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Jooby
Stranger



Registered: 11/13/19
Posts: 174
Last seen: 2 years, 1 month
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
#26437104 - 01/16/20 04:18 PM (4 years, 13 days ago) |
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Quote:
footpath said:

I just wanna say, you're the f'n man. Also, your documentation here is next level shit.
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Re: Let's All Grow Mushrooms 2020 [Re: Jooby]
#26437129 - 01/16/20 04:41 PM (4 years, 13 days ago) |
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Hopefully it helps someone. Or at least has a pretty face.
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Nikoyo
FUN GUY



Registered: 01/29/17
Posts: 288
Last seen: 6 days, 3 hours
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
#26437190 - 01/16/20 05:25 PM (4 years, 13 days ago) |
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T2 transfers done
 the aa+ t1 plates were looking nice and clean so ill let them grow out a bit more and get some grain jars prepped for them this weekend if i have time. The pans on the other hand are still rather un uniform so ill be waiting to see how t2 plates go before I start any lc. Was able to get my hands on a cheap impulse sealer and a few spawn bags so ill be able to start prepping some spawn bags for them.
-------------------- Gettin back to work
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 3 days, 4 hours
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Re: Let's All Grow Mushrooms 2020 [Re: Nikoyo]
#26437387 - 01/16/20 07:26 PM (4 years, 13 days ago) |
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Still nothing on my PF Redspore or PE plates, but the second LGT plate is much cleaner than the moldfest of 1 (though the myc there seems to be going over the mold!). Reckon I should take a second transfer from the spore plate where there's the denser myc? You can see where I took the first transfer on the back shot:
LGT2:  LGT1, 1.1:  
Edited by Thufir_Hawat (01/16/20 07:26 PM)
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Re: Let's All Grow Mushrooms 2020 [Re: Thufir_Hawat]
#26437433 - 01/16/20 07:58 PM (4 years, 13 days ago) |
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& Yet & Yet... 1 • 16 • 20• T2 Day 2 •  
  24hr growth. Cool. You'd think the bubbles would confuse your identifying contaminants, but they are quite obviously nothing akin to a living, multiplying organism. I redact what I had said about my agar being too nutritious - my other cultures are now running amok. Maybe I am just transferring mold around. Maybe it's just this community of spores. Hm. • T1 Day 10 •Plate 3 The plate I decided I'll take to grain. It developed a little bald spot around 4 o'clock there. Otherwise, steady sailing. Am I concerned about the cleanliness of this plate? Reasonably. Will it keep me from taking it to grain? Fuck no.
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Jarhead3521
Grindin’



Registered: 09/26/19
Posts: 514
Loc: Amongst the pines
Last seen: 5 months, 22 days
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Re: Let's All Grow Mushrooms 2020 [Re: Thufir_Hawat]
#26437434 - 01/16/20 07:59 PM (4 years, 13 days ago) |
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1/16/20 update
Nothing like some good Irish whiskey and agar!

Made some transfers and a tissue sample from a PESH harvest.
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
#26437516 - 01/16/20 09:08 PM (4 years, 12 days ago) |
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the j stands for jesus.2020 new years grow along
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 21 days
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Finalizing our move... gonna run back to the old house early in the am and do all my mush chores... I've got 3 jars ready to spawn and I'm betting all my maz plates are ready to go to grain... no worries tho... the new grow room / lab will be done tmrw hopefully!
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nevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 6 days
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Re: Let's All Grow Mushrooms 2020 [Re: roarkell]
#26437579 - 01/16/20 09:59 PM (4 years, 12 days ago) |
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January 16: Four days after swiping spores to agar, I have what appears to be germination in one of my Mars plates and one of my Koh Samui plates. The Koh Samui germination is too faint to pick up with my cell phone camera, but you can just make out a spot of the Mars germination here:

No action in the Transkei MSS plates. Also, no indication of any contamination yet in any of the 11 plates--a relief after my SNAFU trying to decon with soapy water during inoculation.
-------------------- My LAGM 2020 Journal
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Jarhead3521
Grindin’



Registered: 09/26/19
Posts: 514
Loc: Amongst the pines
Last seen: 5 months, 22 days
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I’ve been putting up some shit pictures in my log, no real good looking plates yet, I’ll do better. I’m a slack ass.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
#26438049 - 01/17/20 08:41 AM (4 years, 12 days ago) |
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Feeling the same. Started hot but haven't had time to post my third transfer. Not that the pics are even worth stopping to look at.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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trubblesome
Stranger



Registered: 11/09/19
Posts: 406
Last seen: 10 months, 17 days
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
#26438067 - 01/17/20 08:52 AM (4 years, 12 days ago) |
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These jars are bad.

but especially the one on the left:

since they all came from the same LC there aint no way I'm spawning them to bulk. I might case the two on the right in the jar and put a baggy over the top just to see, but the one on the left gets the PC treatment. I'll give them the weekend and see how they look, but man they are dogshit, they are nasty.

