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InvisibleLadysKnight
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Tiny bubbles on mycelium?
    #26398039 - 12/23/19 06:01 PM (4 years, 1 month ago)

Using a visor with 10x magnifying lenses, on a couple agar plates, I can see what look like tiny bubbles at the tips of the branching mycelium.

I can only say for sure that they are tiny shiny dots. Guessing they could be air bubbles or water bubbles or secreting metabolites.

First time paying this much attention, would love a clue as to what they are telling me.


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Offlinespiritlands
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Re: Tiny bubbles on mycelium? [Re: LadysKnight]
    #26398427 - 12/23/19 11:50 PM (4 years, 1 month ago)

In my experience shiny dots at the tip of the Mycelium it's mold. Esp if there is a ring of longer mycelium around the edges and the shiny dots are in the shorter mycelium in a patch in the middle of the colony. The dots will take on color as the surrounding Mycelium gets thicker and will have a more glass like appearance instead of snow white. Shiny dots will usually turn yellow or green.

I have a rw pitri that this happened to at home right now. It's waiting to be thrown out.
For example this is pan cyan mycelium↓ 1000xzoom

This is trich before spores develope color and mature↓ 1000xzoom


I have to reset the resolution on my microscope so my pics are bigger but I can take a pic of it when I get home.


Edited by spiritlands (12/24/19 12:01 AM)


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OfflineKizzle
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Re: Tiny bubbles on mycelium? [Re: spiritlands]
    #26398478 - 12/24/19 01:11 AM (4 years, 1 month ago)

Like this?


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InvisibleLadysKnight
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Re: Tiny bubbles on mycelium? [Re: spiritlands]
    #26398797 - 12/24/19 08:03 AM (4 years, 1 month ago)

@Spiritlands - even though your pics are too small for me to see, your description is accurate. So this sounds like an early sign of mold. I will watch these.

@ Kizzle - At this point they are still clear, and only on one sector of the plate, and fewer.


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Offlinejcm4620
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Re: Tiny bubbles on mycelium? [Re: LadysKnight]
    #26398830 - 12/24/19 08:36 AM (4 years, 1 month ago)

pin mold


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InvisibleTormatoMFacebookDiscordReddit
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Re: Tiny bubbles on mycelium? [Re: LadysKnight]
    #26398832 - 12/24/19 08:37 AM (4 years, 1 month ago)

@ LadysKnight: Can you post pics? Nice username btw :lol:


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InvisibleLadysKnight
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Re: Tiny bubbles on mycelium? [Re: Tormato]
    #26398845 - 12/24/19 08:49 AM (4 years, 1 month ago)

I've tried taking pics, but couldn't capture the dots. They are almost imperceptable at 10x.


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OfflineElrik
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Re: Tiny bubbles on mycelium? [Re: LadysKnight]
    #26398981 - 12/24/19 10:49 AM (4 years, 1 month ago)

I use my 10X hand lens to check baby colonies for round nodules on the ends of the hyphae so I can spot mould colonies in the first days of growth after putting spores to agar. But I also see 'bubbles' in clean and healthy mycelium.
Here is an image of Psi. mexicana captured at 40X

This is a clean and healthy culture, no mould or bacteria, and the 'bubbles' are suspended within the mycelial mass, not in the agar.
I think they are either metabolites or an insurance against gusts of dangerously dry air.
The nodules on mould look quite different, thankfully.


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InvisibleLadysKnight
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Re: Tiny bubbles on mycelium? [Re: Elrik]
    #26399002 - 12/24/19 11:16 AM (4 years, 1 month ago)

Interesting. So there are good and bad "bubbles". Would you happen to have any pics at 10x for comparison?


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OfflineElrik
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Re: Tiny bubbles on mycelium? [Re: LadysKnight]
    #26399778 - 12/24/19 10:50 PM (4 years, 1 month ago)

I tried to take pics but they are just barely visible at 10X and didn't photograph. To my eye they glistened like dew.
Thankfully, this phenomena really looks nothing like the nodules that form on the end of mould hyphae. Once you look at mould under even 4X magnification several times you should become very good at spotting most types of it.
Even with my naked eye, mould has a visual texture that often stands out enough to make me get the magnifying glass. I've saved several colonies of actives by being able to identify moulds before they sporulate due to this.


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