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Offlinefeldman114
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Help me salvage this epic fail of a PE grow
    #26408915 - 12/31/19 08:01 AM (4 years, 29 days ago)

Original PA has kicked my ass. I can’t get anything but super dense blobs, and even 100% BE is barely achieved.
But I still have 5 mini-tubs and a big mono that are all either pinning or about 95% colonized, so I figured maybe you guys can help me pull some actual mushrooms off them.

Ok, so these were all MS>g2g>coir except the big tub which was agar>master>g2g>coir, so let’s start there. Spawn was beyond-a-doubt clean and I cased (with coir only) right at 100% colonization. Instead of pinning, it started eating up the casing. Now, after another week, I got like 4 blobs in the entire tub.
Another dumb thing I did was experiment with tub configuration. The lid doesn’t seal at all, so I skipped the top holes, and made “slits” instead of holes on the bottom. The idea was to be able to remove the mp tape completely after the pinset comes in...




So far, the minis “pin” blobs all over, on every flush, like so.



I had 2 more mini’s but lost them to lipstick mold after flush#3.



Any and all suggestions will be appreciated


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InvisibleSpunkyMonkey88
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26408949 - 12/31/19 08:38 AM (4 years, 29 days ago)

I've only ever had one tray blob out on me and that was because (I think so anyway) of not enough FAE and bacteria overgrowth...

How are you so certain your spawn is clean?


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: SpunkyMonkey88]
    #26408956 - 12/31/19 08:46 AM (4 years, 29 days ago)

I’m only sure about the big tub. I cleaned it on agar, made a beautiful-looking master from the 5th transfer and g2g’d. Only used jars I was 100% sure about, but still gave them a “control shake” at 100% - they recovered fully in 2 days,

You might be up to something with FAE. I’m gonna take all the mp off of one of the cased minis, see how it turns out. Also gonna add more pics after werk.

Here’s my weird hole slit placement. 5 1/2-in slits next to each other...idk what I was thinking.


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OfflineMycoStu
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26408968 - 12/31/19 08:53 AM (4 years, 29 days ago)

""Mutant" Blobs - Blobs usually appear when pinning first begin with less blobs being produced later. Mutant blobs are the result of genetic abnormalities and are particularly common in degenerated cultures and certain varieties such as Penis Envy. Environmental conditions also seem to play a role in blob development. On PF cakes they are mostly seen in when cakes are fruited immediately after full colonization."

Copy and paste from another post.
Good luck! :cheers:


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: MycoStu]
    #26408983 - 12/31/19 09:01 AM (4 years, 29 days ago)

Quote:

MycoStu said:
""Mutant" Blobs - Blobs usually appear when pinning first begin with less blobs being produced later. Mutant blobs are the result of genetic abnormalities and are particularly common in degenerated cultures and certain varieties such as Penis Envy. Environmental conditions also seem to play a role in blob development. On PF cakes they are mostly seen in when cakes are fruited immediately after full colonization."

Copy and paste from another post.
Good luck! :cheers:



Thanks mayne!

But this isn’t a 1st flush problem. I got exactly 1 mushroom in 3 flushes, from 2 tubs. 3oz dry of blobs and ONE fuckin mushroom.
The freakiest part of the blobs is how dense they are. An oz of them has the volume of 3.5g of regular shrooms. Potent little fuckers too - took 30g fresh and it felt like a 70g trip.


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InvisibleSpunkyMonkey88
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26409006 - 12/31/19 09:18 AM (4 years, 29 days ago)

Quote:

feldman114 said:
I’m only sure about the big tub. I cleaned it on agar, made a beautiful-looking master from the 5th transfer and g2g’d. Only used jars I was 100% sure about, but still gave them a “control shake” at 100% - they recovered fully in 2 days,

You might be up to something with FAE. I’m gonna take all the mp off of one of the cased minis, see how it turns out. Also gonna add more pics after werk.

Here’s my weird hole slit placement. 5 1/2-in slits next to each other...idk what I was thinking.





My blobs were from the malabar variety they were bacterial AF also and yellow. Most blobs turned into (mutated) mushrooms once I upped the FAE and quit misting... dont know if that's a coincidence or if that's what they were gonna do either way... but my tub was definitely not getting enough FAE...I too messed with hole placement and size when I should of just followed a tek...

