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OfflineeLeSDenes
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Aspiration Jar for LC
    #26409172 - 12/31/19 11:14 AM (4 years, 29 days ago)

In the following post I am going to show you how I make my LC jars.
There are many teks exist today and most people are just pouring LC nowadays, but there are cases when you prefer to use syringes or inoculation guns (spawn bags).
Injection ports are obsolete and are always a contamination factor, but only they provide a possibility to 'cleanly' fill up your syringes.... Until now...

My solution is ------> Aspiration jars

You are going to need these :
4mm barbed bulkhead fittings


4mm silicon tubing


First you get your standard filtered lid (could be sfd or watman filters), then drill another hole to fit your bulkhead fitting. Use some silicon when you install it for sealing.
It will look like this:








Then, to cover the hole while it is colonising, I use a piece of silicon tubing with a blob of silicon in one end. I use this as a protecting cap to avoid contaminants falling in through the tube.




You also need to attach the silicon tubing to the bottom part of the bulkhead fitting


I pressure cook the liquids for 20min, then inoculate with a tiny wedge of agar when cooled down.
I like to wrap the port and the jar lid with parafilm for extra security.
When the LC is ready, you can take the silicon cap off and flame sterilise the port (it is made of brass and can be heated).
Then, you attach your sterile syringe with a sterile silicon tubing (shown in the next image) to the port and aspire the liquid.
When you fill up multiple syringes, just swap the syringe and leave the tubing on.
After you are finished, flame sterilise the port again and put your silicon port cap back on the port.

You can use this:


Or use a self refilling syringe or LC gun:


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InvisibleSpunkyMonkey88
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Re: Aspiration Jar for LC [Re: eLeSDenes]
    #26409200 - 12/31/19 11:40 AM (4 years, 29 days ago)

So you pull the plug so to speak, and heat the brass, where, in open air?

I think injection ports can a pretty safe means to do LC... just my Opinion tho

I'm considering just switching to Mason jars with a unmodified lid and drawing up the lc in a SAB...


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OfflineeLeSDenes
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26409208 - 12/31/19 11:47 AM (4 years, 29 days ago)

Of course not open air, in front of the flowhood or SAB but I thought I don't have to make it more obvious :laugh:

Injection ports are okay with small jars but you need a long ass needle for large jars or tip the jar but it is annoying as hell. With this mod I can fill up many syringes quickly and safely


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InvisibleSpunkyMonkey88
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Re: Aspiration Jar for LC [Re: eLeSDenes]
    #26409519 - 12/31/19 03:09 PM (4 years, 29 days ago)

Quote:

eLeSDenes said:
Of course not open air, in front of the flowhood or SAB but I thought I don't have to make it more obvious :laugh:

Injection ports are okay with small jars but you need a long ass needle for large jars or tip the jar but it is annoying as hell. With this mod I can fill up many syringes quickly and safely




I just tip my lc jars completely upside down...that's what whatmans are for yo


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Invisiblegizmo1
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26409599 - 12/31/19 04:33 PM (4 years, 29 days ago)

Quote:

SpunkyMonkey88 said:
Quote:

eLeSDenes said:
Of course not open air, in front of the flowhood or SAB but I thought I don't have to make it more obvious :laugh:

Injection ports are okay with small jars but you need a long ass needle for large jars or tip the jar but it is annoying as hell. With this mod I can fill up many syringes quickly and safely




I just tip my lc jars completely upside down...that's what whatmans are for yo



:eek: you my friend are a mad man. A complete mad man. I'm scared of lc swishing close to the edges of a lid and you are flipping these bitches upside down to draw into a syringe. I assume you use metal lids?


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Offlinespiritlands
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Re: Aspiration Jar for LC [Re: gizmo1]
    #26409624 - 12/31/19 04:58 PM (4 years, 29 days ago)

It's a good idea. It's basically how mine work but I use leurlock syringe tips as a tube I just cut the tip off. Works like a soda straw so I don't have to tip to fill syringes. I can  clean them and sterilize them while reusing the needle for each lc. The cap that comes with the needle tip works just like your cap. Then I use press and seal wrap to wrap up the seal of the lid and jar. I gather the loose ends of the wrap at the top to stop things from falling on my ge hole with mp tape. It can still breath but it's protected during colonization. I have magnetic stir bars in there for breaking up clumps. I love them. I don't need large quantities of lc but your setup looks convenient if I did. Good job.


