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OfflineSvetaketu
The Devil's Avocado πŸ₯‘
Male


Registered: 10/08/15
Posts: 1,508
Loc: United States
Last seen: 20 hours, 41 minutes
Re: Let's All Grow Mushrooms 2020 [Re: Hobbit GDF] * 2
    #26393321 - 12/21/19 12:01 AM (4 years, 1 month ago)

Let's All Grow Mushrooms 2020


Species: P. Cubensis & P. Natalensis

Cubesis Varieties: Avery's Albino, Colombian Rust Spore, & Hillbilly
Natalensis Variety: Super Strength South African

1/1/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Streaked 3 plates each of Avery's Albino, CRS, & Hillbilly, and 2 plates of Natalensis. Poured enough plates to prep for the end of the world yesterday;


Agar Recipe;
11.5g Agar
9g LME
500ml Water
(per bottle)

Decided I was too inebriated to stay up and streak at midnight, so plates were streaked about 8:00AM.


1/4/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



Ayy, germination! at least 1 plate from each variety shows the faintest bit of myc. I could transfer out some isolated colonies, but I'd rather wait until I can identify organized growth, and pick an attractive/fast strain rather than guessing.  My plates appear clean, so no rush to transfer away.

Nothin really worth photographing yet, ill update with pics when there is enough to see :super:


1/6/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Finally got around to making some transfers and taking some pics;

Almost all 11 plates show myc, but these 4 look the healthiest/fastest to me.


took 2 transfers from each plate. Grabbed 1 more Avery plate and took a transfer off it as well.



1/9/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Took some extra T1's from the germ plates, sorry for no picture. Also tossed some bacterial germ plates;



1/14/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


The T1's I took on 1/9 are looking good, but need a a little more time;


a few contams popped up on the original (1/6) T1's, so I took all the dirty ones to T2. Also took T2's from a couple Hillbilly & CRS plates that I'm not satisfied with yet.



1/15/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Okay, so 4 of the original (1/6) T1's are look clean enough for me to go for it. Just took these 4 to grain jars;


Also took T2 transfers from each plate after noccing the jars.
Testing out some pint jars because I was low on quarts :lol:


1/26/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Jars are moving slowly but surely. so far this one is the furthest along;


I shook the pint jars, but not the quarts yet. Also took some T2's and T3's;
 

Tossed a lot of the T1 and T2 plates that I didn't like the look of. Starting to narrow down my options a bit.

Finally got some promising Hillbilly plates, will be throwing those to grain within the next few days;


1/29/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Dropped these to grain;



That's the Hillbilly, so all 4 of my varieties are on grain :rockon:

2/8/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


My original quarts are moving sloooww... This is at nearly a month;

Thankfully some of the pints are moving a little faster, so I shook them and the quarts today.

getting impatient, so I threw a few more cultures to grain;



CRS, Hillbilly, Averys's Albino, and an ATL#7 culture I've been working on since before the 1st.



Thew nearly an entire plate into each jar, and also made up some of these 4oz mini jars I found just for kicks :lol:
Put about 1/2 plate into some of the little ones too, hoping I can G2G them in a few days.


2/23/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



Missed some updates... hope this still all makes sense :lol:

A few of the 1/15 pints finally colonized by 2/15, so they were G2G'd to 4 1/2 gallon jars. 8 days later, all of them looking as far along as this one;

 
(CRS)

However 2 of them have trich;
 

The plates that led to trich were these;
I suppose looking back, I should have known those weren't clean :lol:
Live and learn.


The Quarts from 2/8 are looking good;


from left to right Hillbilly, Hillbilly, Avery's, and CRS. Shook them all today.

The original 1/15 quarts more or less shit the bed... started tossing them, not nearly enough progress for over a month;

(Avery's)

The CRS is a little further along, but still pretty pathetic considering the timeline.
I was experimenting with grain moisture content around then, Looks like I prepped the grain way too dry.

The only jar from that batch that finished in a reasonable amount of time was the Natalensis.
that quart finished colonizing a few days ago, so I spawned it to a shoebox today;



3/10/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Finally made some time to update this log, been a busy couple weeks.

So my P. Natalensis shoebox is looking WACK!



Still smells like mushroom, the look kind of reminds me of Reishi. Not sure if its gonna just keep thickening up into a brick (like reishi would) or if it'll just start pinning on top of that?
Definitely starting to believe this is a totally different species and not just a mislabeled cubensis variety.

Spawning another half gallon of Natalensis today, gotta hurry this one is already trying to thicken up in the jar;

Forgot to update, but I spawned a quart of hillbilly on 3/5, its looking great for 5 days;

Also spawning a 2nd quart of hillbilly today;

I tossed the 1/2 gallon of CRS mentioned earlier, it developed trich as well. thats 3 out of 4 of the 1/2 gallons that went green. Not sure if there is something wrong with the lids (different lids than the quarts) or if I just suck at g2g with them because they are so tall.

Turning into one hell of a grow log, I've had more dirty plates and jars since January than I think I had all last year :lol:


5/12/2020

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Well, time for a small photo dump. Better late than never :lol:

Hillbilly:  

CRS:  

The Natalensis completely shit the bed :lol: not sure exactly what went wrong, they didn't seem happy with their conditions:


Edited by Svetaketu (01/18/21 11:24 AM)


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Offlinecurious.psychonaut
Stranger
I'm a teapot User Gallery


Registered: 10/17/19
Posts: 282
Last seen: 4 years, 9 days
Re: Let's All Grow Mushrooms 2020 [Re: Svetaketu] * 3
    #26393444 - 12/21/19 03:13 AM (4 years, 1 month ago)

:mushroomtwirl: Jan 1: Spores to Agar :mushroomtwirl:


Here is our parent shroom (P. cubensis var. B+, 39g) and the plates it came from: from spore plate (IB6 from Oct 15) to T3 plate (E2ivb from Nov 6) that was used to noc the jars.



Now let's transfer some spores to agar. I use a noc loop, a blowtorch, a SAB, and an alcohol jar to keep/dip instruments in while working (optional). My agar recipe is 9g LME, 9g agar agar, 450ml water. I found Bod's Agar TEK and spore transfer video very useful when I started.

What's that clump of foil? It's the print! I had thrown it away while cleaning my work area. Only a few hours later did it occur to me that I had a print drying in the SAB, so I salvaged it from the garbage. I hope it's all right. I also didn't spray the SAB on the inside and didn't wrap the petri dishes with anything. I trust in gravity (a.k.a. I'm lazy).



:mushroomtwirl: Jan 4: Visible germination :mushroomtwirl:


And we have liftoff! 5 out 5 plates germinated! The very first visible spots were on day 3, actually, but I thought they'd be difficult to capture with my camera. Let's have a look at dish 2:



See those lovely white tufts on the streak line? Their location means they're most likely cube myc (or contams picked up from the print, but let's hope not). The black stuff is either spores, soot from the flamed loop, or maybe some contam (though very unlikely, since molds would not sporulate/acquire color so quickly and bacteria doesn't really look that way)... The colonies are still much too tiny to transfer.

:mushroomtwirl: Jan 9: Transfer, the First :mushroomtwirl:


A few days ago would have been better, but I just finished the first transfers. 15 dishes from the 5 masters. Why so many? Judging from my first culture attempt, T1 is the most critical for selecting the "right" type of myc, so I want to have options in what I culture further. What is "right"? I don't think we know for sure, but I'm looking for fast-growing, rhizomorphic myc (per classic recommendation) as that's what's worked best for me so far.



Edited by curious.psychonaut (01/09/20 06:19 AM)


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OfflineMooseMomma
Crazy Old Pitbull Lady
Female User Gallery

Registered: 12/20/19
Posts: 18
Loc: Coastal Carolina
Last seen: 2 years, 7 months
Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] * 3
    #26393764 - 12/21/19 09:32 AM (4 years, 1 month ago)

I’m super new at this, just started about a month ago, but I’m looking forward to this challenge.


--------------------
The people who say it cannot be done should stay out of the way of those who are doing it.


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Let's All Grow Mushrooms 2020 [Re: MooseMomma] * 2
    #26393822 - 12/21/19 10:31 AM (4 years, 1 month ago)

In this:

Psilocybe cubensis var. Purple Mystic

01/01/20: Streak Day




01/10/20: first transfer


Took a slight temp increase to coax the spores to do their thing: just hoping its purple Mystic spores lol

01/15/20: Second transfer



T2 going down in the morning, not sure what the gap at 7:30 is about


Tribe Grow Along 2020-Caps' Log




Edited by Caps McGee (01/15/20 10:47 PM)


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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
Trusted Cultivator
Re: Let's All Grow Mushrooms 2020 [Re: Caps McGee] * 3
    #26393895 - 12/21/19 11:36 AM (4 years, 1 month ago)

I'm in. This is going to be epic. Not sure what i'll do yet but I'll do something. Probably a cube. Unless i find some interesting spores to try before then.

