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InvisibleSpunkyMonkey88
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Re: Question pertaining to cloning on agar EDIT: Pics [Re: verum subsequentis]
    #26385128 - 12/16/19 01:12 PM (4 years, 1 month ago)

Quote:

verum subsequentis said:
That plate MAY be clean but has a few "red flags". Looking at cultures on agar is something that takes a little practice. Important things to look for (other than obviously visible contams" are consistent/ organized growth. On your plate, if you look closely, you'll notice the myc going from "flat" to "puffy" in quite a few places. This would qualify as inconsistent/ unorganized growth.

It's not impossible to get clean cultures on you first plate with cloned tissue but, as a rule of thumb, it's good to do another transfer. Specially when the culture is showing probable signs of underlying contams.

I'd take a transfer or three from the most organized leading edges to new plates. You can put the rest of the plate to grain or LC if you want but i wouldn't promise the results would be good.




Thanks for the response, I am going to do just that.

This is the plate as of today. I was thinking of taking a couple sectors from between 6:00 & 9:00... what do you think?


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Offlineverum subsequentis
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Re: Question pertaining to cloning on agar EDIT: Pics [Re: SpunkyMonkey88]
    #26385138 - 12/16/19 01:16 PM (4 years, 1 month ago)

I'd go with 5-6 personally but that's just me.


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InvisibleSpunkyMonkey88
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Re: Question pertaining to cloning on agar EDIT: Pics [Re: verum subsequentis]
    #26385141 - 12/16/19 01:17 PM (4 years, 1 month ago)

Will do:thumbup:


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