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OfflineHaywire
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Re: Let's All Grow Mushrooms 2020 [Re: SFS96] * 1
    #26388217 - 12/18/19 07:07 AM (4 years, 1 month ago)

I'm in!

cubensis:  Melmac & APE

6/1/20: spore swabs received from the Tribe are swiped on plates.

12/1/20: Melmac germinated (3/4 plates), APE not yet. Melmac first transfer is made.



17/01/20: I'm not expecting the ape to germinate on the plates. so made new agar 1/2 strength and added 3g/500 ml activated carbon. swiping plates today.

20/01/20: I've made more agar (6.5 g Agar | 7 g ME | pinch of graphite | 500ml water) and poured plates. Also did a hotpour of the first APE plates.



4/2/2020:

Neither the hot pour nor the first graphite plates showed growth.
In the second batch of graphite plates, I noticed a single colony growing which I then transfered to new plates.
Also, since the swab was falling apart after all that swabbing, I cut it off and burried it as per Fishlevelmidnight


so, yes, finally growth on the APE. transfered some wedges and monitoring now.

I also made a melmac liquid culture which is incubating now.


Edited by Haywire (02/04/20 02:19 AM)


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OfflineHaywire
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Re: Let's All Grow Mushrooms 2020 [Re: eatyualive]
    #26467844 - 02/04/20 02:26 AM (3 years, 11 months ago)

First post in this thread apart from the log but finally have something worthy of posting.
Got an APE swab from NAtedawg in the tribe giveaway but had trouble with germination.

first batch of regular agar. nothing.

second batch of half strenght agar with graphite. nothing.

then I poured a thing layer of the second batch agar on the first batch plates. nothing.

third batch of half strength agar with only a pinch of graphite. one colony, but possibly dodgy...

I also buried the swab head in the agar and then I finally got some potential good growth.



--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineHaywire
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Re: Let's All Grow Mushrooms 2020 [Re: A.k.a]
    #26469547 - 02/05/20 01:02 AM (3 years, 11 months ago)

Quote:

A.k.a said:
Why graphite?



because I didn't have activated carbon which should be a crude form of graphite.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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