 


Edited by verum subsequentis (01/02/20 07:11 PM)


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OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,906
Loc: Washington Flag
Last seen: 7 months, 22 days
Re: Let's All Grow Mushrooms 2020 [Re: verum subsequentis] * 2
    #26393942 - 12/21/19 12:12 PM (4 years, 1 month ago)

I'm up for a game!

Spot reserved

DNDRND GROWLOG


Species - panouleus cyanescens, psilocybe mexicana, Psilocybe Cubensis, Psilocybe cyanescens, Psilocybe medullosa (ran out of spores so subbed for P. Cubensis var "blue meanie"

Variety - Jamaica (pan cyan), Jalisco (mexicana), PE6 (cubensis), south Africa (Psi. Cyan), Blue Meanies (cube)

Spores streaked to agar - January 1st 2020

Agar was prepped in "no pour" style, WBS grain soak water at a 2% nutrient concentration (98 grams water/2 grams WBS soak water) spores streaked via inoculation loop inside SAB.


Update - swabbed Pan Cambo var sandoz, pan Bisporus, and RW to 3% nutrient PDYA mix in no pours on 1/12/2020


Edited by DnDRnD (01/12/20 08:34 PM)


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InvisibleYesum
Furry as Fuc
Male User Gallery


Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: Let's All Grow Mushrooms 2020 [Re: DnDRnD] * 3
    #26394111 - 12/21/19 02:28 PM (4 years, 1 month ago)

I'm in.
Species: Cubensis
variety: CRS, and PEU
*PEU was replaced with the Gatz

Cool idea :super:

Counting down the hours.

Streaking at midnight. :growshrooms:

Hear we go


Inoculated PEU and CRS again on 1/11. No germination on the first inoculations. also decided to streak spores from a Gatz print. Just in case the syringes don't work again


Update: 1/20/20

The PEU still has no germination. Giving up.

The CRS is on its first transfer


The Gatz has germination already. Will be doing t.1 transfer later today


Edited by Yesum (01/20/20 02:40 AM)


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OfflineJohnnyKarate
So Fresh 'n' So Green
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Registered: 10/16/19
Posts: 113
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Re: Let's All Grow Mushrooms 2020 [Re: Yesum] * 2
    #26394133 - 12/21/19 02:41 PM (4 years, 1 month ago)

:mushdance:Let's Go Grow 2020 - The Johnny Karate Way!:mushdance:




3 Cubensis Varieties

Rusty Whyte
Great White Monster
Mystery Print



January 1, 2020 - Print to Agar

I streaked 1 Pasty for each print with a sterile swab I made myself, streaked roughly 7:00am central time. The dogs were so kind as to wake me up at 5:30 for breakfast, so I took that opportunity to get the SAB setup and get to streaking plates with my spore prints. Now I'm just waiting and sending some positive 2020 New Year vibes their way!


Inoculation/Streaking Technique:
 
Nothing fancy here, I get the print opened up since they're typically wrapped up pretty good around the edges if you're using tin foil. I ready the print and leave the foil tented and bent over the print so it's shielded from the bad guys. At this point I take the homemade sterile swabs I've previously prepped in the PC which are wrapped in foil and pop one of the ends through the foil. I carefully wipe it on the clean plate to saturate the swab which I find helps pull up spores from the print. I then gently apply the swab to the edge of the print and slightly twist and pull from center outward to grab just a small amount of spores. I immediately move to the pasty, slightly crack the plate on one side and do my best Zorro impression and zigzag swipe the plate.

Pastys were made using the following LME recipe: 400ml water, 8g agar & 6g LME. I microwaved until boiling, shook well and added blue and red dye so my mushrooms can be treated like royalty sitting on their little purple thrones! I let cool for a bit, poured into mini rounds, covered hole on top of lids with micro-pore tape, put a small square of paper towel on the top and finally covered with foil and into the PC for a 30 minute 15psi bath. Let them cool over night, these were made last weekend in preparation for this group grow.



January 5, 2020 - 1st Transfers
 
Have some vigorous mycelium growth showing up on all three plates I streaked on 1/1.

The GWM streak plate turned out rather pretty, you can see exactly where the swab was streaked to the plate and the growth looks rather healthy.

Great White Monster



The Mystery print is showing some explosive growth, not sure if this is because the print is newer than the rest since I made it just a few days prior to this grow. You can't see the streak pattern as cleanly at this point, but 1 1/2 days earlier it was much easier to see.

Mystery Variety



The RW plate is the saddest looking of the 3 and I also didn't get a good smooth swipe on this one, my swab stuck and bounced across the surface a little which also caused me to lose my grip and almost drop the plate. I believe these quick movements caused some air currents and resulted in some slight contamination. The thick white growth showed up just a day after streaking while nothing else was present. I don't believe it to be mycelium and I took my two transfers from the other end of the plate where you can see the fainter mycelium germinating.

Rusty Whyte



I took two transfers from each of my three different streak plates to make sure I can get some healthy plates after just a few transfers and get this show on the road. The transfer pieces are on the smaller size,about the size of half a grain of rice and there isn't anything exciting going on at this point so I didn't think it warranted a picture through the sides of my moist Pastys. I label mine with X1A & X1B to represent 1st transfer A & B, so after I transfer again if I take two from each plate again I'll be looking at X2A1(2nd Xfer of A, then 1 of 2) X2A2 X2B1 X2B2, maybe not the most straight forward, but it makes sense to me so that's all that matters :smile:
X1 Pastys



January 11, 2020 - 2nd Transfers :snow:

With work being super hectic and having very limited time to do anything I finally got around to making some more Pastys for these transfers late Thursday night and was able to have everything ready to go for the X2s on this lovely Saturday morning.
Just about the same routine as the 1st round of transfers, the dogs so lovingly woke me up at about 6am so I took that opportunity to get the furnace fan shut down and get the SAB prep'd and ready to rock 'n' roll :rockon:

I haven't had time to keep an eye on the growth or progress of the 1st transfers so it was basically an unveiling when I popped them into the SAB and snapped these pictures. To my surprise all of them I think are looking incredibly clean, quite strong and healthy. The weakest I would say is the 1st one you'll be seeing, which I don't think I will be putting to grain, just pulling a transfer from it and pitching it to free up another mini round. Since the rest are looking surprisingly good I am going to prep 6qts of oats today and drop the last 5 Pastys as well as a fine ass looking RW dish I have taking off from a recent grow that I cloned (1st time cloning and it is going great, but more on that another time).

My initial plan going into this grow was to transfer 2-3 times before I even considered going to grain, and probably taking it out to 5 transfers for the hell of it. I'm still probably going to take it out to X5, but since these look as good as most of my 3, 4 or 5th transfers I've done in the past I figured why not.
I'm taking this opportunity to test my sterility and see if my technique will allow for me to get away with only doing 1-2 transfers in the future, so I can cut down on my time from germination to harvest. I'm not so much focused or concerned with rushing or speeding up the individual steps as I am just streamlining my process.

Great White Monster - X1A    Great White Monster - X1B
   
1st is the weakest and his journey is ending here aside from a transfer taken. 2nd isn't the strongest of the bunch, but I'm going to roll with it anyways and drop him to grain with the rest of the X1s. As you'll see a few of the plates had a drop of water land on the mycelium off the lid, I've had this happen in the past and haven't had issues as long as I'm not tilting and spinning the shit out of the plates. The condensation is also less than desireable, but it hasn't adversely affect my growth or progress so I just deal with it and have limited sight through the sides of the plates.

Mystery Variety - X1A    Mystery Variety - X1B
   
Not much to say about these other than I'm loving the symmetry and cleanliness of these plates, by the end of the day they'll be acquainted with their future grain feast!

Rusty Whyte - X1A    Rusty Whyte - X1B
   
Same for these plates, nothing too crazy to say or point out other than the fact that these X1s are probably my cleanest and healthiest X1s to date. Everything I've had since I've started this hobby has been tomentose growth on agar, probably since I'm using the same agar recipe and varieties, but once I drop them to grain and/or spawn them to bulk with coco and verm I usually start to see rhizomorphic growth at that stage. In my limited experience though, the end result has been as good as anything else.

X2 Pastys
Here's the end product after taking a single transfer from each of my 6 X1s this morning. With my work schedule not due to change for a few months my next opportunity to make transfers will most likely be a week from now, until then...


January 19, 2020 - 1st Transfers

Same shit different week, crazy busy with work so I am more or less just getting these pictures up to stay on top of that and this helps keep things a little more organized as shit gets hectic. I was limited on how many plates I had prep'd so I only made a single transfer from each plate. After I took a transfer from each I tiger dropped all of these to grain, so I have 5(IIRC) 2nd transfers that I dropped to grain as well as these 6, for a total of 11 qt jars colonizing away getting ready to meet their shoebox fruiting home.

