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OfflineZombie-ant
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Liquid Culture from spores.
    #26386981 - 12/17/19 12:30 PM (4 years, 1 month ago)

Has anyone had success making liquid culture from spores?

I’ve tried once but failed.


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OfflinePopaCap
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Re: Liquid Culture from spores. [Re: Zombie-ant]
    #26386999 - 12/17/19 12:44 PM (4 years, 1 month ago)

It’s just not a good idea. Spores are always gonna be exposed to open air, unless collected from a plate fruit. Not worth the risk when you have a much better chance of having clean LC started from healthy plate growth.


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Offlinetrubblesome
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Re: Liquid Culture from spores. [Re: PopaCap]
    #26387005 - 12/17/19 12:46 PM (4 years, 1 month ago)

I did it, and I got one flush in a race against trich. not worth it. if only i'd known that then.


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OfflineZombie-ant
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Re: Liquid Culture from spores. [Re: PopaCap]
    #26387038 - 12/17/19 01:01 PM (4 years, 1 month ago)

Quote:

PopaCap said:
It’s just not a good idea. Spores are always gonna be exposed to open air, unless collected from a plate fruit. Not worth the risk when you have a much better chance of having clean LC started from healthy plate growth.




Is it worth doing more than one agar plate?


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InvisibleSmartattack
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Re: Liquid Culture from spores. [Re: Zombie-ant]
    #26387043 - 12/17/19 01:04 PM (4 years, 1 month ago)

One agar plate is never enough. And once you start, you will end up buying a case of 500. Speaking of which I need a new case again 🙄


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* Smarts videos :teacher:
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OfflinePopaCap
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Re: Liquid Culture from spores. [Re: Zombie-ant]
    #26387045 - 12/17/19 01:05 PM (4 years, 1 month ago)

Why do you only have 1 agar plate?


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OfflinePopaCap
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Re: Liquid Culture from spores. [Re: Zombie-ant]
    #26387061 - 12/17/19 01:11 PM (4 years, 1 month ago)

Quote:

Zombie-ant said:

Is it worth doing more than one agar plate?




That’s up to you. I sure as hell wouldn’t do it. I don’t enjoy investing my time and energy into a grow that has a fairly good chance of turning to shit.


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OfflineThe Mycologist
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Re: Liquid Culture from spores. [Re: PopaCap]
    #26387073 - 12/17/19 01:20 PM (4 years, 1 month ago)

Do 15 agar plates


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineZombie-ant
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Re: Liquid Culture from spores. [Re: PopaCap]
    #26387074 - 12/17/19 01:20 PM (4 years, 1 month ago)

I have two glass ones, i want to reduce my plastic usage


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OfflineA.k.aM
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Re: Liquid Culture from spores. [Re: Zombie-ant]
    #26387085 - 12/17/19 01:26 PM (4 years, 1 month ago)

I’ve got 4 ms lcs, one from each syringe I started with.

I just tested one of them and it worked surprisingly well, but it was also the one I think had the best chance I’m not very confident in the other ones.


I think getting pins on agar takes a decent chunk of time/luck but I’m not sure.


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InvisibleSpunkyMonkey88
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Re: Liquid Culture from spores. [Re: Zombie-ant]
    #26387086 - 12/17/19 01:27 PM (4 years, 1 month ago)

Quote:

Zombie-ant said:
I have two glass ones, i want to reduce my plastic usage




Well I guess u could keep transferring between plates but you're gonna be doing a shit load of pressure cooking and you'll be limited to one transfer per new plate AND after you make that transfer you'll have to dump your parent plate so you have another clean one...

If you want to cut down on plastics, do it by cutting them out of another aspect of your life, not mush cult... or buy more glass petris...

It is just isn't practical at all to do agar with two plates..


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InvisibleSpunkyMonkey88
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Re: Liquid Culture from spores. [Re: SpunkyMonkey88]
    #26387102 - 12/17/19 01:34 PM (4 years, 1 month ago)

I did this once and every single brf jar I knocked up was crazy bacterial with a bunch of gunk at the bottom or yellow metabolites around the glass between the mycelium.



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InvisibleYogiBear
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Re: Liquid Culture from spores. [Re: SpunkyMonkey88]
    #26387118 - 12/17/19 01:46 PM (4 years, 1 month ago)



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OfflinePopaCap
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Re: Liquid Culture from spores. [Re: SpunkyMonkey88]
    #26387121 - 12/17/19 01:46 PM (4 years, 1 month ago)

If you want to advance in this hobby with any kind of repeatable success, you’re gonna have to either buy more glass plates, or give the planet the middle finger and buy a bunch of plastic ones. You could always cut down the plastic usage by purchasing y or x petris.


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OfflineThe Mycologist
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Re: Liquid Culture from spores. [Re: Zombie-ant]
    #26388193 - 12/18/19 06:33 AM (4 years, 1 month ago)

Yea I love my glass plates. Get yourself some more. Its so worth it. Agar is the secret to unlimited inoculate.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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InvisibleSmartattack
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Re: Liquid Culture from spores. [Re: The Mycologist]
    #26388387 - 12/18/19 09:28 AM (4 years, 1 month ago)

If you use glass you are going to be constantly sterilizing. In the process, using propane or electricity generated by coal burning or splitting atoms, not to mention emptying your pocket. Pick your battles and buy plastic Petris that are manufactured with likely a small fraction of environmental impact you picture.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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InvisiblePsilotyl
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Re: Liquid Culture from spores. [Re: Smartattack]
    #26388458 - 12/18/19 10:17 AM (4 years, 1 month ago)

I knocked up 12 pint sized honey LC's, 6 different strains, about 10 days ago. Pray for me. Growth looking good so far, for whatever that's worth.


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‎שלום וְאור | PEACE & LIGHT


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OfflineThe Mycologist
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Re: Liquid Culture from spores. [Re: Smartattack]
    #26388532 - 12/18/19 11:02 AM (4 years, 1 month ago)

The PC is running either way.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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