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Jooby
Stranger



Registered: 11/13/19
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Re: Let's All Grow Mushrooms 2020 [Re: eatyualive]
#26417115 - 01/05/20 07:18 AM (4 years, 24 days ago) |
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Correct. I'll fix spelling.
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Nikoyo
FUN GUY



Registered: 01/29/17
Posts: 288
Last seen: 5 days, 14 hours
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Re: Let's All Grow Mushrooms 2020 [Re: Jooby] 1
#26417118 - 01/05/20 07:25 AM (4 years, 24 days ago) |
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1/5/2020 welp looks like the aa+ and alt7 prints I had were not as clean as I was hoping they were, thankfully I swabbed a few plates so I hope I get something better on the others.

  looking like some sort of mold on the aa+
the pan cyan plates are looking clean tho, little bit of germ this morning
-------------------- Gettin back to work
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
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Quote:
Randalf the Grey said: Finally got my update in. Hope to get some lab work done.
January 4th, 2020
First signs of growth appeared in at least 1 plate of all varieties on January 3rd. Today, growth is apparent and growing well. APE is I little behind the others but I suppose that is to be expected.
I am trying to make it to my lab now to make the first transfers. Side note: I'm interested to see everyone's transfer plate numbering. That is, whether your spore\grain\tissue plate counts as T1 and first cut T2 or, first cut is T1. I have seen it discussed in here before but I am excited to see it play out in real time. I will only do 1 transfer of each variety tonight, and pour more plates. Then make some more transfer as I go, but keep those first transfer as the lead for this event.
Also have some tamps T2 to transfer (though not for this event) and will start some Z spores as a late addition for this event. I will try to consolidate my log so updates are not so lengthy, though I do tend to blather on about things of little import.
APE
 PFC
 PR
 CR (Coming soon)[/quote
hope they all turn out great for ya 👍
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A.k.a
Stranger


Registered: 10/27/19
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Re: Let's All Grow Mushrooms 2020 [Re: jcm4620]
#26417191 - 01/05/20 09:07 AM (4 years, 23 days ago) |
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After seeing some of these pics of how the very first stages of germination look on agar I think I might have some on a melmak plate. It looks kind of like little bubbles in the agar In some of the corners of the zig zag. Might just be from turning the swab though.
Guess I’ll know for sure by tomorrow.
I’m super jealous how quick some of these plates went off.
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LAGM2020     
Edited by A.k.a (01/05/20 09:11 AM)
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
#26417199 - 01/05/20 09:15 AM (4 years, 23 days ago) |
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Nikoyo see how that AA+ looks in a few days
--------------------
Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2020 [Re: ComebackKid] 1
#26417223 - 01/05/20 09:32 AM (4 years, 23 days ago) |
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JAN 5 I've got germination in one of my plates!
Potato phone quality photos 
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
#26417236 - 01/05/20 09:39 AM (4 years, 23 days ago) |
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Quote:
A.k.a said: After seeing some of these pics of how the very first stages of germination look on agar I think I might have some on a melmak plate. It looks kind of like little bubbles in the agar In some of the corners of the zig zag. Might just be from turning the swab though.
Guess I’ll know for sure by tomorrow.
I’m super jealous how quick some of these plates went off.
Its all luck of the draw. Spores are ALWAYS slow as hell for me. The spores from my RW print are exceptionally fast. 50 hours on my first try. I'm just going to bask in that for now as it's not the norm for me at all.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Let's All Grow Mushrooms 2020 [Re: Smartattack] 1
#26417240 - 01/05/20 09:41 AM (4 years, 23 days ago) |
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Still waiting here but should see something by tomorrow.. I had a late start
-------------------- Tek's I use LAGM2020
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 2 days, 16 hours
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Still waitin here, got some pooling but no growth I can see. At least there ain't no bacteria growing!
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
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Re: Let's All Grow Mushrooms 2020 [Re: Thufir_Hawat] 1
#26417318 - 01/05/20 10:40 AM (4 years, 23 days ago) |
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A.k.a
Stranger


Registered: 10/27/19
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
#26417365 - 01/05/20 10:58 AM (4 years, 23 days ago) |
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Quote:
Inthepit said: Idle hands are the Devil's Workshop! Well at least we've booked a flight back home now! Can't wait!
         In The Pit's LAGM 2020 GROW LOG
Ha ha! Shrooms!
Right??
I’ve been pouring plates just to do something. At least I’m prepared for the sea of plates I’ll be needing once these stupid spores pop.
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LAGM2020     
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit] 2
#26417372 - 01/05/20 11:01 AM (4 years, 23 days ago) |
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Prepping some swabs future use, thought I would go ahead and throw the link and photo of process up here. This may be useful told the end of this event for some of us.