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Edited by spiritlands (12/31/19 05:04 PM)


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InvisibleSpunkyMonkey88
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Re: Aspiration Jar for LC [Re: spiritlands]
    #26409673 - 12/31/19 05:31 PM (4 years, 29 days ago)

Ya dude I dont know that all looks like alot of work and just so much going on to be real effective against contamination that's just my opinion plz dont take offense man...

And to the other poster, ya I tip them bitches all day even shake em...the watman rarely even let's water or media through unless its pressurized but it lets air through when you draw liquid out through the injection port eliminating the possibility of a vacuum...no need for GE theres plenty of air in a half filled jar for the mycelium...


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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26409689 - 12/31/19 05:41 PM (4 years, 29 days ago)

Lol bro didn't you just have a thread about your whatmans leaking from shaking and something about breaking a jar from shaking with marbles?

It's not good practice to get your filters wet or the shake and flip LC while drawing into a syringe atleast thats how I see it especially if usimg plastic lids.

I wouldn't cut any sort of corners with lc it's just too easy to end up with a bunch of wasted supplies. Especially with a tested lc that's was previously clean then contaminated from shaking or flipping.

I'm not trying to be a buzzkill or anything just maybe not the best advice to give to others even if it works great for you.


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Offlinespiritlands
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26409714 - 12/31/19 06:08 PM (4 years, 29 days ago)

No offense taken. They're just something I'm trying bc I've decided to relearn lc's. I've been using them for two months, just something I saw on YouTube with my own twist. I've made about 8 lc's with these so far. One was a dud nothing grew, the rest all did great. The only one I had go bad was one I used to fill syringes and then came back to it a couple weeks later. I like wrapping my jars bc I seal it while it's in the flow Hood and I don't unseal it till it's back in the flow Hood. Call it paranoia but I'ma keep doing it, it's been working for me. I like your idea I would just have to step up my game to need that much lc ;-}>


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InvisibleSpunkyMonkey88
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Re: Aspiration Jar for LC [Re: gizmo1]
    #26409737 - 12/31/19 06:26 PM (4 years, 29 days ago)

Quote:

gizmo1 said:
Lol bro didn't you just have a thread about your whatmans leaking from shaking and something about breaking a jar from shaking with marbles?

It's not good practice to get your filters wet or the shake and flip LC while drawing into a syringe atleast thats how I see it especially if usimg plastic lids.

I wouldn't cut any sort of corners with lc it's just too easy to end up with a bunch of wasted supplies. Especially with a tested lc that's was previously clean then contaminated from shaking or flipping.

I'm not trying to be a buzzkill or anything just maybe not the best advice to give to others even if it works great for you.




Ya I did but after making that post I've come to the conclusion that even a wet whatman doesn't pose any threat to contamination they are literally made to filter bacteria out liquids...

Lots of people use the same setup including the guys at mushroom revival...a commercial mushroom supplier dealing with gourmet and medicinal mushrooms...

And ya I did break a few jars cause I went a little nuts shaking them, but I was just making a point of how effective the whatmans are compared to a SFD...


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Re: Aspiration Jar for LC [Re: spiritlands]
    #26409746 - 12/31/19 06:31 PM (4 years, 29 days ago)

Quote:

spiritlands said:
No offense taken. They're just something I'm trying bc I've decided to relearn lc's. I've been using them for two months, just something I saw on YouTube with my own twist. I've made about 8 lc's with these so far. One was a dud nothing grew, the rest all did great. The only one I had go bad was one I used to fill syringes and then came back to it a couple weeks later. I like wrapping my jars bc I seal it while it's in the flow Hood and I don't unseal it till it's back in the flow Hood. Call it paranoia but I'ma keep doing it, it's been working for me. I like your idea I would just have to step up my game to need that much lc ;-}>




Ya dude I gotcha and different strokes for different folks... if it works for u go for it...