The plates that were dropped to grain a week ago were ranging from 35-75% colonized tonight, January 22nd. I gave them all a good shaking and we'll see where they end up when I get another chance to get my myco on this upcoming weekend.

This round of transfers were not put to Pastys like everything in this log so far, but rather into some 100mm petris, of which I just got 500. I'm pretty excited to get back to petri dishes after that's what I originally started with and then went on to dabble with Pastys.

Great White Monster - X2A    Great White Monster - X2B

   


Mystery Variety - X1A    Mystery Variety - X1B

   


Rusty Whyte - X1A    Rusty Whyte - X1B

   




Edited by JohnnyKarate (01/22/20 09:32 PM)


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OfflinePopaCap
Convicted mycophile
 User Gallery

Registered: 07/23/18
Posts: 458
Last seen: 7 months, 16 days
Re: Let's All Grow Mushrooms 2020 [Re: JohnnyKarate] * 2
    #26394382 - 12/21/19 05:16 PM (4 years, 1 month ago)

Kick-off 1/1/2020:

Today I went streaking. 3 plates each of ESS and Tasmanian. What a great start to a new decade:rockon:


1/8/2020

Made first transfers. 4 transfer plates for both ESS and Taz.


I’ll probably take a fifth transfer from this Taz plate, as I really like the growth I’m seeing in the area right under β€œTaz”. It was late to germinate, but has blown past all my other germ plates in the last 2 days.



--------------------


Edited by PopaCap (01/11/20 12:31 PM)


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OfflineInthepit
Aum Mani Padme Hum
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Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] * 2
    #26394666 - 12/21/19 09:20 PM (4 years, 1 month ago)

:amanita2: :mushroom2::sporedrop: :amanita2: :mushroom2: :cubie::mushdance::mushdance::sporedrop: :amanita2: :mushroom2: :cubie::amanita2: :mushroom2:
In The Pit's LAGM 2020 GROW LOG

Species: P. Cubensis
Variety:  PESA, Pacific Exotic Spora Amazonian
              InThePit's 2020 LAGM Log CONTINUED in my journal
              TOC,  Texas Orange Cap
TOC & RW Grow Log

I'm in! With a tribe giveaway print.

2 Jan 2020:
It's so great to see all this! And really interesting seeing all the different setups!  :thumbup:

My Group Grow Plan:

1. SAB, air temp here is a constant 81-85, 12 hrs natural light, 76% humidity. Good surf!
2. LME c10's Agar Guide
10gA, 7.5gLME, 500mL Oat broth, food color. 40 min PC.
Cool agar to 45 C/113 F. Use something to elevate work area for the plates. Stack plates, put sleeve back on. Let cool in SAB for a long time.
3. PESA print from The Tribe Mass Giveaway. Innoc loop to 3 100mm vented plates. And Glad wrap.
4. Grow till good leading edge. Take a wedge from just outside of visible leading edge.
5. T1 transfer a small wedge from each of the 3 plates to another 3 plates. Repeat (TX) till awesome!
6. Oat prep. 2.75 qrt jars of oats for 7. Boil till grey inside, lay out to dry. (polyfill, no foil). Fill qrts 75%, PC 120 minutes.
6a. Freeze 500ml grain water containers for next agar sesh.
7. Half of the plate, multiple wedges, to each Qrt Oats.
8. Roll wedges around, shake at 30% colonized. Wait till fully colonized.
9. Shoeboxes, 2 quarts each, Coco Coir to 4", mix, cover w/coir. (not as wet as last time!). Snap cover on till fully colonized. Making Shoeboxes
10. Crack open cover for fruiting
11. Clone a couple good ones. RR picks a clone, Masters and Slaves Hunting Clones
12. Nesco dehydrator, Mushboy Tea!  (ginger tea), Gypsycurse Gummies!

6 Jan 2020:
Got a flight booked to get back home, can't wait to pour plates!
Kinda funny, I'm enjoying figuring out this BBCode. If I were home this grow log would show actual growing stuff instead of me dicking with the format! :facepalm3:

17 Jan 2020:
8 hours, 4,000 miles, 35,000 miles high, finally home!

I'm a little worried about the tiny potato bits in the agar?

But here we go at long last. IMHO getting this late a start has only helped. But just in time as the topic is shifting from agar to transfers!
....

4g agar, 7g potato, 50% Oat broth, 400ml.
Green food color cause....shrooms!

GO NINERS!

18 Jan 2020:
Oh I'm exhausted! That's hard work. The loop is really flimsy. Pretty exciting though and there was a surprise print. Fk included TOC! Thankyou!
...

So far no condensation, poured at 117 F, stacked on two old plates, covered, in the SAB, inoculated the next day. Air temp here mostly 83 F.
The other plates, GT, sat in the fridge, cold and neglected while we partied in France, ha! I even dumped water out and wiped the lid with a paper towel.
GO NINERS!

24 Jan 2020:

Wow so much contam mayhem, very interesting.
:mushdance::sporedrop: :amanita2: :mushroom2: :cubie::mushdance: :doge:

So...is it time to take some T1s?
1 and 4 PESA looking good! 6 days!  :chief:  The Tribe Mass Giveaway
Going for PESA T1.1 & 1.2!

2 and 5 TOC what's that brown stuff in the middle??
Samskara92 said:
Definitely pigment being eaten. My TOC cultures always eat the green pigment out of my plates.
Looks like I should grab a little T1 before contam takes over?


.....

3 and 6 GT T3 from my BIOHAZARD.

So...7g potato / 350ml = 4% nutrients, right? 350ml cause I forgot to get it back up to 400ml.
So that's 4g agar, 7g potato, 50% oat broth.

When the music is your special friend, dance on fire it intends...
GO NINERS!

26 Jan 2020:

So all in T1, TOC & PESA , GT is from earlier.
I think TOC & PESA really like this batch of agar.
4g agar, 7g potato, 50% Oat broth, 400mL 

........

Looking good, IMHO, from 1/18 start.
Super Bowl Sunday! GO NINERS!  :facepalm3:

29 Jan 2020:

Plate 4.2 I have hopes for some pinning action someday!
       
My PESA looks fluffy too!  :rockon:
            Wow over night (unless you look super close) something jumped in the T1.
             
30 Jan 2020:

That's just one night's difference.
What should I do?
  • 1. Leave it
    2. Take a piece of each and see what happens?  :shrug:

Edit: Aha! Once I pulled the plates out of shoeboxes they picked up the pace, including some plates with contam already!
 
31 Jan 2020:

Hobbit GDF said:
I'm having alot of trouble with my plates. The myc growth is so thin and almost invisible. It's stays real thin while it colonized.
I think it's from nutes being so high. I've changed the recipe a few times and trying to do 9g agar 7-6g brf.
Potato flakes-and honey was my old one and they grew fine on that.
I've used grain soak and would that be why? I didn't cut it with water. Just agar and soak.
Should I go back to plates T1 or 2 or just transfer out of the T4 AND UP?
...full time job, wife, 8 kids, 2 dogs, 2 cats, squirrel, hampster. OMG! Only had two myself. LOL
So I'm new here but you can see the recipe I used, which has similar ingredients, but diff proportions.
4g agar, 7g potato, 50% Oat broth, 400mL. From Sagari.
And the myc loves the green food color: WATER, PROPYLENE GLYCOL, FD&C YELLOW 5, FD&C BLUE 1, AND PROPYLPARABEN,
This product has no significant nutritional value. -McCormick
       
Also, I think I've read going past T3/4 not so great.
footpath said:
Going past T3/4 is fine for the most part. T8 is probably the furthest I've taken one and I still didn't notice any loss in performance.
Sometimes you can't even get the culture clean in 3 or 4 transfers.
      Oops  :shrug:

Holy Mackerel Batman!
                  :doge:
Look at that mold go!
1 Feb 2020:

Inthepit said:
The two GTs look so different.
  1. Should I send both to jars?
  2. Go for T4 on each?
  3. Is one clearly contam?
  Pic #1          Pic #2

:shrug:
Inthepit said:
mushboy said:
:tinfoil:
So took T4s and...wiped inside the T3 lids!
So mebbe there's a difference here...
     

:tinfoil:  LOL
4 Feb 2020:
I think it's time for PESA to jar!
   

Oat prep:
For 4 jars, 1.8 jars oats, in the 7 Liter pot boil 5 Qrts water. Go 35 min and start checking for grey inside the oat. Drain and let almost dry.
Started drying 0900. Almost dry 1400.
         

Save the liver! Uh, broth...250 mL for each 500mL agar.
   

PDA: 4g agar, 7g potato, 50% broth, next time Blue food color!
Oh, and I strained the potato bits out! I don't think they're supposed to be there. 2 hrs to cool 117F.
And this time 500mL    the blue looks green.
11 Feb 2020:
Inthepit said:
Whoo hoo! We have pins in plates!
         
