Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Cloning to grain
    #26391141 - 12/19/19 05:35 PM (4 years, 1 month ago)

So it's simple, take an agar pin and drop it on grain.
Could easily go to another agar plate but the way I see it you'll get 5 times as much mycelium far faster.
So I dropped my pin

Rolled the jar to try get it to the middle
Day 2



Few days later


Shook it up...
Day 10




Day 12

So from pin to colonized in 12 days...to put that in perspective that's lime putting one grain in a jar for g2g but pin growth is super aggressive.
Here's the same culture( different plate) at about 70% on day 12 with half a dish for inoculant.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineCybin_man
Circle the Wagons


Registered: 05/02/17
Posts: 799
Loc: In the bathroom
Last seen: 3 years, 10 months
Re: Cloning to grain [Re: cronicr]
    #26391169 - 12/19/19 05:47 PM (4 years, 1 month ago)

Pretty swift. Have you ever done that before this?


--------------------
mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc



Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: Cloning to grain [Re: Cybin_man]
    #26391171 - 12/19/19 05:48 PM (4 years, 1 month ago)

Yeah often and I also drop them strait to lc


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineSamiam76
Stranger
 User Gallery


Registered: 08/29/19
Posts: 86
Last seen: 2 years, 9 months
Re: Cloning to grain [Re: cronicr]
    #26391175 - 12/19/19 05:51 PM (4 years, 1 month ago)

All I need  now is an agar pin.


Extras: Filter Print Post Top
InvisiblePsilosion


Registered: 03/23/17
Posts: 485
Re: Cloning to grain [Re: Samiam76]
    #26391180 - 12/19/19 05:53 PM (4 years, 1 month ago)

Good to know, I might try this when I get a pin.


Extras: Filter Print Post Top
Offlinenarmaduke
Stranger


Registered: 08/23/17
Posts: 197
Last seen: 1 year, 1 month
Re: Cloning to grain [Re: Psilosion]
    #26391206 - 12/19/19 06:07 PM (4 years, 1 month ago)

so this is a pin that grew off an agar dish?


Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Cloning to grain [Re: narmaduke]
    #26391268 - 12/19/19 06:29 PM (4 years, 1 month ago)

Quote:

narmaduke said:
so this is a pin that grew off an agar dish?



:whathesaid: ??

EDIT: We wonder because of how dirty a pin that's has been fruited in non-sterile conditions can be, straight to grains or LC. In other words you have some nuts cron.


Edited by Mr. Alien (12/19/19 07:28 PM)


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,385
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Cloning to grain [Re: Mr. Alien]
    #26391299 - 12/19/19 06:53 PM (4 years, 1 month ago)

Yes use agar pins.


Quote:

cronicr said:
Yeah often and I also drop them strait to lc




:awedance:


Edited by mushboy (12/19/19 06:54 PM)


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: Cloning to grain [Re: mushboy]
    #26391339 - 12/19/19 07:18 PM (4 years, 1 month ago)

Maybe I shouldn't have thrown all my old plates away that I was done transferring from..

So basically you have no idea what your going to get though right?

How do you know what that pin may or may not have grown to be?

Isn't that quite the gamble?


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: Cloning to grain [Re: SpunkyMonkey88]
    #26391340 - 12/19/19 07:19 PM (4 years, 1 month ago)

Unless it came off a clone plate


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
Trusted Cultivator
Re: Cloning to grain [Re: Mr. Alien]
    #26391347 - 12/19/19 07:21 PM (4 years, 1 month ago)

i have actually cloned straight to grain b4 by tissue sample i was all out of agar and had a fruit that i couldnt let get  away so against my better judgement i tried it and lo n behold 4 of 4 jars took and had a great grow from it


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
InvisibleMr. Alien
I will abduct andprobe your anus
 User Gallery


Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
Re: Cloning to grain [Re: mushboy]
    #26391372 - 12/19/19 07:31 PM (4 years, 1 month ago)

ohhh a fruit from an agar plate? for a second i thought he was using a pin fruited in dirty conditions :P I was like it can't be.


Extras: Filter Print Post Top
OfflineMadcapper144
Drummer
Male User Gallery

Registered: 01/05/18
Posts: 266
Last seen: 6 months, 27 days
Re: Cloning to grain [Re: jcm4620]
    #26391392 - 12/19/19 07:44 PM (4 years, 1 month ago)

Quote:

jcm4620 said:
i have actually cloned straight to grain b4 by tissue sample i was all out of agar and had a fruit that i couldnt let get  away so against my better judgement i tried it and lo n behold 4 of 4 jars took and had a great grow from it




I have some brf jars I cased with coir and am just trying to get some pins to clone. I have a bunch of agar dishes but I have 2 different lc's I was going to put a pin to as well. The lc is just for shits and giggles but do you think then this has a chance or am I crazy. Could have sworn Ivr read about people putting pins to lc but maybe that was just agar pins?


