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Multispore Enthusiast Registered: 05/27/16 Posts: 3,951 Loc: ked in the trunk |
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Start Date: Wednesday January 1st 2020
20 20 20 20 vision!! Okay let's get this going again!! We had so much fun last time. This really brought the community together back in 2017 and I cant think of a better time to get a community grow happening than 2020! If you're new to agar dont be shy! Sign up! Grab your supplies! Pleanty of time before the new year! Okay... A few rules! -All species and varieties welcome! -We will be starting from spores!! -ALL SPORES HAVE TO GO ON AGAR FIRST! -We start January 1st spores to agar -Add as much info as you can. This is a great learning tool for us and other users to follow along. "Mistakes" and all! -Post your updates in the thread as well as updating your OP to keep the conversation going - Have fun! Most importantly ![]() To Sign up Comment in this thread to reserve your spot. This comment will be your grow log. Keep updating it as we go along. I will link to your spot in the OP below Let me know the species and variety you plan on growing for the log! Sign up closes on Jan 1st 2020 I'll have a mod move this thread to mush cult on the 1st Let's see how many people we can get on board this year! ![]() Communal grow participants: A.k.a Species: Cubensis Var: Texas Orange Cap alaskappalachian Species: Cubensis Var: Wild Asura Species: ??? Var: ??? AyePlus Species: Cubensis Var: KSSS Bph Species: Cubensis Var: Purple Mystic Buckaroo Species: Cubensis Var: Corumba Buddaking Species: TBA Var: Camera93 Species: Cubensis Var: PESA Caps McGee Species: Cubensis Var: Purple Mystic CmbNorse Species: Cubensis, Pan Cyan CocaineBuffet Species: Cubensis Var: Texas Orange Cap Colorblind-Myc Species: Panaeolus Cinctulus Var: Warren ComebackKid Species: Cubensis Var: Albino + PBR cross Species: Pan cyan Var: Hawaii Species: Pan Cambo Var: Sandos Cootermonkey Species: Cubensis Var: Texas Orange Cap + PESA Crackatoa Species: Cubensis Var: ARX (APE x Redboy) cronicr Species: ??? Var: ??? curious.psychonaut Species: Cubensis Var: B+ DcevLearn Species: TBA Var: Durgin Species: Cubensis + Pan Cyan Var: PE + Hawaiian DnDRnD Species: Pan Cyan Jamaica, P. mexicana Jalisco, P. Cubensis PE6, P. Cyanescens South Africa, P. medullosa Estonia eatyualive Species: Cubensis Var: Stropharia eLeSDenes Species: Cubensis + Panaeolus Cyanescens Var: Rustywhyte (f10) + Jamaica (Asura) feldman114 Species: Cubensis Var: GT footpath Species: Cubensis Var: Chitwan fungi4us Species: Cubensis Var: Cambo, B+, Rustywhyte Greg Species: Cubensis Var: RW Grimsweeper Species: Cubensis Var: GT + Rustywhyte Haywire Species: ??? Var: ??? herbnmyc Species: Cubensis Var: APE + Mazatapec Hobbit GDF Species: Cubensis Var: Golden Halo Homegrow19 Species: Cubensis Var: Avery A + Melmac InfiniteDreams Species: Cubensis Var: Ecuador Inthepit Species: Cubensis Var: PESA J. Jack Flash Species: TBA Var: Jarhead3521 Species: Cubensis Var: Treasure Coast JessicaRabbit88 Species: Cubensis Var: Cam JohnnyKarate Species: Cubensis Var: RW GWM & Mystery print Johnny.rotton Species: Cubensis Var: B+ Jooby Species: Cubensis Var: Acadian Coast + RW Moose Mamma Species: TBA Var: MooseShroom Species: Cubensis Var: RustyWhyte mr. whothehell Species: Cubensis Var: Golden Teacher mushboy Species: Cubensis Var: Rustywhyte + Penis Envy Mycoactive Species: Psilocybe subaeruginosa natedawgnow Species: Pan Cyan nevereataaminita Species: Cubensis Var: Mars + Koh Samui (Classic) Nikoyo Species: Cubensis, Pan Cyan, Tampanensis Nobler Hino Species: P. Mexicana Var: Gandoli OericOss Species: Cubensis Var: TBBA One of Us Species: Cubensis var: malaysian P. Azurescens P. Allenii P. Tasmaniana Phycus Species: Cubensis Var: Purple Mystic PopaCap Species: Cubensis Var: Tasmanian + Envy Super Strain Quirkmeister92 Species: Cubensis + Tampanensis Var: Rustywhyte + Gandoli Randalf The Grey Species: Cubensis Var: SmurfHouse GT + APE Samiam76 Species: Cubensis Var: ??? Samskara92 Species: Cubensis Var: KSSS SFS96 Species: Cubensis Var: APE + Orissa India sh4d0ws Species: Cubensis Var: Ecuador shroomflow Species: Cubensis Var: Cambodian Gold + APE Smartattack Species: Cubensis Var: RustyWhyte spiritlands Species: Cubensis + Pan Var: Golden Halo, Red Down under sporealchemy Species: Cubensis Var: Melmak Staytrippy420 Species: Cubensis Var: Texas Orange Cap Svetaketu Species: TBA Var: Tattersail Species: Cubensis Var: PE TeaforTwo Species: Cubensis Var: PESA + Texas Orange Cap + Purple Mystics tealseal Species: Cubensis Var: ??? TheWitchfinder Species: Cubensis Var: AA+ Thufir_Hawat Species: Cubensis Var: PF Redspore + Leucistic Golden Teacher + Penis Verum Tookitooki Species: TBA Var: Tormato Species: Cubensis Var: ??? trubblesome Species: Cubensis Var: PESA Verum Subsequentis Species: Cubensis Var: ??? webweaver Species: Cubensis Var: Treasure Coast Yesum Species: Cubensis Var: CRS + PEU YogiBear Species: Cubensis Var: RustyWhyte PESA CONTESTS & PRIZES Durgin Grow log completion ComebackKid Grow log completion Grimsweeper First torn veil Randalf the Grey Print exchange/swap Sh4d0ws Supporter Status Edited by ComebackKid (01/19/20 12:18 PM)
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Multispore Enthusiast Registered: 05/27/16 Posts: 3,951 Loc: ked in the trunk |
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Okay I have an offer. I want to see everyones best work so...