but that's why I did 2 LCs. The ones from PESA LC 2112 are still lookin good in their shoeboxes.
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bendrider
Dirt Monkey


Registered: 08/08/18
Posts: 190
Last seen: 3 months, 11 days
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Re: Let's All Grow Mushrooms 2020 [Re: trubblesome]
#26438199 - 01/17/20 09:42 AM (4 years, 12 days ago) |
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I’m sure in it for 2020. I have PE6 in grain jars, PE and Mazatepec on plates cleaning up nicely.
Happy 2020 everyone!
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Jarhead3521
Grindin’



Registered: 09/26/19
Posts: 514
Loc: Amongst the pines
Last seen: 5 months, 22 days
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Re: Let's All Grow Mushrooms 2020 [Re: trubblesome]
#26438241 - 01/17/20 10:03 AM (4 years, 12 days ago) |
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I think my grains go in the jars more wet than I think they are. Gonna build a bigger softer than the colander I use now, get em spread out a little more. I tend to have good bit of condensation in my jars, but not always smell bacterial and have fruited ok. But then there’s been a couple that look like pipe dope covered grains that I just toss outside.....
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 13 days, 6 minutes
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
#26438279 - 01/17/20 10:26 AM (4 years, 12 days ago) |
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8 hours, 4,000 miles, 35,000 miles high, finally home!
I'm a little worried about the tiny potato bits in the agar? But here we go at long last. IMHO getting this late a start has only helped. But just in time as the topic is shifting from agar to transfers!
.. ..
Cap's SAGARI style agar - 4g agar, 7g potato, 50% Oat broth, 400ml. Green food color cause....shrooms!
GO NINERS!
Edited by Inthepit (01/17/20 11:48 AM)
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Durgin
Amateur Mad Scientist



Registered: 02/23/19
Posts: 446
Last seen: 16 days, 19 hours
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521] 2
#26438344 - 01/17/20 11:04 AM (4 years, 12 days ago) |
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January 17th:
Behold: a picture of me, holding a handful of straw soaked in liquified cow shit. What kind of terrible life choices did I make to get here?

Let me explain. So after making up some bags of manure/straw/grain for the pans I'm working on (see my last post), I was thinking they looked a little muddy, and after the PC cycle they definitely felt too dense to colonize well. I brought this up in the cultivating pans thread, and Asura (after chastising me for saying I followed his tek when I used black kow instead of hpoo - I should've probably said "adapted" instead) suggested I do a "microwave test" to check the water content. So I opened one of the bags and got out 100g of the substrate, and proceeded to (slowly! remember that straw is flammable) microwave out all the moisture. According to Asura, ideal hydration should be around 63%, meaning that the dry weight should end up at 37g.
But not matter how long I cooked it, I couldn't get it below 48g, so if anything it seemed that meant my sub was too dry (that is, it was only 52% water). WTF? I thought about this a while, stared at the glass like The Thinker, then drank some mushroom tea, drank some more because I was impatient, realized I had too much when my brain started to feel like scrambled eggs, then during the comedown stared some more, and after what seemed like hours but was probably 5 minutes of contemplation, I realized the problem: the mix seemed too muddy because of all the mud!
(I realize that this next bit may seem obvious to some of you, but as a city kid, this part doesn't come as naturally to me).
That is, the difference between black kow and plain old cow manure is that black kow is composted. That means it's broken down by microbes into simpler forms. And what's the end product of that process, if allowed to continue indefinitely? Regular old dirt. And when you mix dirt and water, you get mud. (In the case of black kow, you also get some small rocks, some sticks, etc., but nevermind that for now.) So the problem I was having with my mix being too muddy was because it was actually mud, and obviously mushrooms aren't going to grow on that. It doesn't have much nutrition left, and it won't hold water.
That gave me an idea. For growing, we want to keep the least broken-down part of the manure, but not the most digested stuff. There's no perfect way to do that, but I figured there's probably a good proxy - size. Microbes break down organic material, not build it up, so presumably the smallest particles are the least useful ones. So I decided to try an experiment, for the sake of science.
(Note here that this is going entirely off-script from any tek, so don't try this yourself unless you're willing to risk possible---dare I say likely---failure of your entire grow. You've been warned!)
So step 1, I opened up both bags and dumped them in a bucket, added some extra dry straw to offset the black kow that's about to get dumped, and soaked it all in hot water for an hour or so (stirring occasionally). Part of the idea here is that there is something unique about the manure that pans like, and so if that nutrition / flavor / whatever they respond to is water soluble, we'd retain some of it by using the manure soak water (my god that's a disgusting series of words that I can't believe I just used) to hydrate the straw. Also, the soaking / stirring would hopefully help separate out the different black kow material by density, with the mud falling to the bottom.
From there, I started grabbing handfuls of the solid mix and dumping them into my strainer:

