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InvisiblePainfullyaware1973
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Registered: 10/31/19
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Are these spores contamanated?
    #26405570 - 12/29/19 01:04 AM (4 years, 1 month ago)

I recently purchased a penis envy spore syringe and before inoculating a few grain jars I used a tiny bit in an Agar plate to try and get a culture going. A few days passed and I noticed two small green molds but the rest of it looked ok. After cutting out the two small green molds I checked on it the next day and there is now green mold everywhere and it appears all these separate green molds are in fact the penis envy spores themselves. Does anyone know if this means that the spores themselves are contaminated? This is the first time I have ordered from www.mushrooms.com, anyone ever had any problems with this vendor? Any thoughts guys?


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Offlinemitchell_danger
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Re: Are these spores contamanated? [Re: Painfullyaware1973]
    #26405658 - 12/29/19 03:31 AM (4 years, 1 month ago)

Entirely possible that your technique is improper. PE should appear white on agar.


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OfflineMrBovineJoni
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Re: Are these spores contamanated? [Re: mitchell_danger]
    #26405698 - 12/29/19 05:08 AM (4 years, 1 month ago)

It is also very possible that you have spread mold spores around the petri dish while removing those 2 contaminated spots - it looks like a thorough contamination. There could by mycelium hiding in a few spots but it would still take a few transfers to get it cleaned up.

How much of the syringe spore liquid did you use? One drop should be enough if the syringe has been properly shaken (and spores are not all clumped up at the bottom) - it can then be spread around in one of the common pattern (zig-zag, S, $ and so on).


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Invisibletramalot
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Registered: 12/13/19
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Re: Are these spores contamanated? [Re: Painfullyaware1973]
    #26407113 - 12/30/19 01:02 AM (4 years, 30 days ago)

I newb but I think the idea is when doing the petri dish thing is it to get it going and then take some Myc and put it in another dish to get it away from the bad stuff and repeat a few times possibly maybe

But anyway best of luck and thanks for sharing your troubles to help us noobs learn


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InvisiblePainfullyaware1973
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Re: Are these spores contamanated? [Re: tramalot]
    #26411148 - 01/01/20 03:44 PM (4 years, 28 days ago)

I have started a few cultures with some Myc and the results are 100% better then using spores from a syringe...but when i only have spores from certain strains i really have no choice. I had contamination before but it was always a few spots here and there separate from the Myc i had growing. This was the first time the spores themselves had started molding.


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InvisiblePainfullyaware1973
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Re: Are these spores contamanated? [Re: tramalot]
    #26411168 - 01/01/20 03:59 PM (4 years, 28 days ago)

I did manage to get some strong Myc growing in a few dishes but it was from some of the Shrooms i had cut open from my first grow and put a few pieces in the dish.The results are amazing compared to doing the same with spores. I managed to get 190 Grams dry from my first grow attempt.


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