--------------------
Buy the ticket, take the ride!! :fearandloathing:


Extras: Filter Print Post Top
InvisibleSpunkyMonkey88
Stranger
Male


Registered: 10/08/19
Posts: 1,331
Re: Cloning to grain [Re: Mr. Alien]
    #26391393 - 12/19/19 07:44 PM (4 years, 1 month ago)

Quote:

Mr. Alien said:
ohhh a fruit from an agar plate? for a second i thought he was using a pin fruited in dirty conditions :P I was like it can't be.




No he was talking about invitro agar pins


Extras: Filter Print Post Top
InvisibleSmartattack
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,776
Loc: A thought
Trusted Cultivator
Re: Cloning to grain [Re: SpunkyMonkey88]
    #26391400 - 12/19/19 07:47 PM (4 years, 1 month ago)

No he was'nt but could also work if clean.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


Extras: Filter Print Post Top
OfflineSamiam76
Stranger
 User Gallery


Registered: 08/29/19
Posts: 86
Last seen: 2 years, 9 months
Re: Cloning to grain [Re: cronicr]
    #26391640 - 12/19/19 10:25 PM (4 years, 1 month ago)

how long did it take to get a pin on agar?


Extras: Filter Print Post Top
InvisibleNobler Hino
a dojo and a forge?!
 User Gallery

Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum Flag
Re: Cloning to grain [Re: Samiam76]
    #26391654 - 12/19/19 10:40 PM (4 years, 1 month ago)

That's cool! I've always wondered about this but never tried. I bet it feels naughty dropping a mushroom into a LC lol. Can't wait to try.


--------------------

"The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.”
– Maria Sabina


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: Cloning to grain [Re: SpunkyMonkey88]
    #26391662 - 12/19/19 10:48 PM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Maybe I shouldn't have thrown all my old plates away that I was done transferring from..

So basically you have no idea what your going to get though right?

How do you know what that pin may or may not have grown to be?

Isn't that quite the gamble?



We never know what our clones are gonna be they always need testing.
No I would never suggest using a pin in any other circumstance, invite pins from sterile conditions sure go for it but a pin from your fruiting chamber is gonna grow contans 9 times out of 10.
This particular culture I isolated from spores


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 2 years, 1 day
Trusted Cultivator
Re: Cloning to grain [Re: cronicr]
    #26391672 - 12/19/19 11:00 PM (4 years, 1 month ago)

Quote:

cronicr said:
Quote:

SpunkyMonkey88 said:
Maybe I shouldn't have thrown all my old plates away that I was done transferring from..

So basically you have no idea what your going to get though right?

How do you know what that pin may or may not have grown to be?

Isn't that quite the gamble?



We never know what our clones are gonna be they always need testing.
No I would never suggest using a pin in any other circumstance, invite pins from sterile conditions sure go for it but a pin from your fruiting chamber is gonna grow contans 9 times out of 10.
This particular culture I isolated from spores




exactly what cron said the only reason it even worked for me was cuz it was a tissue sample from the inside of a fruit. i do not advocate even doin that ever i only did it cuz i was in a pinch and the fact it worked was prolly mostly luck. always use agar if you can otherwise be prepared for failure cuz most likely thats what will happen if your taking a fruit from the fc.


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
InvisiblePsilosion


Registered: 03/23/17
Posts: 485
Re: Cloning to grain [Re: jcm4620]
    #26391681 - 12/19/19 11:17 PM (4 years, 1 month ago)

Yeah I've gambled before too cloning a pin growing on agar, but it went well and they were potent. Could have gone either way though.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* cloning ??? jba6511 613 4 04/18/03 05:19 AM
by Anno
* Whole Grains Knives_Out 559 3 11/04/02 03:38 PM
by bowling-name
* Difference between Rye flour and Rye grain Visigoth 1,745 1 03/25/02 04:31 PM
by ChromeCrow
* Getting poo to correct water content XiC_clone 1,245 15 10/31/02 04:18 AM
by DERRAYLD
* How long for grains? XiC_clone 1,206 9 10/10/02 09:12 PM
by XiC_clone
* mushrooms grown from spores or cloned with very low active ingredient content
( 1 2 all )
Spagiro 326 21 05/06/20 06:58 AM
by oculodextro
* Improving genetics by cloning clones gabbk 1,418 10 05/06/20 01:00 AM
by Baby_Hitler
* Question about cloning Visigoth 1,379 2 03/21/02 12:20 AM
by Zen Peddler

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
999 topic views. 37 members, 252 guests and 55 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.005 seconds on 14 queries.