After the strainer was mostly full, I'd give the whole thing a long rinse (mixing it up a couple times as I go) until the water draining off was mostly clear. When I got to the bottom of the bucket, I had to actually dump the contents through the strainer (a lot of the wheat grains had fallen to the bottom and mixed with the mud), so that took a lot more rinsing than the rest.
But all in all, the resulting mixture looked way less muddy and more airy than the initial version, which is exactly what I was hoping for:

(If you look closely you can see some of the sticks and stuff I mentioned before. I started trying to sift through it all, but that got to be a hassle, and then I realized that sticks and stones are present pretty much anywhere mushrooms grow in the wild so I didn't need to make a big deal about it.)
After allowing it to drain through the strainer, I then spread out the resulting mix (now mostly straw and grains, supplemented with verm and whatever solid parts of the black kow escaped the rinse) across several bowls and baking sheets to dry a bit more:

I ended up putting the sheets in the oven at 300F for an hour to get them a bit drier, then mixed it all up in a larger bucket and added another few handfuls of straw to absorb any remaining excess moisture (since it still seemed pretty damp) before loading up into bags once again. The final product looks much, much better this time:

I PC'd these for a bit over 2 hours at around 17 PSI, with an extended cool-down (turned the stove to low until it reached about 7 PSI, then turned it off) to avoid popping the bags. After they cooled, I took a look and mixed it up a bit, and the final substrate / grain mix is much lighter and airier than the last version, while still maintaining plenty of moisture to grow in. So I'm optimistic about the potential here, but we'll see how it plays out.
Meanwhile, in other news... all the other plates are growing cleanly as expected. The 4 B+ soft agar plates should be ready over the weekend, and each is going to get dropped to a pint of wheat (already prepped) when they're done. I'm not looking for ideal genetics from these plates - I mostly just care that they're clean, and beyond that variety is the goal, since I'm ultimately aiming to get clones from this grow. So whatever grows out the quickest and the largest will get cloned, and then from there I'll get more deliberate - my goal is basically just to use the agar as a cleaner version of a multispore inoculation.
As far as the PE and pan plates go, they're all growing as expected, though it looks like a couple plates are more aggressive than others. I'll plan to transfer those in a couple days and ditch the slow ones, then if those grow out clean I'll dump them to wheat (+ LC for the pans) and we'll be on our way.
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Hillionaire
Stranger

Registered: 09/01/19
Posts: 34
Last seen: 3 years, 2 months
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid]
#26438561 - 01/17/20 01:02 PM (4 years, 12 days ago) |
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I'm interested in joining the 2020 funguy party. I'm growing a few strains and a complete myco newbie but love it so far. I'm growing : Golden Teacher, B+, Z strain, Cambodian, Brazilian, and soon Transkei. I have some liquid culture popping off that will be shot into a 50/25/25 mix of amended soil that I had premade for me. Coco/ Ag grade vermiculite (course)/ Earthworm compost. The mix was a hopeful mix to hold more moisture while I was learning how to guage its water needs. The agar work is done under a horizontal laminar flow from like 1937 or something... its old. Probably from the 80's haha. So far, I got some syringes from Ralphster, they were all kinds of different, some got pink lipstick, and cinnamin contamination looking problems, in Rye berries, in unicorn bags. I went back to the drawing board with agar and things got a lot more straight forward. I was careful to put as little squirts as possible from the syringes and if i didn't use the whole thing on the Nest agar plates, using MEA mix, I threw them away so not to have complications beyond my experience level. Pics soon to follow.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 13 days, 6 minutes
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
#26438825 - 01/17/20 03:57 PM (4 years, 12 days ago) |
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Fuckin A! Footpath, your journal is a joy to read!
"The plate I decided to take to grain. It developed a little bald spot around 4 o'clock there. Otherwise, steady sailing. Am I concerned about the cleanliness of this plate? Reasonably. Will it keep me from taking it to grain? Fuck no."

And I wish the three(?), shroomites heading for surgery all the best. And please update us!
Edited by Inthepit (01/17/20 04:06 PM)
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DcevLearn
Team Tomentose


Registered: 07/07/18
Posts: 330
Last seen: 5 months, 2 hours
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
#26438939 - 01/17/20 05:17 PM (4 years, 12 days ago) |
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Quote:
DcevLearn said: 1/17/2020
- Finally back in the lab to checkout the initial spore plates
- Making the first transfers, still using the old agar plates from mid-November

Updated my log - DcevLearns To Grow Stones in 2020 - First transfers
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2020 [Re: Durgin]
#26439059 - 01/17/20 06:36 PM (4 years, 12 days ago) |
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I had a few good laughs while reading that bag prep there Durgin
I would have been so damn frustrated if that happened to me
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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