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Offlinemartinb
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Spores On Agar Visual Question
    #26392531 - 12/20/19 02:03 PM (4 years, 1 month ago)

Hi Guys,

I attempted my first spore swab to agar 5 days ago. I'm trying to work out if the bigger spot on the top left of the photo is healthy mycelium or a contam.

In the right lighting it kind of has a shine over it.

Was hoping to get your thoughts?



Thanks
Martin


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InvisibleFiatFirmamentum
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Re: Spores On Agar Visual Question [Re: martinb]
    #26392544 - 12/20/19 02:09 PM (4 years, 1 month ago)

Bacterial contamination, and you are unlikely to see growth from non-fresh spores after 5 days. I waited 2 weeks for my first mycelium to be visible.


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OfflineCybin_man
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Re: Spores On Agar Visual Question [Re: FiatFirmamentum]
    #26392568 - 12/20/19 02:33 PM (4 years, 1 month ago)

Let it grow out and you will hopefully be able to tell what’s mycelium and what’s not


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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc



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OfflineSFS96
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Re: Spores On Agar Visual Question [Re: Cybin_man]
    #26392664 - 12/20/19 03:43 PM (4 years, 1 month ago)

Still kinda hard to tell. I think the top left spot is bacteria but it does look like there’s some germination on the plate. Give it a couple of more days and you will be able to tell.


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How I make and preserve tea


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Offlinemartinb
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Re: Spores On Agar Visual Question [Re: SFS96]
    #26397850 - 12/23/19 04:14 PM (4 years, 1 month ago)

Whatever it was looks like it's been killed off?



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InvisibleInfiniteDreams
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Re: Spores On Agar Visual Question [Re: martinb]
    #26397867 - 12/23/19 04:23 PM (4 years, 1 month ago)

So did you use an inoculation loop?  Does that line pattern on the agar follow the streak pattern you used?

If so I'd grab something quick, I"m thinking the 5 to 6 oclock region, and do a transfer.


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Offlinemartinb
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Re: Spores On Agar Visual Question [Re: InfiniteDreams]
    #26397901 - 12/23/19 04:43 PM (4 years, 1 month ago)

It was a spore swab. But yeah it 100% follows the pattern I followed.

You reckon do a transfer now or let it grow out some more?


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InvisibleInfiniteDreams
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Re: Spores On Agar Visual Question [Re: martinb]
    #26397935 - 12/23/19 05:01 PM (4 years, 1 month ago)

Since spores are dirty from the get go I'd recommend grabbing some of the best growth straight away and transferring to a clean plate.  You want to isolate that clean mycelium.  If you go straight up from 6 oclock there looks to be a good rhizo cluster to grab.

I'm sure others have opinions to offer and take mine with a grain of salt.


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Invisiblewildernessjunkie
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Re: Spores On Agar Visual Question [Re: InfiniteDreams]
    #26398229 - 12/23/19 08:13 PM (4 years, 1 month ago)

I would make transfers now. For plates like this, its best to post some pics with light shining through the back of the plate too. It helps to identify contamination.

I would avoid those satellite colonies at 8 and 9 o'clock.


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Offlinemartinb
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Re: Spores On Agar Visual Question [Re: martinb] * 1
    #26508345 - 02/28/20 03:02 PM (3 years, 10 months ago)

Thought I'd share the final result of my first grow using these spores on agar. Pretty happy :smile:



Edited by martinb (02/28/20 03:05 PM)


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OfflineBsfixit
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Re: Spores On Agar Visual Question [Re: martinb]
    #26508440 - 02/28/20 03:30 PM (3 years, 10 months ago)

Nice job for first time !


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OfflineSockadin
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Re: Spores On Agar Visual Question [Re: martinb]
    #26508534 - 02/28/20 04:25 PM (3 years, 10 months ago)

Quote:

martinb said:
Whatever it was looks like it's been killed off?







More than likely it is not killed off and it is now hiding with your mycelium. Don't transfer from the area in question. I use a sharpie to mark bad spots so after you get growth you know where to transfer and where not to. I will X bad spots and O good spots.


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