Imo your tub does look pretty soggy...

Here's my malabars:

This is later on after upping the FAE when they started to form into mutants


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: SpunkyMonkey88]
    #26409028 - 12/31/19 09:35 AM (4 years, 29 days ago)

Yeah I made the casing on the wet side cause it’s a huge sub at 1:1 ratio, so I figured it can use the extra moisture. Idk, the myc peaking out looks pretty good, beaded surface and all.

I’m gonna try the extra FAE with one mini first. Could be the answer, just seems too easy to be true I guess


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26409040 - 12/31/19 09:40 AM (4 years, 29 days ago)

Btw, I have no issues fruiting normal cubes and getting around 200% BE in monos. This PE kicked my ass though.
I was feeling my myco skills before this lol. Now I remember the truth - I barely have ANY skills🤷🏻‍♂️


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InvisibleSpunkyMonkey88
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26409044 - 12/31/19 09:45 AM (4 years, 29 days ago)

Quote:

feldman114 said:
Yeah I made the casing on the wet side cause it’s a huge sub at 1:1 ratio, so I figured it can use the extra moisture. Idk, the myc peaking out looks pretty good, beaded surface and all.

I’m gonna try the extra FAE with one mini first. Could be the answer, just seems too easy to be true I guess




Ya dude water will bead and shit but if it's not evaporating at all IME then its prob not getting enough FAE.

My tub had those perfect water beads on the surface too... practically the while time and I barely misted cus there they were those perfect little water beads (just like that surface conditions thread says you want)

But then they pinned and then bam I got that nasty ass tray...


I think the beads of water are good but I dont think u want them on the surface ALL the time with out any evaporation...

But if you get 200%BE fuck man dont listen to me... I get like 45 gs per quart if I'm lucky...


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InvisibleSpunkyMonkey88
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Re: Help me salvage this epic fail of a PE grow [Re: SpunkyMonkey88]
    #26409046 - 12/31/19 09:45 AM (4 years, 29 days ago)

Sorry that was 45gs per 2 quarts... that's my best so far

That's off first flush only tho


Edited by SpunkyMonkey88 (12/31/19 09:46 AM)


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: SpunkyMonkey88]
    #26409053 - 12/31/19 09:52 AM (4 years, 29 days ago)

Quote:

SpunkyMonkey88 said:
Sorry that was 45gs per 2 quarts... that's my best so far

That's off first flush only tho



Wait, you’re not counting coir, right?

This may not be true for all reg varieties, but GT has always produced a 1.5-2.5oz per myco-quart of spawn. Then again, I worked with GT exclusively for years so I’m prolly biased.
I like to keep tubs around for 4-5 flushes though (except these damn PEs that contaminated after 3).


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InvisibleSpunkyMonkey88
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26409086 - 12/31/19 10:12 AM (4 years, 29 days ago)

Quote:

feldman114 said:
Quote:

SpunkyMonkey88 said:
Sorry that was 45gs per 2 quarts... that's my best so far

That's off first flush only tho



Wait, you’re not counting coir, right?

This may not be true for all reg varieties, but GT has always produced a 1.5-2.5oz per myco-quart of spawn. Then again, I worked with GT exclusively for years so I’m prolly biased.
I like to keep tubs around for 4-5 flushes though (except these damn PEs that contaminated after 3).





Ya that's per myco quart not including any coir or anything just grains..I think getting 1 dry OZ per 1 myco QT is approximately 100% BE so I'm just under that...

But yeah I usually throw my shit out after the first flush. Just don't feel like fucking with it anymore and I usually got colonized spawn waiting for an empty tub...


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: SpunkyMonkey88]
    #26436357 - 01/16/20 08:39 AM (4 years, 13 days ago)

Another mini-tub doing the saaaame shit. This one has a super thin casing and 1:1 spawn ratio.

Posting cause it is the first one to contam before the 4th flush. Lipstick mold as usual (for my appt). My question is - could it have been in all my tubs, making them blob out like that? Idk if it’s actual Lipstick mold or some other pink mold, but I now know it’s indistinguishable from myc until it sporulates. The pink appeared overnight.