12 Feb 2020:


So is it time to shake, rattle and roll?
Or am I IMPATIENT! :popcorn:
13 Feb 2020:
edit: OMG! all my germ plates are going off!
 

should I:
  1. Put them in the fridge
  2. take more cuttings
  3. just watch and laugh, I have plenty going now...


13 Feb 2020:
So I'm going to follow this Coir Tek from Shaper Dreaming.

1) Take 550g of coir. For 5 shoeboxes
2) Boil 2.5L of water +1 cup
3) Put 2 cups of boiling water into a cooler for 10 min to pre-heat the cooler
4) Dump out the water
5) Add the coir the cooler, then pour water over it
6) Close lid, wait 60 min
7) After an hour stir up the coir to try make sure you don't get dry spots
8) Wait until cool (I just wait overnight)

And...since the last recipe:
"650g of coir that's 3250. which is 3.25L of water.-Bod",
was way too much water for the Coir I use.
        :doge:
I'll try 25% less water. That's Walmart's name on the package.
It would be interesting if anyone else uses this brand and has to use less water?

16 Feb 2020:
PESA and TOC are pinning, one even dropped spores!
But GT, older, isn't doing a/g. Am I just isolating contam?
         
GT seems to be just fuzzy...:shrug:
It's weird how I can't get my phone to focus on the myc, I even took the cover off!
Actually, that pin counts as the first shroom I've grown in 45 years!
(No replies, I'll just shoot from the hip.)

18 Feb 2020:
Glad you're back too!

c10h12n2o said:
Happy to help :smile:
...cleaning contams. I've gotten an isolate in 3 transfers like this (!)
Does this mean, T1 thru T3? And if so does that mean you are using the same streaking technique with myc?

And maybe I can sneak this question in, no one seems to want to touch it.
My GT isolates keep looking "just fuzzy", am I just isolating contam?
           
    and later that day...
Thank you for such a detailed response!

using a 15C scalpel blade
Awesome scalpel, when I actually grow something, I'll be spending on equipment.

About your question:
Have you ever grown it out?

Sorry I don't know what that means. My guess, what I was thinking, is to just put some in a jar and let 'er rip! LOL Then if it goes 100% colonized, G2G it out.

They definitely look weird.. what kind of agar recipe are you using? Do all your plates look like these or just these cultures?
Well I started with the SAGARI philosophy, but it seems like the agar is really wet. 4g agar, 7g potato, 50% oat broth, for 500mL. Next batch mebbe 8g agar, 7g potato...Oh and I strain the potato bits out because they're distracting. Can hot agar go thru a coffee filter? Oh and some food color which the PESA loves to eat.

I cant tell from the pics, does it look powdery at all? The first and 2nd pics kinda do but last one not so much. I've had lots of pan cyan myc look like that 3rd pic
No not powder, more like cotton ball.
I know this GT refuses to go rhizo. The PESA and TOC with the same agar are fine.
            That T1 went to Oats ACKCHUALLY!
PESA has gone to Oats and is about 80% col and lookin goooood!
TOC is slower and mebbe soon to Oats. Since Oats are so controversial here's what I'm using.

Some myc just refuses to go rhizo on agar, but I'd be surprised to see that across that many cultures . How many transfers in are you from the original print/clone?
It looks like I'm at T5 and needing to go T6. My labeling system matured as the plates evolved.
   
Funny thing, this pin is my first mushroom in 45 years! OMG!
   

So to anticipate your answers:
  1. Ya, jar that bitch!
  2. No, dummy, you can't get hot agar to go thru a coffee filter. Filter the crappy potato first. Yes coffee filters work, cheesecloth bettah!
  3. Ya SAGARI is for a certain way, just stick with 8 to 10g agar, and the broth is ok.

18 Feb 2020:
Inthepit said:
natedawgnow said:
All of those jars look extra fucky. Bacteria for sure.

As for wheat, just go to a feed store and ask for whole wheat and check out my
prep tek for info on easy prep.

Thankyou!
All of them?? Dang, well:
  1. It was just T1
  2. The Oats were dryer, but not 24 hr dry.

Guess I need to learn the different types of white stuff...

I can hear GypsyCurse  now!
GypsyCurse said:
Oats blow goats
:feelsgoatman:

Inthepit said:
natedawgnow said:
Plus there's a ton of moisture in those jars, wet grains, etc
Ohhh! There ya go, they don't look that wet and they didn't feel that damp.
So my calibration is off, I  need to think dryer!
They just don't look good. Could try fruiting the few that don't have obvious
green in separate shoeboxes or spawn bags

I'll try that!
:nicetry:

21 Feb 2020:
Inthepit said:
A.k.a said:
Inthepit- my gt plates for lagm looked a lot like that at first but definitely cleaned up by t4. Although I did put it on plates with a recipe I get good rhizo from.

Thankyou and that recipie would be...drum roll...

My Rx is: 4g agar, 7g potato, 50% Oat broth, food color, for 500mL
:hatsoff:
Oh, and I microwaved agar from the fridge and the potato looked crappy. Not expecting mush out of this batch. However I used c10's method of pouring a little agar and swirling the plate to cover. Kinda cool...:cool:
A.k.a said:
lol I use like 6glme and 7-8g agar to 450ml.

Then I pour plates on the thin side. I’ve been doing all kinds of recipes to see what happens and  that’s been my go to, although I did just have a super fluffy aa+ culture go nice flat rhizo on t4 with thicker plates mixed like 5g lme to 10 agar.


Aa+ and gt were by far the fluffiest for me starting from spores. Just a big thick mess almost up to the lid until t3.

I gotta get some lme, can't find a single brewing supply company on this pathetic island.
Gonna go begging at a brewery next.

21 Feb 2020:
LadysKnight said:
Agreed, first is mold.
jbgtaa said:
Inthepit said:
Am I isolating contam? This GT looks so weird.
I'm out to T6 now and no change.
       
:shrug:
First one is def not cube Myc. Second one just looks like MS culture but also could be something else.

Looks I'm gonna have to give up on GT

22 Feb 2020:
Inthepit said:
Quick question, should do transfers from a pin?
Or is a pin isolated enough already?
   
:shrug:  :hatsoff:

Thankyou for your reply!
AyePlus said:
Did the pin come from a clean plate or was it from the germ plate?
Yes the pin came from the Germ plate.

I generally do At least 1 xfer from TIssue Culture or Pin Clone plate and make a grain jar to test from that, and put the TC/PC plate in cold storage, if the transfer grows out clean and the clone is a winner I’ll slant from the TC/PC plate and make a bunch of work plates.

if it was a germ plate I might still do 2-3 transfers.

Imo just based on looks that one needs another 1-2 to be clean, or your temps are swinging pretty drastically.

The room isn't temp controlled, yer right. Temp is 79-84F.

I'll do more Ts, thanks  :hatsoff:
PrimalSoup said:
The temp fluctuations give rise to growth rings sometimes, so since this came from a clean plate and looks very regular aside from those rings you probably don't need to do more transfers. IMHO that is. 

But it wouldn't hurt to put one aside in a place that doesn't have temp swings to see how it grows. :shrug:

AyePlus said:
:whathesaid:
Looks pretty clean to me if your temps are swinging.
I’d make a work plate off that and put it in cold storage, drop the work plate to grain and run a test fruiting, then make a slant off the plate in cold storage and make Another master plate and more work plates if its worth keeping.




26 Feb 2020:
Quote:

Kizzle said:
Quote:

mushboy said:
ive been getting tons of pinmold right about the same time.


its been really fucking frustrating. green of some kind usually follows:oldman:



It's Rhizopus and it's one of the few molds that will start growing on coir. When it's done growing another mold may take it's place, usually Penicillium. It can also be sneaky by colonizing the inside of the grain while the mushroom mycelium colonizes the outside. So when you shake your jars watch for blue or grey discolorations.




26 Feb 2020:
Getting ready for the next try...
SHOE BOXES Sterilite 14x8x4" https://www.shroomery.org/forums/showflat.php/Number/26009662
1) Take 550g of coir. For 5 shoeboxes, break it up
2) Boil 1.875 L (.125+1.75=1.875, on the blender) still too wet 2/27
3)Boil a pot of water and put into a cooler for 10 min to pre-heat the cooler
4) Dump out the water
5) Add the coir the cooler, then pour water over it
6) Close lid, wait 60 min
7) After an hour stir up the coir to try make sure you don't get dry spots
8) Wait until cool (I just wait overnight)
1:1 and a thin layer on top, tamp the edges
Spray Bottle
spawn run is 8-10 days. pins in 10-14 days. harvest in 15-21 days.(best case)

27 Feb 2020:
          uh, oh...


2 March 2020:
.........   
PESA Shoebox 5 days
  1. Oats still too wet, next time overnight drying.
  2. CC still too wet, next time less than 1.5L per 550g coir.
    :hatsoff:

5 March 2020:
For now it'll be sticking with 10g Telephone agar, 7.5g Briess Pilsner LME, 500mL H2Ooooo  LOL!

So what do you think?
This setup, which is hopefully to keep gnats out,
looks like something from the laundromat!
Just walk up, open the lid,
and pull out yer shoebox!   :laugh2:


7 March 2020:
My baby pictures :laugh2:  PESA from Filthyknees!
1/18 spores, 1/24 T1, 2/18 Oats, 2/27 shoeboxes, 3/7 Pins!  :hatsoff:

9 March 2020:
Now the fuzzy base of these pins is an
indication of not enough FAE, correct?

I've just started opening the lid much more and fanning.
So maybe the fuzzy will decrease,  am I right, am I right?