One print or swab to any and all participants who complete their grow log. Log must include: -Dates -At least one documented agar transfer(s) -Picture of clean/healthy/desired mycelium -Bulk sub preparation -Inoculation process -Fruiting conditions (FC, GH, outdoor etc) -Mushrooms Upon completion message me with your choice of: -PE swab -ESS swab -Albino swab -KSSS swabI may update the list with more options down the road. There is no time limit. Even if it takes you all year to get clean mycelium on agar the offer still stands. -------------------- Substrate surface conditions / Monotub prep and care Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself Edited by ComebackKid (01/09/20 08:38 PM)
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ὕδωρχοίρος Registered: 07/16/19 Posts: 1,367 Last seen: 1 year, 2 months |
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• SAB: Sterilite 110qt • Petri Dishes: Nest 100mm x 15mm • Media Bottles: Cadamada 500mL • Agar: Now Agar Powder • LME: Breiss Light Dry Malt Extract • Knife: X-acto • Swabs: Cardinal Polyester • Torch: Bernzomatic WK2301 • Scalpel Handle: Swann Morton No.3 • Scalpel Blades: Swann Morton 10A • Jars: Ball Wide Mouth Quart • Lids: Ball Wide Mouth Leak-Proof PP5 Rye (10 quarts): • 11C Rye Berries • 14C water • 1T Gypsum Millet (10 quarts): • 15C Unhulled Millet • 20C water Liquid Culture: • 250mL water 1g LME Some sloppy technique in this video: - 0:47, I start monkey-gripping the syringe - hovering my hand over the sterilized needle, nullifying the sterilization. - 1:11, I have to pass off the swab from left to right hand - not a huge deal, but I should familiarize myself with doing it opposite (either streaking or dropping spores left-handed) I'll let the colony at 2 o'clock grow out a little bit and take it from there. That 5-6 o'clock range in that 2:30 colony looks best. Probably should have grabbed it a bit earlier. Fucked up a little by not taking all the way into the leading edge, and not quite into the 5 o'clock range of it. Whoops. I'll need to make up for my botched grab at that germ plate with another transfer or so. Nevertheless, It's nice to know I've got something familiar. Two of three look pretty enough for me to expand. I'll wait a couple days and take T2's, make some LC, and transfer some to grain. Might be diving in a little too deep for a first transfer but... That's why I took T2's.Edited by footpath (03/07/20 10:14 PM)
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Aum Mani Padme Hum Registered: 08/20/19 Posts: 1,742 Loc: Puerto Rico Last seen: 12 days, 11 hours |
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Stranger Registered: 11/09/19 Posts: 406 Last seen: 10 months, 16 days |
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Species: Cubensis Var: PESA ![]() 1/1/20 Here we go. I only did 2 plates (PESA print) because I'm a daredevil. but I also have a scope so I can verify the streak "worked." It did. Under the scope I found several locations where individual spores were just hanging out, so since I have a ton of relatively straight forward stuff going right now including a PESA plate about to go to grain and don't really NEED these to do anything in particular, I'm thinking about trying to do something fun with those and I'll be checking in on them daily. I'll use this grow as an opportunity to improve my microscope game as well. this lone floater down below that string of other spores is the most promising one I'm keeping an eye on, but there are some others too that I might be able to slice out and pop on another dish to see what happens. probably a foolhardy endeavor but hey. let's all grow mushrooms. 1/2/2020 and we have growth! bacterial growth. PESA 2 grew this little spot of bacteria fast, like 28 hours or so? yikes. but this is fun, this is one of my goals for 2020 - get better at microscope work. time to bust out the stains... 1/3 early AM microscopy edition the stain kit I got doesn't have crystal violet...I should have looked closer when I ordered but...come on. oh well. I'll wait to look at the bacterial growth, don't really have time to prep slides and shit tonight. we do, in fact have liftoff though none of the "loner" spores or any in the smaller groupings are doing anything yet. I'll check back on this tomorrow though, a leading edge from this could make for some fast shroomies. 1/3 I cut a diamond around that clump of spores, then sliced that diamond in half and put each half on it's own dish. 1/4: single spore transfer I found this guy on the PESA 2 plate that's contaminated. there wasn't another spore for a centimeter in either direction so I was confident I could pluck it and I did. This plate doesn't have too many spores on it anyway and that bacteria spot is growing rapidly so this will be my big experiment for the grow, I've wanted to do a PESA B+ cross but without obvious phenotypic differences it would be hard to verify what's happening UNLESS I can do what I've done here and isolate a single PESA spore (I'll see what F1 looks like and figure out how to deal with F2 later. problems for future me. if anyone has a thought here I'm all ears.). I'll print the biggest specimen from a B+ tub that was spawned 12/28 and do the workman cross method, scraping fresh spores in to a nearly completely colonized jar of grain from this monokaryote spore. Since a monokaryote can't produce fruits, the new spores from the print should link up with this mono mycelium, share DNA, and make fruits that have the DNA from both varieties! SO! How I did it: I found the spore by zeroing in on and scanning over my streak at 40x, (the max I can look at things on petri dishes under the scope, with different or thicker poured petris I can sometimes get 100x, but 40 is plenty for this). Then I lowered the condenser so that I could fit a sharpie underneath the plate. I used the light shining through from the condenser to draw a circle around where I was looking at the spore. Then I verified through the scope that this spore was the only one inside the circle. Then I cut a transfer out, working carefully to work just inside the sharpie line to be sure I didn't fuck it up. so here it is, a single god damn spore transferred to a new plate: I hope it germinates!! (I hope it's actually a spore too lol ) I actually had a contingency plan for this. I noticed directly on top of the sharpie line to the right of our star spore was another not quite as lonely spore, with a group of friends nearby. I figured I could get that one, and if I missed I'd end up with 2 anyway, then just for fun take the other 3 to a plate and have a 1, 2, and 3 spore plate. I took my slice and pulled it up on the scope, ready to celebrate my amazing scalpel skills. NOPE. I missed it by about a millimeter. oh well. now I have this "5 spore" plate that I'll take to grains/a shoebox if there's anything good looking that comes out of it. 5 spore plate ![]() The plates themselves. race plate transfer growth: ![]() 1/5/20 ![]() 1/8/20 on the 5th I made 2 jars of LC from both plates I'd transferred. I used the josex method more or less, though I used the tip of the needle like I was eating nachos and scooped up a nice large chunk of myc, just to make sure I had enough to get an LC started fast. If I had been more concerned about isolation or an even canopy or anything but speed I would not have done this. my method also differed in that rather than PC syringes with water in them to eject the myc poke, I sterilized a jar of RO water and drew that in to the syringes in my SAB, then flamed the needle again before performing the poke and squirt in to the LC. By last night it looked like enough myc had grown in the LC to do some jars so I decided to do 2 from each LC. Not what I'm looking for normally but it'll get the job done. There's millions of strands of myc in there. First I placed a drop of each LC on a slide for observation under the scope, and a drop each on a plate as a test. Normally I would wait until these test plates show no bacteria to spawn to grain regardless of what I see under the microscope. I looked at it live and did not see any bacteria, just blown apart strands of mycelium. this does not mean the culture is clean for sure, as we're really only doing this so that if we do see bacteria, we can toss them and start over rather than wait for the test plates to show what we already know. Anyway it was good enough for me to go ahead and noc the jars. I kept these 4 jars around specifically for this, as they were a bit old and quite dry, I figured they could handle some "extra" LC to make up for the relative weakness of the LC. Well, when I went to pour the first one I poured WAY too much. probably like 50 ml (somehow the dog hair remained in the jar). So I poured the next one normally but on the heavy side still, 10-15 ml. For the next LC I figured why not pour one of these heavy too so they can be friends? so one got 50 ml, the other 10-15. here they are: overpoured jars labeled "HEAVY" I also swabbed some grocery store mushrooms and put them to agar last night as well. they'll definitely need a cleaning up if anything does germinate: clockwise from top left: King Oyster, shimeji, maitake, white shimeji UPDATE 1/10 both my LC test plates came up clean, so yesterday I took the remainder of the LC, decanted most of the liquid off the top, and dumped about 15 ml of nicely developed snot balls of liquid myc on to two new jars, one for each LC. then I took a sample from the remainder and observed it under the scope just to verify it was still clean. It looked good, but I gram stained it anyway to help me better see any bacteria that might be present. while doing that I got this pic of what I think is a clamp connection at 400x: honestly I think the pours from yesterday will do better than the ones I poured too early, it's way more vigorous and is already showing signs of growth on the oats, the ones I poured too early are only just now showing growth. might have shot myself in the foot there trying to get a jump start! I also took a step back and realized that I'm probably taking on too much at once, and fucked around in my dishes too much to be actually tracking them waiting for a single spore to germ before contamination took over. so I swiped new dishes specifically for that project and I'll be growing nice looking transfers out like normal from the two transfers I already took. To be honest it's probably a waste of time and doing a fresh streak from whatever I harvest next will probably produce a single spore germination faster than the print I have. but I did it anyway because I was curious about trying out a weird method of spore application. it wasn't great but you know, not everything works: for the regular streaks I lightly drew my loop across some of the areas of my print with a finer spore load, then carefully turned the loop so that those spores were facing "up" on the loop. Then I streaked the plate with the bottom side of the loop touching the agar, so that the movement would more lightly draw spores off the top of the loop and increase my chances of finding single spore locations to cut from later. These were very sucessful, I found several locations with individual spores I'll be cutting out to move when I have time. The less successful plates, labeled "scatter," I lightly drew the loop on the spore print in the same manner, but then I held the loop about 4" above the plate, and used the tip of a flame sterilized scalpel to pull back the loop and then let go, the idea being a scatter of spores would be propelled on to the plates and I could find numerous locations with single spores in easy to pluck locations. I don't think I let the plate sit long enough before moving my hands and putting the lid back on for any spores except the larger clumps to land on the surface of the agar, and scanning the surface of the plates for spores that could be literally anywhere ended up not being