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InvisibleSpunkyMonkey88
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Shaking LC jars with a syringe filter
    #26398652 - 12/24/19 05:49 AM (4 years, 1 month ago)

I just shoke the shit out of one of my jars and noticed a couple drops came out of the syringe filter.

I'm wondering if the LME saturated filter could be a wick for contaminants or if it doesn't even matter if something Is growing on the outside if it because it's a .22 micron filter.

I mean they are made to filter out bad shit right?


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InvisibleFeasoghorm

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Re: Shaking LC jars with a syringe filter [Re: SpunkyMonkey88]
    #26399375 - 12/24/19 04:10 PM (4 years, 1 month ago)

Yes, but i wud imagine that if it cud be saturated (wet nutes) then shit cud grow through it. I thnk it's one of them possible but not probable things.


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OfflineSFS96
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Re: Shaking LC jars with a syringe filter [Re: Feasoghorm]
    #26399395 - 12/24/19 04:25 PM (4 years, 1 month ago)

Most people swirl lc jars and try to not get the filter wet. Im no LC expert but im pretty sure it can cause problems.


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OfflinecronicrFacebook
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Re: Shaking LC jars with a syringe filter [Re: SFS96] * 1
    #26399399 - 12/24/19 04:28 PM (4 years, 1 month ago)

Slow down there 007 we like it stirred not shaken


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InvisibleSpunkyMonkey88
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Re: Shaking LC jars with a syringe filter [Re: cronicr]
    #26399591 - 12/24/19 07:16 PM (4 years, 1 month ago)

Ya I shook them both pretty hard in order to break up the mycelium since I dont have a stir plate...

I thought that was common practice?

I'm not even gonna bother using them if theres a chance that a wet syringe filter could wick contams into the jar...

Thanks guys.


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OfflineSFS96
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Re: Shaking LC jars with a syringe filter [Re: SpunkyMonkey88]
    #26399835 - 12/25/19 12:03 AM (4 years, 1 month ago)

Most people put some glass or a marble inside so the mycelium easily breaks apart when it’s swirled around.


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InvisibleSpunkyMonkey88
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Re: Shaking LC jars with a syringe filter [Re: SFS96]
    #26400144 - 12/25/19 07:35 AM (4 years, 1 month ago)

Quote:

SFS96 said:
Most people put some glass or a marble inside so the mycelium easily breaks apart when it’s swirled around.




Ya that's what I did I thought it was so I could shake it like a spray paint can 🤪

The last time I did lcs I used marbles and had 3 out of 4 jars break from shaking them...that should tell ya something...


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Invisiblegizmo1
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Re: Shaking LC jars with a syringe filter [Re: SpunkyMonkey88]
    #26400148 - 12/25/19 07:42 AM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Quote:

SFS96 said:
Most people put some glass or a marble inside so the mycelium easily breaks apart when it’s swirled around.




Ya that's what I did I thought it was so I could shake it like a spray paint can 🤪

The last time I did lcs I used marbles and had 3 out of 4 jars break from shaking them...that should tell ya something...



Hahahahahaha


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InvisibleLadysKnight
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Re: Shaking LC jars with a syringe filter [Re: gizmo1]
    #26400195 - 12/25/19 08:21 AM (4 years, 1 month ago)

Doesn't really need a filter or marbles. Then you can shake the shit out of it.


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OfflineNeowynd8
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Re: Shaking LC jars with a syringe filter [Re: SpunkyMonkey88]
    #26400270 - 12/25/19 09:29 AM (4 years, 1 month ago)

Test your lc on an agar plate or single jar and proceed from there. If you get a clean plate, roll with it.


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Invisiblesporealchemy
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Re: Shaking LC jars with a syringe filter [Re: Neowynd8]
    #26400282 - 12/25/19 09:42 AM (4 years, 1 month ago)

Lol I used to use broken beer bottle shards. Now I just swirl it pre PC to check the volume inside to make sure it isn’t gonna touch the filter when I swirl it after inoculation once a day or so. 1/4-1/3 full usually does the trick :wink:


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InvisibleSpunkyMonkey88
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Re: Shaking LC jars with a syringe filter [Re: sporealchemy]
    #26401326 - 12/26/19 08:28 AM (4 years, 1 month ago)

So anyways... would you guys use that lc that had a little liquid drip out the syringe filter?

Or does that seem too risky?


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Invisiblesporealchemy
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Re: Shaking LC jars with a syringe filter [Re: SpunkyMonkey88]
    #26401482 - 12/26/19 11:13 AM (4 years, 1 month ago)

If it’s a whatman and you are confident in how you fixed it to the lid, it’s probably fine:thumbup:
If this happened with a SFD filter I’d say no thank you.


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OfflineAnticode
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Re: Shaking LC jars with a syringe filter [Re: sporealchemy]
    #26401588 - 12/26/19 12:51 PM (4 years, 1 month ago)

I had a honey tek LC jar that had no contams in the beginning, that after my micropore tape got wet(swirled too hard), the next time I used it, it was contaminated on my grain and agar (pink contam). I'm not sure if syringe filters would do the same, but the multiple layers of micropore tape allowed contam in when wet.

