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OfflineBobBee
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PE spores to agar: what am I looking for/at?
    #26400256 - 12/25/19 09:15 AM (4 years, 1 month ago)

Of the 12 no pour dishes I’ve made, I saved four that I thought looked promising. I did a zigzag pattern with my spore tool and something is definitely happening. There is white blobs forming but also white fluffy stuff and I don’t know if it’s all the same. I’ve looked through the library gallery at Agar pictures but I’m still confused of what’s mycelium and what isCobweb mold. How can I tell the difference?


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OfflineNeowynd8
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Re: PE spores to agar: what am I looking for/at? [Re: BobBee]
    #26400264 - 12/25/19 09:23 AM (4 years, 1 month ago)

If there is mold, it's prolly not cobweb. Cobweb grows very quickly. It would prolly cover the whole plate and climb the walls in a single day. Try to get better pics


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OfflineRandalf the Grey
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Re: PE spores to agar: what am I looking for/at? [Re: BobBee]
    #26400268 - 12/25/19 09:26 AM (4 years, 1 month ago)

Looks possibly bacterial, but can't say for sure. Let it grow a bit a see how it looks.

I am confused about the saved 4 plates comment though. Do you mean you swabbed several and 4 looked worth saving, or that only 4 of the unused no pours looked good?


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Randalf the Grey
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Invisiblepablokabute
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Re: PE spores to agar: what am I looking for/at? [Re: Randalf the Grey]
    #26400275 - 12/25/19 09:33 AM (4 years, 1 month ago)

looks like shit

ditch the plates and start all over


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OfflineBobBee
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Re: PE spores to agar: what am I looking for/at? [Re: Randalf the Grey]
    #26400354 - 12/25/19 10:56 AM (4 years, 1 month ago)

Thanks for your comment, Randalf. The plates that I discarded looked like they had growth in areas where I did not perform the zigzag motion with my loop tool. They had slimy spots on them. I also needed the jars for my next round of agar! Sidenote: I need to buy more jars


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OfflineRandalf the Grey
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Re: PE spores to agar: what am I looking for/at? [Re: BobBee]
    #26400484 - 12/25/19 01:22 PM (4 years, 1 month ago)

Gotcha. Yeah, slimy spots in the others is a sign of bacteria. If the others were contamed, it is possible they all were. May be the plates that are the issues, not the spores.
I would say try making more plates. If only 4 out of 12 survived then something went wrong.


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OfflineBobBee
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Re: PE spores to agar: what am I looking for/at? [Re: Randalf the Grey]
    #26405016 - 12/28/19 04:27 PM (4 years, 1 month ago)

I transferred from that plate a few days ago and this is what I got on one of my plates that I transferred. I’m transferring this again tonight but curious does it look like a cube mycelium? Or am I transferring mold? LOL


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InvisibleTormatoMFacebookDiscordReddit
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Re: PE spores to agar: what am I looking for/at? [Re: BobBee]
    #26405023 - 12/28/19 04:31 PM (4 years, 1 month ago)

Quote:

BobBee said:
I transferred from that plate a few days ago and this is what I got on one of my plates that I transferred. I’m transferring this again tonight but curious does it look like a cube mycelium? Or am I transferring mold? LOL



Looks bacterial and just nasty :uhoh:


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OfflineA.k.aM
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Re: PE spores to agar: what am I looking for/at? [Re: Tormato]
    #26405058 - 12/28/19 05:00 PM (4 years, 1 month ago)

Your dish got a good pump in at the gym, check out the vascularity. Lol

That’s really cool looking but unfortunately not in the way you want.


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Invisiblehummingbird

Registered: 06/29/14
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Re: PE spores to agar: what am I looking for/at? [Re: A.k.a]
    #26405070 - 12/28/19 05:09 PM (4 years, 1 month ago)

Looks like slime mold and bacteria... with maybe a little cube myc mixed in.


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