I'm honestly I'm evolving and am super close to just being done with modded lc jars, period and just keeping g it super simple...

I'm thinking just a wide mouth pint with a plastic lid with a gasket. Just draw up your liquid in the SAB...no filters no ports no problems...

Only thing is youd pretty much have to put your hand over the jar to draw up the liquid which could ruin everything....

What do you think?


Edited by SpunkyMonkey88 (12/31/19 06:34 PM)


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OfflineeLeSDenes
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26409759 - 12/31/19 06:37 PM (4 years, 29 days ago)

Problem with getting the watman filter wet with LC is that the mushroom will colonise it and won't let air through. Maybe it is not a huge problem with small LC jars but it is with half gallon sized ones.


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InvisibleSpunkyMonkey88
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Re: Aspiration Jar for LC [Re: eLeSDenes]
    #26409761 - 12/31/19 06:39 PM (4 years, 29 days ago)

Quote:

eLeSDenes said:
Problem with getting the watman filter wet with LC is that the mushroom will colonise it and won't let air through. Maybe it is not a huge problem with small LC jars but it is with half gallon sized ones.




Dam dude... I never thought about that...

That could be a problem cus that vacuum will want to suck air in where ever it can even holes in the injection ports...


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Invisiblegizmo1
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26409777 - 12/31/19 06:51 PM (4 years, 28 days ago)

Quote:

SpunkyMonkey88 said:
Quote:

gizmo1 said:
Lol bro didn't you just have a thread about your whatmans leaking from shaking and something about breaking a jar from shaking with marbles?

It's not good practice to get your filters wet or the shake and flip LC while drawing into a syringe atleast thats how I see it especially if usimg plastic lids.

I wouldn't cut any sort of corners with lc it's just too easy to end up with a bunch of wasted supplies. Especially with a tested lc that's was previously clean then contaminated from shaking or flipping.

I'm not trying to be a buzzkill or anything just maybe not the best advice to give to others even if it works great for you.




Ya I did but after making that post I've come to the conclusion that even a wet whatman doesn't pose any threat to contamination they are literally made to filter bacteria out liquids...

Lots of people use the same setup including the guys at mushroom revival...a commercial mushroom supplier dealing with gourmet and medicinal mushrooms...

And ya I did break a few jars cause I went a little nuts shaking them, but I was just making a point of how effective the whatmans are compared to a SFD...



I have nothing against whatmans we have been using them for years. Just bad practice to flip a lc jar. Then some new grower may read this and think it's ok to do the same even though he/she doesnt have any gaskets in their lids effectively washing contams down into the jar and wondering what went wrong because SpunkeyMonkey said it was ok to do.
Most people dont use those gaskets or leak proof lids just plastic lids.
In these instances the jar should not be turned upside down.
I'm just trying to help here though like I said I'm not trying to hate or anything just maybe it's not the best advice to give is my only point. Maybe instead giving the details like lids with leak proof lids with whatmans can be flipped to draw lc into syringe or something along those lines. I think even then I wouldn't flip my lc but remember most people who read our advice will be new growers who dont know that regular plastic lids will lead everywhere and whatnot.
Either way just saying hope everyone has a happy new years. The beers are ice cold on this end.  :rockon:


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Invisiblegizmo1
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Re: Aspiration Jar for LC [Re: eLeSDenes]
    #26409779 - 12/31/19 06:53 PM (4 years, 28 days ago)

Quote:

eLeSDenes said:
Problem with getting the watman filter wet with LC is that the mushroom will colonise it and won't let air through. Maybe it is not a huge problem with small LC jars but it is with half gallon sized ones.



Is that what happens? I always though my knockoff whatmans just sucked ass and weren't good for more than one or two uses. They would always get plugged somehow and I never knew what was going on.


