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OfflineA.k.aM
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26390502 - 12/19/19 11:08 AM (4 years, 1 month ago)

I think your lone vs cluster fruit question.

I’d def clone that big one. It looks cool so if it happens to be a mutation it might show up again and if not it’s obviously got good genes for growth.

I haven’t done pasty plates but that looks like enough water to warrant a draining.


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: A.k.a]
    #26390711 - 12/19/19 01:14 PM (4 years, 1 month ago)

Ok gotcha just wasn't sure if be was referencing me or someone else...


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: SpunkyMonkey88] * 1
    #26390817 - 12/19/19 02:33 PM (4 years, 1 month ago)

The same question you had above "can you still get a nice flush from cloning a fat "lone ranger"".

I'm gonna take a few samples tomorrow morning, and I'll take some separately from the cluster ones and the big one and make an experiment myself. I'll keep a log with progress on this thread. Lets see in practice. (Its my first time attempting this and first go at agar, sab, cloning etc.)


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OfflinePhycus
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390839 - 12/19/19 02:55 PM (4 years, 1 month ago)

curious myself


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26390850 - 12/19/19 03:02 PM (4 years, 1 month ago)

Quote:

Shr00merN00ber said:
The same question you had above "can you still get a nice flush from cloning a fat "lone ranger"".

I'm gonna take a few samples tomorrow morning, and I'll take some separately from the cluster ones and the big one and make an experiment myself. I'll keep a log with progress on this thread. Lets see in practice. (Its my first time attempting this and first go at agar, sab, cloning etc.)




Ok gotcha...hope your prepared to have a shit ton of plates and be ready to make weekly transfers... basically those plates keep adding up, quickly too.

Dont be afraid to dump plates. I've only been playing around with 2 strains (well three but 1 wasn't ever put to grain) and I only made it to T3s and I've probably dumped 30 plates and am sitting on 15 or so... and that's actually pretty good.

So if you plan on making a bunch of clones I hope you got a ton of plates on hand cus ull need em.

Definitely let us know about the clones though...i never see any threads outlining grows that were from clone cultures, or I'm not paying enough attention...


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OfflineA.k.aM
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26390942 - 12/19/19 04:05 PM (4 years, 1 month ago)

I just put a clone plate from a big solitary fruit to grain two days ago. The myc jumped off real quick so off to a good start. My first grow from agar and first clone also.


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: A.k.a]
    #26391442 - 12/19/19 08:17 PM (4 years, 1 month ago)


Quote:

A.k.a said:
I just put a clone plate from a big solitary fruit to grain two days ago. The myc jumped off real quick so off to a good start. My first grow from agar and first clone also.







Edited by SpunkyMonkey88 (12/19/19 08:18 PM)


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26391797 - 12/20/19 02:10 AM (4 years, 1 month ago)

So I gave it a shot this morning and it was generally harder than what I thought. Working in the sab can be annoying and I was sweating quite a lot towards the end, which made my mind go blank and I'm not 100% sure of which transfers went to which plate in the end!! I'm 80% sure... Dammit
Here are some photos







This guy should be safe to eat right?







Argh I'm so pissed at myself for not keeping 100% track of which specimens were going where.. Also one of my agar plates had the consistency of a slushy when I opened it in the SAB, and another just fell out on the base when I tried to tip some water out. Only 4 transfers in total. Will update in a few days.!


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OfflineA.k.aM
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26392182 - 12/20/19 09:46 AM (4 years, 1 month ago)

Lol good luck. I see you had the same patience for melting through the plastic to make arm holes as I did.

I hope that cap is genetic.


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: A.k.a]
    #26392185 - 12/20/19 09:50 AM (4 years, 1 month ago)

Haha, I wish its not genetic! Its safe to eat though right? Now thats its dried it looks ok but when I look at that photo.. hmmm :confused:


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OfflineA.k.aM
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26392200 - 12/20/19 10:00 AM (4 years, 1 month ago)

I meant the split cap I like that one for some reason. I usually get a couple with that weird ball on top with golden teachers.  all of them should be fine to eat.


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26392202 - 12/20/19 10:01 AM (4 years, 1 month ago)

It's a mushroom nipple


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26392218 - 12/20/19 10:09 AM (4 years, 1 month ago)

:ohshi:


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26398870 - 12/24/19 09:20 AM (4 years, 1 month ago)

Hey guys update. Here are the plates 4 days later. Out of the 4 I had, I knocked one on the floor and the tissue sample went on the side and hasn't really recovered. Here are the three that are going:

Plate 1 - (There are actually 2 tissue samples in this plate, pretty close to each other, the sample split when I was trying to move it to the center...!) 





