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OfflineDodex
Stranger
Registered: 12/13/19
Posts: 2
Last seen: 4 years, 2 months
Contamination or just unusually rapid growth?
    #26380265 - 12/13/19 10:05 PM (4 years, 2 months ago)

Hey guys, really trying to get my grow to work after several set backs due to the constant learning of, well everything. I have decided to work on agar and made myself a make shift glovebox to do so. All photos I will refer to are from [link removed]*

I put spores from a syringe onto a depleted medium (LB+amp, no glucose, I had an identical one with sucrose added too), one meant for bacteria, one was distributed by excess solution, one by innoc loop. I got that monolayer of growth only 18 hours after incubation at 81 degrees on both plates, Glu+ was faster and more growth obviously . I had a control plate, no growth, however it was not in contact with anything that touched the syringe.

After plate came to near confluency quite quickly (2-3 days) I selected tissue samples and put them onto a peroxidated MAE medium (red). These were two colonies that have become tissue in the past 24 hours (photos two and three)

Do these look like contamination? Or am I good...I am honestly not sure.

The growth was white, just not a very dense white (due to trying to find nutrients on depleted plate?) The 'mycelium' looked quite tomantose, and I noticed on the Glu + plate the spores created a yellow wasteland as
all the colonies convened on the area. My other plate (low nutrient) has since become quite yellow since several spore colonies took over entire surface, so I'm assuming this is mycelium becoming nutrient depleted?

Let me know what y'all think!



Edited by Dodex (12/13/19 11:22 PM)


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Offlineattednev
Do Less


Registered: 09/10/19
Posts: 122
Last seen: 5 months, 19 days
Re: Contamination or just unusually rapid growth? [Re: Dodex]
    #26380292 - 12/13/19 10:28 PM (4 years, 2 months ago)

no one is going to click that link. You're safer loading you pics here anyways.


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Offlinespiritlands
 User Gallery


Registered: 06/21/10
Posts: 1,616
Loc: Flag
Last seen: 2 months, 14 days
Re: Contamination or just unusually rapid growth? [Re: attednev]
    #26380315 - 12/13/19 11:00 PM (4 years, 2 months ago)

Thick translucent Mycelium like that that looks like mold.


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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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OfflineDodex
Stranger
Registered: 12/13/19
Posts: 2
Last seen: 4 years, 2 months
Re: Contamination or just unusually rapid growth? [Re: attednev]
    #26380339 - 12/13/19 11:22 PM (4 years, 2 months ago)

Good point, fixed it. Too used to Reddit


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OfflineNeowynd8
 User Gallery
Registered: 06/12/16
Posts: 277
Last seen: 7 months, 20 days
Re: Contamination or just unusually rapid growth? [Re: Dodex]
    #26380351 - 12/13/19 11:38 PM (4 years, 2 months ago)

Doesn't look good.


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Offlineattednev
Do Less


Registered: 09/10/19
Posts: 122
Last seen: 5 months, 19 days
Re: Contamination or just unusually rapid growth? [Re: Neowynd8]
    #26380373 - 12/14/19 12:03 AM (4 years, 2 months ago)

It looks a bit like pin mold. Sorry dude


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