Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Mushroom-Hut Mono Tub Substrate   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
OfflineKmacmo
The aborted pin
Other


Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 8 hours, 15 minutes
Cubensis spores germinating on agar(pics), need opinions good or bad?
    #26395530 - 12/22/19 12:17 PM (4 years, 1 month ago)

Hey people, These 3 plates were inoculated with cubensis spores on 13th december.
Is it mycelium starting to grow? Its translucent at the moment but usually turns white and fluffy after a while

The black spots are clumps of spores.
6th picture along in the first row, the bubbly looking area at the top is where the loop cooled into the agar (ill keep an eye on it)



These were inoculated on the 5th december and they looked very similar to how the plates about are beginning to look, anything worth transfering on these?



--------------------


Edited by Kmacmo (12/22/19 12:24 PM)


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
Trusted Cultivator
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: Kmacmo]
    #26395543 - 12/22/19 12:25 PM (4 years, 1 month ago)

why would u start another thread on the same thing???????


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
OfflineKmacmo
The aborted pin
Other


Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 8 hours, 15 minutes
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: jcm4620]
    #26395572 - 12/22/19 12:42 PM (4 years, 1 month ago)

You mean the other post you commented on? That's a few random questions about how cube mycelium should look and transfers.

These pictures are completely different, with new spores and new agar recipe.. Some second opinions would be much appreciated :smile:


--------------------


Extras: Filter Print Post Top
Offlinenarmaduke
Stranger


Registered: 08/23/17
Posts: 197
Last seen: 1 year, 16 days
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: Kmacmo]
    #26395653 - 12/22/19 01:47 PM (4 years, 1 month ago)

just transfer from a leading edge, you’re not gonna have good sections to choose from since the dish is straight from spores. more transfers = more isolation.


Extras: Filter Print Post Top
OfflineKmacmo
The aborted pin
Other


Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 8 hours, 15 minutes
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: narmaduke]
    #26396601 - 12/23/19 12:47 AM (4 years, 1 month ago)

I've got multiple transfers that all look bad, will try a few from these plates but then just trying brand new spores will be best option I think :smile:


--------------------


Extras: Filter Print Post Top
Offlinenarmaduke
Stranger


Registered: 08/23/17
Posts: 197
Last seen: 1 year, 16 days
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: Kmacmo]
    #26398529 - 12/24/19 02:36 AM (4 years, 1 month ago)

Quote:

Kmacmo said:
I've got multiple transfers that all look bad, will try a few from these plates but then just trying brand new spores will be best option I think :smile:




these pics all look like mycelium to me. the bottom pick with the red agar look like it has some green tinge, might be the lighting though. if so i’d just transfer


Extras: Filter Print Post Top
Invisiblehummingbird

Registered: 06/29/14
Posts: 2,134
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: narmaduke]
    #26398543 - 12/24/19 02:55 AM (4 years, 1 month ago)

Those definitely look like cube myc to me. Might as well make some transfers if you got germination. If those were my plates, I would take the rhizo looking parts at about 1 o'clock on pics 4 and 5.

I know some people have good luck with cottony sectors, but I generally go for the rhizo sectors. The leading edge is a good way to go either way. Try not to grab ones too close to the edge, because that's where bacteria usually likes to show up.


Extras: Filter Print Post Top
OfflineeLeSDenes
Mycelium Expander
Male User Gallery


Folding@home Statistics
Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: hummingbird]
    #26398553 - 12/24/19 03:11 AM (4 years, 1 month ago)

Next time try to use less spores, you are using way too much.
I usually just flame the inoculation loop or whatever I am using (scalpel or other metal thing, you can use a nail if you wanted), cool down in the agar, then dip the end of the inoculation loop in the spores and swipe on the agar plate. When I say dip, it means that you just barely touch the spores, you only need just a few spores (not visible with your eyes) as more spores will carry more contamination and you will also have lots of strains crowding on each other making any isolation attempt harder.


Extras: Filter Print Post Top
Invisiblehummingbird

Registered: 06/29/14
Posts: 2,134
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: eLeSDenes]
    #26398571 - 12/24/19 03:40 AM (4 years, 1 month ago)

^Good advice. I've been guilty of doing that too before.


Extras: Filter Print Post Top
OfflineKmacmo
The aborted pin
Other


Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 8 hours, 15 minutes
Re: Cubensis spores germinating on agar(pics), need opinions good or bad? [Re: hummingbird]
    #26398935 - 12/24/19 09:57 AM (4 years, 1 month ago)

Ah good to hear it looks like mycelium :smile: the green tinge is the food color being used up I think happened on all the red plates, but not using food color anymore and changed recipe to 7g agar/7g LME per 500ml for the germination plates so see how that goes. (was 10g/10g 500ml)

I see that rhizo lookin part you mentioned plate 5 1oclock will transfer and pray :P

I'll also try less spores makes sense what you said :laugh:

Thanks a lot for the info guys


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Mushroom-Hut Mono Tub Substrate   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* spores germination on cardboard (pics) comario2 7,951 3 10/12/17 04:29 PM
by AK49
* cubensis spores uk hormoaning 4,281 3 07/26/02 03:27 PM
by Anno
* Spores on agar question .More info plz tripndicular 4,345 5 09/13/02 05:27 PM
by tripndicular
* About spore germination... lemming 3,007 3 11/05/02 02:23 PM
by tripndicular
* spore germination Anonymous 1,760 1 10/08/02 02:04 PM
by Skikid16
* Will my year old spores germinate? case 2,625 2 08/16/01 02:49 PM
by shellacct
* can spores germinate in water? XOCHI 2,310 2 06/26/02 05:53 AM
by Mycelium
* Re: Who are some of the better suppliers of Psilocybe Cubensis spore syringes? Anonymous 1,142 3 07/07/00 04:43 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,887 topic views. 19 members, 224 guests and 57 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.008 seconds on 14 queries.