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InvisibleRRedBBeard
SE Connecticut
Male
Registered: 05/28/12
Posts: 385
Loc: SE CT
Extracting noc from a sterile grow bag?
    #26395058 - 12/22/19 07:07 AM (4 years, 1 month ago)

Was this a good idea or not?
I was given 2 indoor mushroom grow kits, consisting of 2 fully colonized sawdust blocks, shiitake  and chestnut,  in their sealed cultivation bags. Since I have sterilized liquid culture medium and sterile needles and syringes, I decided to try to sneak a little culture out of each bag in order to expand them in my own liquid culture jars.
The only significant downside risk was getting tam in the LC jars, but risk to the sealed bag cultures seemed extremely low.
The sterile procedure involved injecting 2 cc's of prepared, sterile LC medium close to the bottom of the plastic grow bag, sucking it right back up, then injecting it immediately into the prepared LC jars.
I'll make agar plates to test the mycelium next week.


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OfflineDigitalRhizae
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Registered: 06/03/17
Posts: 352
Last seen: 1 year, 8 months
Re: Extracting noc from a sterile grow bag? [Re: RRedBBeard]
    #26396504 - 12/22/19 10:30 PM (4 years, 1 month ago)

Apart from the potential to introduce contamination to either the sawdust blocks or the liquid culture theres nothing wrong doing this. The only other thing that might cause an issue is how runned the culture has been, theres potential for senescen but apparently the worry of senescence is greatly exaggerated.


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InvisibleRRedBBeard
SE Connecticut
Male
Registered: 05/28/12
Posts: 385
Loc: SE CT
Re: Extracting noc from a sterile grow bag? [Re: DigitalRhizae]
    #26397053 - 12/23/19 09:21 AM (4 years, 1 month ago)

Since these blocks are sealed and on the brink of fruiting, the risk seemed very low. Now I'm looking for good shiitake sub recipes that aren't so exotic that I have to run all over creation to find them. LOL


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