very fun and kind of straining on the eyes so I'll be focusing on the light streaking method for now, it works fine enough. from the grocery store swabs, I have growth!! everything but the maitake germinated, which is what I guessed would happen. I don't even think there are any maitake spores on that plate. I'm working the maitake in a clone method from Tradd Cotter where it's dunked underwater with just the base exposed to force growth out of the soft tissue at the base. no spores. I made a separate journal for these where I go in to that in more detail: Growing grocery store mushrooms That's all for now - I think I'm at the point where I can just sit back and watch for a little while. UPDATE 1/13. SPAWN TO COIR. here we go I kept the sub a bit on the dry side for the "heavy" box since there was so much LC in there it's certainly got enough moisture. spawned to coco at 1:1.5 with a .5 casing, so I labeled it 1:2. gave the casing a good mist and closed them up. my last run like this I harvested the first flush yesterday after 15 days. if all goes according to plan, I'll have some PESA by month's end. UPDATE 1/16 Big day 4 single spore PESA plates, from a fresh print made and streaked sunday night. I had found 5 spores with a decent amount of space between them I was going to watch. When I went to check them today for germination I found one that had: So I got to it and made transfers of all 5. one of them I'm not 100% sure made the transfer so I won't include it here. I won't post them all here, but here is one as an example: I fucked up the cut on the germinated one unfortunately, and had to put it face down on the transfer plate. hopefully that doesn't fuck it up. It also prevented me from getting a good picture of it under the scope. I also did T1s of my king oyster, and T2s of my PESA destined for print making. I'll find one with a nice big cap and clone that and hopefully have a ton of nice big prints to make. Update 1/17: dog shit jars These jars are bad. but especially the one on the left: since they all came from the same LC there aint no way I'm spawning them to bulk. I might case the two on the right in the jar and put a baggy over the top just to see, but the one on the left gets the PC treatment. I'll give them the weekend and see how they look, but man they are dogshit, they are nasty. ![]() but that's why I did 2 LCs. The ones from PESA LC 2112 are still lookin good in their shoeboxes. Update for 1/20: I cased the better 2 of the dog shit jars. The other one was PC'd for 2 hours and dumped in the garden. blech. looked like aspergillus, which tracks with what I usually find on a contam'd petri around here. I had some previously hydrated coco sitting in a cooler. It was a little on the dry side but I put it on top of the jar and gave it a good misting, just til it turned a shade darker brown. here they are: It's not the end of the world if these don't work out. The big part of the update though is a tek I've been working on that I spawned my remaining PESA 2112 jar to (the good LC that is in my shoeboxes, not one of the dog shit jars) and was able to document the prep process for. I have some friends interested in mycology but they don't have the time, resources, or deep burning desire to make their own shit like those of us here at the shroomery might. So I thought about what a manageable way to share the beauty of growing your own mushrooms with friends might be, when I looked down at my take out container and thought it looked like the perfect size to make a little grow box out of. I tested it with some left over GT grain when I spawned a tub last month. So here it goes with documentation this time: Get some take out containers. you don't have to be picky, anything with a lid to retain moisture will do. Far left container was something a friend brought me some short ribs in a few weeks back My dinner tonight came in one of the others: 1 myco quart jar of spawn makes four containers. Which means you put a 1/4 myco quart of spawn in each one. These are oats: put about 1/2 a quart of the sub you like in there. this is straight coir after bod's coir tek.: mix it up real nice: I cased with a handful, or about a pint (1/2 quart) of coir: I generously misted the tops in the manner of a shoebox, as well as the inside of the lid. For delivery, keep the lid tight in case shit bounces around. When your friend receives the gift, tell them to lightly crack 2 opposite corners to get a little gas exchange going. These are small as hell and I'd be scared they'll dry out quick if they go right to the next phase without someone who knows what they're looking for watching them. You could also wait until they've colonized and give them away at that point if you'd like to make it even easier for them. Once the casing is colonized, they should take the lid off and put them in the plastic bag you delivered them in, give the inside of the bag a good mist, then tie it loosely and leave it somewhere with some ambient light that stays above 65F or so. A kitchen counter or something: For the most part, they should leave them the fuck alone. I opened the bag and gave it another mist every few days, whenever I noticed the condensation on the bag walls starting to go away. Last Thursday, 22 days from spawn, I had these little buddies: pins for flush 2 are coming in right now spot on. And of course the shoeboxes as posted above are going as planned. These are my 5th and 6th shoeboxes now and I think I'm finally getting the hang of them. ![]() GIVEAWAY![]() ![]() I haven't changed my status from tired in some time. I'm fucking tired y'all. Life sucks right now. But growing mushrooms with all of you and reading about everyone's projects and plans has been a major bright spot. Part of the reason I went for the race is that shit is about to get very hectic for me and in a little while I'll probably have to take a few months off from all this. If I do win the prints I'd like to do a giveaway to pay it forward. Life being the energy you put out in to the universe, not what you take and all that. I'll make a poll, LAGM growers can vote on a print from the contest bounty for me to grow. I'll do a shoebox at the end of summer, and I'll clone the best looking fruit from that and do a tub, make prints from it, and send those out to anyone who finishes a grow log by the end of 2020 and wants a print or two. Update 1/22 got pins trying something new to me this time. bags with holes on top to get that FAE/evap cycle right per the bod shoebox tek my bags are kind of wack though so I stretched them down as best I could my last few shoeboxes have had some kinda stunted growth/premature drying out/side pins so hopefully this and my more dialed in surface conditions improves the harvest. Update 1/24: I had some uh, questionable SAB sessions last week too. Did a ton of transfers last night for LAGM as well as my various other projects. Finally got caught up on some loose threads, hopefully cleaned up my SAB work after being really fucking lazy about it. Sorry about the pictures, fluctuating temps around here and not even 10 minutes with a hot coffee mug could get any of these clearer than what you see. Here's the LAGM stuff: T3s: When I tested my LC, culture 2111 had this perfect looking growth, completely uniform, I transferred it to a new plate to grow out for grain and...mold in the corner of the dish. fuck. so I took another transfer, we'll see how it does. honestly this might be something I do from now on. MS -> agar -> LC -> grains for tub -> tub, duh LC test plate growth -> isolate further -> grains for tub -> additional tub with less variety that is hopefully at least good, with an even pinset. The King Oyster got transferred. I made 2 transfers on accident, I think one will contam - a piece of coir somehow got inside of it. what the fuck. I grabbed another plate and after transferring noticed a piece of coir stuck to condensation inside the lid!! no clue. small piece left on the table that got drawn up by static electricity as I moved the plate near it? I s2g I cleaned this fucking table top... The Maitake swab plate started showing growth finally. Not knowing what Maitake mycelium looks like on agar, I was sure that it was going to turn green. But as the maitake clone grew out it looked similar, and both look like examples of maitake mycelium I've found online and what I've read about so what the hell why not! Not knowing which was better to take I made two transfers, one from strong cottony mycelium, and one from more whispy mycelium from further out, plus the one from the clone. I'll probably inoculate logs with it and bury them outside, but maybe I'll try one supplemented sawdust bag just for kicks. Trad Cotter details a method of progressively adding slits to bags that seems worth a shot. swab plate up without that pesky lid in the way: I expect these maitake transfers will need some extensive cleaning, but who knows, maybe that is all maitake mycelium in there. Update 1/25 shoeboxes today: "PESA 2112 1/9" spawned 1/13 "PESA 2112 HEAVY" also spawned 1/13 guessing I'll have torn veils monday night or tuesday morning. comparable to my first PESA shoebox from this print at ~15 days. question posed to thread: But I'm curious, is this just how PESA typically grows? or how shoeboxes typically grow? the first PESA shoebox I did had really skinny stems and the mushrooms themselves were quite short, they looked exactly like these only with a shitty pinset because it was my first shoebox and I let it dry out too much, so I thought the skinny stems were from that as well. I'm not sure if this is typical for PESA or if it's how shoebox grows typically look or if there's something I'm doing to them that I'm missing. They get misted whenever beads are just about gone but before myc is not dried out yet. They stay about 70 degrees, I removed the bags I posted about almost immediately after putting them on because they wouldn't stay standing up, and this morning I turned the lid slightly askew to give a little more FAE hoping to encourage some more stocky growth. If anyone has some insight to offer it'd be much appreciated. Update 1/26. Torn Veils: ![]() going to find a little better balance on misting/evaporating next time around, so much fuzzy foot going on. Update 1/27. Harvest day. Woke up to this: harvested the ones that were ready. They dried out to this when I got home from work: remember when I said the reckless nature of my speed run would show up in my results? There it is. 5.3 grams with 2/3rds to go. looking like a 15 gram haul from one quart of spawn. ![]() I think I know why they were so skinny/went so fast - today I moved the other tub on their shelf to give it a mist and noticed how warm the bottom was. The ambient temp of the closet they're in is 70-72, but the surface of the bottom shelf, which sits above the heat duct run through the ceiling below, was at 78 according to my IR thermometer. Shit looks like what I grew on PF cakes over the summer at similarly high temps. Only that time I did it on purpose because I was a noob and thought they needed to be pretty much roasting at all times. ![]() The other tub has more normal looking fruits, a more even pinset, should be done tomorrow (1 broken veil!), and I bet it will put out more weight. I'll do a final side by side when they're both done. The sub surface is confirmed by the IR thermometer to be at 72. Cobweb won the race in one of the top-cased jars: That's it! If anyone missed it, now that it's semi-official, I announced a giveaway in an earlier post, contingent on my getting to first torn veil: Quote: keep an eye out for that I guess! ![]() Edited by trubblesome (01/27/20 04:35 PM)
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Stranger Registered: 10/27/19 Posts: 16,781 Loc: Gaming the syste Last seen: 12 hours, 35 minutes |
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Sounds fun, this’ll be my first spore to agar grow.
