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ShamanBoof
Undergraduate EECS



Registered: 08/25/19
Posts: 131
Loc: France
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Re: Let's grow some mold [Re: Kizzle]
#26405479 - 12/28/19 10:40 PM (4 years, 1 month ago) |
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Luminous7


Registered: 01/26/16
Posts: 1,416
Last seen: 3 hours, 14 minutes
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( Pics from a few years ago. ) Since iv learned agar and do bulk with coir verm and gypsum via bucket tek , I rarely get contamination in my tubs and ill pull up to 7 flushes before seeing trich or anything weird. Maybe it is the brand of coir? I always thought it was processed at high temps so it kind of was already pasturized?
That was a cool video about the Coco coir !





Edited by Luminous7 (12/28/19 11:28 PM)
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Luminous7


Registered: 01/26/16
Posts: 1,416
Last seen: 3 hours, 14 minutes
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Re: Let's grow some mold [Re: Buddaking]
#26405517 - 12/28/19 11:32 PM (4 years, 1 month ago) |
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Quote:
Buddaking said:
Quote:
cronicr said: Yeah of cakes have the best biological efficiency
I have seen cakes going well over 300% BE on multiple flushes.. I will always keep a bag of brown rice around. I would clone clusters from MS cakes & run my sectors from their in tubs. Seemed to work well & cut my time, space & energy usage to desired genetic traits in master slants.
I like that process. No need to do a huge bulk just to get cloning strains.
Although with a bigger MS tub would mean more Gene variance to choose a clone from?
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murderlabz
RIP Stoneman



Registered: 05/18/19
Posts: 551
Loc: The Multiverse
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Re: Let's grow some mold [Re: Luminous7]
#26405546 - 12/29/19 12:12 AM (4 years, 1 month ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Yeah I pc'd my coir pasteurized it bucket tek u name it. Even if trich survives pasturizing it doesn't like to germinate on coir
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: Let's grow some mold [Re: cronicr]
#26405550 - 12/29/19 12:20 AM (4 years, 1 month ago) |
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I likely just found a shitty brand of coir with lots of impurities and had dirty cultures which would make 5 different cube cultures suspect. This last run was sfd's for filters poly and no mod lids. I even swabbed my filter n it showed nothing on agar
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 7 hours
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Re: Let's grow some mold [Re: cronicr]
#26405557 - 12/29/19 12:31 AM (4 years, 1 month ago) |
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Or shitty batch anyway. I'm not sure how relevant brand is. These places that distribute coir bricks most likely get them from a large number of different sources and just slap a label on them so you never really know what you're getting.
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 2 days
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Re: Let's grow some mold [Re: Kizzle]
#26405601 - 12/29/19 02:11 AM (4 years, 1 month ago) |
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hey cron have u noticed it in both brick and shredded form?? i bought a bag yesterday of eco earth in a 8qt shredded bag and it looked pretty good actually. at least compared to the last bricks of eco i had . the shredded shit seemed darker and felt nice and fluffy. as i stated before i quit buyin eco bricks a while back for the same reason and switched to this generic shit in 14 cubic ft. bricks from the hydro store here. so far that has been fine but as someone said who knows where they get the shit from. i have very limited space and only ever run no more than 4 monos at a time so that big ass brick will last me a good god damn long time but ima use the shredded eco bag next just to see how it does. idk the shit is frustrating tho i dont get how shit works great for years on end then all of a sudden its shit
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 2 years, 7 days
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Re: Let's grow some mold [Re: jcm4620]
#26405678 - 12/29/19 04:33 AM (4 years, 1 month ago) |
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As others suggested, try your cultures with verm only, straw or cakes. Let’s see if they contaminate. It can easily happen that your coir has nutritional shit, yesterday I picked out a few things from coir which looked like seed hulls.
If this does not work it has to be the culture. The same exact thing happened to me which was the reason I took a break from cult. Every tub I made had trich no matted what I did. I swapped sfd, took extra attention with sterile work, I even started fucking pasteurising and spawn in front of the flowhood. Nothing worked and I just kept throwing out subs after subs. What I did not do is change culture as I had a killer clone and did not want to start over. I just couldn’t understand how my cultures look clean but still greenz.
Cultures may look clean on agar and may look clean on grain, but it is very easy to miss a hidden contam. Swipe some spores transfer a few, drop it on grain and see if you still get green with clean fresh culture. I know it is super annoying and easy to blame everything else, but if you have clean culture you can basically half ass everything and still get shrooms.
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Luminous7


Registered: 01/26/16
Posts: 1,416
Last seen: 3 hours, 14 minutes
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Re: Let's grow some mold [Re: eLeSDenes]
#26406071 - 12/29/19 10:27 AM (4 years, 1 month ago) |
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FUck I got Mucor pin mold in my eringii bin

