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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 549
Loc: UK
Last seen: 16 days, 15 hours
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Liquid Culture from spores.
#26386981 - 12/17/19 12:30 PM (4 years, 1 month ago) |
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Has anyone had success making liquid culture from spores?
I’ve tried once but failed.
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PopaCap
Convicted mycophile


Registered: 07/23/18
Posts: 458
Last seen: 7 months, 17 days
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Re: Liquid Culture from spores. [Re: Zombie-ant]
#26386999 - 12/17/19 12:44 PM (4 years, 1 month ago) |
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It’s just not a good idea. Spores are always gonna be exposed to open air, unless collected from a plate fruit. Not worth the risk when you have a much better chance of having clean LC started from healthy plate growth.
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trubblesome
Stranger


Registered: 11/09/19
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Re: Liquid Culture from spores. [Re: PopaCap]
#26387005 - 12/17/19 12:46 PM (4 years, 1 month ago) |
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I did it, and I got one flush in a race against trich. not worth it. if only i'd known that then.
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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 549
Loc: UK
Last seen: 16 days, 15 hours
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Re: Liquid Culture from spores. [Re: PopaCap]
#26387038 - 12/17/19 01:01 PM (4 years, 1 month ago) |
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Quote:
PopaCap said: It’s just not a good idea. Spores are always gonna be exposed to open air, unless collected from a plate fruit. Not worth the risk when you have a much better chance of having clean LC started from healthy plate growth.
Is it worth doing more than one agar plate?
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Liquid Culture from spores. [Re: Zombie-ant]
#26387043 - 12/17/19 01:04 PM (4 years, 1 month ago) |
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One agar plate is never enough. And once you start, you will end up buying a case of 500. Speaking of which I need a new case again 🙄
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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PopaCap
Convicted mycophile


Registered: 07/23/18
Posts: 458
Last seen: 7 months, 17 days
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Re: Liquid Culture from spores. [Re: Zombie-ant]
#26387045 - 12/17/19 01:05 PM (4 years, 1 month ago) |
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Why do you only have 1 agar plate?
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PopaCap
Convicted mycophile


Registered: 07/23/18
Posts: 458
Last seen: 7 months, 17 days
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Re: Liquid Culture from spores. [Re: Zombie-ant]
#26387061 - 12/17/19 01:11 PM (4 years, 1 month ago) |
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Quote:
Zombie-ant said:
Is it worth doing more than one agar plate?
That’s up to you. I sure as hell wouldn’t do it. I don’t enjoy investing my time and energy into a grow that has a fairly good chance of turning to shit.
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The Mycologist
Explorer

Registered: 05/06/16
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Last seen: 29 days, 8 hours
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Re: Liquid Culture from spores. [Re: PopaCap]
#26387073 - 12/17/19 01:20 PM (4 years, 1 month ago) |
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Do 15 agar plates
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Zombie-ant
Mushroom Ninja in training



Registered: 09/02/19
Posts: 549
Loc: UK
Last seen: 16 days, 15 hours
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Re: Liquid Culture from spores. [Re: PopaCap]
#26387074 - 12/17/19 01:20 PM (4 years, 1 month ago) |
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I have two glass ones, i want to reduce my plastic usage
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A.k.a
Stranger



Registered: 10/27/19
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Re: Liquid Culture from spores. [Re: Zombie-ant]
#26387085 - 12/17/19 01:26 PM (4 years, 1 month ago) |
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I’ve got 4 ms lcs, one from each syringe I started with.
I just tested one of them and it worked surprisingly well, but it was also the one I think had the best chance I’m not very confident in the other ones.
I think getting pins on agar takes a decent chunk of time/luck but I’m not sure.
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LAGM2020     
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Liquid Culture from spores. [Re: Zombie-ant]
#26387086 - 12/17/19 01:27 PM (4 years, 1 month ago) |
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Quote:
Zombie-ant said: I have two glass ones, i want to reduce my plastic usage
Well I guess u could keep transferring between plates but you're gonna be doing a shit load of pressure cooking and you'll be limited to one transfer per new plate AND after you make that transfer you'll have to dump your parent plate so you have another clean one...
If you want to cut down on plastics, do it by cutting them out of another aspect of your life, not mush cult... or buy more glass petris...
It is just isn't practical at all to do agar with two plates..
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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I did this once and every single brf jar I knocked up was crazy bacterial with a bunch of gunk at the bottom or yellow metabolites around the glass between the mycelium.
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YogiBear



Registered: 08/24/19
Posts: 845
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PopaCap
Convicted mycophile


Registered: 07/23/18
Posts: 458
Last seen: 7 months, 17 days
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If you want to advance in this hobby with any kind of repeatable success, you’re gonna have to either buy more glass plates, or give the planet the middle finger and buy a bunch of plastic ones. You could always cut down the plastic usage by purchasing y or x petris.
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The Mycologist
Explorer

Registered: 05/06/16
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Last seen: 29 days, 8 hours
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Re: Liquid Culture from spores. [Re: Zombie-ant]
#26388193 - 12/18/19 06:33 AM (4 years, 1 month ago) |
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Yea I love my glass plates. Get yourself some more. Its so worth it. Agar is the secret to unlimited inoculate.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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If you use glass you are going to be constantly sterilizing. In the process, using propane or electricity generated by coal burning or splitting atoms, not to mention emptying your pocket. Pick your battles and buy plastic Petris that are manufactured with likely a small fraction of environmental impact you picture.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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Psilotyl
נָזִיר


Registered: 08/30/19
Posts: 469
Loc: עולם
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Re: Liquid Culture from spores. [Re: Smartattack]
#26388458 - 12/18/19 10:17 AM (4 years, 1 month ago) |
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I knocked up 12 pint sized honey LC's, 6 different strains, about 10 days ago. Pray for me. Growth looking good so far, for whatever that's worth.
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שלום וְאור | PEACE & LIGHT
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 29 days, 8 hours
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Re: Liquid Culture from spores. [Re: Smartattack]
#26388532 - 12/18/19 11:02 AM (4 years, 1 month ago) |
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The PC is running either way.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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