I’ll be doing PESA, melmak, and TOC. Added AA+, rusty white, gt, z strain, pe6. 1-1-20 This is my first time putting spore to agar, it was a little tougher than I expected but that might just be because I’m not used to seeing nothing on the plate. I had a swab of Melmak which I had planned on using tweezers to pull some strands off and swipe them around but my tweezers were too big so I just ended up using the swab. For toc and Pesa I broke out my loop for the first time. I wasn’t sure if I was getting spores just using the loop so I torched it then cooled it in the agar then rubbed the print with it. I could see spores missing from the print this way so I felt better about it. Another thing I’m worried about is my agar mix. I was making low nute plates last week with the last of my premixed LMEA and saved some for today. However I forgot I had added extra agar for the first time to make it harder cuz I was having problems transferring the soft stuff. I tried to rough up the surface a little while cooling the loop then swiped the spores into that spot so hopefully it works out. Two plates of each Good luck everybody! 1/7/20 So far only bacteria on the melmak plate I stuck the swab in cuz I dropped it while cutting the end off. Just swiped another plate of TOC and PESA to softer agar. I’ve got three new prints coming anyday now so I’ll prob expanding my project cuz it’s some stuff I’ve been wanting to grow. 1/9/20 Had a pretty good size gt cap I printed and while I was about to fold it up decided what the hell and swiped a plate, I want to see how super fresh spores do. Later on the mail came and boom: Swiped two each of rw and aa+ Aa had so many spores I just scooped some up with the scalpel and smeared them. 1-11 Got germination on a toc plate from the seventh! Guess the softer agar helps. I’ve also got myc growing out of the extremely bacteria melmak swab. Took it and dabbed it a few times then buried it on a new plate. Gonna keep an eye on it and possibly keep it cold if the bacteria blows up until it outruns it. Looks like golden teacher and Pesa have also germinated. Finally in the game. Looks like agar makes a big difference, swiped from the same prints as Jan. 1 and had them go off in 3-4 days. Still waiting on the plates from the first. 1-12 Aa+ has joined the party. Just waiting on rusty now. Should be tomorrow cuz it’s a day behind the aa. 1-16 About ready for some T1s, just gotta pour more plates. I actually took 2 T1s from the dirty melmak swab plate on the 14th. Look good so far. Just got a z strain print and swiped that as well. 1-17 Forgot to add pe6, print came either 14 or 15th, and is kicking ass. Showed germination in under a day and like 30 hours in all five streaks I did have solid growth. Wish they all did that. I had some agar mishaps with the new bottles, got a few plates poured but not positive they’re clean. I did some T1s on aa+, gt, Pesa, and toc today so hopefully the plates are clean. I waited three days before using them so we’ll see. Can’t wait for all these new varieties. Still waiting on rw. Melmak seems to have waited out the bacteria and is blowing up with myc now, and the transfers from the other day are looking good. 1-18 Poured plates last night and redid T1s just in case the other plates aren’t clean. Just dropped a tiny piece from the toc germ plate into a little 200ml lme lc. Gonna try a little of everything with all these things. I also started putting two T1 transfers on each plate to save time and supplies. Now that I’ve been doing agar long enough to go through the whole process I’m realizing T1s are pretty useless and am gonna streamline things to get to t2/3 ASAP. 1-22 Took t2s from Pesa, toc, aa, pe6, and melmak. The lc is starting to blow up everytime I look the myc floaters are bigger. Been shaking it violently trying to break myc off the wedge. Also knocked the first round of T1s and fresh 2s onto the floor, so that sketchy agar saved me cuz I had backups for most of them. Had pin mold on one aa plate for sure, possibly both but just starting if it’s on the second one. Took a t1 from the z germ plate. 1-23 Toc lc, almost five days in About to do some Pesa and z transfers. 1-25 Took a few more transfers, t2 and 3s, they looked pretty good so went to one wedge per plate to grow out for grains. Made an attempt at my first lc pour with the toc....it went...questionably lol. The wbs was wetter than I planned and instead of pouring off some water first to make the lc thicker I just went for it figuring since idk what I’m doing yet might as well spread it out. Poured about 50ml which made the jar waaay wetter than I thought it would. Gonna keep shaking it up to try and distribute the water until it dries up. I’m gonna make another jar but make sure it’s a lot drier this time. 1-27 Did a ton of transfers today. A couple t2s and a bunch of t3s to really soft plates that hopefully will be going to grain. Reswiped rw, fourth plate of it I just can’t get it to go. Totally unrelated but also swiped a pan cyan print I recently acquired, pretty excited about another species. Hoping to see some growth on my toc lc jars tomorrow or the next day. 1/28 Starting to see fuzzy spots on the toc lc jars. Dropped a little plate of melmak into wbs. Got two more lc bottles fresh out of the pc cooling down, prob going with pesa and aa. 1-29 Haven’t posted pics in a while so here’s some of my latest plates, got a few newer ones but no growth yet. I’m so happy with the melmak. 1-31 Made z strain and pe6 lc today. Seeing an exciting amount of growth in the toc jars I poured. Not part of LAGM but my pan jam spores germinated yesterday as well. 2/3 Forgot to update but did more transfers and dropped some aa+ into a quarter filled jar for g2g. Toc jars are totally covered but the myc is thin still, I think from being too wet. My aa lc went bad but the others are good. Here’s pesa a week in. 2/6 Spawned one of my toc jars just now. Finally at the fun part. Idk what’s going on lately but my agar has been a disaster, last two batches I poured stayed liquid and I even used more than usual. Shook my aa+ jar the other day it came back real nice should be ready to g2g in a few days. Just bought a 40lb bag of wbs, usually I go for the walmart economy bag but saw this southwest blend that has mostly small seeds like millet so decided to give it a shot. I also dropped all but one wedge of this melmak into a jar for a master. Z, pesa and pe6 lcs all looking good. 2/9 Split my quarter quart of aa into 4 quarts, they’re gonna go into an under bed tub. Shook up a 10 day old melmak jar that was healthy but so slow. 2/11 Aa+ g2g less than two days in. Both toc tubs seem to be coming along, I’m curious how they’ll do cuz the spawn was way too wet from all the lc, but it was pretty thick and came back right away after a shake. 2/12 Toc five days in Other one looks similar. I’m planning on pouring some lc today, probably pesa, pe6 and z, none of which I’ve grained yet but have good plates for too. Poured 2 jars of pesa lc, only like 10ml this time to drier wbs. The other ones were looking iffy so gonna test them first. Finally dropped a z plate to grain. 2/13/20 Toc from lc at six days. 2/19/20 I can’t believe it’s the 19th already. Ok so the two pesa jars I poured lc into are looking good, the z jar I buried the wedge dead center where I can’t see it but there’s myc poking out all over now. The aa+ I g2g into 4 quarts...one is super healthy and done, 1 looks pretty good, 2 are questionable. I used a pe6 plate made super soft so it would liquidate during shaking, and that’s working amazingly. The first melmak jar I made three weeks ago is 99%, but the master I made from the green agar plate looks like it’s not coming back from the shake. 