Hope this helps. Best of luck.


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InvisibleSpunkyMonkey88
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Re: Shaking LC jars with a syringe filter [Re: Anticode]
    #26401646 - 12/26/19 02:03 PM (4 years, 1 month ago)

Quote:

Anticode said:
I had a honey tek LC jar that had no contams in the beginning, that after my micropore tape got wet(swirled too hard), the next time I used it, it was contaminated on my grain and agar (pink contam). I'm not sure if syringe filters would do the same, but the multiple layers of micropore tape allowed contam in when wet.

Hope this helps. Best of luck.




Syringe filters are made to filter contams out of liquids though so it really doesn't even compare to micropore tape which isn't even a filter and doesn't have a standard pore size...

Thanks though


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OfflineAnticode
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Re: Shaking LC jars with a syringe filter [Re: SpunkyMonkey88]
    #26405822 - 12/29/19 07:25 AM (4 years, 1 month ago)

Thanks monkey. I wasn’t sure if they compared to micropore tape. Ive also purchased syringe filters but haven’t used them yet. Just wanted to share my experience with micropore tape in case they are similar. Thanks for the clarification.


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Invisiblesporealchemy
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Re: Shaking LC jars with a syringe filter [Re: Anticode]
    #26406113 - 12/29/19 10:51 AM (4 years, 1 month ago)

I’ve never messed w syringe filters but they are theoretically the shit. Like Monkey said, the filtration effectivity is standard and specified for each unit.
As for MP tape, it’s about as effective as toilet paper.
https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=13642707


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OfflinecronicrFacebook
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Re: Shaking LC jars with a syringe filter [Re: sporealchemy]
    #26406527 - 12/29/19 03:14 PM (4 years, 1 month ago)

Stonesuns pics were flawed that hole thread is lol.


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InvisibleSpunkyMonkey88
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Re: Shaking LC jars with a syringe filter [Re: Anticode]
    #26406650 - 12/29/19 05:07 PM (4 years, 1 month ago)

Quote:

Anticode said:
Thanks monkey. I wasn’t sure if they compared to micropore tape. Ive also purchased syringe filters but haven’t used them yet. Just wanted to share my experience with micropore tape in case they are similar. Thanks for the clarification.




Ya I use syringe filters and a manufactured SHIP that is used for medical vials. The syringe filter is only so you can draw out a given amount of liquid without creating a vacuum...


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Re: Shaking LC jars with a syringe filter *DELETED* [Re: SpunkyMonkey88]
    #26406671 - 12/29/19 05:28 PM (4 years, 1 month ago)

Post deleted by SannyTIES

Reason for deletion: Shit post


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InvisibleSpunkyMonkey88
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Re: Shaking LC jars with a syringe filter [Re: SannyTIES]
    #26406695 - 12/29/19 05:51 PM (4 years, 1 month ago)

Quote:

SannyTIES said:
How are you securing the filter disk to the lid?
I use them also. Same set up your describing.  Well same equipment.  When I use metal lids I use clear silicone to seal the hole I punch with a paper hole punch.  Perfect size, the port pops in too. I recently went to plastic pp5 lids and due to polypropylene being hard to adhere to ,I use in jb plastic weld to secure the filter. Sandpaper both surfaces and apply. Also I leave the lid  loose while pressure cooking.
I do this because  I had leaks around the filter disk. Now what use is all the filter if we have a leak around it. Lol.






I was having the same problem with the pp5 and silicone. I'm using metal lids but my syringe filter housing is made out of polyurethane and slips right out of the rtv silicone.

So I made a hole slightly smaller then my syringe filter, heated up the lid with a lighter and forced the syringe filter through the hole while it is red hot making a tight fit. Then I applied a liberal amount of RTV silicone to the underside of the lid around the filter.

I think its doing ths trick but I have to be extremely careful not to bumpt the syringe filter because the melted seal keeping it tight can easily be broke resulting in a leaky filter... it sucks but it's the best I can do with them.

I ordered some other ones that were made out of abs but they just get deformed in the PC...

Do you have a link to the filters you use?

I'll have to try JB weld...your saying it bonds to the pp5 well?



Edited by SpunkyMonkey88 (12/29/19 05:53 PM)


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Invisiblesporealchemy
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Re: Shaking LC jars with a syringe filter [Re: cronicr]
    #26406803 - 12/29/19 07:42 PM (4 years, 30 days ago)

Quote:

cronicr said:
Stonesuns pics were flawed that hole thread is lol.



Lol you don’t like it?


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InvisibleSpunkyMonkey88
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Re: Shaking LC jars with a syringe filter [Re: sporealchemy]
    #26406821 - 12/29/19 07:57 PM (4 years, 30 days ago)

Quote:

sporealchemy said:
Quote:

cronicr said:
Stonesuns pics were flawed that hole thread is lol.



Lol you don’t like it?




Ya what do u mean? Are you advocating micropore tape as an effective filter?

Now that you say it, I always used one layer of micropore tape ontop of a hole in my PP5'S and I never had issues with contamination....maybe it behaves differently on a grain jar that gets shook though...

I'd imagine if the grains got the filter starchy and moist from shaking it, something could germinate and grow through it...


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