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OfflineHighHarles
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Registered: 07/07/18
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B+ Agar Plate
    #26385246 - 12/16/19 02:17 PM (4 years, 1 month ago)


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Its thick like a donut, for sure has myc underneath
I used an older Spore syringe that was gifted to me. I tried innoculating 20 jars and 5 plates. This one plate worked and 7 jars had some growth. One of the seven seems to be successful.

Is this just a super dense strain variety on the agar plate? Why would my success rate be so weird? I seem to have a really hard time with Spore syringes.


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OfflineMycoactive
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Re: B+ Agar Plate [Re: HighHarles]
    #26386658 - 12/17/19 08:49 AM (4 years, 1 month ago)

When you say your other plates and jars weren't successful, do you mean they were contaminated and/or did they show no signs of growth? Are you shaking the syringes before using to distribute the spores evenly? Did you use that one syringe to inoculate all 20 jars and 5 plates? If there weren't many spores in the syringe, you're spreading them pretty thin.


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OfflineBLINKfan420
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Re: B+ Agar Plate [Re: Mycoactive]
    #26386699 - 12/17/19 09:21 AM (4 years, 1 month ago)

Quote:

Mycoactive said:If there weren't many spores in the syringe, you're spreading them pretty thin.




If there are spores in the syringe there are probably a few thousand at the minimum. Maybe 50,000? Idk but a fucking lot regardless.

The thick growth on the agar is probably due to your recipe.  Try less nutrients for starters but same water and agar ratio.

Syringe to jars can be tough.  You're not giving the spores and very good fighting chance. That's why its recommended to go syringe>agar>jars unless you're doing BRF jars.

BLiNK


--------------------



All my pictures are drawn from imagination.  I don't even know what a mushroom is.


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OfflineHighHarles
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Re: B+ Agar Plate [Re: BLINKfan420]
    #26386855 - 12/17/19 10:54 AM (4 years, 1 month ago)


I'm sure a few of these wont colonize
I usually go agar to grain to start with but if I could get Spore variety to do a large grain to grain transfer for a couple tubs thatd be worth a shot
Golden teachers agar to grain are above the B+ Jars

I'm not that new to this, so yes, I shook the syringe. I just never seem to have good luck with Spore syringes, ever.


Edited by HighHarles (12/17/19 09:43 PM)


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OfflineHighHarles
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Re: B+ Agar Plate [Re: HighHarles]
    #26386859 - 12/17/19 10:58 AM (4 years, 1 month ago)

I have several agar recipes saved from forums on here.
Malt agar
20g agar-agar, 15g light malt extract, 1L</= h2o)

Brf agar
500 mls of water
10 to 12 grams of agar
10 grams of brf.

Honey potatoe flakes agar
500 mls water
10 grams agar
7 grams of potatoes flakes
A teaspoon of honey.

Millet flour agar
500 mls water
12 grams agar
12 grams millet Flour.

Rye flour agar.
500 mls water
10 grams agar
9 grams rye flour
1 gram nutritional yeast

Dog food agar
500 mls water
12 grams agar
10 grams ground dried dog food
1 gram nutritional yeast

Starving agar
500 mls water
12 grams agar
Note: this is really only used for cloning or super dirty transfers

I've been using the first one since I started with no issues. I used to have a condensation problem, but I figured out I was pouring them thick af. Still kinda do but not as bad, I find it easy to make it a more level surface.
Is there one more suitable than the rest?


Edited by HighHarles (12/17/19 10:59 AM)


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OfflineHighHarles
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Re: B+ Agar Plate [Re: HighHarles]
    #26387852 - 12/17/19 09:31 PM (4 years, 1 month ago)

These would be the duds
11-28

Top shelf are all b+


Edited by HighHarles (12/17/19 10:45 PM)


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