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Offlineroxxor
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Registered: 08/29/12
Posts: 251
Last seen: 7 months, 8 days
LC growth stalled
    #26408851 - 12/31/19 07:05 AM (4 years, 29 days ago)

2 or 3 weeks into incubation with a jar of LC. Looks good but growth has been the same for about a week now. Do I just have to try a syringe full and see? Think I did 4 tbsp of karo in 2/3 jar of water for the lc.




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InvisibleSpunkyMonkey88
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Registered: 10/08/19
Posts: 1,331
Re: LC growth stalled [Re: roxxor]
    #26408918 - 12/31/19 08:05 AM (4 years, 29 days ago)

Looks weird imo... should have more growth then that... did u use agar to inoculate it?


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OfflineSFS96
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Registered: 12/09/18
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Re: LC growth stalled [Re: SpunkyMonkey88]
    #26408919 - 12/31/19 08:10 AM (4 years, 29 days ago)

Flush that down the drain. I’m not sure if that’s even mushroom mycelium.


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: LC growth stalled [Re: roxxor]
    #26408937 - 12/31/19 08:29 AM (4 years, 29 days ago)

I feel like i had an issue before like that with Karo. I have noticed that filtered honey seems to promote better growth


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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InvisibleSpunkyMonkey88
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Registered: 10/08/19
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Re: LC growth stalled [Re: Diego]
    #26408945 - 12/31/19 08:35 AM (4 years, 29 days ago)

Quote:

Diego said:
I feel like i had an issue before like that with Karo. I have noticed that filtered honey seems to promote better growth




If that's karo that's carmelized, I thought it was a LME lc


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: LC growth stalled [Re: SpunkyMonkey88]
    #26408970 - 12/31/19 08:53 AM (4 years, 29 days ago)

Quote:

SpunkyMonkey88 said:
Quote:

Diego said:
I feel like i had an issue before like that with Karo. I have noticed that filtered honey seems to promote better growth




If that's karo that's carmelized, I thought it was a LME lc




Yeah probably got pc'd too long. He said Karo. I use filtered potato water and honey. Its made a huge difference imo


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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InvisibleSpunkyMonkey88
Stranger
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Registered: 10/08/19
Posts: 1,331
Re: LC growth stalled [Re: Diego]
    #26409016 - 12/31/19 09:23 AM (4 years, 29 days ago)

Quote:

Diego said:
Quote:

SpunkyMonkey88 said:
Quote:

Diego said:
I feel like i had an issue before like that with Karo. I have noticed that filtered honey seems to promote better growth




If that's karo that's carmelized, I thought it was a LME lc




Yeah probably got pc'd too long. He said Karo. I use filtered potato water and honey. Its made a huge difference imo




Oh ok also I just reread his post and noticed he put 4 tbsp in that jar (looks like a pint)... if that wasn't a typo dude put WAY too much karo in there.

When I used karo I did one tbsp per 500ML

Make the switch to LME


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: LC growth stalled [Re: SpunkyMonkey88]
    #26409026 - 12/31/19 09:32 AM (4 years, 29 days ago)

Quote:

SpunkyMonkey88 said:
Quote:

Diego said:
Quote:

SpunkyMonkey88 said:
Quote:

Diego said:
I feel like i had an issue before like that with Karo. I have noticed that filtered honey seems to promote better growth




If that's karo that's carmelized, I thought it was a LME lc




Yeah probably got pc'd too long. He said Karo. I use filtered potato water and honey. Its made a huge difference imo




Oh ok also I just reread his post and noticed he put 4 tbsp in that jar (looks like a pint)... if that wasn't a typo dude put WAY too much karo in there.

When I used karo I did one tbsp per 500ML

Make the switch to LME




Do you have a good tek you're willing to share for the lme? Im guessing thats light malt extract or am I incorrect?


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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InvisibleDigit
Surreal Estate Agent
Registered: 08/10/15
Posts: 69
Loc: tierra incognita
Re: LC growth stalled [Re: Diego]
    #26409956 - 12/31/19 09:16 PM (4 years, 29 days ago)

I used to do Karo LC's years ago and I always had really slow growth.
I just started up again after a long break with LME, as in light malt extract.
I may have cooked it too long and used to much LME, 2 grams/100ml, 30 min at 15lbs, but then I filtered out the sediment and cooked it again for 15 min, which is totally the wrong thing to do.
But it's colonizing super fast anyway and it looks really healthy.
Karo is mainly fructose and sucrose, which caramelize more easily than maltose, which is the main sugar in malt extract. So it's harder to overcook malt extract than corn syrup.
My point is that LME makes a way better LC than Karo for many different reasons.
I'd try starting over with LME if I were you, but only use .5 to 1 gram or less per 100ml. Some ppl only use 1 gram per 600ml. Using less LME in the broth gives you a clearer broth with less sediment. Cook it at 15lbs for 15 to 20 minutes. Use the best and most aggressive bit of agar on the plate, and use a wedge about 1/16th of the plate.

Happy New Year


--------------------
Question everything.
Believe nothing.
Think for yours3lf.


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InvisibleDigit
Surreal Estate Agent
Registered: 08/10/15
Posts: 69
Loc: tierra incognita
Re: LC growth stalled [Re: Digit]
    #26409965 - 12/31/19 09:19 PM (4 years, 29 days ago)

Oh yeah also get or make a magnetic stirrer. It makes a massive difference in the colonization time.


