|
HighHarles
Stranger


Registered: 07/07/18
Posts: 354
Last seen: 1 year, 2 months
|
B+ Agar Plate
#26385246 - 12/16/19 02:17 PM (4 years, 1 month ago) |
|
|
 Bottom
 Top Its thick like a donut, for sure has myc underneath I used an older Spore syringe that was gifted to me. I tried innoculating 20 jars and 5 plates. This one plate worked and 7 jars had some growth. One of the seven seems to be successful.
Is this just a super dense strain variety on the agar plate? Why would my success rate be so weird? I seem to have a really hard time with Spore syringes.
|
Mycoactive
Scientist


Registered: 11/20/19
Posts: 185
Last seen: 27 days, 43 minutes
|
|
When you say your other plates and jars weren't successful, do you mean they were contaminated and/or did they show no signs of growth? Are you shaking the syringes before using to distribute the spores evenly? Did you use that one syringe to inoculate all 20 jars and 5 plates? If there weren't many spores in the syringe, you're spreading them pretty thin.
|
BLINKfan420
Jedi



Registered: 07/06/12
Posts: 1,078
Last seen: 5 months, 16 hours
|
|
Quote:
Mycoactive said:If there weren't many spores in the syringe, you're spreading them pretty thin.
If there are spores in the syringe there are probably a few thousand at the minimum. Maybe 50,000? Idk but a fucking lot regardless.
The thick growth on the agar is probably due to your recipe. Try less nutrients for starters but same water and agar ratio.
Syringe to jars can be tough. You're not giving the spores and very good fighting chance. That's why its recommended to go syringe>agar>jars unless you're doing BRF jars.
BLiNK
--------------------
  All my pictures are drawn from imagination. I don't even know what a mushroom is.
|
HighHarles
Stranger


Registered: 07/07/18
Posts: 354
Last seen: 1 year, 2 months
|
|
 I'm sure a few of these wont colonize I usually go agar to grain to start with but if I could get Spore variety to do a large grain to grain transfer for a couple tubs thatd be worth a shot Golden teachers agar to grain are above the B+ Jars
 I'm not that new to this, so yes, I shook the syringe. I just never seem to have good luck with Spore syringes, ever.
Edited by HighHarles (12/17/19 09:43 PM)
|
HighHarles
Stranger


Registered: 07/07/18
Posts: 354
Last seen: 1 year, 2 months
|
|
I have several agar recipes saved from forums on here. Malt agar 20g agar-agar, 15g light malt extract, 1L</= h2o)
Brf agar 500 mls of water 10 to 12 grams of agar 10 grams of brf.
Honey potatoe flakes agar 500 mls water 10 grams agar 7 grams of potatoes flakes A teaspoon of honey.
Millet flour agar 500 mls water 12 grams agar 12 grams millet Flour.
Rye flour agar. 500 mls water 10 grams agar 9 grams rye flour 1 gram nutritional yeast
Dog food agar 500 mls water 12 grams agar 10 grams ground dried dog food 1 gram nutritional yeast
Starving agar 500 mls water 12 grams agar Note: this is really only used for cloning or super dirty transfers
I've been using the first one since I started with no issues. I used to have a condensation problem, but I figured out I was pouring them thick af. Still kinda do but not as bad, I find it easy to make it a more level surface. Is there one more suitable than the rest?
Edited by HighHarles (12/17/19 10:59 AM)
|
HighHarles
Stranger


Registered: 07/07/18
Posts: 354
Last seen: 1 year, 2 months
|
|
These would be the duds 11-28
 Top shelf are all b+
Edited by HighHarles (12/17/19 10:45 PM)
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,580 topic views. 29 members, 292 guests and 106 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|