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Offlineinsutama
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Could the agar im using be causing the growth to look like this (pics)
    #26385425 - 12/16/19 04:01 PM (4 years, 1 month ago)

So i have done probally around 40 plates of different strains and up to T5 on certain ones and i just cant seem to get the growth im looking for Could it be my agar thats expired or what would cause all of my plates to look like this its really faint and hard to see any growth without been under a light i included a pic of a pasty plate i made 4 months ago and inoculated it the same time as the rest of these plates its almost like the old pasty plate has thicker better growth than these fresh petris could you all let me know if this is contamination or just a weird not so thick looking growth. The agar receipe is 7grams LME and 10 grams agar with 500ml water
Bottom of plate

Top of same plates above


and the old pasty plate


Edited by insutama (12/16/19 04:04 PM)


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OfflineBlueTryptoYoshi
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26385632 - 12/16/19 06:08 PM (4 years, 1 month ago)

I'm finding that cubes like mea agar in my experience so far.


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InvisiblemushboyMDiscord
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: BlueTryptoYoshi] * 1
    #26385646 - 12/16/19 06:12 PM (4 years, 1 month ago)

Those dishes look like mold to me:shrug:

I stopped using lme in my agar tho because I always had these results.


Maybe I wasnt using enough?


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InvisibleSmartattack
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: mushboy]
    #26385786 - 12/16/19 07:11 PM (4 years, 1 month ago)

I agree with Mushboy it looks like mold. However agar recipes can make shit weird. My myc looked like that when my nute. % was too high.


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: Smartattack]
    #26385807 - 12/16/19 07:17 PM (4 years, 1 month ago)

its weird though because all my plates are looking like this but there is only growth where i put spores and transfers i just dont know how to get away from this i cant seem to get any good growth should i leave them and see if they change color ?


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: mushboy]
    #26385814 - 12/16/19 07:18 PM (4 years, 1 month ago)

Quote:

mushboy said:
Those dishes look like mold to me:shrug:

I stopped using lme in my agar tho because I always had these results.


Maybe I wasnt using enough?



what do you use now ?


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Offlineunibrowscowl
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26385885 - 12/16/19 07:59 PM (4 years, 1 month ago)

I use a 1:1 ratio with my  MEA agar. I do 10 grams of both in 500 ml of water and almost all my myc growth has been consistently rhizomorphic.  Smartattack said he got similar results with too much nutrients.  Possibly too little nutrients can cause fuzzy growth as well?

This thread has also been very useful to me in my agar endeavors:
https://www.shroomery.org/forums/showflat.php/Number/18676888


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OfflineMycoactive
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26385917 - 12/16/19 08:12 PM (4 years, 1 month ago)

I don't think that's contamination. I've had plates that look very similar that were made with old agar. I have no idea why the plates can still set well and appear totally normal yet promote different types of growth, but I've definitely seen it happen before. There must be some subtle differences in the agar's molecular structure.  :shrug:


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: Mycoactive]
    #26385924 - 12/16/19 08:14 PM (4 years, 1 month ago)

intresting maybe ill try different agar when i make my next plates


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InvisiblemushboyMDiscord
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26385927 - 12/16/19 08:16 PM (4 years, 1 month ago)

Quote:

insutama said:
Quote:

mushboy said:
Those dishes look like mold to me:shrug:

I stopped using lme in my agar tho because I always had these results.


Maybe I wasnt using enough?



what do you use now ?




brf. 6g 500ml water. not clear but i think its easier to spot contams on opaque surfaces:shrug:




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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: mushboy]
    #26386021 - 12/16/19 08:52 PM (4 years, 1 month ago)

i guess if its my agar thats gone bad it wont matter what nutrients i use i just ordered some different agar off amazon ill try making plates with that once it gets here. I think the telephone brand agar i bought from a local Asian market store was very old and that may be why all my plates look like this even my clones looked like this and i put them to jars and so far the jars are doing great no signs of contams


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26386054 - 12/16/19 09:16 PM (4 years, 1 month ago)

If agar goes bad would that mean its just less effective so if i added more it would make up for the older agar ? because my agar seemed really sticky when i put wedges in jars so if i used say 20 grams instead of 10 grams could that possibly make firmer agar and with the agar been firmer maybe i would see different growth ?


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Offlineherbnmyco
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: unibrowscowl]
    #26386096 - 12/16/19 09:46 PM (4 years, 1 month ago)

Im with mushboy I dont like lme agar I got crappy results. My favorite agar is brown rice flower. 2% agar and brf


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: herbnmyco]
    #26392880 - 12/20/19 06:06 PM (4 years, 1 month ago)

I just made some new agar using 6 grams BRF and 10 grams agar with 500ml water does this sound like the right ratios ?


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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26392904 - 12/20/19 06:21 PM (4 years, 1 month ago)

:awethumb:


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OfflinecronicrFacebook
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26392905 - 12/20/19 06:21 PM (4 years, 1 month ago)

:super:


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: cronicr]
    #26395712 - 12/22/19 02:26 PM (4 years, 1 month ago)

so far not looking good i did a transfer to some of the new plates and it looks like translucent growth can only really see anything if held under a light so im thinking the BRF didnt provide enough nutrition or the plates i transferred from are bacterial anyway here are some pics

Plate made with new agar and LME 10grams of each 500ml water on dec 20th


plate made with 10 grams new agar and 6 grams BRF and 500ml water



sorry for the poor pic quality it was hard to get the angle right to show this

I also started some plates with fresh spores that have no signs of growth yet but encase these plates are taken from a bad plate i can see how fresh spores do on this new agar


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26398185 - 12/23/19 07:47 PM (4 years, 1 month ago)

Anyone know why my BRF agar is growing only translucent like mycelium ? does this mean lack of nutrients ?


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InvisiblemushboyMDiscord
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26398200 - 12/23/19 07:57 PM (4 years, 1 month ago)

did you filter the brf?


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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26398234 - 12/23/19 08:14 PM (4 years, 1 month ago)

Quote:

insutama said:
Anyone know why my BRF agar is growing only translucent like mycelium ? does this mean lack of nutrients ?



Mine grows fine:shrug:


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Re: Could the agar im using be causing the growth to look like this (pics) [Re: insutama]
    #26398244 - 12/23/19 08:18 PM (4 years, 1 month ago)



unfiltered. 6g brf 9g telefone 500ml tap water


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Offlineinsutama
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Re: Could the agar im using be causing the growth to look like this (pics) [Re: mushboy]
    #26398707 - 12/24/19 06:43 AM (4 years, 1 month ago)

Quote:

mushboy said:
did you filter the brf?




nope

ill wait and see how the plates look i put spores on maybe that translucent growth is bacterial growth


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