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OfflineKing0fthajuice
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Registered: 06/28/17
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Loc: Wild Like Volusia in the 80s
Last seen: 1 year, 30 days
Is this Panaeolus Cinctulus
    #26407683 - 12/30/19 12:07 PM (4 years, 30 days ago)

Hey everyone would someone with some microscopy experience give me some advice. 

They seem pretty smooth and elliptic, as well as jet black so I don’t believe them to be Foe’s which are said to be rough and lemon shaped.

Idk I’m still such a novice and I like that this is now bringing me to a new stage of my learning, about spore sizes shapes and terms. Such as I’m looking for elliptic-citrifiorm (that’s what mushroomonserver has for the spores of a Cinctulus

https://imgur.com/gallery/cFKgcFJ
Now that I relook at this set of photos it almost looks like I’m seeing a brown tint to those spores.

This is the habitat of the specimen
https://imgur.com/gallery/L87I9mS

And here are some photos I tried to take that were better then the imgur link.

250x (I think)(next time I’ll make sure to notate the magnification level.)


1000x


400x



This is where it looks brown not jet black and if it’s supposed to be thick cell walled then why can I almost see through it? 

Thanks for any information, also if anyone wants to give me some pointers for next time I know my technique needs tuning.


EDIT: There are more macroscopic photos in my library if these aren’t enough for a positive identification.


--------------------
I like rain

Whether you think you can or you think you can’t, you’re right!


Edited by King0fthajuice (12/30/19 12:09 PM)


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OfflineAlan RockefellerM
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Re: Is this Panaeolus Cinctulus [Re: King0fthajuice] * 1
    #26407755 - 12/30/19 12:57 PM (4 years, 30 days ago)

Definitely Panaeolus, hard to tell which one without lots of microscopy or DNA sequencing.

Black spores look brown with a microscope because some light shines through them.


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OfflineKing0fthajuice
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Registered: 06/28/17
Posts: 325
Loc: Wild Like Volusia in the 80s
Last seen: 1 year, 30 days
Re: Is this Panaeolus Cinctulus [Re: Alan Rockefeller]
    #26407814 - 12/30/19 01:43 PM (4 years, 30 days ago)

Quote:

Alan Rockefeller said:
Definitely Panaeolus, hard to tell which one without lots of microscopy or DNA sequencing.

Black spores look brown with a microscope because some light shines through them.





Thanks Mr. Rockefeller, sir.  I just learned something.  I will continue my research and do my due diligence in documenting. 

I am going to spend some time this evening or in the morning really using the search engine here on shroomery, I’ll admit my impatience when searching first and not giving it my all.  Amazing how just learning about microscopy I can relate it to so many other things in my life like no point committing to something unless willing to be fully committed.


--------------------
I like rain

Whether you think you can or you think you can’t, you’re right!


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OfflineDoc9151M
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Re: Is this Panaeolus Cinctulus [Re: King0fthajuice]
    #26408017 - 12/30/19 03:39 PM (4 years, 30 days ago)

If you want microscopy to be useful, you will need to invest in eye piece reticle so you can measure the different structures. Stage calibration slide is also needed.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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OfflineKing0fthajuice
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Registered: 06/28/17
Posts: 325
Loc: Wild Like Volusia in the 80s
Last seen: 1 year, 30 days
Re: Is this Panaeolus Cinctulus [Re: Doc9151]
    #26408213 - 12/30/19 05:46 PM (4 years, 30 days ago)

Quote:

Doc9151 said:
If you want microscopy to be useful, you will need to invest in eye piece reticle so you can measure the different structures. Stage calibration slide is also needed.





Sweet, I was wondering how I was going to go about measuring the spore length.  Someone had gifted me a usb microscope that attaches to a computer and it came with a calibration tool. 

I'll look those up, thanks Doc!


--------------------
I like rain

Whether you think you can or you think you can’t, you’re right!


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Offlinekushman88
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Re: Is this Panaeolus Cinctulus [Re: King0fthajuice]
    #26410510 - 01/01/20 06:48 AM (4 years, 28 days ago)

You got habitat pics?


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OfflineN0Tmushroom2THINK
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Registered: 04/08/19
Posts: 456
Loc: Vancouver Island, British...
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Re: Is this Panaeolus Cinctulus [Re: King0fthajuice]
    #26410530 - 01/01/20 07:14 AM (4 years, 28 days ago)

https://imgur.com/gallery/L87I9mS
Quote:

King0fthajuice said:
Hey everyone would someone with some microscopy experience give me some advice. 

They seem pretty smooth and elliptic, as well as jet black so I don’t believe them to be Foe’s which are said to be rough and lemon shaped.

