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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Let's All Grow Mushrooms 2020 [Re: tealseal]
#26416008 - 01/04/20 01:00 PM (4 years, 24 days ago) |
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I appreciate your posts. They're nice.
As far as a drop in the center goes, it works great IF the syringe is clean as fuck but doesn't qualify as "streaking". You're plates are looking good, man.
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Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 5 days, 23 hours
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Re: Let's All Grow Mushrooms 2020 [Re: tealseal] 1
#26416009 - 01/04/20 01:00 PM (4 years, 24 days ago) |
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Visible germination today from the 1st spores I ever printed 2 years ago. 
Pretty cool to breath new life into my 1st grow.
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 2 hours
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Re: Let's All Grow Mushrooms 2020 [Re: Grimsweeper]
#26416023 - 01/04/20 01:16 PM (4 years, 24 days ago) |
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01/04/2020: The Gym plates have exploded in growth compared to yesterday. 1

2

3

One of the Malaysian plates seems to have germinated as well (print was a few years old):
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Let's All Grow Mushrooms 2020 [Re: One of Us] 1
#26416041 - 01/04/20 01:22 PM (4 years, 24 days ago) |
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I'm apparently not going to have as much of a lead as I thought I might.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
Posts: 678
Last seen: 6 months, 18 days
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Re: Let's All Grow Mushrooms 2020 [Re: Smartattack]
#26416067 - 01/04/20 01:41 PM (4 years, 24 days ago) |
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i have been just adding a few drops from a syringe to a sterile swab and just swab the damn plates, they have enough condensation as it is i dont need shit rolling around on me, ive used a ghetto homemade loop too, but my swabs come pre-sterilized so thats just the way i do it
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Randalf the Grey
Woodland Creature


Registered: 06/11/19
Posts: 1,237
Last seen: 3 years, 3 months
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Re: Let's All Grow Mushrooms 2020 [Re: tealseal]
#26416076 - 01/04/20 01:49 PM (4 years, 24 days ago) |
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I usually just put 4 single drops at the 4 "corners" of the plate. In the process of wrapping the dish they move around a bit. I got sort of satellites at first instead of a smear or streak but seems to work well enough. I use a homemade loop to swipe prints but I see no reason to introduce 1 more vetor into the plates if I don't need to. Will update shortly with pics of growth.
Edited by Randalf the Grey (01/04/20 01:50 PM)
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 10 days, 10 hours
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I ain't got shit yet. Hopefully by tomorrow.
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A.k.a
Stranger


Registered: 10/27/19
Posts: 16,781
Loc: Gaming the system
Last seen: 12 hours, 36 minutes
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Re: Let's All Grow Mushrooms 2020 [Re: Asura]
#26416104 - 01/04/20 02:22 PM (4 years, 24 days ago) |
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Quote:
Asura said: I ain't got shit yet. Hopefully by tomorrow.
I’m with you. I hate doing things for the first time where you have to wait not knowing if it’ll even work.
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LAGM2020     
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 12 days, 11 hours
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
#26416117 - 01/04/20 02:32 PM (4 years, 24 days ago) |
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Ha ha! Them spores got a mind of their own!
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
#26416156 - 01/04/20 03:02 PM (4 years, 24 days ago) |
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nothing showing here either. i'll be offline for a week or so. hope to come back to something worth saving.
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the j stands for jesus.2020 new years grow along
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DcevLearn
Team Tomentose


Registered: 07/07/18
Posts: 330
Last seen: 4 months, 30 days
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You sound like me, no germination yet, tomorrow is my last lab day for over a week
-------------------- Current Project: LAGM: Stones From Scratch in 2020
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Colorblind-Myc



