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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 5 months, 26 days
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Weak growth in grain jars
#26390510 - 12/19/19 11:11 AM (4 years, 1 month ago) |
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Will old grains cause weak growth?
I inoculated some BRF jars (TC and B+), and they grew fine. I transferred some of the colonized BRF material to grain jars, but it seems slow and weak in its growth rate. The remainder material in the BRF jars pinned, so I don't think it's an issue regarding the spore source. My grains get an overnight soak, routine sterilization, and have filter-disc lids. Jars are kept in a room at 75% humidity, mild circulating air, 12-on/12-off lighting, and room temp is 75F +/-2F
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Mycoactive
Scientist


Registered: 11/20/19
Posts: 185
Last seen: 27 days, 58 minutes
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Re: Weak growth in grain jars [Re: CliftonGK1]
#26390528 - 12/19/19 11:24 AM (4 years, 1 month ago) |
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Quote:
CliftonGK1 said: Will old grains cause weak growth?
I inoculated some BRF jars (TC and B+), and they grew fine. I transferred some of the colonized BRF material to grain jars, but it seems slow and weak in its growth rate. The remainder material in the BRF jars pinned, so I don't think it's an issue regarding the spore source. My grains get an overnight soak, routine sterilization, and have filter-disc lids. Jars are kept in a room at 75% humidity, mild circulating air, 12-on/12-off lighting, and room temp is 75F +/-2F
They'd have to be REALLY old (years) to have substantial nutrient loss, so it shouldn't be an age-related thing. Sometimes a change in nutrients (e.g., going from BRF to grain) can slow growth for a bit as the myc has to start producing some different enzymes to digest the new food source. Your process sounds good to me. I'd just give it time and see if things speed up.
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 5 months, 26 days
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Re: Weak growth in grain jars [Re: Mycoactive]
#26390554 - 12/19/19 11:37 AM (4 years, 1 month ago) |
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Quote:
Mycoactive said:
They'd have to be REALLY old (years) to have substantial nutrient loss, so it shouldn't be an age-related thing. Sometimes a change in nutrients (e.g., going from BRF to grain) can slow growth for a bit as the myc has to start producing some different enzymes to digest the new food source. Your process sounds good to me. I'd just give it time and see if things speed up.
These were the tail end of a bag which was somewhere between 15 and 18 months old. So since you've mentioned *years*, it might be the case that these were a bit on the degraded side. They looked OK; no bugs, no mold/must, no funky smell. I've received a new bag and I'm starting a soak today. I'll see how one jar from each set does on a G2G split into the new batch.
Thanks!
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Mycoactive
Scientist


Registered: 11/20/19
Posts: 185
Last seen: 27 days, 58 minutes
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Re: Weak growth in grain jars [Re: CliftonGK1]
#26390557 - 12/19/19 11:39 AM (4 years, 1 month ago) |
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Quote:
CliftonGK1 said:
Quote:
Mycoactive said:
They'd have to be REALLY old (years) to have substantial nutrient loss, so it shouldn't be an age-related thing. Sometimes a change in nutrients (e.g., going from BRF to grain) can slow growth for a bit as the myc has to start producing some different enzymes to digest the new food source. Your process sounds good to me. I'd just give it time and see if things speed up.
These were the tail end of a bag which was somewhere between 15 and 18 months old. So since you've mentioned *years*, it might be the case that these were a bit on the degraded side. They looked OK; no bugs, no mold/must, no funky smell. I've received a new bag and I'm starting a soak today. I'll see how one jar from each set does on a G2G split into the new batch.
Thanks!
Sounds interesting. I'm curious to see the comparison. Please post when you have results!
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Weak growth in grain jars [Re: CliftonGK1]
#26390558 - 12/19/19 11:39 AM (4 years, 1 month ago) |
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99% chance it's your culture and not your grain. Standing by that number.😉
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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CliftonGK1
Sasquatch



Registered: 03/27/13
Posts: 327
Loc: A place
Last seen: 5 months, 26 days
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Re: Weak growth in grain jars [Re: Smartattack]
#26390583 - 12/19/19 11:48 AM (4 years, 1 month ago) |
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Quote:
Smartattack said: 99% chance it's your culture and not your grain. Standing by that number.😉
Always a possibility. I just thought it odd that it grew plenty strong on BRF, then just puttered along when transferred to grain.
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