Sterile Swabs Now by The Tribe
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A.k.a
Stranger


Registered: 10/27/19
Posts: 16,781
Loc: Gaming the system
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Oh nice thanks for posting that.
Def gonna be doing that myself, unless I suddenly grow fond of my loop.
Depends how these spore plates perform.
Is there any certain type of swab that works best?? I’ve seen a few sizes and densities.
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LAGM2020     
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
#26417405 - 01/05/20 11:20 AM (4 years, 23 days ago) |
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I just use regular ol' cheap ass no name qtips. In the Tek, it says synthetic and cotton both work but if cotton is used you should make sure they are dry before storing. Just leave it unwrapped in the SAB for a bit before rewrapping. I'm pretty sure the ones I use are cotton. You could cut them in half, but I leave them whole to give me a longer "handle". Just unwrap one end of the foil in an SAB, swab cap or spore, the rewrap or swab to agar.
Edit: PC just got up to temp and I realized I forgot to wrap jar lid with foli. jar might have a little more moisture in it, but I think it'll still be all right. We shall see. I can let it dry in my SAB if I need
Edited by Randalf the Grey (01/05/20 11:31 AM)
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,385
Loc: Terrapin station
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Re: Let's All Grow Mushrooms 2020 [Re: Nikoyo]
#26417428 - 01/05/20 11:38 AM (4 years, 23 days ago) |
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I use 12g agar to 500-750 ml. It's pretty hard and stuff. I like mine hard and stiff. Giggiddy! Do you guys think I'll have trouble germinating spores? Also, will the ape caps make a print?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,258
Loc: where?
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Re: Let's All Grow Mushrooms 2020 [Re: Hobbit GDF]
#26417437 - 01/05/20 11:44 AM (4 years, 23 days ago) |
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No they dont eject spores.
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
Posts: 678
Last seen: 6 months, 18 days
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Re: Let's All Grow Mushrooms 2020 [Re: trubblesome]
#26417498 - 01/05/20 12:35 PM (4 years, 23 days ago) |
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Quote:
trubblesome said:
Quote:
tealseal said:
Quote:
trubblesome said: oh wow yeah that's frustrating! back in the day when it was my job to pour plates we mixed at 20 g agar/L so I think you'll be alright
shit, i sometimes mix 50g to a L thats wat my jar says, i just been doing 25g to 500ml
I'm guessing you're using agar flakes then, which are typically instructed to mix at about twice the rate of agar powder
ya flakes, got the wrong shit a while back and just have been using it up deff going to be getting powder next time lol
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A.k.a
Stranger


Registered: 10/27/19
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Re: Let's All Grow Mushrooms 2020 [Re: tealseal]
#26417503 - 01/05/20 12:37 PM (4 years, 23 days ago) |
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That makes way more sense, I didn’t even know flakes were a thing.
I was thinking damn those plates must be like hockey pucks with 25+g in 500ml.
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LAGM2020     
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
Posts: 678
Last seen: 6 months, 18 days
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
#26417505 - 01/05/20 12:39 PM (4 years, 23 days ago) |
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Quote:
A.k.a said: That makes way more sense, I didn’t even know flakes were a thing.
I was thinking damn those plates must be like hockey pucks with 25+g in 500ml.
ya i guess i should have clarified a bit better lol, they are hard but not hockey puck hard lmfao
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Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 5 days, 23 hours
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Re: Let's All Grow Mushrooms 2020 [Re: Hobbit GDF]
#26417627 - 01/05/20 02:10 PM (4 years, 23 days ago) |
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Quote:
Hobbit GDF said: I use 12g agar to 500-750 ml. It's pretty hard and stuff. I like mine hard and stiff. Giggiddy! Do you guys think I'll have trouble germinating spores? Also, will the ape caps make a print?
The only time I've been able to print APE was when they dropped purple spores which would likely grow out like normal looking cubes. If they've ever dropped transparent ones I couldn't see any trace of them even after 24 hours printing. Swabs are your best shot but still may grow out normal looking.
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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