Hello guys, I've been using these pp5 containers and I'm in love with them, I think they are the holy grail of no pour agar pp5 containers.

Features:

• No-pour compatible.
  
• Great visibility. The visibility window is the own lid. See through the lid in 2D as you would do with disposable petri dishes.
  
• Unmodded, no need to poke holes for GE or use filter materials, thanks to the screw top design. Contaminated air cannot travel through obscure pathways in all circumstances!
  
• Sturdy plastic, plastic won't warp in the PC that easily
  
• No boil-over risk in the PC
  
• Easy labeling on the sides. See the suggestions bellow.
  
• Pseudo-slants, see the suggestion bellow.
  
  

The closest thing for a no-pour polypropylene container to the visibility of a petri dish and without having to lose money and time with parafilm

I achieved very little/no condensation in most of the containers after sterilization, minimal or no pooling when following this tek.

You will need 3 things for this tek.

First of all the containers. A second source Third source (12$ for 20 BUT THEY HAVE DEFECTIVE LID UNFORTUNATELY)



You will need this tall container or something similar! This one is a chocolate Christmas container. (11.25 inches tall - 3 inches diameter)



And you will need a BIG sterilizer(not really, see the mason jar method in the suggestions bellow), I'm using a AA75X. I know for noobs this might be a big step back, but oh well, you can't have everything. You need a big sterilizer because the tall container is 11 inches tall.EDIT: Condensation or pooling will evaporate in aprox 2 weeks with the lid loose, see the suggestions page bellow).

Pour the agar in the containers, i love it because 100 ml of liquid agar can pour 10 containers. So roughly 10 ml of agar per container, easy. (EDIT: I use now 20ml of agar per container, easier to do whole wedge transfers.)



This is how deep it would look roughly 10ml of agar per container.



Let them cool completely before screwing the lids (screw the lids slightly loose).

Put the tall container upside down and put the holy grail containers inside the large container, they fit perfectly inside. This tall container in particular can hold in the inside up to 10 HG containers .



I place the tall container inside the sterilizer, my sterilizer can hold up to 10 of these tall containers, so i can sterilize 100 HG containers in a single shot. Sterilize them at 17 psi for 1 hour (EDIT: since in this tek we sterilize the plates inside a mason jar or any other outer container, these plates seems to require more sterilization time to achieve the proper temperature inside the outer container, 1 hour appears to be enough). Turn off the sterilizer and walk away, do not release the steam valve, let it slowly go to 0 psi, when is at 0 psi start the counter for 1 hour, once the sterilizer is a little more cooler (still will burn your hand if you touch it), remove the lid of the PC. DON'T LEAVE the PC to cool all night! this will cause pooling in your HG containers! Take out the tall container and put it in a room with stable temperature, not your bedroom with air conditioner or heater causing big temp fluctuations!

Remove the upside down part of the tall container and ensure to stack all HG containers vertically on top of each other correctly, this is essential, very similar like when you pour agar in front of the hood or SAB and the petri that is on top is the one that get most of the condensation but the plates that are bellow don't get any condensation. (You can do this in open air, don't worry about contams.)

13 plates stacked in this picture:



Put the upside down part of the tall container back on



Wait until they cool completely, come back and this should be the result:

1.- The HG container at the top with most of the condensation (most of the condensation will go away with time anyway).



2.- less condensation



3.- even less



4.- Almost none



5.- No condensation



6.- Nope



7.-

8.-

9.-

Some pictures of pan cyan mycelium growing happily and with great visibility. I'll add more pictures later.



GREAT visibility, i would say 8.5 from 10, comparable to the visibility of a real petri dish. Eatyualive took pictures with better lighting than what i posted above:

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eatyualive said:
So far loving the plates!

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Awesome visibility for a pp5

Cons.

• twice as expensive as the glad mini rounds (6$ for 8 containers) vs holy grail containers (12$ 15.5$ for 8 containers). NEW SOURCE! This source are selling them 20 hg for 12$ BUT THEY HAVE DEFECTIVE LID UNFORTUNATELY!
  
• Another Con is that is not compatible with tiger drop. But if you want to really use the tiger drop, you could use the holy grail containers for all the HG to HG transfers and the pasty plates for the final A2G by tiger dropping the wedge
  
• Another Con is that they are 2.5 inches wide compared to a regular petri dish which is 3.5 inches wide.
  

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setarcoS said:
So you inoculate by removing the lids?

Love your enthusiasm. I was waiting for the secret trick that invalidates using micropore tape/making holes in the pp container.

If working with agar slurry (~0.2% agar) then I can often get away with only using a needle through some small hole in the container. Contamination vectors are thus massively reduced. Curious to hear your thoughts

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SpunkyMonkey88 said:
Nevermind u did link them sorry..

I wonder if you just vent the PC as soon as they're done, take them out steaming hot and stack them as high as possible, if you'd have the same results reducing condensation as using your xmas tin..

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SpunkyMonkey88 said:
I love it but where the fuck am I going to find a "large container " like that nutcracker thing you used 😂 I wonder If it's really necessary..

Awesome write up I'll have to look for those pp5s, can you link them?

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cronicr said:
Beautiful work my friend

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Thank you! I did it thinking in you my friend.

Also, if you pour a lot of agar into these holy grail containers, i bet they could function like agar slants. It will take a lot of time for them to dry out, as the container is deep enough. Not as much as real agar slants but quite a lot, i will test this in the future.

There are only 8 packs left in amazon Glad i bought 100 before publishing this.

The problem is that I only have 3 of those tall containers

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san pedro guy said:
since most of us don’t have a giant nut cracker do you think anything on top might work the same? Won’t fit in my pc for sure but maybe after it’s done cooking, I cover with something for same result?

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Hmmm i might have to do some tests once the infrared thermometer arrives. I might test it with 10 Holy Grail containers without the large/tall container, and once at 0PSI measure the temperature of the pressure cooker to pull the containers out at the right temp, then stack them vertically again and cover them with a bag or something.  But idk tho, insulation of the metal container probably plays a major role in avoiding condensation/pooling, how many HG containers do you think you can stack up vertically inside your small PC? What PC model do you have?

I don't know, i think using this method justifies buying a AA75X if you want to stop pouring agar to petris or continue using poor visibility no pour modified containers with holes and filters and condensation/pooling. Besides that you can always do more grain jars or grain mycobags. an AA75X is always a good buy. My two cents.