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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 6 months, 5 days
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Sporulation of cold fruiting species
#26377641 - 12/12/19 04:29 PM (4 years, 2 months ago) |
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Hey everyone 
I tried sporeprinting Psilocybe cyanescens and got very little.. now I collected brick tops and late oyster and see nothing so far, indoors.
So do cold fruiting species stop sporulating quickly when brought to room temperature? And they should print okay outside?
The Ps. cyan was very weird by the way, even outdoors fruits seemed hard-pressed and reserved to produce spores and gills would often stay blank / tan colored even when curled up heavily.
Please someone with some knowledge on this shine a light on the matter, cause I am going professional and have started a serious myco bank of my own.. i like to give back too, but this puzzles and impedes me.
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karode13
Tāne Mahuta




Registered: 05/19/05
Posts: 15,290
Loc: LV-426
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Re: Sporulation of cold fruiting species [Re: Solipsis]
#26377670 - 12/12/19 04:53 PM (4 years, 2 months ago) |
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I generally don't have an issue with printing any of those species. Cyans could be sterile, have hyaline spores or perhaps it dropped most of its spores before you got to it..
Hypholoma produce a lot of spores generally.
Leave the prints to sit for 12 hours at least.
Keep trying.
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Anglerfish
hearing things



Registered: 09/08/10
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Loc: Norvegr
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Re: Sporulation of cold fruiting species [Re: Solipsis]
#26377689 - 12/12/19 05:06 PM (4 years, 2 months ago) |
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Did you remember to cover them with a cup or glass upside down?
I've also read you could leave them printing in the fridge for better results, but I never tried so can't tell if it works. At least it's helpful to keep them humid to drop the spores properly.
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★★★★★
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 6 months, 5 days
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Re: Sporulation of cold fruiting species [Re: Anglerfish]
#26381060 - 12/14/19 10:20 AM (4 years, 2 months ago) |
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Thanks guys!
Yes I generally remember to cover for both humidity/desiccation and also cleanliness... but surprisingly a small young Brick Top that i accidentally left on my workbench, mostly dried out and was the first to leave spores on the workbench haha. Fortunately the other brick tops i was actually printing have now sporedropped too, getting started slowly, a day or more later.
Late oyster nada..
Hyaline spores wouldn't that still give a certain optical effect on aluminum foil? Some sort of sheen or reflection difference? The Ps cyan i tried many attempts to print in many different ways. At the beginning of this seasons flush i got light but acceptable prints the regular way but after that nothing seemed to work.
Hyaline spores of tiered tooth fungus i was unable to see on aluminum foil pieces were wrapped in, so no idea if any significant number were there. streaked some to agar anyway in the hope there were.
An experienced friend said yes when i asked if mushrooms sporulate in different conditions that vary between species. Different temperature perhaps, idk what kind of cues some wait for.
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TheDuder
Mushroom Hunter



Registered: 11/07/16
Posts: 2,544
Loc: PNW
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Re: Sporulation of cold fruiting species [Re: Solipsis]
#26381211 - 12/14/19 12:05 PM (4 years, 2 months ago) |
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If the caps are on the drier side i usually put a drop of water on the cap while printing to rehydrate them a bit
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|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|
|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|
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Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 6 months, 5 days
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Re: Sporulation of cold fruiting species [Re: TheDuder]
#26383057 - 12/15/19 10:17 AM (4 years, 2 months ago) |
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Thanks, i do keep an eye out for drying too much - but in various cases here if anything it seemed like it might have helped a little.
I am really open to any insights and experiences regarding what can complicate getting spores. And anything on top of the basics of putting a cap or fruit on a surface (,covering) and being patient.
Like temperature for example. You figure there are do's and don'ts kinda like cold or heat shocks? Like maybe some species hold on to their spores more until its the optimal time. In that case I guess you might say just put them outside as the temps correspond to what they were in the middle of in the wild.
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Doc9151
Mycologist


Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
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Re: Sporulation of cold fruiting species [Re: Solipsis]
#26383069 - 12/15/19 10:24 AM (4 years, 2 months ago) |
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How long are you letting them sit for a spore print?
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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