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OfflinecronicrMFacebook
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Cloning to grain
    #26391141 - 12/19/19 05:35 PM (5 years, 29 days ago)

So it's simple, take an agar pin and drop it on grain.
Could easily go to another agar plate but the way I see it you'll get 5 times as much mycelium far faster.
So I dropped my pin

Rolled the jar to try get it to the middle
Day 2



Few days later


Shook it up...
Day 10




Day 12

So from pin to colonized in 12 days...to put that in perspective that's lime putting one grain in a jar for g2g but pin growth is super aggressive.
Here's the same culture( different plate) at about 70% on day 12 with half a dish for inoculant.


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OfflineCybin_man
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Re: Cloning to grain [Re: cronicr]
    #26391169 - 12/19/19 05:47 PM (5 years, 29 days ago)

Pretty swift. Have you ever done that before this?


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OfflinecronicrMFacebook
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Re: Cloning to grain [Re: Cybin_man]
    #26391171 - 12/19/19 05:48 PM (5 years, 29 days ago)

Yeah often and I also drop them strait to lc


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OfflineSamiam76
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Re: Cloning to grain [Re: cronicr]
    #26391175 - 12/19/19 05:51 PM (5 years, 29 days ago)

All I need  now is an agar pin.

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InvisiblePsilosion


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Re: Cloning to grain [Re: Samiam76]
    #26391180 - 12/19/19 05:53 PM (5 years, 29 days ago)

Good to know, I might try this when I get a pin.

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Offlinenarmaduke
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Re: Cloning to grain [Re: Psilosion]
    #26391206 - 12/19/19 06:07 PM (5 years, 29 days ago)

so this is a pin that grew off an agar dish?

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InvisibleMr. Alien
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Re: Cloning to grain [Re: narmaduke]
    #26391268 - 12/19/19 06:29 PM (5 years, 29 days ago)

Quote:

narmaduke said:
so this is a pin that grew off an agar dish?



:whathesaid: ??

EDIT: We wonder because of how dirty a pin that's has been fruited in non-sterile conditions can be, straight to grains or LC. In other words you have some nuts cron.

Edited by Mr. Alien (12/19/19 07:28 PM)

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InvisiblemushboyMDiscord
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Re: Cloning to grain [Re: Mr. Alien]
    #26391299 - 12/19/19 06:53 PM (5 years, 29 days ago)

Yes use agar pins.


Quote:

cronicr said:
Yeah often and I also drop them strait to lc




:awedance:

Edited by mushboy (12/19/19 06:54 PM)

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InvisibleSpunkyMonkey88
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Re: Cloning to grain [Re: mushboy]
    #26391339 - 12/19/19 07:18 PM (5 years, 29 days ago)

Maybe I shouldn't have thrown all my old plates away that I was done transferring from..

So basically you have no idea what your going to get though right?

How do you know what that pin may or may not have grown to be?

Isn't that quite the gamble?

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InvisibleSpunkyMonkey88
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Re: Cloning to grain [Re: SpunkyMonkey88]
    #26391340 - 12/19/19 07:19 PM (5 years, 29 days ago)

Unless it came off a clone plate

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Offlinejcm4620
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Re: Cloning to grain [Re: Mr. Alien]
    #26391347 - 12/19/19 07:21 PM (5 years, 29 days ago)

i have actually cloned straight to grain b4 by tissue sample i was all out of agar and had a fruit that i couldnt let get  away so against my better judgement i tried it and lo n behold 4 of 4 jars took and had a great grow from it


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InvisibleMr. Alien
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Re: Cloning to grain [Re: mushboy]
    #26391372 - 12/19/19 07:31 PM (5 years, 29 days ago)

ohhh a fruit from an agar plate? for a second i thought he was using a pin fruited in dirty conditions :P I was like it can't be.

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OfflineMadcapper144
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Re: Cloning to grain [Re: jcm4620]
    #26391392 - 12/19/19 07:44 PM (5 years, 29 days ago)

Quote:

jcm4620 said:
i have actually cloned straight to grain b4 by tissue sample i was all out of agar and had a fruit that i couldnt let get  away so against my better judgement i tried it and lo n behold 4 of 4 jars took and had a great grow from it




I have some brf jars I cased with coir and am just trying to get some pins to clone. I have a bunch of agar dishes but I have 2 different lc's I was going to put a pin to as well. The lc is just for shits and giggles but do you think then this has a chance or am I crazy. Could have sworn Ivr read about people putting pins to lc but maybe that was just agar pins?


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InvisibleSpunkyMonkey88
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Re: Cloning to grain [Re: Mr. Alien]
    #26391393 - 12/19/19 07:44 PM (5 years, 29 days ago)

Quote:

Mr. Alien said:
ohhh a fruit from an agar plate? for a second i thought he was using a pin fruited in dirty conditions :P I was like it can't be.




No he was talking about invitro agar pins

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InvisibleSmartattack
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Re: Cloning to grain [Re: SpunkyMonkey88]
    #26391400 - 12/19/19 07:47 PM (5 years, 29 days ago)

No he was'nt but could also work if clean.


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OfflineSamiam76
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Re: Cloning to grain [Re: cronicr]
    #26391640 - 12/19/19 10:25 PM (5 years, 29 days ago)

how long did it take to get a pin on agar?

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InvisibleNobler Hino
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Re: Cloning to grain [Re: Samiam76]
    #26391654 - 12/19/19 10:40 PM (5 years, 29 days ago)

That's cool! I've always wondered about this but never tried. I bet it feels naughty dropping a mushroom into a LC lol. Can't wait to try.


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OfflinecronicrMFacebook
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Re: Cloning to grain [Re: SpunkyMonkey88]
    #26391662 - 12/19/19 10:48 PM (5 years, 29 days ago)

Quote:

SpunkyMonkey88 said:
Maybe I shouldn't have thrown all my old plates away that I was done transferring from..

So basically you have no idea what your going to get though right?

How do you know what that pin may or may not have grown to be?

Isn't that quite the gamble?



We never know what our clones are gonna be they always need testing.
No I would never suggest using a pin in any other circumstance, invite pins from sterile conditions sure go for it but a pin from your fruiting chamber is gonna grow contans 9 times out of 10.
This particular culture I isolated from spores


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinejcm4620
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Re: Cloning to grain [Re: cronicr]
    #26391672 - 12/19/19 11:00 PM (5 years, 29 days ago)

Quote:

cronicr said:
Quote:

SpunkyMonkey88 said:
Maybe I shouldn't have thrown all my old plates away that I was done transferring from..

So basically you have no idea what your going to get though right?

How do you know what that pin may or may not have grown to be?

Isn't that quite the gamble?



We never know what our clones are gonna be they always need testing.
No I would never suggest using a pin in any other circumstance, invite pins from sterile conditions sure go for it but a pin from your fruiting chamber is gonna grow contans 9 times out of 10.
This particular culture I isolated from spores




exactly what cron said the only reason it even worked for me was cuz it was a tissue sample from the inside of a fruit. i do not advocate even doin that ever i only did it cuz i was in a pinch and the fact it worked was prolly mostly luck. always use agar if you can otherwise be prepared for failure cuz most likely thats what will happen if your taking a fruit from the fc.


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InvisiblePsilosion


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Re: Cloning to grain [Re: jcm4620]
    #26391681 - 12/19/19 11:17 PM (5 years, 29 days ago)

Yeah I've gambled before too cloning a pin growing on agar, but it went well and they were potent. Could have gone either way though.

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