2/23/20 Toc pins popped up the other day, my first spore to shrooms grow. Spawning pe6 and melmak hopefully tomorrow, melmak s beem ready for days. Just dropped a t4 plate of aa+ that looked great into grain and g2g another jar from the best aa+ that finished few days back. Both to replace the questionable jars meant to go in the big tub. 2/23/20 Spawned melmak, pe6 needed another day. 2/26/20 Spawned pe6 yesterday. Here’s the toc that’s doing well. 2/27/20 Annnnd my first agar spore grow is almost complete! Normally I’d harvest in a few hours but since this is my first run I gotta take a bunch of prints. Z and pesa and probably aa that didn’t make the cut for the big tub should be getting spawned tomorrow. Just cut off a handful of caps and set them up to print before bed. Too lazy to harvest now but had to take a couple off before they opened up. 2/28/20 About 345 wet 2/29/20 Spawned z and pesa. The pesa was iffy, I’ve been having a lot of bacteria problems since switching seed brands for some reason. I shook up the other jar of pesa a few days ago and it’s coming back but not looking great. Also lost a couple aa+ jars so I had to g2g another one and dropped another plate a few days ago. I really want to get my big tub going! 3/3/20 Second toc came in as mostly one giant cluster. I’ve got a few clones to test out now between the two toc tubs, pretty cool. 3/11/20 Finally spawned a shoebox and underbed of aa+. 2 and 3 quarts so smaller underbed unfortunately. Z pe6 and melmak are knotting, pesa should be also anytime now. Totally forgot about the golden teacher print I took, dropped that to grain few days ago it’s looking super healthy. That’ll be my first grow from my own prints. 3/13/20 Harvested some second flush toc, and top cased/coir mugged a little sketchy melmak spawn I made with the green plate a while back. Pesa 3/16/20 Gt ms jar, first grow from my own print. Pe6 in the home stretch. Definitely clusters like toc, but caps are brown. 3/17/20 Pe6 shot up in the last twelve hours. Melmak has got some little clusters forming too. 3/20/20 Melmak pins been sucking up water, they’re dry every time I check now. Harvested and printed half of the z. The aa+ underbed ran the coir and started knotting on day 7. I spawned another two pesa, one from a plate and another from the same lc as the first tub which is 19 days in with no knots. Also spawned my gt jar from the giant cap print I made. 3/22/20 Melmak ending up way smaller and totally different looking than expected. Aa+ underbed pinning at 10 days! 3/23/20 Melmak Aa+ about 11 days 3/26/20 14.5 days. The first PESA finally has 3 pins. ![]() The gt shoebox is suuuper healthy, just about hitting 100. The other two pesa spawned the same time are prob like 70%. For some reason the pesa jar that consolidated for a week or so while I waited for more to finish is so much faster than the others. Might have to experiment with that. 3/27/20 The aa+ shoebox I made with two quarts of bacterial spawn has some nice clusters forming but pretty small so far. The gt is looking beautiful and about to start knotting. Underbed tub hit the growth spurt overnight Some second flush pe6, looking potent. And my growroom buddy hogging the heater 3/28/20 Harvested aa+ this morning, first load dried pretty heavy 44g. Gonna guess about 70g total first flush. 3/29/30 Harvested more pe6 and z. Second flush of melmak coming in quick, prob ready in two days. This is pesa from a plate, looking much better than the first one. I’ve dont Think I’ve ever had this mixed myc before. 4/1/20 Harvested second flush melmak, z and pe6. Plus the bacteria aa+ shoebox which was like 380 wet all small shrooms. Melmak My two toc clones are prob 70% colonized, can’t wait to spawn those. 4/7/20 Spawned the first clone from my toc and a sketchy looking jar of playa uvita yesterday. This morning I harvested a bunch of random later flushes. This is mostly z with two pe6 in the back near the scale. Jar of melmak I cased is going off too. This is z. Pe6 My gt has a bunch of pins, Both pesa have started pinning, the old pesa has been steadily putting out 3-4 inch shrooms with toothpick stems. The big aa+ tub doesn’t want to flush again for whatever reason. A few on the edges are there but other than that there’s just now a couple pins popping. Finally got pans figured out enough to stop frying pins. So stoked on these. I’m swiping tamp and atl today, doing a ton of plate work and hopefully graining ufo, Amazon, rw, taz and spores I swabbed from my PE clone. Not all lagm related but whatever. This wbs is killing me. Everything will look awesome then I’ll shake and half the jars go really bad. Like the grain gets all dark and wet. I’ve lost four awesome plates this week to it. 2 ape, a melmak and a Burma. 4/8/20 Melmak jar and gt ms from the huge print Both the new pesa shoes are pinning, and the older one is steadily putting out skinny ones. 4/9/20 Harvested the ms gt. Nothing special, about 270. There’s some big aa+ Sidepins on the underbed tub and second flush pins are finally coming in but there’s also a little mold on one part. Not trich though it’s this really slow moving stuff so it should be fine. 4/13/20 Harvested 5 kinds of late flush lagm varieties. Aa+ underbed put out another 500ish. Plus the longest shroom I’ve grown. Plus this tiny melmak pair. All three pesa are just slowly putting out a few tiny shrooms each day, pretty lame. Both toc cluster clones are killing it, one was knotting at day 6 yesterday. Dropped ufo and ape to grain couple days ago. Hopefully they work this is my 3rd ape attempt. Used up the last of my stupid southwest wbs too. 4/20/20 Been a minute since I updated. First off here’s my two toc cluster clones spawned on 4/6 and 4/9 My three pesa tubs are still weird but finally starting to put out more normal size shrooms. Got my biggest ever shroom from that PE6 tub spawned 54 days from this pic. Playa uvita is super slow but looking healthy. Almost colonized the tub at like two weeks. I switched a bunch of jars to sfd and every single one went green by 10 days in. Just redid the lids and ran a batch last night, shook them up and now I’m waiting for them to soak up the moisture. I’ve finally got a jar of pfc that looks good, just shook it. Also have another toc lc jar and one of ufo. So far both tubs from the toc lc grew crazy clusters so I’m hoping for more. 4/26/20 Oook. So my jar problems continued. Ufo is the only one left and I can’t tell if it’s super slow or bacterial. Replaced all the filters with tyvek and just knocked up amazon, 2 ufo, ape—again, a melmak cluster clone and plate pin, and some ms PE from my big clone tubs. I took two clones from big toc clusters, one went decent and one looks good but yielded shit. Here they are along with a pesa from the hc. I also poured the last of my toc lc into a jar a few days ago, nothing yet. It’s getting super hot out already and I’m gonna need to bring in the ac or switch rooms. The playa uvita took 19 days but finally pinned. Also just finished my second pans grow which went beautifully. 4/28/20 Picked a few more big pe6, they jsut keep coming. Idk what happened with the gt but that’s 2nd flush, might be the big genetics it came from or that there’s only a couple shrooms. And playa uvita making progress. I knocked up 8 more jars two days ago. Hopefully these stay good now that it’s back to tyvek. I prepped the seeds a little different and I seem to be getting real quick jump off so it might be my new prep. 4/30/20 Man I can’t believe it’s been five months already. I’m gonna have to move everything across the house to a cooler room cuz it’s breaking 90 inside during the day and last night the low was 77. Plus it’s so brutal doing SAB work in the heat I just keep putting it off. Anyway my bunch of jars are all doing great, no signs of mold finally. Plus my toc lc I thought was dead is finally growing all over the jar in tiny puffs. Playa took off overnight, solid stems. Printing caps now and might clone the tall one. One of my toc clones had like three different contams on one end so I cut a third of the sub off. I hope it works cuz there’s a lot of pins still. 5/3/20 Spawned ufo this morning finally. Can’t wait for this one. Both toc clones got mold during the second flush, so many contams lately I’m gonna do a serious clean. Playa finished first flush and I took a bunch of nice dark prints. It was staggered but I think I got around 25g dry. Tall skinny shrooms with cool caps, and the stems are rock solid. My jar of pans got mold on top so filter must be bad. The rest are looking good though, shook a couple and the toc lc spawn jar finally started growing. 