Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
InvisiblecoAsTal
Friend
 User Gallery

Registered: 04/04/06
Posts: 2,970
Loc: 8a
Place your bets:
    #26406451 - 12/29/19 02:18 PM (4 years, 1 month ago)

I just sat on my bed, popped open a pre-made agar petrie, and dripped in a drop from a Golden Teacher spore syringe.

Will it contaminate?
Will this contaminate?
You may choose only one


Votes accepted from (12/29/19 11:18 AM) to (No end specified)
View the results of this poll



--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: Place your bets: [Re: coAsTal]
    #26406456 - 12/29/19 02:22 PM (4 years, 1 month ago)

For the record, it is going to show contamination becasue you're introducing dirty ms solution to agar; but you sealed the deal likely anyway.  You can get SOME success without a SAB or FH, but I've never understood the point of wasting resources like that. :shrug::bongload:


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery

Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: Place your bets: [Re: alaskappalachian]
    #26406460 - 12/29/19 02:24 PM (4 years, 1 month ago)

Just for fun :smile:

And in case it changes your mind, I did hold my breath when I put the drop in :wink:


Extras: Filter Print Post Top
OfflineWeiliithinker
Enlightenment Seeker

Registered: 01/13/14
Posts: 206
Last seen: 2 years, 5 months
Re: Place your bets: [Re: coAsTal]
    #26406500 - 12/29/19 02:54 PM (4 years, 1 month ago)

Quote:

coAsTal said:
I did hold my breath when I put the drop in :wink:



  :prayer:


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery

Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: Place your bets: [Re: Weiliithinker]
    #26406770 - 12/29/19 06:54 PM (4 years, 1 month ago)

Stay tuned, I'll post a pic when I see something in the dish...


Extras: Filter Print Post Top
OfflineMadcapper144
Drummer
Male User Gallery

Registered: 01/05/18
Posts: 266
Last seen: 6 months, 9 days
Re: Place your bets: [Re: coAsTal]
    #26406965 - 12/29/19 09:53 PM (4 years, 1 month ago)

Spore syringes are usually dirty in and of themselves but the air in your room is defiantly dirty. Juat to play devils advocate though, when working with agar we transfer several times to eventually get clean plates so technical even if your plate contames you should get some mycelium to transfer. I would transfer under better conditions though like an sab. The laziest shit I've done was nock up brf jars with a ms syringe, everything was set on the open door of my stove I had cocking so the subsequent rising heat kinda acted like a buffer repelling stuff that would otherwise fall on it. Every cake produced w/o contames I did flame the needle though. I wonder if you could do somr agar transfers the same way.:shrug:


--------------------
Buy the ticket, take the ride!! :fearandloathing:


Extras: Filter Print Post Top
InvisibleHans Wermhat
Human


Registered: 06/10/19
Posts: 167
Re: Place your bets: [Re: Madcapper144]
    #26406981 - 12/29/19 10:07 PM (4 years, 1 month ago)

Quote:

Madcapper144 said:
Spore syringes are usually dirty in and of themselves but the air in your room is defiantly dirty. Juat to play devils advocate though, when working with agar we transfer several times to eventually get clean plates so technical even if your plate contames you should get some mycelium to transfer. I would transfer under better conditions though like an sab. The laziest shit I've done was nock up brf jars with a ms syringe, everything was set on the open door of my stove I had cocking so the subsequent rising heat kinda acted like a buffer repelling stuff that would otherwise fall on it. Every cake produced w/o contames I did flame the needle though. I wonder if you could do somr agar transfers the same way.:shrug:



kind of like in biology lab back in school we'd pour and inoculate our plates next to bunsen burners in the open air, the logic being the flame would keep all the baddies from landing on the agar. i've always wondered how effective the method actually is. definitely easier to work with than a sab.


--------------------
Thank you for taking the time to read this message. Have a wonderful day.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Place your bets: [Re: Hans Wermhat]
    #26406992 - 12/29/19 10:17 PM (4 years, 1 month ago)

Brf cakes can be inoculated  because they have a dry verm layer. 13  years ago we did it on the kitchen counter and rarely ever had any issues.
Bunsen burners are great for cultures you plan to keep for a week but a sab is super effective and far cheaper


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery

Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: Place your bets: [Re: Madcapper144]
    #26407373 - 12/30/19 07:19 AM (4 years, 30 days ago)

Quote:

Madcapper144 said:
Spore syringes are usually dirty in and of themselves but the air in your room is defiantly dirty. Juat to play devils advocate though, when working with agar we transfer several times to eventually get clean plates so technical even if your plate contams you should get some mycelium to transfer. I would transfer under better conditions though like an sab. :




Indeed-- should I get anything salvageable I will transfer it to a sterile plate in a still-air.


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery

Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: Place your bets: [Re: coAsTal] * 1
    #26427687 - 01/11/20 08:22 AM (4 years, 18 days ago)

Update:

Thoughts?



--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Place your bets: [Re: coAsTal]
    #26427695 - 01/11/20 08:27 AM (4 years, 18 days ago)

looks about right.


Extras: Filter Print Post Top
InvisibleTedsDead
 User Gallery

Registered: 01/03/17
Posts: 4,998
Trusted Cultivator
Re: Place your bets: [Re: verum subsequentis]
    #26427712 - 01/11/20 08:48 AM (4 years, 18 days ago)

looks like your dab container  contamed:shrug:


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery

Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: Place your bets: [Re: TedsDead]
    #26427716 - 01/11/20 08:51 AM (4 years, 18 days ago)

And thus the lesson endeth...

:bubbles:


Extras: Filter Print Post Top
InvisibleHans Wermhat
Human


Registered: 06/10/19
Posts: 167
Re: Place your bets: [Re: coAsTal]
    #26428440 - 01/11/20 04:11 PM (4 years, 18 days ago)

Quote:

coAsTal said:
Update:

Thoughts?





If you look closely there appears to be some contamination at the center of the plate and around the edge of the plate, although I don't have my reading glasses on and I'm viewing the image thru braille so I could be mistaken.


--------------------
Thank you for taking the time to read this message. Have a wonderful day.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Agar Spore Transfer: How am I doing?
( 1 2 all )
superwhitedolemite 1,087 38 01/24/20 05:42 PM
by superwhitedolemite
* Outdoor G2G Transfer Experiment... mrr0gers 1,734 7 05/25/03 09:19 PM
by ChrisH
* How to make agar 2 agar transfer? esp 6,652 7 01/08/04 11:08 AM
by YidakiMan
* What is my safest bet? Chemical_Smile 719 7 04/28/03 11:30 AM
by Chemical_Smile
* G2G transfers
( 1 2 all )
kjb1891 32,777 20 12/19/18 11:45 AM
by Nichrome
* G2G Transfer? lvleph 4,456 9 11/20/02 12:28 PM
by scifi5001
* substrate transfer?!?! dets 2,910 8 11/13/02 12:29 AM
by SixTango
* G2G transfers? lvleph 4,142 15 11/03/13 06:15 AM
by SpitballJedi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
410 topic views. 20 members, 218 guests and 54 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.059 seconds spending 0.04 seconds on 20 queries.