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OfflineShr00merN00ber
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First time will attempt cloning
    #26387985 - 12/18/19 01:16 AM (4 years, 1 month ago)

Hey guys, I recently got some growkits that are growing. I just got them because its much faster than starting out with spores and the genetics from this company are usually excellent. This time around I want to take a few tissue samples and grow them out on agar. Here is their current state:

Kit 1







Kit 2



I have three questions:

1) I'm in a dilemma. I'm not sure which one to pick for cloning. In 1st pic of kit 1, the largest and first/fastest mushroom to grow is on the bottom left side. Its the biggest one of them all atm. Both in girth and in height. On the other hand, there is a nice healthy looking cluster on the right side. Would you choose a tissue sample from a cluster or go with the biggest mushroom which pinned first, even though its surrounding genetics are not as good as the cluster?

2) In pic 3 of kit 1, on the left side, you can see many small knots. Will these all grow out to form shrooms or will they not all make it?

3) The grow kit instructions say to water the sides of the bag every day when the first mushrooms appear. I haven't put one drop of water from the day I received it, its been 10 days now. I think it's fine as there is an excess of water to the point of drops sliding down the sides and pins have formed and are growing. Does the sub look too dry or something to any of you?

Thanks for any input!


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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26388038 - 12/18/19 03:06 AM (4 years, 1 month ago)

1.  That’s easy.. clone both/all the ones you want

2.  Hard to say.. no one is going to be able to give you a definitive answer... if the conditions are right, a good amount of them will.  But odds are, not all will... the myc is kind of hedging it’s bets

3.  Nope.. doesn’t appear to be too dry at all.. doesn’t even appear to be be pulling away from the sides due to shrinkage which is pretty common.  No bluing either, I’d say you’re golden.

Faht


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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26388045 - 12/18/19 03:18 AM (4 years, 1 month ago)

Quote:

Shr00merN00ber said:
Hey guys, I recently got some growkits that are growing. I just got them because its much faster than starting out with spores and the genetics from this company are usually excellent. This time around I want to take a few tissue samples and grow them out on agar. Here is their current state:

Kit 1







Kit 2



I have three questions:

1) I'm in a dilemma. I'm not sure which one to pick for cloning. In 1st pic of kit 1, the largest and first/fastest mushroom to grow is on the bottom left side. Its the biggest one of them all atm. Both in girth and in height. On the other hand, there is a nice healthy looking cluster on the right side. Would you choose a tissue sample from a cluster or go with the biggest mushroom which pinned first, even though its surrounding genetics are not as good as the cluster?

2) In pic 3 of kit 1, on the left side, you can see many small knots. Will these all grow out to form shrooms or will they not all make it?

3) The grow kit instructions say to water the sides of the bag every day when the first mushrooms appear. I haven't put one drop of water from the day I received it, its been 10 days now. I think it's fine as there is an excess of water to the point of drops sliding down the sides and pins have formed and are growing. Does the sub look too dry or something to any of you?

Thanks for any input!





Hey man! I did the same thing, going from a kit to bulk...


Take samples from some of the shrooms in the cluster, you want that clustering clone :smile:)


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: mushhiehunter]
    #26388083 - 12/18/19 04:15 AM (4 years, 1 month ago)

Thanks for the reply guys, can't wait to get started on the real grow!


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26389856 - 12/19/19 12:13 AM (4 years, 1 month ago)

Update:

Kit 1







Kit 2




Anyone know why the biggest mushroom's cap split like this? Now it makes me feel like I shouldn't clone that one. Things with kit 2 are looking kinda bad. Can any one provide some input? Thanks!


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390245 - 12/19/19 08:03 AM (4 years, 1 month ago)

Update:

Just got back from work and the 'X' shaped cap splitting is getting even more pronounced and I would really like some advice on what to do. Should I pick it now or leave it? The gills look like they have not developed spores, yet it is bruising blue at the same time. I can see a type of 'veil' going down the stem of the mushroom but in my nooby opinion, this is not the veil cap that broke. Could someone more experienced take a look and let me know what to do?

Also, related to my main aim which is to clone, maybe this big fat (mutant?) might not be the way to go. I'm uploading some pic so you can see, the surrounding mushrooms look strange too.



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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390258 - 12/19/19 08:20 AM (4 years, 1 month ago)

Usually splitting like that means the fruits either dry (which they don’t look dry) or the mushroom grew so fast that the fruit couldn’t keep up with itself and split... at least when caps split at the edge that’s why.  Your probably ok to clone it, I don’t think it’s infected or anything.

