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Offlinemagicschoolbus
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Registered: 02/19/18
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LC Premix for Agar?
    #26402775 - 12/27/19 09:44 AM (4 years, 1 month ago)

I've been using this LC Premix to make agar.

The recipe calls for 1 teaspoon per 1000ml of water used, so I've been using 1/2 teaspoon in my 500ml media bottle to make agar for petri dishes.

I've done a few transfers of several strains and haven't noticed any abnormalities in what you'd expect in terms of growth, but I was wondering...

1/2 teaspoon of the LC premix comes out to only around 1.5g of media, but most agar recipes call for 10g of media for 500ml of water.

Would it be beneficial to instead use 10g of the LC premix in my agar, rather than the ~1.5g that I've been using? Would there be any change in mycelium performance/appearance?

I don't even know what's in the LC premix.


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:trippinballs::trippinballs:


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OfflineBarrel
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Registered: 10/27/18
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Re: LC Premix for Agar? [Re: magicschoolbus] * 1
    #26403727 - 12/27/19 09:32 PM (4 years, 1 month ago)

If it's working, it's working. You've got an assload of LC mix and agar is cheap, so experiment if you want. As you add more you might see some issues with agar clarity. If you aren't seeing any stalling at 1.5g there probably isn't much to be gained by adding more. Mycelium really only grows along the surface of agar, so you'll probably just be making more nutritious trash up around 10g.


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Invisiblemurderlabz
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Registered: 05/18/19
Posts: 551
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Re: LC Premix for Agar? [Re: Barrel]
    #26403808 - 12/27/19 10:51 PM (4 years, 1 month ago)

I was using my Karo/Honey LC recipe for Agar. It's not killer but works.




I an now trying out mushboy's 695 BRF Agar (6g brf 9g telefone 500ml tap water) which is based off of Bod's BRF Agar test runs. I just want to use shit I already got.


Edited by murderlabz (12/27/19 10:55 PM)


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