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OfflinePrietenul
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Registered: 11/22/19
Posts: 379
Loc: Germination
Last seen: 3 years, 4 months
Are these different strains or just one with extremely rhizo?
    #26375621 - 12/11/19 05:37 PM (4 years, 1 month ago)

Hi,

I have some dishes which look like this:



Now, I don't know if this is one culture or many. If I transfer from this one, should I try to just take one of those "fingers" or take more of them together?

Any suggestion is appreciated


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OfflinePrietenul
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Registered: 11/22/19
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Re: Are these different strains or just one with extremely rhizo? [Re: Prietenul]
    #26375651 - 12/11/19 05:55 PM (4 years, 1 month ago)

In fact, should I isolate further on this?


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OfflineJohnRainy
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Registered: 07/09/19
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Re: Are these different strains or just one with extremely rhizo? [Re: Prietenul]
    #26375658 - 12/11/19 05:58 PM (4 years, 1 month ago)

That's a good example to study.

I hope some of the resident actual mycologists can clarify what exactly is going on there.

Like those two spaces on the way to 12 o'clock.  Does that count as sectoring? 

And the 3 and 9 o'clock fuzz part.  You can see it moving towards rhizomorphic appearance, and even the top and bottom dominant looking areas emerged from fuzz.

I like it when the plate is uniform throughout.  I don't have to think and wonder about all that.

Looks like good mycelium anyways.  It is my inclination to transfer tiny parts where you think it's best to get a more uniform growth on the next plate, and maybe use the rest of the plate for grains if you're needing to do that.


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OfflineJohnRainy
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Re: Are these different strains or just one with extremely rhizo? [Re: Prietenul]
    #26375660 - 12/11/19 05:59 PM (4 years, 1 month ago)

Quote:

Prietenul said:
In fact, should I isolate further on this?




Yes


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OfflinePrietenul
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Registered: 11/22/19
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Re: Are these different strains or just one with extremely rhizo? [Re: JohnRainy]
    #26375677 - 12/11/19 06:06 PM (4 years, 1 month ago)

Thanks.

Do you have an idea, why there is this opaque/diffuse part in the middle? Shouldn't be any contam, right?

I guess I'll just transfer a tiny part from each top and bottom. And there I'll try to take myc from the "finger" that is farthest away from the center. Right?


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OfflinePrietenul
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Re: Are these different strains or just one with extremely rhizo? [Re: Prietenul]
    #26375687 - 12/11/19 06:10 PM (4 years, 1 month ago)

These are two more (quite similar):






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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Are these different strains or just one with extremely rhizo? [Re: JohnRainy]
    #26375704 - 12/11/19 06:15 PM (4 years, 1 month ago)

I wud call that sectoring.
Jst to clarify, there are many many strains there. Two spores germinate, send out hyphae, the two hyphae mate creating one strain.

If it's free of contamination (i suspect they are) then it's kinda up to you what you wanna do. You cud knock up a jar with the entire plate. You cud haphazardly narrow down genetics by making transfers in an attempt to get a culture that gives you uniform growth on a plate. Do as you wish homie.


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OfflinePrietenul
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Re: Are these different strains or just one with extremely rhizo? [Re: Feasoghorm]
    #26375716 - 12/11/19 06:21 PM (4 years, 1 month ago)

My goal is the holy grail, getting some single sector isolates that are great fruiters. :grin:

Until now, I thought that these might be rhizomorphic growth. Especially, because this is the 6th transfer and I was hoping to get close to single sectors soon. But looks like I was wrong....


Edited by Prietenul (12/11/19 06:21 PM)


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OfflineJohnRainy
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Re: Are these different strains or just one with extremely rhizo? [Re: Prietenul]
    #26375718 - 12/11/19 06:22 PM (4 years, 1 month ago)

I have no properly informed idea why the mycelium is fuzzy off the wedge and then turns rhizomorphic

I toying with the speculation that perhaps mating pairs are forming within the fuzz.  I hope they are enjoying themselves spectacularly as they hop from partner to partner, looking for the one that really makes them feel like living.

You've got the same idea as me when it comes to where to take transfers.

The most interesting strand to me is on your bottom plate in the later post.  The thick string on it's way to two o'clock.  Looks powerful.

But this is all a guessing game to me at this point.


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: Are these different strains or just one with extremely rhizo? [Re: Prietenul]
    #26375728 - 12/11/19 06:26 PM (4 years, 1 month ago)

I guess keep transferring. I've had monocultures show up 1st transfer from spore, and sometimes never.
Maybe post a pic in the general cult discussion and/or all things agar threads and inquire about your goal. Perhaps one of the big strappers will chime in and teach us something new.


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OfflinePrietenul
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Re: Are these different strains or just one with extremely rhizo? [Re: JohnRainy]
    #26375730 - 12/11/19 06:27 PM (4 years, 1 month ago)

Yeah, that's a nice thought. They're having their little shroomorgy there :grin:

I'll let them grow out a little bit more and take 2-3 transfers from each. Man I'm using too many petri dishes, this is getting really expensive. Thinking about using the glass dishes...


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OfflinePrietenul
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Registered: 11/22/19
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Re: Are these different strains or just one with extremely rhizo? [Re: Feasoghorm]
    #26375733 - 12/11/19 06:28 PM (4 years, 1 month ago)

That's good idea. I'll try that too.


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