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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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OfflinePrietenul
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Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 4 months
Agar bottle opened for a second. Problem?
    #26379604 - 12/13/19 04:07 PM (4 years, 1 month ago)

Turned the cap while I was distracted and heard the hissing of entering air...immediately closed it again. :/

Would you consider that a problem or is a little bit of air ok? There was nothing dusty or anything around me.

I'm just asking, cause I don't want to spoil the 20 petri dishes


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OfflineNeowynd8
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Registered: 06/12/16
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Re: Agar bottle opened for a second. Problem? [Re: Prietenul]
    #26379617 - 12/13/19 04:11 PM (4 years, 1 month ago)

IF unsterile air went in, I'd consider it contaminated. You can always just sterilize it again. Was it heated and compressed? Are you sure it wasn't leaving the container?


Edited by Neowynd8 (12/13/19 04:13 PM)


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OfflinePrietenul
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Re: Agar bottle opened for a second. Problem? [Re: Neowynd8]
    #26379656 - 12/13/19 04:25 PM (4 years, 1 month ago)

I prepared it yesterday and let it in the fridge over night. Today I took it from there and on the way to the microwave I slightly turned the cap while talking to my girl.

I heard a soft "hisss" and closed it immediately. Of course I was not in a sterile environment, although it was in the air in the middle of a quite clean room.

If I sterilize it again, won't it caramelize? It was treated according to BODs Agar TEK


Edited by Prietenul (12/13/19 04:26 PM)


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OfflineElViajero
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Re: Agar bottle opened for a second. Problem? [Re: Prietenul]
    #26379669 - 12/13/19 04:29 PM (4 years, 1 month ago)

i've had air before enter an agar bottle, and after pouring it confirmed it wasnt contaminated.

it can be risky tho...


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OfflinePrietenul
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Registered: 11/22/19
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Loc: Germination
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Re: Agar bottle opened for a second. Problem? [Re: ElViajero]
    #26379674 - 12/13/19 04:30 PM (4 years, 1 month ago)

So you risked it. I think I will, too.


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OfflineNeowynd8
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Re: Agar bottle opened for a second. Problem? [Re: Prietenul]
    #26380338 - 12/13/19 11:22 PM (4 years, 1 month ago)

Caramelization is a possibility. I think some recipes are more prone than others. I've never really seen or read about this actually happening though. Check this out https://www.shroomery.org/forums/showflat.php/Number/26225674 He kinda attempted to do it and it still worked fine.


Edited by Neowynd8 (12/13/19 11:26 PM)


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OfflinePrietenul
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Registered: 11/22/19
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Re: Agar bottle opened for a second. Problem? [Re: Neowynd8]
    #26380680 - 12/14/19 05:45 AM (4 years, 1 month ago)

That is good to know. Makes totally sense what the guys say in that thread. Thanks for the info!


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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


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