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Offlinejfaustus
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Registered: 12/02/19
Posts: 11
Last seen: 1 year, 1 month
Contam? Help pls! White cottony fluff...
    #26409272 - 12/31/19 12:32 PM (4 years, 1 month ago)

Hi friends!  Progressing on my very first grow, but worried I might have some contamination.

Super happy so far, went with agar, got slightly less terrible at pouring plates, and worked on my sterile skills.  Can't say I'm good at any of it yet, but had a nearly 100% colonized plate. Very pretty.  Before dropping it in jars I wanted to do a few transfers.

Now at the site of the transfer cuts, it's kind gotten bushy/cottony white.  Dunno if that's a reaction to being cut, something to do with there being no agar to grow on, or if I managed to bring in a contaminant despite by best efforts.

First pic is slightly clearer.





Thanks in advance for the help!

-jfaustus


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OfflineRaccoon
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Registered: 04/08/19
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Re: Contam? Help pls! White cottony fluff... [Re: jfaustus]
    #26409300 - 12/31/19 12:45 PM (4 years, 1 month ago)

I wouldn't trust that dish in grain. The growth looks like cube myc, but that margin is a sign of a contam. The contaminate is blocking the myc from growing. As to what the contam is, I don't know. That fuzzy growth looks like cube mycelium to me, but it cant grow into that "corner" because of something. However, if you transfered from this dish at a point away from that spot, you're fine.

And what do you mean by the "site of the transfer cuts?"


--------------------
First Grow


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Offlinejfaustus
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Re: Contam? Help pls! White cottony fluff... [Re: Raccoon]
    #26409304 - 12/31/19 12:50 PM (4 years, 1 month ago)

Where it's fluffed out is where I cut pieces away to transfer to other plates. So it can't grow into that corner there because no agar.


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OfflineA.k.aM
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Re: Contam? Help pls! White cottony fluff... [Re: jfaustus]
    #26409348 - 12/31/19 01:20 PM (4 years, 1 month ago)

Yeah when it runs out of agar it can fluff up. I had a couple plates where I was at the end of the bottle and it only filled up like 80% of the plate. When it grew into the thinner/empty part it got extremely fluffy.


--------------------
LAGM2020


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OfflineRaccoon
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Registered: 04/08/19
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Re: Contam? Help pls! White cottony fluff... [Re: jfaustus]
    #26409562 - 12/31/19 03:47 PM (4 years, 1 month ago)

I think you are fine then! Looking good :smile:


--------------------
First Grow


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Offlinejfaustus
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Registered: 12/02/19
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Last seen: 1 year, 1 month
Re: Contam? Help pls! White cottony fluff... [Re: Raccoon]
    #26409719 - 12/31/19 06:12 PM (4 years, 1 month ago)

Thanks for the help, and Happy New Year to all!


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