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OfflineJuanmaki
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Agar Question
    #26407460 - 12/30/19 08:41 AM (4 years, 30 days ago)

I am introducing myself into agar teks and I decided to start with PDA in plastic plates.

I know the recipe and how to poure, but I was wandering what do you do for cleaning the plastic plates, do you sterilize them or do you just clean them with alcohol?

I would prefer not to modify the plates

Thank you for your answers


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OfflineCamera93
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Re: Agar Question [Re: Juanmaki]
    #26407462 - 12/30/19 08:44 AM (4 years, 30 days ago)

PP5 plastic like pastyplates?

or petrie dishes?

Petries that you pour are 1 time use unless you get the glass


pasty plates or other "no pour" plates are sterilized in the PC, so just a nice wipe out with a paper towel before filling and PC'ing is sufficient


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineJuanmaki
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Re: Agar Question [Re: Camera93]
    #26407501 - 12/30/19 09:19 AM (4 years, 30 days ago)

pp5 pastyplates

Thank you Camera,

Is it possible to sterilize unmodified plates whitout getting them wet?

I would like to sterilize PDA and the plates at the same time in the PC and then pourgue the plates in the SAB, so I make sure they're both sterilized and I can do many plates at once.

How can I manage to sterilize many plates at once in the PC?


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OfflineMadcapper144
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Re: Agar Question [Re: Juanmaki]
    #26407512 - 12/30/19 09:29 AM (4 years, 30 days ago)

Here's a good write up on no pours but I'm prettt sure your going to have to modify a little other wise the pressure will mess with your containers. I'm not sure if its just that the lids will pop open or what but I know its not good:shrug:

https://www.shroomery.org/forums/showflat.php/Number/25137693


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Offlinefootpath
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Re: Agar Question [Re: Juanmaki]
    #26407513 - 12/30/19 09:29 AM (4 years, 30 days ago)

You don't pour Pastyplates, you mix and sterilize everything together.
Covering them in foil is the answer to your other questions.

Sounds like you might be confusing terminology or haven't read the teks.


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InvisibleSpunkyMonkey88
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Re: Agar Question [Re: Juanmaki]
    #26407518 - 12/30/19 09:33 AM (4 years, 30 days ago)

Quote:

Juanmaki said:
pp5 pastyplates

Thank you Camera,

Is it possible to sterilize unmodified plates whitout getting them wet?

I would like to sterilize PDA and the plates at the same time in the PC and then pourgue the plates in the SAB, so I make sure they're both sterilized and I can do many plates at once.

How can I manage to sterilize many plates at once in the PC?




Well you cant sterilize anything in a PC with out it getting wet on the outside.

I think you are talking about sterilizing pastyplates while they are empty and sterilizing your media seperate and pouring them in a SAB...

If that's the case then yes it is doable but why dont you just buy petris then?
Either way of you wanna do that you could seal the pp5s and the lids in s vacuum sealer bag. Then u could stack them and pull the whole stack out then open the bag in the SAB and pour them... I do this for sterilizing tools and it works well..


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InvisibleSpunkyMonkey88
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Re: Agar Question [Re: SpunkyMonkey88]
    #26407520 - 12/30/19 09:35 AM (4 years, 30 days ago)

I think he is trying to figure out a way to use miniround with out modifying them, hence, pouring them...

It would work he could just sterilize them while they are empty or stack them and seal them in a vacuum sealer bag...

Not a bad idea if u just dont want to buy petris...


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OfflineCamera93
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Re: Agar Question [Re: SpunkyMonkey88]
    #26407553 - 12/30/19 10:06 AM (4 years, 30 days ago)

If that is the case and he doesn't want to mod anything
then I would suggest the half pint mason jars with plastic 1 piece lids


as others of pointed out, its kinda confusing on what you are looking for

Pour agar = sterilizing the media, and pouring into 1 time use petries that come sterile in package

No pour = using PP5 plastic containers, filling with your yet to be sterilized media, and PC'ing the whole thing with the media already in the plates

*is the use of "media" out of place here lol? Sounds good but it may read like I'm retarded if that's not how its used


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinefootpath
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Re: Agar Question [Re: SpunkyMonkey88]
    #26407561 - 12/30/19 10:13 AM (4 years, 30 days ago)

That sounds like a logistical clusterfuck.
I can't imagine trying to pour even 500mL worth of minirounds, they're just not designed for that kind of work.
Just the handling of the lids is going to fuck you one way or another - whether it's your agar cooling in your media bottle or the amount of time your plates are left exposed.
Even just the space consumption sounds entirely impractical.
I mean, go for it, but I don't see the worth.

