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OfflineFungiFart
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New Grower - 2w No Growth - Not sure what I'm doing Wrong...
    #26388399 - 12/18/19 09:31 AM (4 years, 1 month ago)

Hello,

This is my first post and my first attempt at growing.

It has been almost 2 weeks and I still haven't seen any mycelium growth. During initial inoculation, I was very careful to avoid contamination and injected the recommended 2cc into all-in-one grow bags that I've purchased on Amazon. There are 3 bags in total, two have the same spores and the other is a different strain.  I've purchased the spore syringes from a highly reviewed source, so I do not think the spores are the issue.  After a week of nothing, I re-inoculated the bags with another 2cc at a different location.

My Step Up
I'm placing the bags in a clear tote, with a microfiber blanket over the tote to keep the light out.  My house is usually cold, so I'm using a temperature-controlled heating pad (for seeding) and it is set to 83 degrees.  The heating pad is located at the bottom of the tote and there is a plastic divider separating the heating pad and bags to avoid direct contact. 

Here are the bags I'm using:
1lb Boom! Mixed Substrate All in one Mushroom Grow Bags


Do you foresee any issues with what I'm doing? Am I being impatient?

Thanks!


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OfflineCrackatoa
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: FungiFart]
    #26388445 - 12/18/19 10:06 AM (4 years, 1 month ago)

Spores to premade grain bags is a crap shoot. They need light, the heater isn't necessary if your place is warm enough to not need a jacket they're probably fine. I would give a little while longer. But to keep your mind off of those, I would suggest getting into agar, or going brf cakes. Brf cakes are now forgiving when using a spore syringe.


--------------------

Crack's Tek's and Shit



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OfflineA.k.aM
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: Crackatoa]
    #26388630 - 12/18/19 12:07 PM (4 years, 1 month ago)

Yeah starting more projects can’t hurt.

Brf cakes are supposed to be safest from syringes but I personally would do wbs jars.

Spores can take weeks to germinate but I’ve never gone more than like 5 days without seeing growth when using a syringe.


--------------------
LAGM2020


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OfflineFungiFart
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: Crackatoa]
    #26388631 - 12/18/19 12:08 PM (4 years, 1 month ago)

Thanks for the info!  I'll start giving them some light.  haha my place is usually around 63 degrees with the heat on, so yeah I bundle up bit.

I actually have some agar in my fridge.  Should I just place a few drops on it and let it grow or do I need to extract a sample for a fresh mushroom?  I bought them to test the spores/bacteria throughout my house (it was better than expected).

Thanks again!


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OfflineCrackatoa
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: FungiFart]
    #26388642 - 12/18/19 12:18 PM (4 years, 1 month ago)

Shake the shit out of it and a single drop in the center. Do a few plates though.


--------------------

Crack's Tek's and Shit



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OfflineFungiFart
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: Crackatoa]
    #26388816 - 12/18/19 02:02 PM (4 years, 1 month ago)

Should I wait for the agar to come up to room temp before shaking?


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OfflineA.k.aM
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: FungiFart]
    #26388839 - 12/18/19 02:14 PM (4 years, 1 month ago)

No shake the syringe. You want it mixed well so you don’t get a ton of spores at once.


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LAGM2020


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OfflineFungiFart
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: A.k.a]
    #26388956 - 12/18/19 03:14 PM (4 years, 1 month ago)

haha sorry I had a bit of a stoned moment there. Thanks again for the advice.


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OfflineA.k.aM
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Re: New Grower - 2w No Growth - Not sure what I'm doing Wrong... [Re: FungiFart]
    #26388961 - 12/18/19 03:15 PM (4 years, 1 month ago)

Lol I figured but thought I’d reply just in case.

I was half hoping you’d try to do it with one drop of agar in the center of each plate.


--------------------
LAGM2020


Edited by A.k.a (12/18/19 03:16 PM)


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