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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26436379 - 01/16/20 08:50 AM (4 years, 13 days ago)

Closeups


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InvisibleStipe-n CapMDiscord
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26436385 - 01/16/20 08:54 AM (4 years, 13 days ago)


Quote:

Ya that's per myco quart not including any coir or anything just grains..I think getting 1 dry OZ per 1 myco QT is approximately 100% BE so I'm just under that...




100% biological efficiency occurs when 1 lb of fresh mushrooms is harvested from 1 lb of dry substrate, over multiple flushes.

So obviously if you use a 650 gr brick of coir, 650 grams of mush will need to be harvested to get 100% BE.

I consolidate my PE jars for 1 to 2 weeks prior to spawn. Try going 1:2 next time and using a "proper casing" with Peat/Verm 1:1, Hydrated lime 1 tsp per cup of dry peat.

I pretty much follow what LotKid does, it works like a charm. I also consolidate spawn for saprophytes.


Edited by Stipe-n Cap (01/16/20 09:01 AM)


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: Stipe-n Cap]
    #26436394 - 01/16/20 08:59 AM (4 years, 13 days ago)

Quote:

p9hu7 said:
100% biological efficiency occurs when 1 lb of fresh mushrooms is harvested from 1 lb of dry substrate, over multiple flushes.

I consolidate my PE jars for 1 to 2 weeks prior to spawn. Try going 1:2 next time and using a "proper casing" with Peat/Verm 1:1, Hydrated lime 1 tsp per cup of dry peat.

I pretty much follow what LotKid does, it works like a charm. I also consolidate spawn for saprophytes.




Very interesting. Had to google what saprophytes are lol.

But I tried a bunch of different spawn ratios and casing types, and ended up with the same results. I even layered one tub cause of something I read in a 10-year-old thread lol. Tried everything..,


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InvisibleStipe-n CapMDiscord
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26436399 - 01/16/20 09:03 AM (4 years, 13 days ago)

Have you tried consolidating your spawn?

Quote:


I had 2 more mini’s but lost them to lipstick mold after flush#3





This is an indication that your spawn isn't 100%. You should be able to flush until spent without contamination with clean spawn.

Next round of jars be extra super present and careful with sterile technique, consolidate spawn for 1+ week(s)
let tub fully colonize, then case with peat/verm and hydrated lime.

This should fix your tubs. If not then you have to dial in your tub

Side note*

I have a shoebox on flush 5 right now just  for shits, I want to see how far it can go...no contamination no side or bottom pins.


Edited by Stipe-n Cap (01/16/20 09:23 AM)


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OfflineA.k.aM
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Re: Help me salvage this epic fail of a PE grow [Re: Stipe-n Cap]
    #26436930 - 01/16/20 02:41 PM (4 years, 13 days ago)

I’ve only run PE once so idk what part made a difference but my tubs turned out great.

I basically did the same thing, syringe to wbs to coir, then cased at 90-100%.


The only thing I can think of I did different was once it hit 100 I let it sit and consolidate for another 3-5 days before fruiting.

I just reread I missed you cased with coir. That’s probably it, I ran two shoeboxes with 5050mix casing and one with coir, cased tube were both 320+ no blobs first flush and the coir tub was a handful of blobs.


--------------------
LAGM2020


Edited by A.k.a (01/16/20 02:44 PM)


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Offlinefeldman114
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Re: Help me salvage this epic fail of a PE grow [Re: A.k.a]
    #26436937 - 01/16/20 02:45 PM (4 years, 13 days ago)

Huh, I guess it’s a common theme.
I believe a few jars DID sit for a week, until a tub freed up. But fuck me if I can remember which one:sad:

Well, got 4 left. If consolidation is the culprit, I should get mushrooms from at least 1 of them.


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OfflineA.k.aM
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Re: Help me salvage this epic fail of a PE grow [Re: feldman114]
    #26436948 - 01/16/20 02:53 PM (4 years, 13 days ago)

I spawned the jars right at 100 but once it colonized the coir let it sit for a while before fruiting. Never tried letting spawn sit.

Casing was huge too.

The coir one was so bad I tried late casing which didn’t matter lol but this is what it did.

I put a peat casing on I think the day before the picture.
Vs both peat cased tubs did this


--------------------
LAGM2020


Edited by A.k.a (01/16/20 02:55 PM)


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