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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
help with agar
    #26393852 - 12/21/19 11:05 AM (4 years, 1 month ago)

Not sure if any of this is salvageable mycellium. i have been away from agar for about 2 years now, i know there is a bunch of contamination and the pics are shitty but theyre the best i can do with my equipment :-)



thanks in advance!


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
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Re: help with agar [Re: numnum59]
    #26393928 - 12/21/19 12:03 PM (4 years, 1 month ago)

Hmmm... you're not wrong. There is some funk going on in those plates.

If you're transferring from those plates I'd use these to spots..



You also may have better luck starting over with new plates.  :shrug:  or both.

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
Re: help with agar [Re: BLINKfan420]
    #26393949 - 12/21/19 12:19 PM (4 years, 1 month ago)

thats what i was thinking too man. transfers complete lets see how this plays out thanks for your help. will keep this updated!


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
Re: help with agar [Re: numnum59]
    #26402875 - 12/27/19 11:03 AM (4 years, 1 month ago)



after one transfer. i think i may need to do 1 or two more transfers and itll be ready for a2g. (the bacteria looking shit on the right is on top of the lid not in the agar)

what exactly am i looking for in agar grow? any suggestion would be awesome! thank you!


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 10 days, 11 hours
Trusted Cultivator
Re: help with agar [Re: numnum59]
    #26402880 - 12/27/19 11:07 AM (4 years, 1 month ago)

there is still sporulating mold on that plate.

I would try for 1 of the 2 left most colonies and I would try and get a sample from the left most edge away from everything

work inside a SAB not a flow hood for this


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
Re: help with agar [Re: Camera93]
    #26402887 - 12/27/19 11:09 AM (4 years, 1 month ago)

Quote:

Camera93 said:
there is still sporulating mold on that plate.

I would try for 1 of the 2 left most colonies and I would try and get a sample from the left most edge away from everything

work inside a SAB not a flow hood for this




yeah, i see the mold growing. i was planning on doing a few more transfers. there is significantly less than on the other two plates for sure.
what should i look for as far as the mycellium itself on the plate?


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 10 days, 11 hours
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Re: help with agar [Re: numnum59]
    #26402891 - 12/27/19 11:13 AM (4 years, 1 month ago)

yeah man good job on that, I had my reply before I really read the OP
my apologies :hatsoff:

at this stage, I would just be focused on getting a clean plate without other growth

once you have that established, I look for fastest growing edges to transfer from
people say rhyzo growth is another characteristic to chase after if thats your thing.

but till then, I wouldn't limit myself on where to transfer from, I would just do it farthest away from any satellite colonies

AGAR ENVY! (Anything and All things agar!) - this isnt a tek on what it should or shouldnt look like, but there are tons of examples and talk on ideal transfer spots ect
pretty much as the title states, all things agar


Edited by Camera93 (12/27/19 11:15 AM)


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
Re: help with agar [Re: Camera93]
    #26402897 - 12/27/19 11:16 AM (4 years, 1 month ago)

will do thanks for your help :thumbup:

ill keep this updated!


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
Re: help with agar [Re: numnum59]
    #26426991 - 01/10/20 05:55 PM (4 years, 19 days ago)

told you guys id do an update, here it is



got two plates of beautiful myc on agar. did some A2G xfers already everything looking good. like riding a bike!! :thumbup:


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OfflineBLINKfan420
Jedi
I'm a teapot


Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 16 hours
Re: help with agar [Re: numnum59]
    #26427125 - 01/10/20 07:07 PM (4 years, 19 days ago)

:asianofapproval:

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
Re: help with agar [Re: BLINKfan420]
    #26432182 - 01/13/20 08:58 PM (4 years, 16 days ago)

the A2G transfers are growing, albeit slowly. i think my grains are a little on the dry side :/


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 21 days
Re: help with agar [Re: numnum59]
    #26465586 - 02/02/20 04:22 PM (3 years, 11 months ago)

Decided to restart with grains, they were def dry. The newer wetter grains are doing awesome. Well enough for a g2g to be successful


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