The moisture is less now, so I lightly misted this am.
        PESA
:popcorn:

11 March 2020:
And a new day begins,
and of the dream called yesterday,
no trace.            :sporedrop:
PESA Pacific Exotic Spora Amazonian

13 March 2020:
I want to thank everyone! I couldn't have done it without you.
For this Steppenwolf this is a wonderful community.
So here is the conclusion for PESA spores from Filthy to agar,
isolated to only T1 and got away with it!
And even learned how to print! 40
Around 300g ea box...4 boxes  :hatsoff:

  :amanita2::cubie::cubie::cubie::amanita2:

16 March 2020:

So, second shot at TOC; TOC & RW Grow Log
10g Telephone agar, 7.5g LME, 500mL Oat broth.
Grain broth because Primal's Experimental PE Grain Inoc>Agar Isolation Log (with pics and paragraphs :wink:
Boiled some oats for broth.
PC glass petris wrapped in foil and agar for 40 min.
Taken out hot as soon as the valve dropped and into the SAB.
Wait 2 hrs for agar to cool to 113F and pour baby, pour!
:panic:

Now oat broth is back!
PCing the plates turned out wet and sloppy.
Next time I'll try the oven, not for my head, for plates. :wink:

Going all the way back to spores. Excited to try c10's spore spreading tek.

Fricken Corona, cops won't even let surfers out in the water!
:cop:

19 March 2020:
So I soaked these for 4 hours. It seems there's some debate about soaking or even not soaking.
I really like the writing in this TEK, so I think I must be off somewhere.
(Sorry for double posting)
So I thought 2nd flush would be more impressive. Is this even 2nd flush?
I soaked these for 4 hours. Or mebbe it's too soon?
Just seems as confused as I am...  :wink:
    :amanita2:  3/19 8 days  1st flush 3/11
fahtster said:
Was your first flush a big one?  If 2nd flush comes in pretty quickly after the 1st, it’s going to be from the areas that didn’t fruit the first time... so if you had a big 1st flush, the 2nd isn’t going to be very big and vice versa.  3rd flush should be bigger in that situation

Faht

Thanks fahtster
A.k.a said:
I have a couple doing the same thing.
Had massive first flushes and right now the second flush is like 3-7 mushrooms.

fahtster said:
The second flush is usually just the sub fruiting knots that have already formed in areas that didn’t flush the 1st time, so that part of the sub is already locked and loaded... that’s why the 2nd flush can come in like 3-5 days.. so in order for the sub to produce fruits in areas that already fruited, it has to produce fruits starting from nothing.. so while the 2nd flush is coming in, the sub is starting over in the areas that already fruited, that’s why 3rd flush can be bigger than the second.

Of course this is a general theory.. most of it depends on the culture speed and other factors but that’s a good starting place. 

It really depends on the size of your sub and how much it put out in the 1st flush, as far as your question goes.. if 1st flush was big enough relative to the size of the sub, it could be a 1 and done.. or at least not worth waiting for the later flushes if you need the space

Faht

So here's what I got;
:amanita2: :cubie: :cubie: :amanita2: :feelsgoatman:

290 grams, 14 days from spawning...all the shoeboxes (4) were around 300g
Thanks for all the input, I have a better feel for it now.

21 March 2020:
:mushdance:    Wooo hooo, 2nd flush is bigger...PESA

    :shockedpussy:        :dancingshroom:

Oh shoot, I misspelled Filthyknees!

23 March 2020:
:cubie: :cubie:  :mushdance:  :amanita2: :mushroom2::mushdance::sporedrop: :amanita2: :mushroom2: :cubie:


23 March 2020:
TOC & RW Grow Log
InThePit's 2020 LAGM Log CONTINUED in my journal





Edited by Inthepit (03/28/20 12:57 PM)


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Offlineverum subsequentis
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Trusted Cultivator
Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
    #26394675 - 12/21/19 09:25 PM (4 years, 1 month ago)

what's with the picture?


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OfflineInthepit
Aum Mani Padme Hum
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Re: Let's All Grow Mushrooms 2020 [Re: verum subsequentis]
    #26394683 - 12/21/19 09:33 PM (4 years, 1 month ago)

Edit: Now the picture is from inside the tube of a wave. Surfing In The Pit! :wink:

