Psychgro]
    #26424545 - 01/09/20 12:19 PM (4 years, 20 days ago)

I let the plates go and they all seem to be fine.

And I have the same hypothesis as you.  Possibly the nute content of the agar is making it grow vertically instead of horizontally.

All the plates I showed last week all seem to be fine.  They all grew out the edges and rhizo seems to be only centered around the transferred part.  I have other plates that definitely have cobweb in them as those plates have halted growth.

I'm making some jars up (rye and oats).  So we'll see how well these grow out and if they produce any cobweb.  Which is usually much easier to notice in grain than on agar, IMO.

I'll try to get some more pics up of the progress, but all seems ok so far.


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OfflineA.k.aM
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Re: Cobweb or myc? [Re: Dell500]
    #26424562 - 01/09/20 12:27 PM (4 years, 20 days ago)

They’re fine.

Some cool looking rhizo on there too.


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LAGM2020


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OfflineBLINKfan420
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Registered: 07/06/12
Posts: 1,078
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Re: Cobweb or myc? [Re: A.k.a]
    #26424574 - 01/09/20 12:32 PM (4 years, 20 days ago)

Its hard for me to see anything in the pics but I'm on a phone so maybe that has something to do with it.

When in doubt keep transferring. :shrug:

BLiNK


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All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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