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Offlinespiritlands
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Registered: 06/21/10
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Re: Aspiration Jar for LC [Re: gizmo1]
    #26409937 - 12/31/19 09:01 PM (4 years, 29 days ago)

Ha ok o check it out. I'm loving these glass pitris.


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Germinating old spores progress page
how to calibrate your Martha



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InvisiblemushboyMDiscord
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Re: Aspiration Jar for LC [Re: spiritlands]
    #26409953 - 12/31/19 09:14 PM (4 years, 29 days ago)


the little blue dealie


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InvisibleSpunkyMonkey88
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Registered: 10/08/19
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Re: Aspiration Jar for LC [Re: mushboy]
    #26410402 - 01/01/20 03:37 AM (4 years, 28 days ago)

Quote:

mushboy said:
using media bottles that have those no drip dealies really helps wwith pouring lc:thumbup:




Thata not a bad idea but that also makes for some expensive lcs...canning jar are like 25 cents a peice...

What Bout drawing up the lc theough a sterile syringe from an open unmodified jar in a SAB, does that sound sketchy?


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OfflineeLeSDenes
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Re: Aspiration Jar for LC [Re: SpunkyMonkey88]
    #26410563 - 01/01/20 08:02 AM (4 years, 28 days ago)

pouring LC with media bottles is the shit if you have jars to inoculate, the pouring ring makes it so much easier. With bags I much prefer shooting the LC through a syringe.

You can do unmodified if that is your thing, it is not sketchy at all if you have good hands, but I like to shorten processes and make it more efficient for bulk.
My idea behind the aspiration jar was that you can just quickly swap the syringes on the silicon  tube and flame sterilise the port, so you can fill up as many syringes as you want with minimal exposure. This operation only exposes a tiny fraction of the sterile surface (the port and the end of the silicon tube while you swap syringes) whereas opening the unmodified lid could possibly let shit from my hands fall into the jar.
I have a flowhood, but I take even more precautions that SAB people do :laugh:


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