I’d clone any of them.

Maybe someone else has a different/better answer. 

Faht


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: fahtster]
    #26390261 - 12/19/19 08:25 AM (4 years, 1 month ago)

Cheers Faht for the answer. What about that veil going down the stem? I was reading up about that 'growth spurt' theory. I was also thinking pseudomonas but no clue. Would you harvest that one now or wait?


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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390275 - 12/19/19 08:37 AM (4 years, 1 month ago)

I’d take it now.. how deep is the substrate?

Veils do different things.. no need to worry there.

I don’t see any blotching.. maybe don’t clone the one with the white tipped cap.

But shit, if you have the means, I’d clone them all (one from each cluster if clustered) and grow them out and have fun with it.  Keep it fun and you’ll never have a failure, only fun learning experiences.

Faht


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: fahtster]
    #26390288 - 12/19/19 08:42 AM (4 years, 1 month ago)

So true Faht, if it ain't fun, it shouldn't be done!


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: fahtster]
    #26390302 - 12/19/19 08:47 AM (4 years, 1 month ago)

Quote:

fahtster said:
I’d take it now.. how deep is the substrate?

Veils do different things.. no need to worry there.

I don’t see any blotching.. maybe don’t clone the one with the white tipped cap.

But shit, if you have the means, I’d clone them all (one from each cluster if clustered) and grow them out and have fun with it.  Keep it fun and you’ll never have a failure, only fun learning experiences.

Faht




Does taking a clone from a fruit in a cluster really make that big of a difference when you grow it out?


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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26390316 - 12/19/19 08:59 AM (4 years, 1 month ago)

Not all sub strains perform the same... depends on what you’re looking I suppose... but that’s I say clone them all and see what happens.

Faht


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: fahtster]
    #26390329 - 12/19/19 09:11 AM (4 years, 1 month ago)

I made my first attempt on some Pastyplates yesterday, to be ready for cloning. I made 6 plates in total, 2 have excess water that has formed a little pool, I tilted one so that you can see the extent of it. Do I discard the ones with excess water? 10g agar 10g LME 500 ml water.



Edited by Shr00merN00ber (12/19/19 09:12 AM)


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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390343 - 12/19/19 09:21 AM (4 years, 1 month ago)

That ones for someone else with pasty plate experience

Faht


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Offlinesan pedro guy
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Re: First time will attempt cloning [Re: fahtster]
    #26390361 - 12/19/19 09:32 AM (4 years, 1 month ago)

sometimes i get a little bit of water on top too, you can just turn it upside down and dump it inside the sab before you use it, or not, i’ve left water in there and it was fine :shrug:


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390394 - 12/19/19 09:57 AM (4 years, 1 month ago)

Quote:

Shr00merN00ber said:
I made my first attempt on some Pastyplates yesterday, to be ready for cloning. I made 6 plates in total, 2 have excess water that has formed a little pool, I tilted one so that you can see the extent of it. Do I discard the ones with excess water? 10g agar 10g LME 500 ml water.







Ya dude just dump out the water on your SAB floor next time you use them, if you did it right the water is sterile anyway.

Also once you have a culture that's grown out a bit it usually "eats" up any left over surface water that you couldn't dump..

I get some pooling even in my petris that I pour especially if I pour them on the hot side... it's not a big deal just make sure they stand upright, dont want the water leaking out around the lid... dont know if that could actally happen but if it did it could be a wick for contamination.

That looks like some thick ass agar btw, I made that mistake my first time too. Itll work but it's a bitch to work with.


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: fahtster]
    #26390398 - 12/19/19 09:59 AM (4 years, 1 month ago)

Quote:

fahtster said:
Not all sub strains perform the same... depends on what you’re looking I suppose... but that’s I say clone them all and see what happens.

Faht




But can you still get a nice flush from cloning a fat "lone ranger"?

Or will it generate sparse fruits?


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26390412 - 12/19/19 10:10 AM (4 years, 1 month ago)

This point exactly was just on my mind.


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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390457 - 12/19/19 10:41 AM (4 years, 1 month ago)

Sure.. the difference probably isn’t crazy substantial if a variety is prone to clustering etc anyway and they can definitely make up the difference in size if there’s fewer.  But if you have a preference, do that... it’s all up to you.. it can make a big difference or it might not also

Faht


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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390474 - 12/19/19 10:51 AM (4 years, 1 month ago)

Quote:

Shr00merN00ber said:
This point exactly was just on my mind.



What point?


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