5/8/20 Harvest more playa, prob up to 35g dry now. Also the last pesa triched out. All three shoeboxes were super weird but I probably got at least 4oz dry. My ufo is colonizing the coir nicely. All my spawn jars look awesome, such a relief. Dropped two rusty whyte pins into a jar this morning and transferred the third one to another plate. Also made a ufo pin clone plate. My ms gt is putting out some bigger ones now. This is about third flush. Also spawned pearl oysters to the sub from the aa+ shoebox this morning. First run at edibles. 5/12/20 So boring now that I only have one tub going since I lost so much spawn. On the bright side a bunch of jars should be done in a day or two. I’m gonna g2g most of them though so it’ll still be probably 10 days minimum. Although I have another jar of ufo that’ll be ready for a shoebox hopefully by tomorrow or the next day. Here’s my current ufo as of yesterday And a jar of PE getting g2g tomorrow probably I have amazon and Tasmanian jars almost done but I’m going to g2g them to 4-5 quarts each for underbeds. The jar that got the last of my toc lc is also almost done and getting g2g. I’m gonna put some grains from it to a plate because the two tubs it made before had insane clusters so I’d like to keep it around. 5/23 I’ve been slacking on this. Ok so the toc and PE g2gs are almost done. One of the toc got a spot of mold so it’s gonna be shoeboxed. My first ufo box got a pin at day 17 or 18, taking forever. Spawned another box like a week ago. I also spawned the Amazonian and taz jars instead of expanding them cuz I’m bored and want grows. I made another half full jar of Amazonian spawn to expand and I have a good taz pin clone plate to do the same with. This Amazonian is the fastest culture I’ve ever had. Knotting on day five. This was two days. Finally about to spawn a sketchy jar of ape too. Edited by A.k.a (05/23/20 08:56 AM)
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don't fear the sweeper Registered: 01/29/18 Posts: 4,843 Loc: broom closet Last seen: 5 days, 23 hours |
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GRIMSWEEPER LAGM 2020 GROW LOG Also a Pan Cyan Trop I haven't been able to germinate since I love a challenge. Species: P. Cubensis Variety: Golden Teacher RustyWhyte Species: Pan Cyan Variety: Tropicalis 1/1/20 - Only had condensated plates sitting around but here we go, off to the races. Happy New Year and good luck everyone! Edit 1/2/20 I received 2 prints in the mail today I'm kinda excited about so I'm lumping them in. Sreaked on 1/2/20 Species: Pan Cyan Variety: Wild East Coast South African Species: Psilocybe Natalensis Variety: "Super Strength" South African UPDATE 1/6/20 T1's takes on - RW South African Pan Cyan Natal GT South African P Cyan had bacteria on the germ plate all others appeared clean. UPDATE 1/8/20 - Started my grain prep hoping 1st transfers are clean enough to go to grain in a few days. Based on how clean looking my germ plates are I think theres a good shot at T1's going to grain. UPDATE 1/14/2020 RW and GT T1's going to rye today. Not as uniform as I usually like but the germ plates still look clean and I'm going on vacation this week for 7 days so fuck it. Natalensis and Pan Cyan will get another transfer. UPDATE 1/24/2020 RW got it's 1st shake today at 20-25%. GT is moving slow so it'll be another week or so to shake those. UPDATE 1/28/2020 RW 4 days after 1st shake GT ready for their 1st shake Natalensis plate is far beyond ready but haven't had time for transfers. I may make a 1/2 pint master from it. UPDATE - 1/29/2020 Normally I'd take another transfer of this Natalensis but I'm feeling lazy and experimental-ish. I prepped a little too much spawn yesterday so instead of tossing the grain or PC'ing the extra qt or two I'm trying 1/2 pint masters. Aren't they adorable? ![]() Instead of transferring clean growth to a new plate just to grow it out I'm transferring to a 1/2 pint master. If all looks good I'll expand to quarts. I have 6-8 other cultures that have just been sitting in my fridge so I'll master jar a few of those as well. Edited by Grimsweeper (01/29/20 08:16 AM)
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a dojo and a forge?! Registered: 08/29/15 Posts: 1,774 Loc: Deep Ellum |
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I'm definitely in. Species- Var- galindoi (I learned later that this is actually a tampanesis grow) Hopefully I'll produce some fruits, I've gotten plenty of stones on many occasions but never a mush. Let's see if I can break the curse. ~~~cbk~~~ I had the opposite problem ![]() Changa alchemist did a very good write up somewhere I cant find right now. I think he did a 50/50 grain/coir mixture in a shallow tub if I remember correctly That's interesting man. The reason I included "galindoi" as the variety is because the print I received was actually labeled "ps.galindoi". Galindoi is just So I think I'll use Ham's rye seed tek for this species as they are grass lovers. Happy New year's! 2020 is gonna be my fuggin year! Jan 1 Swabbed to brf agar Jan 9 Made my first transfers. This is a shitty pic, I took some better ones I'll post soon. Jan 20 On my second transfer now, plates look great. Here's where I transferred from. I also threw a wedge on some wbs for kicks (that jar bit the dust quick) Jan 29 On my third transfer now (Mexicana). Plates look decent, a couple looked quite fluffy for my liking so i made some biopsy pokes to agar in addition to the pf pucks I made a few days ago. Not the proper size of needle but it's been working great lately. Feb 9 So I have some nice biopsy plates and pucks to play with. I already used one chunk from a puck to make a little slurry and poured to 50/50 coir and rye seed. Some were inoculated with a LI made from a plate (not the biopsy). I'm confident in the biopsy transfers I just made too so I should have a nice foothold on this grow. Those are the biopsy plates I transferred from and a pf puck I'm growing out. (Those bottles bit the dust) Feb 22 Just made some pint jars of oats inoculated with a plate on the third transfer. Looked really nice and covered the whole plate and both the LI and the plate smelled like crushed stones so I'm hoping I got this one right. If this run doesn't work I have like 20 nice plates now ready to drop to individual quarts instead of using one as an LI Mar 4 So my last batch bit the dust too, that what I get for rushing into liquid inoculants. But keep an eye out for my trap door tek for loading syringes without an injector port. Oats have tiger drops and the lil bottles are rye and coir with some brf sprinkles and noc'd with a biopsy plate ![]() Mar 12 Finally getting some nice growth post shake. What is it, almost 3 months in lol. I love seeing people Duke it out with contams, especially after seeing nothing but success pictures on this site. This thread proves that this hobby requires hard work and patience, nice work everyone. I'm right behind you lol. I'm about to drop five more plates from the 4th transfer to some wbs and rye grass seed mix. The lil bottles are looking fine, slow growing compared to the oats. Mar 21 Finally got some stones starting in an oat jar. The mycelium looks shitty but seems to not mind. Mar 27 Starting to get some nice lil stones in those bottles, here's one saying "what up" Apr 7 Finally got my first mexi-pins ever Apr 10 Bout to do a little cloning These came from a little bottle that tammed its interesting seeing how different the mushrooms look. the fat pins are the coir and rye seed caks and the tall skinny ones are cased grain. Edited by Nobler Hino (04/11/20 06:09 PM)
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stranger than ever. Registered: 11/20/13 Posts: 1,500 |
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outstanding community project. many thanks.
