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Offlineogdoadtoad
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Registered: 12/04/18
Posts: 9
Last seen: 3 years, 9 months
Agar contams ?
    #26374933 - 12/11/19 11:59 AM (4 years, 1 month ago)

Started doing a batch of Potato dextrose agar need help identifying possible contams. These are the plates after 2 weeks. Are they savable?
Plate 1




Plate 2




Plate 3




Edited by ogdoadtoad (12/11/19 12:26 PM)


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OfflineJohnRainy
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Registered: 07/09/19
Posts: 1,244
Last seen: 3 years, 11 months
Re: Agar contams ? [Re: ogdoadtoad]
    #26374941 - 12/11/19 12:02 PM (4 years, 1 month ago)

You want to identify those contams?  Like, know their species?


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OfflineMrBovineJoni
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Registered: 11/28/19
Posts: 264
Last seen: 13 days, 16 hours
Re: Agar contams ? [Re: JohnRainy]
    #26374952 - 12/11/19 12:05 PM (4 years, 1 month ago)

All of these are super duper contaminated - bacterial (possibly mixtures of multiple bacteria)


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Offlineogdoadtoad
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Registered: 12/04/18
Posts: 9
Last seen: 3 years, 9 months
Re: Agar contams ? [Re: MrBovineJoni]
    #26374994 - 12/11/19 12:27 PM (4 years, 1 month ago)

Can i replant the agar?


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Offlinetrubblesome
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Registered: 11/09/19
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Re: Agar contams ? [Re: ogdoadtoad]
    #26375001 - 12/11/19 12:30 PM (4 years, 1 month ago)

no, i don't see any mycelium to transplant


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OfflineMrBovineJoni
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Registered: 11/28/19
Posts: 264
Last seen: 13 days, 16 hours
Re: Agar contams ? [Re: trubblesome]
    #26375014 - 12/11/19 12:41 PM (4 years, 1 month ago)

Nope - those petri dishes are a lost cause. Throw out and retry with new ones (doubt that these plastic ones can be cleaned, sterilised again and reused).


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Offlineogdoadtoad
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Registered: 12/04/18
Posts: 9
Last seen: 3 years, 9 months
Re: Agar contams ? [Re: MrBovineJoni]
    #26375023 - 12/11/19 12:49 PM (4 years, 1 month ago)

The spore syringe was at least 2 years old and kept at room temperature, could that be the culprit? Other than that i followed every instruction i could find to sterilize the equipment and environment used.


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Offlinesan pedro guy
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Registered: 10/22/17
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Re: Agar contams ? [Re: ogdoadtoad]
    #26375029 - 12/11/19 12:54 PM (4 years, 1 month ago)

how did you put the spores on the plates? Did you do it in a sab?

Doesn’t look like you streaked them at all. Looks like they were just squirted with 2cc’s or more!

If you have any spore solution left try to put less than a drop on 1 plate and streak it in an s shape...


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Noob Grow Along 2022


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InvisibleWhyterye
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Registered: 02/23/18
Posts: 1,218
Loc: Colorado Flag
Re: Agar contams ? [Re: ogdoadtoad]
    #26375031 - 12/11/19 12:56 PM (4 years, 1 month ago)

:rasta:


Edited by Whyterye (08/05/20 06:41 PM)


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Offlineogdoadtoad
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Registered: 12/04/18
Posts: 9
Last seen: 3 years, 9 months
Re: Agar contams ? [Re: san pedro guy]
    #26375100 - 12/11/19 01:37 PM (4 years, 1 month ago)

It was all done inside a SAB, on two plates with a zig zag movement using a inoculation loop and one was a squirt to see what would happen.


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