--------------------
Question everything.
Believe nothing.
Think for yours3lf.


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OfflineDr.Dank
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Registered: 02/03/12
Posts: 1,018
Loc: k Jaw
Last seen: 8 months, 3 days
Re: LC growth stalled [Re: Digit]
    #26410193 - 12/31/19 11:35 PM (4 years, 28 days ago)

No joke, ditch LCs. Grab Yourself some agar and make a few plates and master jars. That's it. Done deal, the most clean myc you'll ever have in abundance. Over thinking makes everything so difficult, it's extremely basic. PP5 dollar store containers work surprisingly well as Petri dishes if money is an issue.


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OfflineDr.Dank
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Re: LC growth stalled [Re: Dr.Dank]
    #26410209 - 12/31/19 11:49 PM (4 years, 28 days ago)

Even works haphazardly.


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: LC growth stalled [Re: Dr.Dank]
    #26410451 - 01/01/20 05:11 AM (4 years, 28 days ago)

Quote:

Canna-bliss1 said:
No joke, ditch LCs. Grab Yourself some agar and make a few plates and master jars. That's it. Done deal, the most clean myc you'll ever have in abundance. Over thinking makes everything so difficult, it's extremely basic. PP5 dollar store containers work surprisingly well as Petri dishes if money is an issue.




Alot of people won't buy a plate or spawn jar over a syringe for the most part. LCs are still important


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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InvisibleSpunkyMonkey88
Stranger
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Registered: 10/08/19
Posts: 1,331
Re: LC growth stalled [Re: Dr.Dank]
    #26410464 - 01/01/20 05:29 AM (4 years, 28 days ago)

Quote:

Canna-bliss1 said:
No joke, ditch LCs. Grab Yourself some agar and make a few plates and master jars. That's it. Done deal, the most clean myc you'll ever have in abundance. Over thinking makes everything so difficult, it's extremely basic. PP5 dollar store containers work surprisingly well as Petri dishes if money is an issue.




Ya dude I'm pretty sure most of us are using agar AND lcs not one or the other...

Its foolish to be so concerned about keeping your LC sterile then taking a dirty fucking syringe and shooting that shit in there....

Lcs are shit if you aren't using agar to knock them up, just a cesspool of contaminates breeding alongside your spores.

Lcs are fucking awesome if you inoculate with agar. Its basically a fluid version of agar that can be used to coat your grains with clean mycelium...

Dont hate


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OfflineDr.Dank
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Re: LC growth stalled [Re: SpunkyMonkey88]
    #26410735 - 01/01/20 10:50 AM (4 years, 28 days ago)

Lmao! Why doe's everybody on Shroomery think an opinion is hating on another? No hate in me, I've been lurking since 2012, I don't post a ton, but I research and read like crazy. I am simply stating that if you always keep a clean master jar to do g2g you essentially have unlimited mycelium. 1 jar to 6, 6 jars to 36, 36 jars to 216 etc, ya get the point. Yes Liquid Cultures have their place in mycology for sure, but it's silly to see people struggle with contams, over hydration etc, when it can be so so so simple. Much love though, and don't always jump to negative conclusions, it spreads negative energy:hug:


Edited by Dr.Dank (01/01/20 10:54 AM)


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InvisibleSpunkyMonkey88
Stranger
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Registered: 10/08/19
Posts: 1,331
Re: LC growth stalled [Re: Dr.Dank]
    #26410855 - 01/01/20 12:26 PM (4 years, 28 days ago)

Quote:

Canna-bliss1 said:
Lmao! Why doe's everybody on Shroomery think an opinion is hating on another? No hate in me, I've been lurking since 2012, I don't post a ton, but I research and read like crazy. I am simply stating that if you always keep a clean master jar to do g2g you essentially have unlimited mycelium. 1 jar to 6, 6 jars to 36, 36 jars to 216 etc, ya get the point. Yes Liquid Cultures have their place in mycology for sure, but it's silly to see people struggle with contams, over hydration etc, when it can be so so so simple. Much love though, and don't always jump to negative conclusions, it spreads negative energy:hug:



Quote:

Canna-bliss1 said:
Lmao! Why doe's everybody on Shroomery think an opinion is hating on another? No hate in me, I've been lurking since 2012, I don't post a ton, but I research and read like crazy. I am simply stating that if you always keep a clean master jar to do g2g you essentially have unlimited mycelium. 1 jar to 6, 6 jars to 36, 36 jars to 216 etc, ya get the point. Yes Liquid Cultures have their place in mycology for sure, but it's silly to see people struggle with contams, over hydration etc, when it can be so so so simple. Much love though, and don't always jump to negative conclusions, it spreads negative energy:hug:




Dude you took that statement to heart way more than you should of...

I say "dont hate" and all kinds of other goofy shit all the time, and dont mean much by it... I really am sorry I offended you or whatever but that wasn't my intention, seriously man.

Chill bro we are good, no negative vibes intended mayne...


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InvisibleDiego


Registered: 12/30/19
Posts: 346
Re: LC growth stalled [Re: Dr.Dank]
    #26410882 - 01/01/20 12:44 PM (4 years, 28 days ago)

Id just take those wedges and throw them in some LC flasks. Put them on an occilator and have LC syringes for days. How many jars could you inoculate with 6/2000ml flasks of lc?


--------------------
Some do it for the income
But we do it for the outcome
Some of us are active
While others just let their mouths run - Chali 2na & Jurassic 5


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