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Re: Aspiration Jar for LC [Re: gizmo1]
    #26409788 - 12/31/19 07:00 PM (4 years, 28 days ago)

🤔🍿


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Offlinespiritlands
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26409803 - 12/31/19 07:18 PM (4 years, 28 days ago)

Quote:

SpunkyMonkey88 said:
Quote:

spiritlands said:
No offense taken. They're just something I'm trying bc I've decided to relearn lc's. I've been using them for two months, just something I saw on YouTube with my own twist. I've made about 8 lc's with these so far. One was a dud nothing grew, the rest all did great. The only one I had go bad was one I used to fill syringes and then came back to it a couple weeks later. I like wrapping my jars bc I seal it while it's in the flow Hood and I don't unseal it till it's back in the flow Hood. Call it paranoia but I'ma keep doing it, it's been working for me. I like your idea I would just have to step up my game to need that much lc ;-}>




Ya dude I gotcha and different strokes for different folks... if it works for u go for it...

I'm honestly I'm evolving and am super close to just being done with modded lc jars, period and just keeping g it super simple...

I'm thinking just a wide mouth pint with a plastic lid with a gasket. Just draw up your liquid in the SAB...no filters no ports no problems...

Only thing is youd pretty much have to put your hand over the jar to draw up the liquid which could ruin everything....

What do you think?



I've been using glass tea bottles from the gas station. I do have a hole in top with mp tape and I do my wrap-it-up thing. Been trying to just pour it but I don't have a feel for how much I pour yet. Tried it on some cakes they're doing well but I had a couple get trich. I'm using them to do the slurry in a hurry. I thinks it's changed my life. I turned a pint cake into 3 gallons almost colonized and it's only been three days. I had extra so I just spawned the slurry to substrate and skipped going to grain. So between the lc's and the blender tek I save about three weeks. I haven't done it yet but it's happening right now and I'm impressed.


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InvisiblemushboyMDiscord
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Re: Aspiration Jar for LC [Re: spiritlands]
    #26409827 - 12/31/19 07:41 PM (4 years, 28 days ago)

using media bottles that have those no drip dealies really helps wwith pouring lc:thumbup:


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Re: Aspiration Jar for LC [Re: mushboy]
    #26409872 - 12/31/19 08:15 PM (4 years, 28 days ago)

That's a great idea, thanks.
Like what's on a liquor bottle at a bar? Right?


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Re: Aspiration Jar for LC [Re: spiritlands]
    #26409912 - 12/31/19 08:38 PM (4 years, 28 days ago)

Quote:

spiritlands said:
That's a great idea, thanks.
Like what's on a liquor bottle at a bar? Right?



Na bro like a pyrex media bottle they have a little no drip ring on them. You can get the budget ass ones on amazon pretty cheap. I like them.


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Re: Aspiration Jar for LC [Re: gizmo1]
    #26409937 - 12/31/19 09:01 PM (4 years, 28 days ago)

Ha ok o check it out. I'm loving these glass pitris.


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InvisiblemushboyMDiscord
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Re: Aspiration Jar for LC [Re: spiritlands]
    #26409953 - 12/31/19 09:14 PM (4 years, 28 days ago)


the little blue dealie


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InvisibleSpunkyMonkey88
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Re: Aspiration Jar for LC [Re: mushboy]
    #26410402 - 01/01/20 03:37 AM (4 years, 28 days ago)

Quote:

mushboy said:
using media bottles that have those no drip dealies really helps wwith pouring lc:thumbup:




Thata not a bad idea but that also makes for some expensive lcs...canning jar are like 25 cents a peice...

What Bout drawing up the lc theough a sterile syringe from an open unmodified jar in a SAB, does that sound sketchy?


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OfflineeLeSDenes
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26410563 - 01/01/20 08:02 AM (4 years, 28 days ago)

pouring LC with media bottles is the shit if you have jars to inoculate, the pouring ring makes it so much easier. With bags I much prefer shooting the LC through a syringe.

You can do unmodified if that is your thing, it is not sketchy at all if you have good hands, but I like to shorten processes and make it more efficient for bulk.
My idea behind the aspiration jar was that you can just quickly swap the syringes on the silicon  tube and flame sterilise the port, so you can fill up as many syringes as you want with minimal exposure. This operation only exposes a tiny fraction of the sterile surface (the port and the end of the silicon tube while you swap syringes) whereas opening the unmodified lid could possibly let shit from my hands fall into the jar.
I have a flowhood, but I take even more precautions that SAB people do :laugh:


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