The start of something great?
I can delete it if you like. It was the end of my biohazrd grow.
My Biohazard!


~~~cbk~~~
Add whatever pics you like. It's your grow log:cheers:


Edited by Inthepit (01/08/20 10:30 PM)


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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] * 1
    #26394810 - 12/21/19 11:37 PM (4 years, 1 month ago)

Species: Cubensis
Var: Cambo, B+, RustyWhyte


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OfflineNikoyo
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Registered: 01/29/17
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Re: Let's All Grow Mushrooms 2020 [Re: fungi4us] * 2
    #26395156 - 12/22/19 08:29 AM (4 years, 1 month ago)


species: p cube, Pan, Tampanensis
Varieties: AA+, atl7,Panaeolus Cyanescens

12/29 pouring some plates to be ready.

waiting for that perfect temp..i ended up getting lazy waiting and poured early so my germ plates have a bit of condensation:shrug:

1/1/20
swabbed spores to agar with sterile swabs. ended up doing 5 plates of aa+, 6 plates of atl7, and 6 plates of pan cyan. I wish I had started using swabs sooner, makes life so easy when dealing with spores..plus who knows were my inoculation loop went:facepalm3:


1/3/20
No signs of germination yet, Ill look again in a few days.

1/5/2020
welp looks like the aa+ and alt7 prints I had were not as clean as I was hoping they were, thankfully I swabbed a few plates so I hope I get something better on the others.



looking like some sort of mold on the aa+


the pan cyan plates are looking clean tho, little bit of germ this morning :smile:


1/9/2020

T1 transfers completed, aa+ in the back, pan cyan up front. unfortunately all my atl7 germ plates were nothing but bac. so ill only be continuing with the aa+ and pan cyan. from reading up on a couple pan teks it was suggested to only go after the wispiest and fastest growing so I took multiple transfers from each plate. Will be comparing them all to see which will be the "best" to continue with for t2 transfers. If all t1 transfers are looking clean in a few days I may put a couple to lc and grain. will be starting to look into poo/straw teks as well over the next few days so I am familiar with them and ready to give them a go.

1/16/2020
T2 transfers done

the aa+ t1 plates were looking nice and clean so ill let them grow out a bit more and get some grain jars prepped for them this weekend if i have time. The pans on the other hand are still rather un uniform so ill be waiting to see how t2 plates go before I start any lc. Was able to get my hands on a cheap impulse sealer and a few spawn bags so ill be able to start prepping some spawn bags for them.

1/18
----
started 24 hr wbs soak before leaving for work

1/19/
-----
-woke up early started straining wbs 1hr
-loaded up quart mason jars 3/4 full, did 8 jars about half full to try out some t1 plates
-pc 90 min at 15psi
took most of the day as I can only fit 10 jars per run, 2 runs of jars and 3 grain spawn bags for other projects. after each cycle, let pc cool before removing jars/bags and starting next run.

1/20
----
picked the 4 best t1 plates of aa+ and pan cyan and put to grain masters. will most likely g2g the aa+ to grain spawn bags for expansion. I plan on using the pan jars for several different options might put a few to wood chips as I had planned on starting a few outdoor patches this year; going to try some spawned to coir, as well as try different substrate options.


1/26/20
----
t3 transfers complete, took the  best looking aa+ and pan cyan t2 plates and inoculated two lme LC's; finally getting a chance to give the stir station I made last week its maiden voyage.

also made a few transfers to grain masters. kinda a lazy update..been a real hectic work week.

2/6/2020
another short update as Ive been way to busy at work to really do any myco work lately, got a few min the other night to do some t4 transfers. shook t1 jars and ill be testing the aa+ lc I made. hopefully be spawning some jars soon :willynilly:

2/15
------
spawned 1 shoebox of aa+ and put one wbs pan cyan master to some woodchips for shits and giggles. I plan on using the other cyan jars I have for a straw/poo based sub. tested the aa+ and pan lc I had made a while back a couple days ago, both trash :shrug: Nocd two new lc's, hopefully have some better luck this time. still waiting on a bunch of other aa+ and pan cyan jars to finish colonizing.

2/26
-----
in anticipation of spawning some aa+ and cyan jars soon I decided to give a go at prepping and pasteurizing poo/straw subs.. have a few other qts from other projects that are ready to be spawned so lets see how it goes.

probably used a little to much straw in this mix but oh well:shrug:

3/14
---
been a while since ive updated as there wasnt much to say. no one constantly wants to hear the old " waiting on jars to finish colonizing" updates again and again. None the less spawned 8 tubs of aa+ today and two pan trays. I dont expect all 8 to fruit as spawn was a little bacterial. also found a new atl7 print so I started a few germ plates as I had originally planned on growing them for this. Will have a few more jars to spawn in a couple more days.


4/2
---
been way to busy working with all this rona shit going on to make any updates. torn between being lucky im still working and wanting to not have to work like everyone else :shrug:
none the less two aa+ tubs are close to done with first flush. tub one is full of some cool mutants, tub 2 is looking a lot better; im not expecting to get multiple flushes as these were a bit bacterial.
tub 1

tub 2

going to take clones and run them again; on transfer 2 or 3 on the atl7 cultures, ill have those to grain soon.



Edited by Nikoyo (04/02/20 02:37 PM)


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Re: Let's All Grow Mushrooms 2020 [Re: Nikoyo] * 1
    #26395526 - 12/22/19 12:14 PM (4 years, 1 month ago)

:regularshroom: Never-Eat-a-Amanita's LAGM 2020 Grow Log :regularshroom:


The Plan: Swipe spores from a psilocybe cubensis (vars. Koh Samui and Mars) print to agar, make transfers until a clean culture is acquired, go agar-to-grain with wheat jars, build shoeboxes, grow mushrooms.

January 1, 2020 (Day 1): Making little progress so far, but the beer is cold.


January 12: Was home from vacation for less than 24 hours before getting spores to agar. In an interesting twist, my unheated garage (where I store my myco gear) froze hard while I was away. This includes  the half-dozen spore syringes and half-dozen spore prints I had on hand. A quick search of the Shroomery archives turned up conflicting speculation on whether cubensis prints would be destroyed by freezing. Most people seem to think that spores suspended in water would be destroyed by freezing, but prints should be okay. I'm going to try to use the Koh Samui and Mars prints, as originally planned, but I'm also going to try a Transkei spore syringe (after thawing) just out of curiosity. I'm going to be pretty bummed if all of these spores are ruined.

Used some agar plates that I poured about a month ago using a recipe of 400 mL of water, 6 grams of LME, and 8 grams of telephone brand agar-agar. If I was going to make new plates for germination, I would probably have reversed that LME/agar-agar ratio, as the last time (first and only) time I tried scraping spores to plates they took a week to germinate. But these were what I had on hand.

Set up my SAB with a wire rack on top of four half-pint jars for height. Sprayed and wiped all tools with 70% iso. Sprayed the inside walls of the SAB lightly with soapy water. Took a quick shower and gargled with mouth wash.



Used a disposable inoculation loop to scrape up spores from prints, then spread on agar plates in a "z" pattern (from what I've been reading of this thread, swabs are more popular--I'll try that someday but have no sterilized swabs on hand at this point). Initially inoculated three plates each with Koh Samui and Mars spores. Switched loops between each print. Flamed loop on gas range between each plate, cooling in receiving plate. Inoculated three more plates with a small drop each from my Transkei syringe (shaking the syringe before starting and flaming needle tip between each pate).

At this point I realized that I had been spraying my hands and arms with SOAPY WATER AND NOT ALCOHOL. :rolleyes: So, after dousing myself with alcohol for real this time, I went back and used a new loop to scrape spores from the Koh Samui print to the two plates I had left.

Between the frozen spores and the poor sterile technique, I've definitely worried.

January 16: Four days after swiping spores to agar, I have what appears to be germination in one of my Mars plates and one of my Koh Samui plates. The Koh Samui germination is too faint to pick up with my cell phone camera, but you can just make out a spot of the Mars germination here:


No action in the Transkei MSS plates. Also, no indication of any contamination yet in any of the 11 plates--a relief after my SNAFU trying to decon with soapy water during inoculation.

January 18: Solid germination in all three Mars plates, but only one of five KS plates. Nothing in the Transkei plates. Poured new agar plates for upcoming transfers, using a recipe of 6 g LME, 8 g agar-agar, 400 mL water. Put dry ingredients in a 500-mL media jar, poured in boiling water incrementally, swirling bottle between pours to dissolve solids. Poured into 100-mm PP5 petris dishes, that will theoretically be reusable.These dishes didn't come with a sleeve and don't stack well so I went ahead and wrapped the dishes to keep them secure. Real bad condensation at the moment.

January 20: Made my first pre-pour agar plates using holy grail containers. I described my procedures here in Mr. Alien's thread, but long story short I ended up with six holy grail containers with minimal condensation, so decided to do my T1 transfers into these instead of the PP5 petri dishes I poured on the 18th.


January 21: Made my T1 transfers. Took three wedges from my best Koh Samui plate (a couple others have small points of germination but this one is by far the best).


And took two wedges from one of the Mars plates and one wedge from another Mars Plate.


Transferred the wedges to the holy grail pre-pour dishes I made on the 20th.


Fumbled a bit more than usual with the transfers today. Partly this was due to the holy grail containers being deeper than the petri dishes I'm used to, making it harder to slide the wedge off the scalpel. Also, the wire rack I'm using is pretty slippery, making it difficult to keep the donor dish from sliding around when I'm trying to make a cut. I would really like to find a better raised rack to use in my SAB.

January 25: Poured some new plates using standard, disposable, 100-mm, polystyrene petri dishes. I am about ready to make my T2 transfers, but am then going to be out of town for a week and a half or so. I am worried that if I transfer to HG containers, they might be overgrown by the time I can give them some attention. Used a recipe of 400 mL of water, 6 grams of LME, and 8 grams of telephone brand agar-agar.

January 26: Mars T1 plates are looking like this:


And Koh Samui T1 plates are looking like this:


Planning on making transfers tomorrow. Planning on targeting the rhizomorphic growth on the first Mars plate between 6:00 and 9:00. For the Koh Samui plate I'll be targeting the far right plate between 6:00 and 10:00. I believe that the small satellite at 7:00 is mycellium from where the wedge bounced around a bit, but I'll be avoiding it anyway. I'm cautiously optimistic that the far left Koh Samui T1 plate is clean enough for grain--it's not very rhizomorphic but it is very uniform in shape.