Plate 2



Plate 3




Plates 1 & 2 should be the clones from the big boy, plate 3 from the cluster. Plate 2 looks more white irl, but not as much as plate 1.

So my question, do I wait a few more days for the myc to grow out or shall I transfer asap?

I'm just waiting for my pc to cool and will attempt my first go at pouring petris. Wish me luck!


Edited by Shr00merN00ber (12/24/19 09:22 AM)


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26398900 - 12/24/19 09:32 AM (4 years, 1 month ago)

Quote:

Shr00merN00ber said:
Hey guys update. Here are the plates 4 days later. Out of the 4 I had, I knocked one on the floor and the tissue sample went on the side and hasn't really recovered. Here are the three that are going:

Plate 1 - (There are actually 2 tissue samples in this plate, pretty close to each other, the sample split when I was trying to move it to the center...!) 





Plate 2



Plate 3




Plates 1 & 2 should be the clones from the big boy, plate 3 from the cluster. Plate 2 looks more white irl, but not as much as plate 1.

So my question, do I wait a few more days for the myc to grow out or shall I transfer asap?

I'm just waiting for my pc to cool and will attempt my first go at pouring petris. Wish me luck!




Personally I would wait a day or so...

My theory is after its established s bit more the outer edges will be that much further away from any contaminated tissue...
Usually something like this for me:


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26398905 - 12/24/19 09:38 AM (4 years, 1 month ago)

Thanks so much Spunky for your help in all of this. I was wondering about the size of sample, do you take about 1/2 cm? Lets take your pic as an example, where would you take from and which sections would you avoid?


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26398917 - 12/24/19 09:45 AM (4 years, 1 month ago)

Quote:

Shr00merN00ber said:
Thanks so much Spunky for your help in all of this. I was wondering about the size of sample, do you take about 1/2 cm? Lets take your pic as an example, where would you take from and which sections would you avoid?




Well I had a TC tell me that 5 o'clock looked good on that plate I just posted. But I'm less than 50 plates deep into this hobby so i cant tell you what i would take from yours...another tc said I could basically take from anywhere on that plate as it all looked pretty good.

I think alot of the time we over think sector selection, I think the main thing is avoiding contaminated parts.

I also have been practicing taking very small pieces like the size of a grain of rice. I even just scraped a bit off of the edge with a scaple and stabbed the middle of the new plate and that worked well...

Thw smaller the better IMO...probably the only time you'll hear that in life lol



Edited by SpunkyMonkey88 (12/24/19 09:50 AM)


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26398921 - 12/24/19 09:49 AM (4 years, 1 month ago)

Quote:

SpunkyMonkey88 said:
Quote:

Shr00merN00ber said:
Thanks so much Spunky for your help in all of this. I was wondering about the size of sample, do you take about 1/2 cm? Lets take your pic as an example, where would you take from and which sections would you avoid?




Well I had a TC tell me that 5 o'clock loomed good on that plate I just posted. But I'm less than 50 plates deep into this hobby so i cant tell you what i would take from yours...another tc said I could basically take from anywhere on that plate as it all looked pretty good.

I think alot of the time we over think sector selection, I think the main thing is avoiding contaminated parts.

I also have been practicing taking very small pieces like the size of a grain of rice. I even just scraped a but off of the edge with a scaple and stabbed the middle of the new plate and that worked well...

Thw smaller the better IMO...probably the only time you'll hear that in life lol





Haha that last part made me laugh LOL. I'll post some pics after pour and some after transfer in a few days. Merry xmas everyone and thanks again for your help!


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InvisibleSpunkyMonkey88
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Re: First time will attempt cloning [Re: Shr00merN00ber]
    #26398938 - 12/24/19 09:58 AM (4 years, 1 month ago)

No problem man, merry Christmas!


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OfflineShr00merN00ber
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Re: First time will attempt cloning [Re: SpunkyMonkey88]
    #26399015 - 12/24/19 11:41 AM (4 years, 1 month ago)

Just got done pouring and wrapping with parafilm. Man that parafilm can get annoying with cutting and stretching etc. Here is how it looks. I dont know why I got alot of condensation on some plates (not all), I had the bottle in a water bath at 47C for a while before. Some dishes have these bits in it, all good or  ones with bits looking trash? I seem to find the pieces settled on the bottom.









Cheers!


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