Idk I’m still such a novice and I like that this is now bringing me to a new stage of my learning, about spore sizes shapes and terms. Such as I’m looking for elliptic-citrifiorm (that’s what mushroomonserver has for the spores of a Cinctulus

https://imgur.com/gallery/cFKgcFJ
Now that I relook at this set of photos it almost looks like I’m seeing a brown tint to those spores.

This is the habitat of the specimen
https://imgur.com/gallery/L87I9mS

And here are some photos I tried to take that were better then the imgur link.







--------------------
With mushrooms on the mind, you don't have mushroom to think.:mushroomgrow::mushroomgrow:MUSH-LOVE:mushroomgrow::mushroomgrow:


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OfflineDoc9151M
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Registered: 02/23/17
Posts: 13,753
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Re: Is this Panaeolus Cinctulus [Re: N0Tmushroom2THINK]
    #26410550 - 01/01/20 07:43 AM (4 years, 28 days ago)

Quote:

King0fthajuice said:
Quote:

Doc9151 said:
If you want microscopy to be useful, you will need to invest in eye piece reticle so you can measure the different structures. Stage calibration slide is also needed.





Sweet, I was wondering how I was going to go about measuring the spore length.  Someone had gifted me a usb microscope that attaches to a computer and it came with a calibration tool. 

I'll look those up, thanks Doc!



There are computer programs that you can use to measure with that type of microscope,  Alan Rockefeller can tell you or maybe someone else will know and chime in.

Quote:

N0Tmushroom2THINK said:
https://imgur.com/gallery/L87I9mS
Quote:

King0fthajuice said:
Hey everyone would someone with some microscopy experience give me some advice. 

They seem pretty smooth and elliptic, as well as jet black so I don’t believe them to be Foe’s which are said to be rough and lemon shaped.

Idk I’m still such a novice and I like that this is now bringing me to a new stage of my learning, about spore sizes shapes and terms. Such as I’m looking for elliptic-citrifiorm (that’s what mushroomonserver has for the spores of a Cinctulus

https://imgur.com/gallery/cFKgcFJ
Now that I relook at this set of photos it almost looks like I’m seeing a brown tint to those spores.

This is the habitat of the specimen
https://imgur.com/gallery/L87I9mS

And here are some photos I tried to take that were better then the imgur link.









This is Panaeolus cinctulus imo, if the stems are reddish color it's Panaeolus papillionaceus


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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OfflineN0Tmushroom2THINK
NOTmushroom2THINK
Male User Gallery


Registered: 04/08/19
Posts: 456
Loc: Vancouver Island, British...
Last seen: 15 days, 8 hours
Re: Is this Panaeolus Cinctulus [Re: Doc9151]
    #26411301 - 01/01/20 05:39 PM (4 years, 28 days ago)

Agree with ya Doc, :whathesaid:.


--------------------
With mushrooms on the mind, you don't have mushroom to think.:mushroomgrow::mushroomgrow:MUSH-LOVE:mushroomgrow::mushroomgrow:


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OfflineKing0fthajuice
Colors
Male


Registered: 06/28/17
Posts: 325
Loc: Wild Like Volusia in the 80s
Last seen: 1 year, 30 days
Re: Is this Panaeolus Cinctulus [Re: Doc9151]
    #26415266 - 01/03/20 10:11 PM (4 years, 26 days ago)

Quote:

Doc9151 said:
Quote:

King0fthajuice said:
Quote:

Doc9151 said:
If you want microscopy to be useful, you will need to invest in eye piece reticle so you can measure the different structures. Stage calibration slide is also needed.





Sweet, I was wondering how I was going to go about measuring the spore length.  Someone had gifted me a usb microscope that attaches to a computer and it came with a calibration tool. 

I'll look those up, thanks Doc!



There are computer programs that you can use to measure with that type of microscope,  Alan Rockefeller can tell you or maybe someone else will know and chime in.

Quote:

N0Tmushroom2THINK said:
https://imgur.com/gallery/L87I9mS
Quote:

King0fthajuice said:
Hey everyone would someone with some microscopy experience give me some advice. 

They seem pretty smooth and elliptic, as well as jet black so I don’t believe them to be Foe’s which are said to be rough and lemon shaped.

Idk I’m still such a novice and I like that this is now bringing me to a new stage of my learning, about spore sizes shapes and terms. Such as I’m looking for elliptic-citrifiorm (that’s what mushroomonserver has for the spores of a Cinctulus

https://imgur.com/gallery/cFKgcFJ
Now that I relook at this set of photos it almost looks like I’m seeing a brown tint to those spores.

This is the habitat of the specimen
https://imgur.com/gallery/L87I9mS

And here are some photos I tried to take that were better then the imgur link.









This is Panaeolus cinctulus imo, if the stems are reddish color it's Panaeolus papillionaceus





I hope this helps, totally thought I did this but didn’t

https://imgur.com/a/z6uNwYY

STEMS


--------------------
I like rain

Whether you think you can or you think you can’t, you’re right!


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