January 27: Made my second transfers. Took three wedges from one Mars plate:


And three wedges from one Koh Samui T1 plate:


Transferred to the 100-mm agar dishes I poured on the 25th.


Fingers crossed these will be looking nice and clean when I get home!

February 9: Been a while since I've had the chance to do any myco work, checking in on the transfers I made 13 days ago.


Decided to throw that very symmetrical Mars plate into the fridge before it outgrows the plate, and possibly send it straight to grain at a later date. When ahead and transferred three wedges from another Mars plate into Holy Grail containers.


Also transferred three wedges from Koh Samui plates. Had a harder time finding targets in these plates. Eventually decided to transfer one wedge from the most symmetrical of the plates (even though it's a bit patchy) and two from a particularly rhizomorphic sector in another plate.


February 16:One week after transferring, Mars T3s look pretty good. Plan on spawning the far right plate just as soon as I prep some grain jars. Hopefully on Tuesday.


Koh Samui plates still need some work. The far left plate has a wacky grow pattern because I accidentally butterflied the agar wedge open when it went to the plate. It does have some vigurous growth, though, so I think I'll take T4 transfers from it during my next SAB session.


February 17: Prepped some grain following Nate Dawg's tek. Rinsed two full quarts of hard red winter wheat, then placed the wheat in a large stock pot with enough hot water to allow the grain to double in size. Put on a hot burner and quickly brought to a rolling boil. Started checking grain hydration after ten minutes or so, ended up boiling for 16 minutes before draining with a square of window screen material set in the sink. Pulled the screen material out of the sink after draining for a few seconds, and spread out on the counter top on a towel. Tossed a few times and allowed to steam dry until the grain was cool and passed the toilet paper test.


Transferred the grain into five quart jars and one pint jar (all approximately 2/3 full). Capped the jars with plastic wide-mouth lids that have been modified with a 1/4-inch hole covered with a small circle of SFD, sealed with blue RTV silicone.


Placed the five jars into the PC, added three quarts of water. Vented steam for ten minutes, then brought up to 15 PSI and PC'd for two full hours. Allowed PC to depressurize on its own.

February 18: Took my best Mars plate to grain today. First I transferred a small wedge to a HG container so I can keep this culture on agar. Then I tic-tack-toed the remaining agar, and transferred several squares to each of four wheat jars (PC'd yesterday). I tried rolling and gently shaking the jars a bit to bury the agar and ended up with one square STUCK to the glass up above the grain. Nothing I could do to get the bastard off there :rolleyes:


Also made transfers from my butterfly Koh Samui T3 plate to three new HG containers. Hoping these finally clean up on T4.


February 28: Koh Samui T4 plates still not where I want them so I went ahead and made another round of transfers. Took one wedge out of each of my T4 plates and transferred to T5 plates.


Also, checking in on my Mars jars. All have some growth but are coming along pretty slowly--these pics are 11 days after inoculation.


March 9: Ten days since my last post and my Mars jars are coming along slooowwwly. Maybe they're getting close to ready for a shake? My best guess is that my wheat was under-hydrated. I pulled some grains out of a jar that I prepped at the same time but didn't inoculate, and many of them had bright white, uncooked grain in the center. Seems like I was erring on the side of pulling the grains early.


My Koh Samui T5 plates are interesting. I think that I'll take the far left plate to grain, since it's the only one that doesn't have areas that worry me, though it definitely is not symmetrical. But I will also probably do some transfers from the far right plate that is so rhizomorphic (with a troubling blank patch at 12:00).


Tonight I started soaking a couple of quarts of wheat in hot water with a tablespoon of gypsum. I will soak them for 24 hours then do a quick boil tomorrow, following Mr. Alien's Wheat Prep Tek. I'm sure that it was operator error the last time I tried to prep wheat following the no-soak method, but I'm still inclined to try something new. I plan on prepping six jars, and inoculating three with Koh Samui and three with a Mars plate I have been holding in the fridge. Idea being that the Mars jars may or may not ever finish up.

March 10: In the evening I rinsed the wheat I had soaking, then put it in a big pot with some hot water and brought to a simmer. After ten minutes of simmering, started checking some grains. Most were cooked, a few still had white cores, so I kept the simmer going for a couple additional minutes. Finally decided that the vast majority of the grains were fully cooked, so I drained in window screen material, then laid it all out on a counter on top of a towel. Let it steam dry, tossing occasionally, until the grains were dry on the outside.

Filled six quart jars two-thirds full, attached lids (with SFDs) and covered with foil. Placed in pressure cooker, vented for ten minutes, then PC'd at 15 PSI for two full hours. Left to cool on the stove overnight.

March 11: Pulled the two plates below (one KS and one Mars) out of cold storage. Used each plate to inoculate three of the wheat jars that I PC'd yesterday. I am hopeful that the wheat is more completely hydrated and these jars will colonize faster than the last batch.


March 12: Went ahead and shook up two of the Mars jars that were originally inoculated back on February 18 (23 days ago). These two jars were pretty clearly at least 25% colonized. The other two that were inoculated on February 18 are clearly further behind, on the order of 10-20% colonized. Pic is before and after shake.


March 14: Jars that I inoculated on the 11th are taking a while to get going but I am hopeful that is because I took the plates straight out of the fridge and didn't give them time to wake up before putting to grain. But today, I finally see the mycelium jumping off (both Mars and Koh Samui jars). 


Also, I'm reasonably happy with the recovery of the jars that I shook two days ago, look like they could really come together in a few days (fingers crossed!)


March 16: Shook a third Mars jar from the four that were inoculated back in February.

March 19: The jars that I shook a week ago have recovered and colonized almost 100%, I'm encouraged.


The jar that I shook three days ago is also recovering.


The fourth jar from that original batch of Mars jars inoculated on February 18 (a month ago!) has essentially stalled out, short of 25% colonization. Probably going to consider that guy a lost cause.


The second batch of inoculated jars are colonizing at various rates of speed...none close to 25% yet. Still haven't really got grain prep figured out, I guess.

March 26: I have three Mars jars now that are at like 99.9% colonization, but I still see partially uncolonized grain at the top of the jar. I dunno, maybe they're bacterial or maybe there's a hydration problem. But I recently had some really poor shoeboxes when I got impatient and spawned some jars that were not quite 100% where they needed to be, so I'm not going to mess with that anymore. These will sit until they get fully colonized or tossed.


Also decided to shake up my three Koh Samui jars, they had been spawned on the 11th and were pretty solidly at 25-30% colonization.

March 29: The three Koh Samui jars that I shook three days ago are recovering really nicely.


Also decided to go ahead and shake up the remaining four Mars jars that I had sitting around. The far right jar had been inoculated back on February 18 and is probably a lost cause. The other three jars were inoculated on March 11 and I'm a bit more hopeful about them.


April 11: Spawned two Mars shoeboxes today, using the SFF Shoebox Tek. Hydrated 272 grams of coco coir with 1.36 liters of boiling water in a preheated cooler in the morning, per Bod's Bucket Tek. In the evening I took the two Mars jars I have that are fully colonized (inoculated on March 11). Mixed each jar up in a 7-quart shoebox with coir at approximately a 1:1 ratio. Smoothed the surface, then added a pint of coir for a pseudocasing layer. Sprayed that layer per the tek. Both jars smelled nice and mushroomy, so I'm feeling okay about them despite how long they took to colonize. I've got three Koh Samui jars that are almost ready to go as well. Shit I might make it.



April 13: Spawned three more shoeboxes with three mycoquarts of Koh Samui spawn that were inoculated on 3/11. This time I measured how much water I sprayed on top of the top coat, something I've always been meaning to do. Shaper's SFF Shoebox Tek just directs you to spray for about 20 seconds with a fine mist sprayer, which is fine but I've been curious how much water that was. For me, this time with my sprayer, it was about 35 mL of water for each of the three boxes.


April 15: Mycelium is colonizing the two Mars boxes nicely, just starting to break through the top layer this morning.

April 16: Koh Samui mycelium is now breaking through the top layer of those boxes, and the Mars mycelium continues to spread. Trying to decide when I will first pop the lid and take a close look.

April 17: Opened up my Mars boxes for a look-see because the droplets were starting to dry up on the sides. Glad I did because both were looking a bit dry. Photos taken after misting, these are six days after spawning.


April 19: Similar six day check-in on my Koh Samui boxes; photos taken after a light misting.


April 22: Some pins showing up in both of my Mars boxes (left two pics). Doesn't look like it's going to be a super impressive pinset. Lots of hyphal knots in the Koh Samui boxes (right three pics) but also looks like they're making metabolites.


April 27: Looks like I'm going to get half-way decent flushes out of my Mars boxes. Veils started to break this afternoon, I think that I'll wait until morning to harvest just to see how far the caps open and maybe take some prints. Also plan on taking a clone from one of the larger clusters--these genetics seem to make clusters more than other strains I've managed to fruit.


The Koh Samui boxes are strange and disappointing. The fruits are showing up almost entirely as side and bottom pins. The strange things is that I would have thought I built and hydrated and tamped down these boxes almost identically to how I did the Mars boxes, which have like zero side pins. It makes me think that genetics plays a significant role in tendency to fruit on the sides vs. top surface. The other really weird thing that's going on with these KS boxes is there are strange bumps growing all over the surface. I posted a pic to the contamination forum a few days ago when I first noticed them and somebody said it looked like the mycelium was producing a ton of metabolites. But now that the bumps have grown they look less like metabolites. I wonder if these are what people refer to as "blobbing"?


April 28: Harvested both Mars shoeboxes today, taking about 196 wet grams out of one, and 203 wet grams out of the other. Selected three of the larger caps and started printing (my first time). I made three little 4x8-inch foil rectangles, folded in half. Wrapped them up in a foil sleeve and baked at 350 degrees Fahrenheit for 30 minutes, then put directly into a Tupperware container that had been cleaned with iso alcohol. In my SAB, I placed the caps on the foil squares inside the Tupperware--I'll leave them sealed up for 24 hours to print. I also selected one stem from a larger cluster, placed it into a Ziploc bag and set aside for cloning. I dunked each of the substrates for 4.5 hours. Accidentally dropped one of the substrates while draining it so I'm down to one Mars shoebox for a second flush.