Registered: 12/16/19
Posts: 144
Loc: Rockies
Last seen: 5 months, 27 days
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Re: Let's All Grow Mushrooms 2020 [Re: Inthepit]
#26416161 - 01/04/20 03:05 PM (4 years, 24 days ago) |
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Both my plates have 4-5, 1mm fluffs going on! Much excite!
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
#26416196 - 01/04/20 03:22 PM (4 years, 24 days ago) |
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Quote:
A.k.a said:
Quote:
Asura said: I ain't got shit yet. Hopefully by tomorrow.
I’m with you. I hate doing things for the first time where you have to wait not knowing if it’ll even work.
I'm just praying there were even any spores at all in the cap that I swabbed I always have doubts with PE varieties just cause I can't see anything Loving the microscope shots in some of the grow logs I'm seeing. Making me want to get one myself
I should be getting some melmac spores in the mail any day now so I'll be streaking again soon anyway
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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-------------------- Tek's I use LAGM2020
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Re: Let's All Grow Mushrooms 2020 [Re: tealseal]
#26416275 - 01/04/20 04:26 PM (4 years, 24 days ago) |
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Quote:
verum subsequentis said: As far as a drop in the center goes, it works great IF the syringe is clean as fuck but doesn't qualify as "streaking".
Yeah, I should update the terminology. I guess I just see it as a similar mechanism - using the properties of a droplet to skew the present microorganisms by another physical means than a loop. I would assume (maybe incorrectly) that the bulk of the microbe load is deposited at the point in which the drop hits the media, and then when I tilt it about in a semi-controlled manner to kind of zig-zag the solution around, little satellite cultures have the freedom to germinate unimpeded by that microbe bulk. Something like 'droplet skewing'.Quote:
tealseal said: or u let it kinda just roll around the plate in a controlled manner after u place a drop?
That. Of course, adhering to tried and true cell-culturing techniques (like streaking) is a better way to ensure success. But it has worked for me. As verum said, a lot of that likely depends on the integrity of the primary source.
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One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 2 hours
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
#26416289 - 01/04/20 04:33 PM (4 years, 24 days ago) |
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I finally got around to swabbing the other woodlovers that I said I would do, plus an extra. For these I am utilizing some 2-compartment petris, with each compartment used as if it were a full plate. I am using MEA with 1.25% agar agar, after a failed attempt a month ago with 2% agar. The species I added to the party are:
Psilocybe azurescens (3 half-petris swabbed with swab left in fourth compartment) Psilocybe allenii (3 half-petris swabbed with swab left in fourth compartment) Psilocybe tasmaniana (7 half-petris swabbed with swab left in 8th) Psilocybe weraroa (7 half-petris swabbed with swab left in 8th)
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Jarhead3521
Grindin’



Registered: 09/26/19
Posts: 514
Loc: Amongst the pines
Last seen: 5 months, 21 days
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Re: Let's All Grow Mushrooms 2020 [Re: footpath]
#26416290 - 01/04/20 04:34 PM (4 years, 24 days ago) |
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So I added the link to my grow log, I think it works? Lemme know!
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trubblesome
Stranger



Registered: 11/09/19
Posts: 406
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
#26416579 - 01/04/20 07:38 PM (4 years, 24 days ago) |
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Got some stuff done tonight, details here: some single spore transfer attempts
highlight is this lone spore I found and transferred. goal is to workman method it with a big B+ print if it germinates.
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TheWitchfinder


Registered: 08/28/13
Posts: 76
Loc: Salem
Last seen: 3 years, 4 months
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Re: Let's All Grow Mushrooms 2020 [Re: Jarhead3521]
#26416583 - 01/04/20 07:42 PM (4 years, 24 days ago) |
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I'm going to make another batch of agar and pour again tomorrow. It's totally cool to use a print that you've previously scraped up, right? Also, I'm ditching using the Bobby pin as an inoculation loop and picking up some nicrome wire.
Also I remembered that I ordered a spore syringe sitting around. I happened to have a dish in my batch that I forgot to swab and appears to be clean, so I might try footpaths "drop & tilt" method for fun.
Edited by TheWitchfinder (01/04/20 07:43 PM)
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tealseal
spice of life ( o )( o )



Registered: 01/19/16
Posts: 678
Last seen: 6 months, 18 days
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Quote:
TheWitchfinder said: I'm going to make another batch of agar and pour again tomorrow. It's totally cool to use a print that you've previously scraped up, right? Also, I'm ditching using the Bobby pin as an inoculation loop and picking up some nicrome wire.
Also I remembered that I ordered a spore syringe sitting around. I happened to have a dish in my batch that I forgot to swab and appears to be clean, so I might try footpaths "drop & tilt" method for fun.
as long as u opened the print in ur SAB it should be fine man, u can use a print many times, just another option if u didnt wanna go the loop route, u can swab ur plates with a sterile qtip, mine come in pre packaged from a medical supply store down the road, but heres a tek on how to make ur own
STERILE SWABS NOW just an option
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