my contribution: new decade, new species first semperviva project bonus: psilocybe cubensis rustywhyte fourth rustywhyte project, but with a twist. details of my foolishness to come. second attempt to germinate hoogs. first try, nothing happened. today, three plates each from two different prints. first try swabbing prints myself. using asura's streak technique. 20200101 - rustywhytes the setup ok, my foolishness. i've run three rustywhyte projects to date. first two, multispore from separate prints, both came out very not-fully-rustywhyte. pretty cool, but not what we were hoping for. i cloned a fruit from this box, and ran the third project, which resulted in a fully rustywhyte tub. unfortunately, this is the only foto i have of it. it turned about as well as any cube project i've ever run, which hasn't been many. this print came from that tub. around the time of the mixed up shoebox above, a beloved member sent me another rustywhyte print out of sympathy. so three plates each from those two prints. well, another beloved member's been conversating much lately about crossing strains. so i done up three more plates double streaked with the prior two swabs, theoretically crossing rustywhyte with itself. the idea, fruit them all and see what happens. i know from experience, expectations are for the birds. i can't emphasize enough that i don't know what i'm doing. germ plates aren't far along after two weeks and change. some t1 transfers made none from the nameko print germinated. the other generally fared much better. 20200116 - rustywhytes germination not as vibrant as i'd hoped. all in all, very clean, but not prolific. this particular one of the crossed plates had the densest sections, where i'd guess it most likely to have succeeded. all the transfers. first transfers doing their thing. these looked kinda fucked, but it's just the ignorant wretch poorly pouring plates. germ plates. all from the same print. the plates from the other, older print are still as clean as the day they were poured. 20200124 - rustywhytes about time to make second transfers and prep some grain. below, t1s next to the germ plates. j, from the print from the mosaic box. s, from shaper's gift. x, the attempted cross. for grins, the san pedro cacti i set to germinate i can't remember how long ago. fucking adorable. 20200126 - all projects. second transfers made. no fotos. we all know what it looks like. the following mostly went to grain, half plate to one jar, minus one failure. ended up with seven jars hoogs. 20200209 rustywhyte put all of these to grain, half plate to one jar. that's twelve jars. left to right: rwj, rws, rwx just prior to shake this stuff is beautiful. 20200220 rustywhytes just prior to shake. several jars not pictured. one jar wasn't ready to shake, having barely grown out at all. not a fan of these new lids. shame it seems they stopped making the white ones. Edited by J. Jack Flash (02/20/20 10:54 AM)
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Deadhead Registered: 02/14/19 Posts: 3,385 Loc: Terrapin station |
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I wanna play. I got tribe giveaway print.
Species: cubensis Variety:Golden Halo & Rusty Whyte & TAILFEATHERS (wife's) My wife wants to try her hand and she will be growing along side me. She is choosing the cube variety TAILFEATHERS. January 1st 2020- my wife and I swabbed our plates. We used the flowhood I built. We swabbed two plates per variety. Again, my varieties are Golden Halo and Rusty whyte. My wife's variety is TAILFEATHERS. The agar was made with potato flakes and honey. There are two kinds. Pink and purple. We swabbed one of each per variety. The pink is just potato flakes and honey The purple is the same with added Peptone G. I think it will be good to see how the myc likes the pep. I will continue to make both types of agar till it's time to put to grain. -jan 4th~ no signs of growth. One of the Golden Halo plates was pure bacteria. 4 out of 30 transfers of other projects had it also. That being the case I swabbed 3 more plates with GH😇. Will post pics once germination. ![]() Jan 6th~ so after work I looked and Bam! We got growth. The one looks like bacteria. That is golden Halo myc right? The green plate is the one I swabbed few days after the 1st. There is a little growth so I transfered both plates and made 2 more per plate. I think TAILFEATHERS is showing signs of germination also. I hope Rusty Whyte does too soon. ![]() Jan 10th~ I did some transfers of Golden Halo, RustyWhyte, and Tailfeathers!I labeled the 1.9 obviously because I thought it was the 9th. I tried to do two per good plate. I'm so excited. I can't wait to put these varieties to grains. Hopefully they will grow out very clean. I've been getting bacteria in about 2 or 3 out of like 30 or 40. Stay tuned!! ![]() Jan 15th~ so I didn't take to many pictures. I transferred multiple plates per chosen plate of previous transfer. The agar I've made recently has been very nutritious. Im trying to get lower nute agar sorted out. Last run of plates are just grain water and agar. I'm just now getting into them. So the golden Halo are on the 3t now. RustyWhyte and Tailfeathers are one behind them. I used Tailfeathers to A2G and big pickle jar of oats. I also did A2G Golden Halo to 1qt of oats. That's all oats I had. I need to get more grain. It's hard to tell where to transfer from since there so much nutes. We'll see what happens. ![]() Jan 27th~ I've made quite a few transfers of best plates. All the myc has been super thin and tomotose and almost invisible. My agar has had to many nutes lately. Ive changed that.I also did some A2G with RustyWhyte, Golden Halo and Tailfeathers. 3 qts of oats each variety. The big pickle jar of Tailfeathers has been through one shake. Here's how it looks right now. It's growing fast. I'm excited to see it progress. The first qt of Golden Halo is also doing great so far. I already shuck the jar once. This isn't as fast as Tailfeathers but it's about the same. I'll try to take more pictures. Journal better. ![]() Feb 2nd~ I spawned the Tailfeathers and Golden Halo today.The TF went to a 58qt mono and the GH went to a shoebox. The sub is coir & verm. Cased with same thing at spawn. TF GH I have other jars of all 3 varieties colonizing now. RustyWhyte looks the thinnest and slowest so far. Feb 8th~ this is my golden Halo shoebox. The Tailfeathers mono is not nearly this far along. RustyWhyte are still colonizing grains. Which I just shook. I have ³ of each variety on oats. Trying rye out too. But here's GH sb Feb 16~ so the golden Halo is starting to pin. Tailfeathers is pinning really good. 10-20% are white. That must be the AA+ in the mix. I also had so GH pins in a plate. I cloned two. One was like whiteish looking and the other was pale but normal.I'm going to clone the white Tailfeathers and print. Ill post pics soon. Phones broke and dont charge. Feb 17~ here are some crappy pics. Tailfeathers is doing some colorful stuff.Golden Halo is pinning.. Feb 18~ here are how they look this morning.Tailfeathers Golden Halo Here's my first PE clone tub that I executed perfect jiffy casing. It's starting to pin really good. Feb 19~ harvested these last night.I took 4 prints of the white & normal. Took clones of 2 white And 1 regular. I'll add in dry weight tonight. I want to stabilize the white phenotype. Would that just be AA+ or would it be all 3 in one? (AA+, corumba Brazil, and Golden teacher mustang "cluster fuck". Feb 19~ so the Tailfeathers weighed 70 + dry. Not bad for big pickle jar not all way full and MS on T1.Here is my golden Halo SB . They look funny and mutating a little. Fat tho. Feb 20~ here's what the golden Halo shoebox looks like. Good clone canidates. Here is another tub. It's a clone. Idk what variety. It was supposed to be penis envy. clearly not. Looks good. Hope it's not going to be small. See the stripes on the stem? Isn't that sign it's to wet? Feb 21st~ here is my mystery cube clone.I am about to harvest and take prints. Also printed some white Tailfeathers (WTF) and regular Tailfeathers. I'm about to do a giveaway this weekend. I also harvested the golden Halo SB. I didn't take prints but I did clone 3 of them. I figured I'd grow out that then take prints for you guys. I love the gold spores. Looks pretty than purple blackish mess. Well I'll let y'all know dry weight later. Ok so dry weight of golden Halo shoebox is 20.13g The unknown clone mono was 70.56g dry (⁴qts spawn) April fools 1st. ~ ok so finally I have my first real rustywhyte mushrooms. I'm stoked that there is the one huge guy in a cluster. Nice clone candidate. I was low on shoeboxes at the time and the spawn was sketchy so I just threw some sub and spawn in this grow bag April 2nd - here are some apes. One tub is clone the other is from spores from the clones . I'm Make some swabs tomorrow. Harvested some golden halo, costa rican and some rusty whyte today. Took prints of RW & GH. I look forward to seeing the difference in their prints given they are rusty/gold not black/purple 5/30/20- finally got some nice rustywhyte. 7-3-20: here are some of my 2020 grows so far. Thank you shroomites and shroomery. Edited by Hobbit GDF (07/03/20 07:27 AM)
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don't fear the sweeper Registered: 01/29/18 Posts: 4,843 Loc: broom closet Last seen: 5 days, 23 hours |
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I'm the one who mentioned a contest so I'll put it out there. I pledge a 10 pack of prints/swabs for 1st torn veil of harvest size (not a tiny mutant).
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ὕδωρχοίρος Registered: 07/16/19 Posts: 1,367 Last seen: 1 year, 2 months |
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I'm not of the competitive variety, so if I happen to 'win', please offer it to the next in line.
Those ESS look do look pretty though... OP updated with:
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Eat's You Alive :) Registered: 08/17/01 Posts: 19,026 Loc: In Your Head |
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This is a fine opportunity for a spore print pool! And seriously someone has to photoshop all the print pictures into a pool.
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Stranger Registered: 10/27/19 Posts: 16,781 Loc: Gaming the syste Last seen: 12 hours, 35 minutes |
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Who’s gonna take the medal for longest germination?!
