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InvisibleTeaforTwo
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I'm a teapot


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Registered: 09/22/19
Posts: 1,005
Loc: Psychonaut City, Mile Hi!
Re: Bacteria galore, forge on or start over? [Re: YogiBear]
    #26382889 - 12/15/19 08:23 AM (4 years, 1 month ago)

Quote:

YogiBear said:




LOL! that needs submission in the new smileys thread:awesomenod:


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OfflineSpeckOnEarth
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Registered: 11/21/19
Posts: 13
Loc: Alta California
Last seen: 2 years, 20 days
Re: Bacteria galore, forge on or start over? [Re: StygianKnight]
    #26395649 - 12/22/19 01:44 PM (4 years, 1 month ago)

This is what I'm hoping for. I did foul up by not leaving any room for the race in that first round of plates. the other night I worked on another ten, used better inoculation loop technique on some and left more room. Also I did some by putting a couple of drops on (learned the hard way that syringes prefer to squirt than drip), tilting and letting the drips streak themselves. That technique is going to take some more practice for sure.

Going to try and go straight from spores next. Feeling pretty encouraged. Just wondering, should I sterilize some water and rehydrate spores from prints or just use the inoculation loop to draw dry spores across the agar?

Thanks all!


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OfflineGrowtech
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Registered: 11/09/19
Posts: 165
Last seen: 2 years, 5 months
Re: Bacteria galore, forge on or start over? [Re: SpeckOnEarth]
    #26395783 - 12/22/19 03:12 PM (4 years, 1 month ago)

As long as the spores aren’t old, you can go straight spore to agar.  Doesn’t hurt to use a slightly less ratio of agar so it’s a little softer.


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