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sporealchemy
ok


Registered: 04/10/11
Posts: 401
Loc: ( ͡° ͜ʖ ͡°)
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Re: Let's grow some mold [Re: eLeSDenes]
#26406083 - 12/29/19 10:34 AM (4 years, 1 month ago) |
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I had to send back an order of coir recently bc it looked kind of funny but after I hydrated it, it smelled like shit. Musty and sour. I did a boiling bucket tek too. It was eco earth. Actually I explained that it couldn’t be sent back since I hydrated it and the package weight had increased by a lot lol. I said it would make my reptiles sick and they sent another which turned out to be not fucked up. I also grabbed some growit! brand which is one big brick and slightly cheaper per lb. It seems to be working just as well and holds the same amount of water but it was a lot lighter in color. I haven’t tried the big walmart bricks yet.
As for cultures, I believe that molds can ride along for a while and be invisible. I’m not sure how they infiltrate the myc and hold on for so long. Hate that shit. My ego was big when I first started and I thought that I needed to buy an ozone generator when my first few tubs contamed bc I thought my technique was perfect. In hindsight maybe running an ozone generator could have helped to run one time before growing anything since the house was so old and moldy. My main issue was me just being a noob though.
-------------------- "Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want."-RR

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Mariochi64
Lous daed



Registered: 09/28/18
Posts: 101
Loc: 7 years 10 months..
Last seen: 3 years, 2 months
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Does anyone know what this mold is called? It showed up in my agar plates and my myc WOULD NOT GO ANYWHERE NEAR IT like it formed an invisible wall of some sort
 Sorry for the flash I couldn’t get the lighting right.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: Let's grow some mold [Re: eLeSDenes]
#26406226 - 12/29/19 11:51 AM (4 years, 1 month ago) |
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Quote:
eLeSDenes said: As others suggested, try your cultures with verm only, straw or cakes. Let’s see if they contaminate. It can easily happen that your coir has nutritional shit, yesterday I picked out a few things from coir which looked like seed hulls.
If this does not work it has to be the culture. The same exact thing happened to me which was the reason I took a break from cult. Every tub I made had trich no matted what I did. I swapped sfd, took extra attention with sterile work, I even started fucking pasteurising and spawn in front of the flowhood. Nothing worked and I just kept throwing out subs after subs. What I did not do is change culture as I had a killer clone and did not want to start over. I just couldn’t understand how my cultures look clean but still greenz.
Cultures may look clean on agar and may look clean on grain, but it is very easy to miss a hidden contam. Swipe some spores transfer a few, drop it on grain and see if you still get green with clean fresh culture. I know it is super annoying and easy to blame everything else, but if you have clean culture you can basically half ass everything and still get shrooms.
Yeah I just spawned some to straw so we will see but that would make 6 cultures all cubensis that have the same mold hiding in them
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ShamanBoof
Undergraduate EECS



Registered: 08/25/19
Posts: 131
Loc: France
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Re: Let's grow some mold [Re: cronicr]
#26407015 - 12/29/19 10:45 PM (4 years, 1 month ago) |
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Shit got another green jar figured out y though, it's because this stupid red silicone not sticking. Have no idea what to use instead for my LC jars
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Ditch the syringe filters save those for lc( even those I go un modded
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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ShamanBoof
Undergraduate EECS



Registered: 08/25/19
Posts: 131
Loc: France
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Re: Let's grow some mold [Re: cronicr]
#26407038 - 12/29/19 11:14 PM (4 years, 1 month ago) |
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Quote:
cronicr said: Ditch the syringe filters save those for lc( even those I go un modded
Yeah I use them for LC but couldn't find normal lid.. How do you glue them?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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With a proper sized drill bit it's a nice tight fit.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: Let's grow some mold [Re: cronicr]
#26408034 - 12/30/19 03:48 PM (4 years, 1 month ago) |
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So this is knotting up so in theory it should show green anytime now
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Let's grow some mold [Re: cronicr]
#26408064 - 12/30/19 04:06 PM (4 years, 1 month ago) |
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Sloppy sterile tek =
-------------------- Tek's I use LAGM2020
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 2 hours, 52 minutes
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Re: Let's grow some mold [Re: cronicr]
#26408081 - 12/30/19 04:20 PM (4 years, 1 month ago) |
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Quote:
cronicr said: With a proper sized drill bit it's a nice tight fit.
5/32" for all of the one's I get. You know I had about 40 of these and was too lazy to buy RTV for new lids, so I just started using these...and they work damn well.
When I get around to getting some RTV, I can just slap down some SFD on the hole I made for these.
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