Quote:

Camera93 said:
*is the use of "media" out of place here lol? Sounds good but it may read like I'm retarded if that's not how its used




Not at all. 'Agar' is simply just an agar agar-based medium for cell culturing. Collectively, media.
Grain is media. Coir is media. Shit is media.
Canvas, film, paper, air, water...
Media is a bit of a slut of a word.


Edited by footpath (12/30/19 10:34 AM)


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InvisibleSpunkyMonkey88
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Re: Agar Question [Re: footpath]
    #26407585 - 12/30/19 10:39 AM (4 years, 30 days ago)

Quote:

footpath said:
Not at all. 'Agar' is simply just an agar agar-based medium for cell culturing. Collectively, media.
Grain is media. Coir is media. Shit is media.
Canvas, film, paper, air, water...
Media is a bit of a slut of a word.




I love it when you talk dirty


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OfflineJuanmaki
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Re: Agar Question [Re: SpunkyMonkey88]
    #26407838 - 12/30/19 02:03 PM (4 years, 30 days ago)

I don't want to buy petris, I prefer pp plates.

I want to pour pda into pastyplates sterilizing both at the same time in the PC. In that way I can do more agar plates at once, because I can stack the plates in the PC.

Quote:

SpunkyMonkey88 said:

Either way of you wanna do that you could seal the pp5s and the lids in s vacuum sealer bag. Then u could stack them and pull the whole stack out then open the bag in the SAB and pour them... I do this for sterilizing tools and it works well..




Thanks you understood me, I will try that, it's the kind of answer I was looking for. Will it work with a zip pp bag?


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OfflinecronicrFacebook
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Re: Agar Question [Re: Juanmaki]
    #26407853 - 12/30/19 02:17 PM (4 years, 30 days ago)





We used to just stack these and put the containers in one filter patch bag or vac seal bag and lids in another and pour in front of the hood


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OfflineJuanmaki
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Re: Agar Question [Re: Juanmaki]
    #26407856 - 12/30/19 02:18 PM (4 years, 30 days ago)

I'm sorry I think I was confused about the term pouring, i was trying to say to fill the plates with pda. :S Sorry, english is not my native language.


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OfflineJuanmaki
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Re: Agar Question [Re: Juanmaki]
    #26407863 - 12/30/19 02:22 PM (4 years, 30 days ago)

Thank you for your answer cronicr,very clarifying


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OfflinecronicrFacebook
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Re: Agar Question [Re: Juanmaki]
    #26407865 - 12/30/19 02:24 PM (4 years, 30 days ago)

Yeah I can cram 4 times as many in a pc and I don't get condensation issues


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleSpunkyMonkey88
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Re: Agar Question [Re: Juanmaki]
    #26407997 - 12/30/19 03:28 PM (4 years, 30 days ago)


Quote:

Juanmaki said:
I don't want to buy petris, I prefer pp plates.

I want to pour pda into pastyplates sterilizing both at the same time in the PC. In that way I can do more agar plates at once, because I can stack the plates in the PC.

Quote:

SpunkyMonkey88 said:

Either way of you wanna do that you could seal the pp5s and the lids in s vacuum sealer bag. Then u could stack them and pull the whole stack out then open the bag in the SAB and pour them... I do this for sterilizing tools and it works well..




Thanks you understood me, I will try that, it's the kind of answer I was looking for. Will it work with a zip pp bag?




Yes this would work with any bag that wont melt in the PC....


Edited by SpunkyMonkey88 (12/30/19 03:28 PM)


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