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Convicted mycophile Registered: 07/23/18 Posts: 458 Last seen: 7 months, 16 days |
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Aum Mani Padme Hum Registered: 08/20/19 Posts: 1,742 Loc: Puerto Rico Last seen: 12 days, 11 hours |
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I want to thank everyone! I couldn't have done it without you.
For this Steppenwolf this is a wonderful community. So here is the conclusion for PESA spores from Filthy to agar, isolated to only T1 and got away with it! And even learned how to print! 40 Around 300g ea box...4 boxes ![]() ![]() ![]() ![]() ![]()
Edited by Inthepit (03/13/20 07:39 AM)
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Multispore Enthusiast Registered: 05/27/16 Posts: 3,951 Loc: ked in the trunk |
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ComebackKid's Grow Log
Species: Cubensis Var: PBR x Albino Species: Pan Cyan Var: Hawaii Species: Pan Cambo Var: Sandos DEC 18 2019 Waiting on Melmak swabs in the mail... hope they get here before the 1st ![]() JAN 1 2020 Haven't got Melmac spores in the mail yet. So I'm swabbing the gills of one of these PBR caps that I mixed with some albino spawn. Let's see what happens ![]() JAN 5 2020 I've got growth on one of my plates! Potato phone quality photos ![]() JAN 7 2020 Noticing some germination on the second plate now. Made my first transfer from the first plate. Only took one transfer. Kept the germ plates just in case something goes wrong but should be fine JAN 10 2020 Things are really getting out of control now. I've got cultures out the ying yang over here in the germination station. Put some Pan Cyan and Pan Cambo spores to agar today. Trying something new. Thanks to Mary Fairchild for the gift ![]() JAN 17 2020 ABX So after 1 transfer the cube myc is looking clean and uniform. Gunna drop this plate to three jars of grain whenever I find time. Pan Cambo Sandos Took one transfer from the whispiest looking growth. Holding onto the germ plate for now. Pan Cyan Hawaii All wispy growth.... Is this even what I'm looking for? Took one transfer and holding onto the germ plate to keep an eye on it. Grain Prep JAN 18 2020 Soaked 4.5 quarts of wheat kernels overnight ~24 hours JAN 19 2020 -Boiled water in 23 qt presto -Strained the soaked grain then added grain to boiling water -Boiled ~15 minutes -Left to steam dry in colander for ~ 1 hour -Loaded 12 quart jars 3/4 full (3 cups each) -PC 90 minutes at ~15 PSI -Let cool overnight JAN 20 2020 Inoculated 3 jars with agar wedges Then gave 'em a good shake JAN 25 2020 T1 Pan Cambo So now I'm just questioning everything at this point ![]() This Pan Cambo myc looked good to me on the germ plate.... but lookin fuzzy as hell on the T1 Took two transfers anyway. But I think it might be back to the drawing board for these Pan cultures. FEB 06 2020 Spawned half a tub with ABX Other half with a PE clone ABX 2.25 qts spawn 1/2 brick coir 1 qt verm 2.5 at water FEB 10 2020 Earlier this month I germinated some more Pan Cambo and Cyan spores. Took about 4 samples from each a couple days ago and today I took some more transfers from the cyan and cambo germ plates. The mycelium is looking a lot better this time around. I stopped going after the whispy growth and started looking for something that looked more like crons culture. I'll have lots to choose from soon! Pan cambo Only one spot looking good imo took a transfer Pan cyan Lots of places to choose from I took two samples from this plate FEB 18 2020 Been narrowing down my plates. I think I'm going to run with these. Pan cyan Hawaii Pan cambo Sandos I don't have much to pick from with the cambos. This was the best looking myc I could find. I'm just going to run this and see what happens FEB 24 2020 ABX 18 days after spawn Pinset is starting to fill out So far no traces of the albino genes in sight FEB 28 2020 ABX 22 days after spawn Edited by ComebackKid (02/28/20 05:30 AM)
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Stranger Registered: 10/11/18 Posts: 1,466 |
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I'm in for sure. Give me a reason to streak this purple mystic print I got.
Plates are 500 grams water. 8 grams LME 6.5 grams telephone brand agar agar. Streak a fairly fresh print with q-tips that I sterilize in a pint jar with a grain lid for 20 min at 15psi. I just do this at the same time I cook the agar if it works out that way. 1/1/20 8am: Spores just hit agar. Ill get a pic of the first colonies I grab. Update. 1/3/20 8am. Don't have time to transfer now but we got colonies! Purple Mysitc print was good BSM. 8:00am 1/7/20 Grabbed up some transfers. One didn't do shit. One is mold. But one... 8pm 1/8/20 Looking a little bacterial but in the spirit of competition I'm gonna throw it to grain and top fruit the jar to try and get an open fruit ASAP. I'm also gonna transfer it to some black tea agar to see if that cleans it up any. I've never done it before so I figure now is a good time to try. Then ill go back to MEA before starting a master jar. 1/9/20 Cooking up some grains for tommorow AM. Kinda nice to just use this little guy and not have to bust out the presto. 1/10/20 Dropped some wedges to jars. Fucking forgot to tighten the lids back down in the sab so... Probably fucked but that's ok if it wasn't for the race I wouldn't even have thought about grains yet I would have just made another few transfers. Only time will tell what else made it in the jars. The grains are pop corn that I boiled till tender (about 30 min simmer) then I added WBS that had been rinsed very well and cooked till some of the Millet burst then strained and cooled it till the grains were dry to the touch loaded in jars and cooked for 2 hours at 15psi. It's official I'm out of the first to open cap race. The two jars I made from the t1 plate didn't recover after the shake so I'm just gonna foucus on making a few more transfers. I don't like the look of this culture so I'm no longer in a rush to go to grain. Got some black tea today, I'm gonna make up some plates to see if that helps assuming its bacteria. I'm also going to make a pasty plate with BRF and transfer a wedge to see if I can outrun it there. I haven't done either but Im interested to try as it seems like a good skill to have. But I think I may have some mold in there as well cause one of my first transfers was straight mold so the tea and BRF my be pointless. Just in case I'm also gonna streak some PEUW spores and more purple mystic to see if I can get a clean culture on the fly and get some spawn for these pics. Not sure what's up. I suck at taking pics of cultures but I'm not feeling these rings. Take two. Well the culture is still looking weak at best but maybe it's not even myc. Going to throw it to grains one more time and if that's a no go that's a no go ill streak more spores and start over. Edited by Bph (02/08/20 01:29 PM)
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AstroMan Registered: 12/09/18 Posts: 2,144 Loc: Valleys Of Neptune Last seen: 1 day, 18 hours |
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1/1 Alright let’s grow some fucking mushrooms ![]() I decide on 4 cube varieties. Ape - Orissa India - AA+ - Mazatapec Each variety went to 5 plates and now we wait 1/7 Well I must have received some shitty syringes. Most of my plates are trash except for the ape plates, I’ll take transfers from them in the next day or two. I decided to start more spore plates sense most of them are trash. I put GT, LGT, Nepal, RW, AA+, Ecuador, and KSSS all to plates just a bit ago. All from prints this time, syringes don’t seem to like me. 1/9 Took my first Ape transfers from one of my spore plates today. I’ll probably take transfers from the others ape plates tomorrow. Edited by SFS96 (01/09/20 11:03 PM)
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Flight Attendant Registered: 09/22/19 Posts: 1,005 Loc: Psychonaut City, |
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~TeaforTwo 2020 grow log~![]() FeedTheWorld! ForTheWin!! ![]() Species: P.Cubensis Var: PESA + Texas Orange Cap + Purple Mystic 30&31Dec poured 60 plates, 20ea BRF, MEA, PDA ![]() 1Jan noc'd 3ea parent plates on BRF agar ![]() PESA and TOC from Tribe giveaway prints ![]() PM from syringe we began working with end of Oct which has so far produced 2 fruits neither of which dropped a single spore ![]() the parent spread ![]() (includes GT parent plate with a possible leucustic pin transfer to see what happens)
Edited by TeaforTwo (01/02/20 11:13 AM)
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swab
That's why I took T2's.




My dinner tonight came in one of the others: 






Jan 10th~ I did some transfers of Golden Halo, RustyWhyte, and Tailfeathers!




~TeaforTwo 2020 grow log~