In the evening I took the stem out of the fridge and into my SAB along with some agar plates. I split the stem in half lengthwise with my fingers, then used a sterilized scalpel to transfer bits of inner tissue to three plates.


April 29: After allowing printing for 24 hours, I took the Tupperware containers and a shoebox (freshly cleaned with iso) into my SAB. I removed the caps from foil, folded half of the foil loosely over the top of each print, and placed them in the shoebox. I put the lid on the shoebox and put it aside to let dry for a few days.

April 30: After picking a few early fruits on the 29th, I fully harvested all three of my Koh Samui shoeboxes today. It ended up being a pretty big effort to pull all of the sidepins and bottom pins without tearing up the substrate--but I ended up with between 181 and 193 wet grams from each of the three shoeboxes. Not terrible. I submerged each of the substrates for four hours and affixed lids to set them back for a second flush.


May 4: Brought the shoebox with the sporeprints into my SAB and folded up the foil squares. My first sporeprints ever, two look nice and dark but the third is faint.


May 5: I was doing transfers today for some other projects so I went ahead and made T1 transfers from my Mars clone plates today, even though the clone plates hadn't grown out too far. I transferred two wedges from the far left plate and one from the middle plate, all to new plates.


May 7: Harvested the second flush of my remaining Mars shoebox today, 9 days after dunking the substrate. Tried the "float tek" for harvesting for the first time, worked great. Helped me cut my stumps closer to the substrate surface. Got a total of 149 wet grams, and dunked the substrate for four hours and hoping for a third flush.


Also had an early bird in one of my Koh Samui shoeboxes. Nothing like the 50 or 100-gram monsters seen regularly on here, but definitely the biggest mushroom I've ever grown, weighing in at 24 grams.


Edited by nevereataamanita (05/07/20 05:10 PM)


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Invisibleeatyualive
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Re: Let's All Grow Mushrooms 2020 [Re: nevereataamanita] * 2
    #26395576 - 12/22/19 12:45 PM (4 years, 1 month ago)

:chief: Stropharia Cubensis: Eat’s Grow 2020β„’ :chief:

Stropharia cubensis print grown from this print taken in 2015.
It has also been given to a few around the boards that had good success. I figured I would pay her a visit once again to attempt to take prints and share this beauty with you all. The prints were revived from an old grow in 2003. It was then printed in 2015.

Backround
Stropharia revival




Brought to you by the TRIBE


β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
January 01, 2020
I swabbed a 2015 OG SPORE PRINTβ„’ to a pasty plate as shown below.
EAT SWABS PLATESβ„’



I plan on doing 1 transfer and then going directly liquid inoculation to grain if the culture looks good

β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
January 03, 2020

Spores show signs of germination.
β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
January 05, 2020

I made a transfer from the spore plate to a fresh plate.


β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
January 8, 2020

The night before I anticipate the plate almost being complete, I start making grain jars of oats. I made 14 quarts of oats for this inoculation. I only had this many empty to work with.

EAT’S OAT PREPβ„’
β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
January 9, 2020

Since this is a special event I’m trying a special new lid to pour my liquid inoculant. I found this on amazon. It’s an stainless steel pour spout for oil and vinegar that fits regular mouth jars.

First I hit the dabs really hard, put on the slackers, then began to work.

After 4 days the culture looked good and destroyed the plate. Sorry for the shitty pictures. Anyway the stropharia colonize so fast I don’t even have time to transfer before they climb the walls. So now I’m going to liquid inoculate. I generally try to use a plate that hasn’t touched the edges but this is what we have to work with for this grow log.

EAT LIQUID INOCULATESβ„’



I use the entire plate tiger dropped into a blender and pour lid assembly for inoculation. I then liquid inoculated 14 quarts of oat grain jars with the plate. I generally use 1 plate to 10 quarts. In this instance I could expand one plate to 30 quarts comfortably. But right now we are clone searching for good candidates. I normally don’t shake after liquid inoculation. I allow the liquid inoculate to colonize the quarts completely. This takes 7-10 days if I use one plate to 10 quarts without a shake. Since I’m expanding a little more, I’ll shake the jars after day 4 growth to speed things up since I’m using less inoculate per quart jar. Generally there is about 1 tablespoon poured per jar. This time it may vary with this new pour lid.

The lids are being wrapped in heavy duty aluminum foil. Make sure to be wary of the bottom air spout. This can poke a hole through the foil. I am pressure cooking the lid for 30 minutes at 15psi. And now it begins. Now it’s time for the elves.



We tested the pour lid two ways with 1/4 pint of water in a regular mouth half pint and one colonized pasty plate tiger dropped into the blender jar.

First one is the normal setting where the spout comes down about 1/4 inch below the bottom of the lid. This is how the lid is intended for use with oil. I’m assuming you want a delay with oil so a large amount doesnt come gushing all over the place. I also can’t get the remainder of the liquid out at the bottom of the batch and I feel it’s wasteful.



The 2nd way we put the spout flush with the bottom of the lid. This seems to pour a little better and more controlled. I’m not sure I like the cap it’s a little funky. But it does provide a nice cover between pours if you need to take a break and it’s automatic. You could use your gloved finger to push it closed as well. I want a quick  motion where I flick once and it lets out as much inoculant as I need per jar. So what I’ve done in the image below is sit the spout flush with the bottom of the lid.



Ok test one with the bottom of the pour spout flush with lid is complete.  I liquid inoculated stropharia to oat spawn.

Pour lid and blender base swap


I do a slight swirl right before the first pour. If you keep the speed consistent after, the movement of the pour jar moves the mycelia slurry around for you. If you need to pause between jars for whatever reason. Simply do a light swirl again. I’ll work on getting a video of it. Things were foggy and hard to see with what I made.



Swirl technique

Inoculation


So the pour was nice and controlled. It poured less water than I’m used to but I absolutely love the automatic closing lid between each pour. It’s also really convenient if you lift the lid of receiving jar at a 45degree angle turn the pour lid and pour. When you lift the pour jar up it automatically closes and as that happens you are closing the lid to receiving grain jar. I slightly tighten each lid after the pour with my left hand while I’m doing all pour work with my right.

What I didn’t like is that 90% of the jar pours but the last 10% won’t pour. Even if you flip the entire jar 180 to the surface of the work area. You would have to free pour and that is not the idea. I’ll likely just increase my volume of water 1/4 inch more in the jar next time to account for the wasted liquid.


I also shook each jar at least a minute while spinning. Shake, spin, shake spin or roll as you shake.


I also inoculated jars using my old blender pour lid. I anticipate it will colonize faster.

β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”

January 18, 2020

The jars i did with my old pour lid are colonized in 8 and being spawned day 9. The jars with the new lid are colonizing 30% on day 8. So i am now shaking the jars.





Once the quarts are colonized, I prepped my substrate for spawning 3 tubs. I used 4 small bricks coir, 12 cups coarse grade vermiculite, 16.5 quarts water. I then spawned using 5 quarts spawn per tub. One tub only had 4 quarts spawn. I used a NO BOIL substrate prep.

EAT’S UNBUCK3T TEKβ„’

30 minutes after substrate prep I spawned.

EAT SPAWNSβ„’

For fruiting I am using Pasty’s ez micropore dialed tubs





Grow Log

:chief:Tub 1:chief:
4 quarts oat spawn to
Top layered at spawn
Micropore tape

      Day 5                  Day 11                Day 12              Day 13   



      Day 14                  Day 16                  Day 18              Day 14   

β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”
:chief:Tub 2:chief:
5 quarts oat spawn
Top layered at spawn
Micropore tape

      Day 5                  Day 11                Day 12              Day 13   



      Day 14                  Day 16                Day 18              Day 14   


β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”

:chief:Tub 3:chief:
5 quarts spawn
No top layer at spawn
Micropore tape

      Day 5                  Day 11                Day 12              Day 13         



      Day 14                Day 16                Day 18              Day 14         

β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”β€”

I’ve been harvesting with scissors like this.



Edited by eatyualive (01/23/20 07:50 PM)


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InvisibleInfiniteDreams
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Posts: 1,224
Re: Let's All Grow Mushrooms 2020 [Re: eatyualive] * 1
    #26395947 - 12/22/19 04:46 PM (4 years, 1 month ago)

Results: 55 days to harvest.

Day 1: 2/1 - spores to agar.  But actually 2/4 spores to agar for the one that finished first.

Variety: Ecuador

Agar plate ready to go to jar.  This variety was so easy to get healthy aggressive rhizomorphic mycelium!

2/24: 


Using oats, the myc loves this stuff.

3/8:



Jar birthed to shoebox on 3/10. 

20 days later and first flush ready to harvest:


3/30




Time for dinner:


Edited by InfiniteDreams (04/01/20 01:38 PM)


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InvisiblemushboyMDiscord
modboy
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
    #26395957 - 12/22/19 04:53 PM (4 years, 1 month ago)

Quote:

ComebackKid said:
-We start January 1st spores to agar





In case people forgot:shake:


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InvisibleCaps McGee
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Registered: 10/28/17
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Re: Let's All Grow Mushrooms 2020 [Re: mushboy]
    #26396070 - 12/22/19 06:00 PM (4 years, 1 month ago)

:whathesaid:

It didn't seem to say "earliest convenience" to me either lol


--------------------
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Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 5 days
Re: Let's All Grow Mushrooms 2020 [Re: Caps McGee]
    #26396090 - 12/22/19 06:12 PM (4 years, 1 month ago)

Quote:

ComebackKid said:
If you cant start for a couple weeks that's fine. Grab a spot and follow along in the meantime.
People are going to be "fumbling" at different stages of the grow.





CBK was being cool about it but yeah, I get why the concept loses meaning with a staggered start. I'll just watch.


--------------------
My LAGM 2020 Journal


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